RE: [Histonet] muscle stain
Toluidine blue stains striated muscle nicely. Regards Birgitta Stephenson University of Queensland Microscopy Research Lab On Tue, 17 Aug 2010 17:18:18 -0400, Sherwood, Margaret msherw...@partners.org said: Masson Trichrome stains collagen and muscle. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Tuesday, August 17, 2010 5:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] muscle stain I am posting this for a friend. . They would like to stain muscle. The eosin and hematoxylin stain gives us too much information since it stains the nucleus separately from the cytoplasm and confuses the image analysis software. What we need is the measurement of the cell diameter only, a simpler stain. Thanks for any info you can give me. Deon Simon Any help you can give her on how to measure or a stain will be very much appreciated. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Birgitta Stephenson bstep...@fastmail.fm -- http://www.fastmail.fm - A no graphics, no pop-ups email service ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome alignment
This is a learned histology skill, but there are some devices which can be used to align different microtomes available, and you can test your adjustments with blank blocks. Also newer microtomes have a device on the block holder that will align the position in the X-Y axis in the zero position. Joelle Weaver From: sharon.davis-dev...@carle.com To: histonet@lists.utsouthwestern.edu Date: Tue, 17 Aug 2010 14:16:26 -0500 Subject: [Histonet] Microtome alignment We are having a continuing issue of too much tissue being cut off when facing off a block for recuts. We have tried a couple of different methods for aligning our microtomes without much success. Does anyone out there have any advice on how to properly align them and what tool to use? Also, how often do you perform this re-alignment? The majority of our microtomes are older so more wear and tear and things move out of place more often. Any help or suggestions will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome alignment
A while back I remember someone suggestion something like a right angle device that carpenters use. It's basically just a piece of metal that is a right angle triangle that you put up against the chuck and on the knife mount. Then you align the chuck so it is a a right angle to the knife mount. It looks like this:l\ l \ l\ This is my best attempt at computer drawing. l __ \ I don't thing they cost very much, much less that $700. Paula :-) On Tue, Aug 17, 2010 at 8:05 PM, WILLIAM DESALVO wdesalvo@hotmail.comwrote: Since you have older microtomes, I suggest using an alignment block at each microtome instead of purchasing the alignment tools. The tools can be found on the web ttp://www.grale.com.au/products/view/804 , but they can be expensive (as much as $700.00 each). If you have more than one manufacturer for your microtomes, you will need to purchase one for each brand. Try using your largest embedding mold and make a blank block for each microtome. This can bee done first thing each morning. Use the block to align the chuck each morning before cutting. If you see drift throughout the day, add one or more checks during the day. Making a fresh block each day gives you a good standard and keeps the variation down. I also suggest you look at your embedding method and make sure you have a standardized procedure for all tissue types for orientation of tissue and exact placement in the mold. Embed your tissue on one plane with as little paraffin as possible on the bottom of the mold. Reducing variation at embedding will greatly assist you in reducing the amount of facing required to start producing sections and also reduce the need to align the chuck to the block/tissue. William DeSalvo, B.S., HTL(ASCP) Chair, NSH QCC Prodcution Manager, Sonora Quest Laboratories From: sharon.davis-dev...@carle.com To: histonet@lists.utsouthwestern.edu Date: Tue, 17 Aug 2010 14:16:26 -0500 Subject: [Histonet] Microtome alignment We are having a continuing issue of too much tissue being cut off when facing off a block for recuts. We have tried a couple of different methods for aligning our microtomes without much success. Does anyone out there have any advice on how to properly align them and what tool to use? Also, how often do you perform this re-alignment? The majority of our microtomes are older so more wear and tear and things move out of place more often. Any help or suggestions will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Paula Sicurello 6 of 6 Duke Healthcare System EM Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome alignment
Yes, these used to be available from Newcomer supply, but I think they are now de-funked. Try a google search perhaps? Joelle Date: Wed, 18 Aug 2010 07:00:23 -0400 From: pat...@gmail.com To: wdesalvo@hotmail.com Subject: Re: [Histonet] Microtome alignment CC: histonet@lists.utsouthwestern.edu A while back I remember someone suggestion something like a right angle device that carpenters use. It's basically just a piece of metal that is a right angle triangle that you put up against the chuck and on the knife mount. Then you align the chuck so it is a a right angle to the knife mount. It looks like this: l\ l \ l \ This is my best attempt at computer drawing. l __ \ I don't thing they cost very much, much less that $700. Paula :-) On Tue, Aug 17, 2010 at 8:05 PM, WILLIAM DESALVO wdesalvo@hotmail.comwrote: Since you have older microtomes, I suggest using an alignment block at each microtome instead of purchasing the alignment tools. The tools can be found on the web ttp://www.grale.com.au/products/view/804 , but they can be expensive (as much as $700.00 each). If you have more than one manufacturer for your microtomes, you will need to purchase one for each brand. Try using your largest embedding mold and make a blank block for each microtome. This can bee done first thing each morning. Use the block to align the chuck each morning before cutting. If you see drift throughout the day, add one or more checks during the day. Making a fresh block each day gives you a good standard and keeps the variation down. I also suggest you look at your embedding method and make sure you have a standardized procedure for all tissue types for orientation of tissue and exact placement in the mold. Embed your tissue on one plane with as little paraffin as possible on the bottom of the mold. Reducing variation at embedding will greatly assist you in reducing the amount of facing required to start producing sections and also reduce the need to align the chuck to the block/tissue. William DeSalvo, B.S., HTL(ASCP) Chair, NSH QCC Prodcution Manager, Sonora Quest Laboratories From: sharon.davis-dev...@carle.com To: histonet@lists.utsouthwestern.edu Date: Tue, 17 Aug 2010 14:16:26 -0500 Subject: [Histonet] Microtome alignment We are having a continuing issue of too much tissue being cut off when facing off a block for recuts. We have tried a couple of different methods for aligning our microtomes without much success. Does anyone out there have any advice on how to properly align them and what tool to use? Also, how often do you perform this re-alignment? The majority of our microtomes are older so more wear and tear and things move out of place more often. Any help or suggestions will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Paula Sicurello 6 of 6 Duke Healthcare System EM Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: muscle stain
There is an immunofluorescence technique that uses wheat germ agglutinin (WGA) conjugated to a fluorophore (usually FITC). WGA binds only to the cell periphery, leaving you with beautiful traces of the outside of each cell. The cell area is then simple to analyze with ImageJ or any other software program. It's often used to measure cardiomyocyte size in hypertrophy studies. I can provide a protocol if requested. If you have access to this article, there is a detailed description of the technique and great images: Regional changes in myocyte structure in model of canine right atrial hypertrophy. Am J Physiol Heart Circ Physiol 267: H1279-H1287, 1994. P. C. Dolber, R. P. Bauman, J. C. Rembert and J. C. Greenfield Jr. If not, here is a freely accessible publication. Figure 9, panel B shows what the result looks like. Cardiomyocyte GATA4 functions as a stress-responsive regulator of angiogenesis in the murine heart. J Clin Invest. 2007; 117(11):3198. http://www.jci.org/articles/view/32573/figure/9 Andrea Marion Graduate Student University of Illinois - Chicago amario3 at uic dot edu To: histonet@lists.utsouthwestern.edu Subject: [Histonet] muscle stain I am posting this for a friend. . They would like to stain muscle. The eosin and hematoxylin stain gives us too much information since it stains the nucleus separately from the cytoplasm and confuses the image analysis software. What we need is the measurement of the cell diameter only, a simpler stain. Thanks for any info you can give me. Deon Simon Any help you can give her on how to measure or a stain will be very much appreciated. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: Re-thinking Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!!
Reminder... these 'please send money' or 'please send your bank acct information so that I can deposit money into your acct' pleas are ALWAYS scams. They have been for years. If a trusted 'friend' was truly stranded in a foreign country, they'd borrow a phone and call home. If a long lost relative died and left you money in another country, it would be a lawyer contacting you via telephone or letter, and definitely not through an email message. Best advice... don't even open these messages up. They're bogus, and could be carrying viruses, worms, etc. themselves. Being on a listserv like histonet, we're prone to receive a lot of these email fraud attempts. Ignore them. Jan Shivers - Original Message - From: Akemi Allison akemiat3...@yahoo.com To: histonet@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu; Valantou Grover vgro...@polysciences.com Sent: Tuesday, August 17, 2010 8:24 PM Subject: Re: Re-thinking Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! Sorry Histonetters, but this needs to be cleared up! OK, Jerry Santiago, are you alive and well in FL or the UK in trouble, or is this another ridiculous scam I know how well connected you are, so one of your colleagues should know... Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Aug 17, 2010, at 1:47 PM, Eric Hill wrote: Hi, My wife got scammed with something almost the same a month ago, less detailed message in hers though! They got into her gmail and deleted all her contacts and tried to delete all her most recent emails (they forgot to clear out her trash though, so she got most back). Don't fall for it! -Eric Research Tech 1 Program in Memrane Biology Mass Genreal Hospital On Aug 17, 2010, at 4:37 PM, Akemi Allison wrote: I am rethinking this email since I read it over again. The scam email I received asked for money ($1,800) to be sent to England via Western Union. I am not sure about this since this supposed Jerry is asking for just a response email. I would still be very cautious! The Idaho news said the lady received a yahoo request (same as mine) asking for email address, password, country of origin, mothers maiden name, ect. Once the perpetrator had the information, they immediately extracted all her information and cleaned her bank accounts out. This was a business computer account and it shut down her business! Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Aug 17, 2010, at 1:20 PM, Valantou Grover wrote: Thanks, I deleted the message, now I am going to run a anti virus program. Valantou ** * Visit us at the 36th Annual National Society for Histotechnology Convention - September 26-28, Seattle, WA - BOOTH 126-128 * Polysciences/Bangs Laboratories, Inc. will be hosting the 2010 Latex Course in Orlando, FL at the Grand Floridian Resort from 10/3 – 10/5. Please enquire at i...@bangslabs.com for additional details! Phone: 800-387-0672, 317-570-7020. * From: Akemi Allison [mailto:akemiat3...@yahoo.com] Sent: Tuesday, August 17, 2010 4:14 PM To: Valantou Grover Cc: histonet@lists.utsouthwestern.edu Subject: Re: Nigerian Scam ! [Histonet] FW: NEEDS URGENT HELP !!! The email you received is a Nigerian Scam. Someone was able to hack into Jerry's computer and get all his contact list! You happened to be one of his contacts. The same sort of Scam happened to me last October. Fortunately for me, I didn't have any bank account information in my computer. I have a MAC and so I went to the Apple Genius Bar and they checked my computer for any viruses. Fortunately, it is very difficult to get into a MAC. This has been on the news in Idaho and various other states. DO NOT RESPOND to any emails that want any personal information!!! You need to clean-up your computer from any viruses. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Aug 17, 2010, at 12:53 PM, Valantou Grover wrote: I am just curious, can someone check on Jerry to see if he is dead or alive, or if someone stole his phone/laptop to get this message to me. I remember the email address from Florida Society for Histotechnology, I am wondering if this is real or if someone is assuming his identity overseas, foul play, etc. Valantou Grover, Ph.D. HT/HTL(ASCP), PA, MBA Biosciences Product Line Manager Polysciences, Inc. 400 Valley Road Warrington, PA 18976 Fax: 1-800-343-3291 Phone number: 1-800-523-2575 X7418 Direct:1-215-488-7418 Cell phone:
[Histonet] thymidine kinase 1
Does anyone have experience with the rabbit monoclonal anti-thymidine kinase 1 (clone EPR3193) on FFPE tissues? i.e. antigen retrieval necessary or not? Thanks as always, Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: muscle stain
Andrea - Wow this looks great. We do a lot of cardiomyocyte staining and studying and this could be helpful. Thanks for sharing! Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. --On Wednesday, August 18, 2010 8:18 AM -0500 Andrea Marion amar...@uic.edu wrote: There is an immunofluorescence technique that uses wheat germ agglutinin (WGA) conjugated to a fluorophore (usually FITC). WGA binds only to the cell periphery, leaving you with beautiful traces of the outside of each cell. The cell area is then simple to analyze with ImageJ or any other software program. It's often used to measure cardiomyocyte size in hypertrophy studies. I can provide a protocol if requested. If you have access to this article, there is a detailed description of the technique and great images: Regional changes in myocyte structure in model of canine right atrial hypertrophy. Am J Physiol Heart Circ Physiol 267: H1279-H1287, 1994. P. C. Dolber, R. P. Bauman, J. C. Rembert and J. C. Greenfield Jr. If not, here is a freely accessible publication. Figure 9, panel B shows what the result looks like. Cardiomyocyte GATA4 functions as a stress-responsive regulator of angiogenesis in the murine heart. J Clin Invest. 2007; 117(11):3198. http://www.jci.org/articles/view/32573/figure/9 Andrea Marion Graduate Student University of Illinois - Chicago amario3 at uic dot edu To: histonet@lists.utsouthwestern.edu Subject: [Histonet] muscle stain I am posting this for a friend. . They would like to stain muscle. The eosin and hematoxylin stain gives us too much information since it stains the nucleus separately from the cytoplasm and confuses the image analysis software. What we need is the measurement of the cell diameter only, a simpler stain. Thanks for any info you can give me. Deon Simon Any help you can give her on how to measure or a stain will be very much appreciated. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Test for cell viability in paraffin section?
Would you know a techniqe to confirm cell viability in paraffin embedded tissue? Propidium and methylene blue staining are used in cell culturing to detect cell viability. Is there similar technique for detecting cell viability in tissue? This question was asked by our research director, Can you please share your opinion on this and if there is chemical test to detect vaibility of cells in a paraffin tissue? Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Test for cell viability in paraffin section?
Cornelia: After a deadly fixation, followed by a complete dehydration, clearing and paraffin wax infiltration, the only potentially surviving entities are prions. No cell will be viable after this treatment and if you get some positive results of viability with what ever test you use, I caution to believe those results. Your research director evidently is absolutely ignorant about tissue processing! René J. --- On Wed, 8/18/10, Reuel Cornelia reuel.corne...@tsrh.org wrote: From: Reuel Cornelia reuel.corne...@tsrh.org Subject: [Histonet] Test for cell viability in paraffin section? To: histonet@lists.utsouthwestern.edu Date: Wednesday, August 18, 2010, 9:55 AM Would you know a techniqe to confirm cell viability in paraffin embedded tissue? Propidium and methylene blue staining are used in cell culturing to detect cell viability. Is there similar technique for detecting cell viability in tissue? This question was asked by our research director, Can you please share your opinion on this and if there is chemical test to detect vaibility of cells in a paraffin tissue? Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Test for cell viability in paraffin section?
Hey Ruel Once we are done with them and they are all nicely embedded in paraffin wax...the cells are not viable...they are DEAD Annieinarabia Empower your Business with BlackBerry® and Mobile Solutions from Etisalat -Original Message- From: Reuel Cornelia reuel.corne...@tsrh.org Sender: histonet-boun...@lists.utsouthwestern.edu Date: Wed, 18 Aug 2010 08:55:48 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Test for cell viability in paraffin section? Would you know a techniqe to confirm cell viability in paraffin embedded tissue? Propidium and methylene blue staining are used in cell culturing to detect cell viability. Is there similar technique for detecting cell viability in tissue? This question was asked by our research director, Can you please share your opinion on this and if there is chemical test to detect vaibility of cells in a paraffin tissue? Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Needed For Vacation Coverage In GA-1 Week
MPath Search Partners has been retained to search for a Histotechnologist/Histotechnician who is available for a vacation coverage shift in Marietta, GA. Position Title: Histotechnologist/Histotechnician Shift: September 6th-10th, 2010. That’s Monday-Friday 6-8 hour days with lunch Location Environment: Physicians Office Laboratory A practice filled with state-of-the-art- equipment, and cutting edge technology Requirements: Ability to work with no supervision HT/HTL ASCP preferred Experience working with Dermatology tissue Other: This position is just a temporary position, allowing the current histotech to take a vacation. To apply: Please send information to aly...@alliedsearchpartners.com 1. Resume 2. Expected Salary 3. Hours you are available to work -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome alignment
I assume you are referring to a small carpenter's square. That will only allow you to square up the chuck from top to bottom. It would not work for left and right adjustments. Marketlab sells an alignment tool that they claim will work for multiple microtomes. I have not tried it though. It lists for $775. I have considered having a piece of steel welded to an old knife holder and just adjust the chuck until it is flat against the steel. Should work and won't cost $775. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Wednesday, August 18, 2010 7:00 AM To: WILLIAM DESALVO Cc: histonet Subject: Re: [Histonet] Microtome alignment A while back I remember someone suggestion something like a right angle device that carpenters use. It's basically just a piece of metal that is a right angle triangle that you put up against the chuck and on the knife mount. Then you align the chuck so it is a a right angle to the knife mount. It looks like this:l\ l \ l\ This is my best attempt at computer drawing. l __ \ I don't thing they cost very much, much less that $700. Paula :-) On Tue, Aug 17, 2010 at 8:05 PM, WILLIAM DESALVO wdesalvo@hotmail.comwrote: Since you have older microtomes, I suggest using an alignment block at each microtome instead of purchasing the alignment tools. The tools can be found on the web ttp://www.grale.com.au/products/view/804 , but they can be expensive (as much as $700.00 each). If you have more than one manufacturer for your microtomes, you will need to purchase one for each brand. Try using your largest embedding mold and make a blank block for each microtome. This can bee done first thing each morning. Use the block to align the chuck each morning before cutting. If you see drift throughout the day, add one or more checks during the day. Making a fresh block each day gives you a good standard and keeps the variation down. I also suggest you look at your embedding method and make sure you have a standardized procedure for all tissue types for orientation of tissue and exact placement in the mold. Embed your tissue on one plane with as little paraffin as possible on the bottom of the mold. Reducing variation at embedding will greatly assist you in reducing the amount of facing required to start producing sections and also reduce the need to align the chuck to the block/tissue. William DeSalvo, B.S., HTL(ASCP) Chair, NSH QCC Prodcution Manager, Sonora Quest Laboratories From: sharon.davis-dev...@carle.com To: histonet@lists.utsouthwestern.edu Date: Tue, 17 Aug 2010 14:16:26 -0500 Subject: [Histonet] Microtome alignment We are having a continuing issue of too much tissue being cut off when facing off a block for recuts. We have tried a couple of different methods for aligning our microtomes without much success. Does anyone out there have any advice on how to properly align them and what tool to use? Also, how often do you perform this re-alignment? The majority of our microtomes are older so more wear and tear and things move out of place more often. Any help or suggestions will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Paula Sicurello 6 of 6 Duke Healthcare System EM Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure
[Histonet] Microtome alignment
We recently purchased the new alignment tool from Source medical products that works on any microtome as it uses a leveling bubble. It is round, so it makes certain all is level from any direction. Works well and has helped us out. I have staff use it daily as it takes minimal time. Only $200. Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: muscle stain
Hi Saro and others, Our protocol is attached. It is very simple. The only trick is to remember that if you are using this for heart tissue, each section will likely contain cardiomyocytes sectioned at various angles. To accurately measure cell size/diameter, you will want to measure only cells that are neatly cross-sectioned. If you measure cells that are cross-sectioned at an angle, the measurements will be off. This is discussed in the Dolber 1994 paper I mentioned. Essentially you want to identify regions where the WGA staining is very crisp and thin, as this will identify cells that are sectioned perpendicular to their long axis. Cells that are cross-sectioned at some oblique angle will have a fuzzy border of staining along one edge of the cell. This is sometimes hard to identify (at least for me in mouse tissue), and should probably be considered as a caveat to this technique. At the very least, I think it needs to be considered and controlled for. If anyone has a better way of identifying such cells, I'd be happy to hear it. If you are using this technique on skeletal muscle (which I haven't tried), I believe it would be much simpler as the myocytes are aligned more regularly throughout the tissue, and it would be easy to orient the tissue in the block in such a way that you get perfect cross-sectioned myocytes. Wheat Germ Agglutinin-FITC staining to measure myocyte size 1. Begin with 5-8 um FFPE sections on charged slides 2. Dewax slides 3. Rehydrate slides 4. Rinse in PBS 5 minutes x 3 5. Incubate 60 minutes with 100 ug/ml WGA-FITC in PBS + 1 mM CaCl2 6. Rinse in PBS 5 minutes x 3 7. Mount with Vectashield or Vectashield + DAPI 8. Image slides, collecting images of cross-sectioned myocytes 9. Measure area inside WGA-staining for perfectly cross-sectioned myocytes using ImageJ PBS: 8.5 mM Na2HPO4, 1.5 mM KH2PO4, 150 mM NaCl, pH 7.2 WGA-FITC: Vector Labs, Burlingame, CA Product # FL-1021 Andrea Marion Graduate Student University of Illinois - Chicago amario3 at uic dot edu On Wed, August 18, 2010 8:47 am, Bascaramurty, Saro wrote: Hi Andrea, I wouldn't mind if you could email me your protocol. Thanks in advance. Saro Bascaramurty. IBD, NRC, Wpg. MB -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea Marion Sent: August 18, 2010 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: muscle stain There is an immunofluorescence technique that uses wheat germ agglutinin (WGA) conjugated to a fluorophore (usually FITC). WGA binds only to the cell periphery, leaving you with beautiful traces of the outside of each cell. The cell area is then simple to analyze with ImageJ or any other software program. It's often used to measure cardiomyocyte size in hypertrophy studies. I can provide a protocol if requested. If you have access to this article, there is a detailed description of the technique and great images: Regional changes in myocyte structure in model of canine right atrial hypertrophy. Am J Physiol Heart Circ Physiol 267: H1279-H1287, 1994. P. C. Dolber, R. P. Bauman, J. C. Rembert and J. C. Greenfield Jr. If not, here is a freely accessible publication. Figure 9, panel B shows what the result looks like. Cardiomyocyte GATA4 functions as a stress-responsive regulator of angiogenesis in the murine heart. J Clin Invest. 2007; 117(11):3198. http://www.jci.org/articles/view/32573/figure/9 Andrea Marion Graduate Student University of Illinois - Chicago amario3 at uic dot edu To: histonet@lists.utsouthwestern.edu Subject: [Histonet] muscle stain I am posting this for a friend. . They would like to stain muscle. The eosin and hematoxylin stain gives us too much information since it stains the nucleus separately from the cytoplasm and confuses the image analysis software. What we need is the measurement of the cell diameter only, a simpler stain. Thanks for any info you can give me. Deon Simon Any help you can give her on how to measure or a stain will be very much appreciated. Margaret Perry HT(ASCP) Dept of Veterinary and Biomedical services Box 2175 South Dakota State University Brookings SD 57007 605-688-5638 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Advanced Stainers
For those of you who have experience with the Bond or Benchmark XT; what do you like/dislike about them? We're looking at advanced stainers as well. Any reoccurring problems you could do without? You don't really hear about those when meeting with company reps. Thank you in advance for any feedback! Olivia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD34 positive control
I have a doctor who wants to stain some tissue with cd34. I need to know if anyone can recommend a good positive control tissue for this antibody. The staining will be done in paraffin embedded sections. Thanks in advance. Joel -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensper...@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD34 positive control
Blood vessels in tissue. Here is a website that I use for p= ositive
control list.
[1]http://www.pantomics.com/support/IHCcontr= oltissuelist.htm
There are tons of lists out t= here, this is just one.
Sarah Goebel, B.A., HT (ASCP)
H= istotechnician
XBiotech USA Inc.
8201 East Ri= verside Dr. Bldg 4 Suite 100
= Austin, Texas 78744= /em
Original Message
Subject: [Histonet] CD34 positive control
From: Joel Reichensperger [2]jreichensper...@siumed.edu
Date: Wed, August 18, 2010 9:05 am
To: Histonet [3]histo= n...@lists.utsouthwestern.edu
I have a doctor who wants to stain some tissue with cd34. I need to
know if anyone can recommend a good positive control tissue for this
antibody. The staining will be done in paraffin embedded sections.
Thanks in advance.
Joel
--
Joel Reichensperger
Researcher II
Southern Illinois University
Plastic Surgery Institute
[4]jreichensper...@siumed.edu
217-545-7309 (Office)
217-545-1824 (Fax)
___
Histonet mailing list
[5]histo...@lists.utsouth= western.edu
[6]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet
References
1. 3Dhttp://www.pantomics.com/ 2. 3Dmailto:jreichensper...@siumed.edu;
3. 3Dmailto:histonet@lists.utsouthwestern.edu;
4. 3Dmailto:jreichensper...@siumed.edu;
5. 3Dmailto:Histonet@lists.utsouthwestern.edu;
6. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet;
___
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Histonet@lists.utsouthwestern.edu
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RE: [Histonet] CD34 positive control
Just about any tissue should work. It stains for Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts We use a sausage style control here with about 30 different types of tissue. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger [jreichensper...@siumed.edu] Sent: Wednesday, August 18, 2010 12:05 PM To: Histonet Subject: [Histonet] CD34 positive control I have a doctor who wants to stain some tissue with cd34. I need to know if anyone can recommend a good positive control tissue for this antibody. The staining will be done in paraffin embedded sections. Thanks in advance. Joel -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensper...@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Renaissance Containers
Hello , I need 4 good plastic containers, where you fill with xilene,alcool... for the VENTANA RENAISSANCE . Anyone could sell me ? Regards Ricky ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD34 Control
Joel, A section of skin will be a fine CD34 control. All collagenous connective tissue, including the dermis, are rich in CD34+ dendritic interstitial fibroblasts as well as CD34 positive endothelium in the resident vessels. Actually, in my lab we use a section of fallopian tube from tubal ligation, they are loaded with the fibroblasts and vessels. Jeff Silverman ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Frozen commercial controls for IgG, M and A for direct immunofluorescence?
Dear Histonet-ers, We currently use tonsil for a positive control tissue for IgG, A and M when we do direct immunofluorescence on skin specimens. We look for occasional plasma cells that stain positive in the cytoplasm, but of course there is no positive ID of what is staining, since the tonsil contains a mixture of plasma cells. Is anyone aware of a commercial source for a control for these reagents that can be used in DIF staining of frozen sections (not paraffin)? Many thanks, Luke luke.perko...@ucsf.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana Renaissance Containers
Hi Ricky, We may have what you need, we have multiple sizes of containers which we can either send empty or can fill for you whichever you prefer. Check out the site if you would like at www.sensorhealth.com Hope that helps. Adam Harris Sales Associate Sensor Health Inc. 110-6 Turnbull Crt. Cambridge, ON N1T 1K6 T: 1-888-777-7080 T: 519-621-1515 F: 519-621-8778 www.sensorhealth.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: CD34 positive control (McMahon, Loralee A) 2. Ventana Renaissance Containers (ricky hachy) 3. CD34 Control (Silverman, Jeffrey) -- Message: 1 Date: Wed, 18 Aug 2010 12:17:39 -0400 From: McMahon, Loralee A loralee_mcma...@urmc.rochester.edu Subject: RE: [Histonet] CD34 positive control To: Joel Reichensperger jreichensper...@siumed.edu, Histonet histonet@lists.utsouthwestern.edu Message-ID: c27aa2a01cef31469813089e226f582e02d5a7c...@urmcms7.urmc-sh.rochester.edu Content-Type: text/plain; charset=us-ascii Just about any tissue should work. It stains for Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts We use a sausage style control here with about 30 different types of tissue. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger [jreichensper...@siumed.edu] Sent: Wednesday, August 18, 2010 12:05 PM To: Histonet Subject: [Histonet] CD34 positive control I have a doctor who wants to stain some tissue with cd34. I need to know if anyone can recommend a good positive control tissue for this antibody. The staining will be done in paraffin embedded sections. Thanks in advance. Joel -- Joel Reichensperger Researcher II Southern Illinois University Plastic Surgery Institute jreichensper...@siumed.edu 217-545-7309 (Office) 217-545-1824 (Fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Wed, 18 Aug 2010 16:28:39 + From: ricky hachy elc...@hotmail.com Subject: [Histonet] Ventana Renaissance Containers To: Histonet histonet@lists.utsouthwestern.edu Message-ID: snt114-w54ab5f08e7e0cfc97193edcf...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 Hello , I need 4 good plastic containers, where you fill with xilene,alcool... for the VENTANA RENAISSANCE . Anyone could sell me ? Regards Ricky -- Message: 3 Date: Wed, 18 Aug 2010 12:30:58 -0400 From: Silverman, Jeffrey jsilver...@nshs.edu Subject: [Histonet] CD34 Control To: 'jreichensper...@siumed.edu' jreichensper...@siumed.edu Cc: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: 83f4d81747a7094dafe3ae87151ecb941ce4144...@sykechxvs01.nslijhs.net Content-Type: text/plain; charset=us-ascii Joel, A section of skin will be a fine CD34 control. All collagenous connective tissue, including the dermis, are rich in CD34+ dendritic interstitial fibroblasts as well as CD34 positive endothelium in the resident vessels. Actually, in my lab we use a section of fallopian tube from tubal ligation, they are loaded with the fibroblasts and vessels. Jeff Silverman -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 81, Issue 23 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Fontana
What stain platform are you using? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Wednesday, August 18, 2010 11:04 AM To: histonet Subject: [Histonet] Fontana Hello everone, We recently stained about 20 slides with Fontana for a research project. They were all fine except for 3 or 4 slides on which the pathologist says that the stratum corneum is overstained. The rest of the tissue is fine but that layer is too black. Now I have to fix the problem. Does anyone have an idea of why just that layer would overstain? On only a few slides of the set? Thank you! Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] I Have been scammed
Glad you're not stuck, Jerry. This is why I act like an old codger and refuse to get a facebook,other social networking sites, etc. account. What's the deal with farmville anyway? :) Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Jerry Santiago Sent: Tue 8/17/2010 10:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] I Have been scammed Histonetters, Thanks for all of your concerns and calls. I was made aware of the Europe scam and have contacted the authorities and they are doing what they suppose to do. I am still in Florida and have not left the country for any reason. My e-mail company states that this was stolen from facebook and gained access to my email account cleaning out all of my contacts and my access to my e-mail account as well as facebook. It will be fine and I apologize for anyone that got caught in this scam. Jerry Santiago Shands Jacksonville Jacksonville, FL 904-244-6149 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Advanced Stainers
I really have nothing bad to say about the benchmark. We love ours; it has freed us from having to nurse the IHC bench. You load it and walk away. If there was a down side I would say that the antibodies are a bit on the higher price side. You can find cheaper antibodies but then you have to use their dispensers and they are expensive as well. I guess it depends on what cost you put on tech time?? Is less technical time worth the higher cost?? For us it is a definite yes. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome alignment
Hi, My favorite tool works on all microtomes and is priced perfectly. The knobs on the block holder move the block so when it looks like you are going to cut too much of a block you turn them and, voila, a perfectly aligned microtome. Amos Message: 2 Date: Tue, 17 Aug 2010 14:16:26 -0500 From: Sharon.Davis-Devine sharon.davis-dev...@carle.com Subject: [Histonet] Microtome alignment To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: fe4513daaa85804d9a9fbac9c1ee7a668edbe...@exchangeccabe.carle.com Content-Type: text/plain; charset=us-ascii We are having a continuing issue of too much tissue being cut off when facing off a block for recuts. We have tried a couple of different methods for aligning our microtomes without much success. Does anyone out there have any advice on how to properly align them and what tool to use? Also, how often do you perform this re-alignment? The majority of our microtomes are older so more wear and tear and things move out of place more often. Any help or suggestions will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 81, Issue 18
Hi Emmanuel, I am a Canadian trained and certified tech working here in the US. You will have to contact the CSMLS (Canadian Society of Medical Laboratory Sciences) to find out were to send your educational info to apply for Canadian certification. You should be able to do a google search and get their website address. You must have Canadian certification to be able to work in any lab and there is probably no point in applying for a job in Canada until you have it. Joanne Clark, HT, MLT (Cdn) Histology Supervisor Pathology Consultants of New Mexico -- Message: 2 Date: Sat, 14 Aug 2010 17:37:24 -0500 From: Emmanuel O graceofgod011...@hotmail.com Subject: [Histonet] Seeking Position in Canada To: histonet@lists.utsouthwestern.edu Message-ID: col119-w5283faf640f630f71665c8de...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 I am a US trained and ASCP certified Histologist. I am looking for job opportunity in Canada either as Histologist or as an IHC Specialist. Thanking you all in advance. Emmanuel. -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 81, Issue 21
-- What we do is color code out microtomes (red, blue, green etc.) and each station has a matching marker. The tech will put a colored stripe down the side of all the blocks that he/she cut. If we have to pull a block out of the file for recuts, we know exactly which machine it was cut on by the color of the stripe on the side of the block. No re-alignment necessary. Joanne Clark, HT Histo Supervisor Path Consultants of NM Message: 2 Date: Tue, 17 Aug 2010 14:16:26 -0500 From: Sharon.Davis-Devine sharon.davis-dev...@carle.com Subject: [Histonet] Microtome alignment To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: fe4513daaa85804d9a9fbac9c1ee7a668edbe...@exchangeccabe.carle.com Content-Type: text/plain; charset=us-ascii We are having a continuing issue of too much tissue being cut off when facing off a block for recuts. We have tried a couple of different methods for aligning our microtomes without much success. Does anyone out there have any advice on how to properly align them and what tool to use? Also, how often do you perform this re-alignment? The majority of our microtomes are older so more wear and tear and things move out of place more often. Any help or suggestions will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE CD34 CONTROL
hI, Placenta is a very good control for CD34,try it you will like it. Tunde ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
