[Histonet] LCM question
Hi, I'm interested in LCM-Systems. I'd like to know, if there are systems, that can transfer cut regions of the donor-section on to other slides for further staining like FISH. Until now I've found only internet-information about systems for harvesting tissue or cells for further molecular testing, where the tissue is desintegrated after harvesting. Perhaps someone can provide some information like an weblink. Thanks Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] how to make crashed ice?
Hi! I think this is a rather basic question ;-), but I'm looking for practical advice. We are going to try the OSNA-test for sentinel nodes. The application needs a pot with crashed ice while desintegrating the tissue with a mixer. So over the day this should be four to six litre ice, if we have to take fresh ice for each time, a group of sentinels has to be worked up. What is a practical way to make crashed ice in the lab? Thanks for your answers in advance! Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] how to make crashed ice?
Hi gudrun, Place the ice - ice cubes works best in a plastic bag, wrap in a towel and bash with a heavy object like a hammer. You can also use Jamie Oliver's trick - put ice cubes in a cloth tea towel, , bring the 4 corners together, tie them in knot, and then hit the parcel on the edge of your work surface until the ice is the sze you need hope this helps On Sat, Aug 7, 2010 at 12:12 PM, Gudrun Lang gu.l...@gmx.at wrote: Hi! I think this is a rather basic question ;-), but I'm looking for practical advice. We are going to try the OSNA-test for sentinel nodes. The application needs a pot with crashed ice while desintegrating the tissue with a mixer. So over the day this should be four to six litre ice, if we have to take fresh ice for each time, a group of sentinels has to be worked up. What is a practical way to make crashed ice in the lab? Thanks for your answers in advance! Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] how to make crashed ice?
Hello, It is very inexpensive, a few dollars, to buy a counter-top ice crusher, that a professional bartender might use to crush ice for drinks. Can be bought at most any store. We crush ice every day on a daily basis to create a small tub of ice we need for reagents. Only the size of like a small kitchen food processor. Just add ice cubes and crush them in a few seconds without a hammer and the mess. Ray PhenoPath Labs Seattle, WA - Original Message - From: louise renton louise.ren...@gmail.com To: gu lang gu.l...@gmx.at, Histonet@lists.utsouthwestern.edu Sent: Saturday, August 7, 2010 3:24:17 AM Subject: Re: [Histonet] how to make crashed ice? Hi gudrun, Place the ice - ice cubes works best in a plastic bag, wrap in a towel and bash with a heavy object like a hammer. You can also use Jamie Oliver's trick - put ice cubes in a cloth tea towel, , bring the 4 corners together, tie them in knot, and then hit the parcel on the edge of your work surface until the ice is the sze you need hope this helps On Sat, Aug 7, 2010 at 12:12 PM, Gudrun Lang gu.l...@gmx.at wrote: Hi! I think this is a rather basic question ;-), but I'm looking for practical advice. We are going to try the OSNA-test for sentinel nodes. The application needs a pot with crashed ice while desintegrating the tissue with a mixer. So over the day this should be four to six litre ice, if we have to take fresh ice for each time, a group of sentinels has to be worked up. What is a practical way to make crashed ice in the lab? Thanks for your answers in advance! Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] de-wax without organics
Hi, I am curious what temperature and percent detergent you are using. Also which detergent did you use? Some are different than others. Do you have a list of special stains you have tried? I am sure this will probably work great, I just think that it is conceivable for different labs using different detergents, different concentrations and different temperatures to get different results with some stains. This is a really cool subject and if you could compile the information into an article I could find it a home in the Paraffin Press for the Connecticut Society. Just in case you are interested. Thanks, Amos Message: 5 Date: Fri, 6 Aug 2010 19:13:17 + From: Goins, Tresa tgo...@mt.gov Subject: [Histonet] de-wax without organics To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID: ca4df32ed505d94bb55e95487d8e984104f...@doaisd5205.state.mt.ads Content-Type: text/plain; charset=us-ascii We have run some preliminary tests on de-waxing slides using warm detergent solutions rather than xylene or xylene substitutes. So far the pathologists see no difference between the HE stained slides treated with detergent vs. the usual xylene to alcohol to water route; the cellular detail is equivalent and the dye reactions appear identical. We have also run comparisons for several special stains and IHC slides with good results. I would greatly appreciate hearing from anyone who is aware of reasons why this is a bad idea! It is quicker and reduces the volume of organics required by half so we are seriously considering it as an alternative protocol. Thanks in advance for your feedback. Tresa Tresa Goins Veterinary Diagnostic Lab Department of Livestock Bozeman, Montana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
