[Histonet] special stain for H. billis
Good morning everyone, Does anyone know of a special stain that is specifically for H. billis. I don't know much about bacteria, so I am not even sure which bacterial stain would work on this. Any comments would be appreciated. Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 81, Issue 33
-Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, August 25, 2010 12:33 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 81, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. I will be out of office beginning the afternoon of 8/23 and returning 8/31 4 2010 and returning 8/13/2010 (marilyn.a.we...@kp.org) 2. preparation of frozen sections (Tench, Bill) 3. Re: Alcian Yellow (Robert Richmond) 4. PMS2 (dianar...@aol.com) 5. Technovit 9100 New (C B) 6. RE: preparation of frozen sections (gayle callis) 7. Re: Technovit 9100 New (Jack Ratliff) 8. Re: Technovit 9100 New (Jack Ratliff) 9. Re: shrinkage (louise renton) 10. Re: PMS2 (Dana Settembre) 11. porcine CD31 FFPE (C B) 12. Lectin From Arachis hypogaea(peanut)- peroxidase Staining (Chakib Boussahmain) 13. RE: Ventana vs Leica (Houston, Ronald) 14. shrinkage/a howlong is a piece of string type question (Edwards, Richard E.) 15. RE: Ventana vs Leica (Maria Katleba) 16. Testing for shrinkage RE: [Histonet] shrinkage/a howlong is a piece of string type question (gayle callis) 17. Re: Testing for shrinkage RE: [Histonet] shrinkage/a howlong is a piece of string type question (jan.mins...@leica-microsystems.com) -- Message: 1 Date: Tue, 24 Aug 2010 16:02:43 -0700 From: marilyn.a.we...@kp.org Subject: [Histonet] I will be out of office beginning the afternoon of 8/23 and returning 8/31 4 2010 and returning 8/13/2010 To: histonet@lists.utsouthwestern.edu Message-ID: offa37bc14.1bca8f4f-on88257789.007e97a5-88257789.007e9...@kp.org Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 08/23/2010 and will not return until 08/31/2010. In my absence please ask for Mary Campbell . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. -- Message: 2 Date: Tue, 24 Aug 2010 16:07:09 -0700 From: Tench, Bill bill.te...@pph.org Subject: [Histonet] preparation of frozen sections To: Histonet@lists.utsouthwestern.edu Message-ID: 2820431bf953bb4da3e9e1a5882265fd034a5...@mail1.pph.local Content-Type: text/plain; charset=us-ascii So as a pathologist, i have to ask you why you would want to air dry a section? From a diagnostic perspective, we consider air dried samples unacceptable in my lab. All of our standard histologic interpretation is based on fixed sections. So, why not drop the slide in a jar or ETOH and keep it there until you are ready to stain? Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - -- Message: 3 Date: Tue, 24 Aug 2010 20:31:40 -0400 From: Robert Richmond rsrichm...@gmail.com Subject: [Histonet] Re: Alcian Yellow To: histonet@lists.utsouthwestern.edu Message-ID: aanlktikms4on2o+w=-qskabd=uza9jgskdkibit2v...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Jennifer Johnson asks: Can I get someone to share their source for Alcian Yellow? Our Pathologist wants to try a different method for Helicobacter and I need powdered AY? I'd suggest you look into Anatech's method, which bypasses Alcian yellow. (I have no connection with Anatech.) Bob Richmond Samurai Pathologist Knoxville TN -- Message: 4 Date: Tue, 24 Aug 2010 20:47:31 EDT From: dianar...@aol.com Subject: [Histonet] PMS2 To: histonet@lists.utsouthwestern.edu Message-ID:
RE: [Histonet] Ventana vs Leica
We love our Ventana instruments too Jay. I don't quite believe the 40% difference in cost. I'd like to see those numbers. I know I save in tech time and the instruments are very easy to use. We have histo assistants and secretaries trained to load and unload the instruments, saving out techs to do the things only techs can do. Talk about LEAN! A little more from a LEAN perspective, the Ultra is the only instrument out there that is TRUE continuous flow. As soon as there is an open spot on the instrument and the antibody is on board, I can add a slide. I don't have to wait till one of the 10 slide modules is finished. Leica is still a batch instrument, it is just smaller batches than the older IHC models. I'm not putting Leica down, it is a fine instrument but I think it is important for people to know the facts. Jan Mahoney Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of bsulli...@shorememorial.org Sent: Tuesday, August 24, 2010 2:05 PM To: Jay Lundgren Cc: histonet; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Jay Lundgren jaylundg...@gmai l.com To Sent by: histonet histonet-bounces@ histonet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu Subject [Histonet] Ventana vs Leica 08/24/2010 02:30 PM I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. If this is you, could you please tell me which you preferred and why. I'm currently working for a facility in MT which has narrowed down its search to these two instruments. No vendors please, they've already given their pitches. Thanks, Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cleaning molds
Does anyone in histoland clean their embedding molds in a dishwasher? Otherwise, besides placing in the cleaning cycle of your processor, how do sites clean their molds?? Simple, but plaguing question!!! Thanks all! Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cleaning molds
We clean our stainless steel base molds by soaking in solvent (i.e. citrisolv, etc.) then wash, by hand, with soap and water. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Thursday, August 26, 2010 11:54 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cleaning molds Does anyone in histoland clean their embedding molds in a dishwasher? Otherwise, besides placing in the cleaning cycle of your processor, how do sites clean their molds?? Simple, but plaguing question!!! Thanks all! Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cleaning molds
Back when I was using metal cassette lids and an autotechnicon (so no cleaning in the processor) I would throw all the lids and molds into a pot after embedding. Throw a bit of laboratory detergent in, fill with water, and boil on a hot plate. Once I got to a rolling boil, I would turn the heat off. By the end of the day it was cool. Peel the paraffin off the top, then rinse the molds. Worked great! Didn't even need mold release. Will Sent from my Verizon Wireless BlackBerry -Original Message- From: Webb, Dorothy L dorothy.l.w...@healthpartners.com Sender: histonet-boun...@lists.utsouthwestern.edu Date: Thu, 26 Aug 2010 10:53:58 To: 'histonet@lists.utsouthwestern.edu'histonet@lists.utsouthwestern.edu Subject: [Histonet] Cleaning molds Does anyone in histoland clean their embedding molds in a dishwasher? Otherwise, besides placing in the cleaning cycle of your processor, how do sites clean their molds?? Simple, but plaguing question!!! Thanks all! Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cleaning molds
The best way to clean the molds is to boil them in a 2% aq. sol. of dishwasher detergent. Now, this is an extra step so if you clean the tissue processor anyway, it is one chose less to just place them in the cleaning cycle.René J. --- On Thu, 8/26/10, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: From: Webb, Dorothy L dorothy.l.w...@healthpartners.com Subject: [Histonet] Cleaning molds To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Date: Thursday, August 26, 2010, 11:53 AM Does anyone in histoland clean their embedding molds in a dishwasher? Otherwise, besides placing in the cleaning cycle of your processor, how do sites clean their molds?? Simple, but plaguing question!!! Thanks all! Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Cleaning molds
We use an ultrasonic bath filled with water with a bit of dishwasher. Temperature about 40 allows the paraffin to get off the molds and to swim on the surface. Needs 15 min. Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Webb, Dorothy L Gesendet: Donnerstag, 26. August 2010 17:54 An: 'histonet@lists.utsouthwestern.edu' Betreff: [Histonet] Cleaning molds Does anyone in histoland clean their embedding molds in a dishwasher? Otherwise, besides placing in the cleaning cycle of your processor, how do sites clean their molds?? Simple, but plaguing question!!! Thanks all! Dorothy Webb This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ventana HPv
Does anyone use the Ventana to process HPV for tissue and paps? Barbara Crill DRMC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rapid liver core biopsy processing
Dear histonetters, We do about a hundred liver transplants/year at our hospital, and the hepatologists do lots of liver core biopsies to rule out rejection. They want a same-day diagnosis on these, so historically they have been rush processed on a two-hour cycle (VIP machine). They are brought over directly from the liver biopsy suite immediately after biopsy, so they get very little fixation in the specimen container prior to going on the processor. Recently we had a couple of sub-par cases and have moved to a four-hour processing cycle. Do any of you have any experience dealing with rush-processed liver cores in transplant patients? What sort of a processing schedule do you recommend? Anyone handling them like frozen sections? Thanks, Garth Fraga (pathologist) University of Kansas Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome Repair - New England Area?
Any recommendations for microtome repairs (preferably on-site), preferably in the MA-RI-CT area? Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Rapid liver core biopsy processing
We handle them when we get them as frozen sections. The Pathologist are able to give a rapid diagnosis but they do prefer the formalin fixed tissue. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Garth Fraga Sent: Thursday, August 26, 2010 1:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rapid liver core biopsy processing Dear histonetters, We do about a hundred liver transplants/year at our hospital, and the hepatologists do lots of liver core biopsies to rule out rejection. They want a same-day diagnosis on these, so historically they have been rush processed on a two-hour cycle (VIP machine). They are brought over directly from the liver biopsy suite immediately after biopsy, so they get very little fixation in the specimen container prior to going on the processor. Recently we had a couple of sub-par cases and have moved to a four-hour processing cycle. Do any of you have any experience dealing with rush-processed liver cores in transplant patients? What sort of a processing schedule do you recommend? Anyone handling them like frozen sections? Thanks, Garth Fraga (pathologist) University of Kansas Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Position In Alabama
FINALLY another HistoTech position has been approved at DCH Regional Medical Center in Tuscaloosa, Alabama. We are a not for profit facility in West Alabama about 50 miles from Birmingham, about 4 hours from the Gulf of Mexico, and about 3 hours from Atlanta. We process about 15000 surgicals per year using Sakura VIP conventional processor, and Sakura ExPress 50 Rapid Tissue Processor, Sakura Prisma HE Stainer with tape coverslipper and Ventana IHC automation. Hopefully we will be automated in Special Stains after October 1. Interested candidates must be proficient in embedding, microtomy, frozen sections, and (for now) manual special staining. Please contact Michelle Fagin at 205-759-7762 or Fax 205-750-5224 OR Sherrie Faulkner at 205-750-5736 or email mfa...@dchsystem.com Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Lung biopsies - Question
We cut 10 unstained slides. Rae Staskiewicz Methodist Medical Center of Illinois -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, August 26, 2010 3:54 PM To: Histonet Subject: [Histonet] Lung biopsies - Question How many labs cut extra tissue sections (or tissue curls) for molecular testing (KRAS, BRAF, EGFR, etc.) on lung biopsies up front (at the time of HE sectioning)? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana vs Leica
Unless someone corrects me (or even agrees with me!) in FL only a technologist is allowed to load the IHC machine, so no additional lean for us. :o( I would be interested to hear more about the savings though, as we are preparing to be in the market for a new IHC machine. We currently have the Benchmark XT. On Aug 26, 2010, at 10:08 AM, Mahoney,Janice A janice.maho...@alegent.org wrote: We love our Ventana instruments too Jay. I don't quite believe the 40% difference in cost. I'd like to see those numbers. I know I save in tech time and the instruments are very easy to use. We have histo assistants and secretaries trained to load and unload the instruments, saving out techs to do the things only techs can do. Talk about LEAN! A little more from a LEAN perspective, the Ultra is the only instrument out there that is TRUE continuous flow. As soon as there is an open spot on the instrument and the antibody is on board, I can add a slide. I don't have to wait till one of the 10 slide modules is finished. Leica is still a batch instrument, it is just smaller batches than the older IHC models. I'm not putting Leica down, it is a fine instrument but I think it is important for people to know the facts. Jan Mahoney Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of bsulli...@shorememorial.org Sent: Tuesday, August 24, 2010 2:05 PM To: Jay Lundgren Cc: histonet; histonet-boun...@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana vs Leica Jay, I currently use the Ventana and am very pleased with the results I get. The only draw back is the cost to run the instrument. It can get quite pricey. They added space on the antibody wheel but took space away from the slide area. This has impacted our work flow greatly. We are however looking to purchase a second one. This one will have continual through put. That should help out with TAT. Hope this helps. Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.) AP Supervisor Shore Memorial Hospital 609-653-3590 Jay Lundgren jaylundg...@gmai l.com To Sent by: histonet histonet-bounces@ histonet@lists.utsouthwestern.edu lists.utsouthwest cc ern.edu Subject [Histonet] Ventana vs Leica 08/24/2010 02:30 PM I was wondering if anyone out there had experience with both the Ventana Ultra and the Leica Bond immunostainers. I realize that most people have a personal preference as to brands, but I'm not looking for a knee-jerk opinion (LEICA RULZ11 or VENTANA FTW!!), just someone who has had actual experience working on a daily basis with both instruments. If this is you, could you please tell me which you preferred and why. I'm currently working for a facility in MT which has narrowed down its search to these two instruments. No vendors please, they've already given their pitches. Thanks, Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list
[Histonet] Cleaning molds
We throw ours in a pot with a little soap in the bottom and boil, unplug and let the paraffin harden. Just take the paraffin disk off and rinse the molds in cold water and lay out to dry. No reason to gunk up the expensive tissue processor. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ventana HPv
We used to do pap HPV's on our Ventana. Whatcha need? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cytokeratin 18 in liver
Hello all. Just wondering if anyone has experience with cytokeratin 18 immunohistochemistry labelling in liver (FFPE)? I have used an Abcam rabbit anti human CK18 to try and label hepatocytes in both human and mouse adult liver and have found that I get strong labelling in bile duct epithelia but essentially nothing in hepatocytes. I say essentially nothing, but there does appear to be faint possible specific staining in a few hepatocytes. From my reading, I had thought that cytokeratin 18 (and 8) are abundantly expressed in hepatocytes and should be easy to label, but that is not what I have found. We are trying to tissue engineer liver from progenitor cells in a mouse model, and need to be able to identify these cells, whether liver progenitors or mature / differentiated hepatocytes, hence my choice of an antibody against CK18 which should label both. We have also used a pan cytokeratin (Dako Z0622) with mixed results and were hoping for something a little more defined. Any thoughts greatly appreciated, Jason Jason Palmer Histology Laboratory Coordinator O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4045 fax +61 3 9416 0926 email: jason.pal...@svhm.org.au Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. __ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
