[Histonet] histomorphometry - clarified
Dear all, just to clarify issues here - i have a histomorphometry analysis system -a very nice one, and i sort of know how to use it - what i DON'T know is what sort of measurements i should be doing. So thanks to all who suggested systems, i'm sorry if my initial e-mail was not all that clear. further commentary on WHAT to measure and what parametres are useful will be welcomed best regards On Fri, Oct 1, 2010 at 12:12 PM, Katie McKinley katie.mckin...@i-path.co.uk wrote: Dear Louise, We have software which not only will allow the easy measurement of slides but also the management of your images. We specialize in whole slide scanning software, We can scan your slides and your images are saved on our network of servers and can be accessed online using secure login details. Because the software is online you can access your images from any location. The scan can be treated like a glass slide. You can zoom in to diagnostic resolution and pan around the whole slide and move through the layers. As well as measurements, you can annotate the slide, build up archives and banks of information and share with colleagues around the world instantly. We are currently in beta testing of our off the shelf imaging analysis software, but we do work on a consultancy basis to create analysis algorithms to suit our clients' needs specifically. Please visit our website for more info. www.i-path.co.uk Kind Regards Katie -Original Message- From: Wagner, Richard [BUL/LAK] [mailto:richard.wag...@buehler.com] Sent: 28 September 2010 21:48 To: louise.ren...@gmail.com Cc: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] histomorphometry Louise: Versatile image analysis software like OmniMet can solve this problem. Powerful digital image acquisition, object and field measurement options, and counting tools are standard features of the software. http://www.buehler.com/productinfo/biomedical/ia.htm Regards, Rick Wagner -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff Sent: Tuesday, September 28, 2010 8:36 AM To: louise renton Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] histomorphometry At the NSH this year, just this last Sunday in fact, Dr. Tony Villanueva demonstrated this very technique in his workshop. My best advice would be to contact him directly (avillanuev...@cox.net) and see what he can do for you. Another option would be to contact an image analysis vendor like BIOQUANT (nathan...@bioquant.com). Hope this information helps! Regards, Jack On Sep 28, 2010, at 3:25 AM, louise renton louise.ren...@gmail.com wrote: Hi all, I desperately need some advice from an experienced histomorphometristI am trying to translate the old cumbersome visual measurement of using an eypiece grid and doing point counting to an easier computer system - but I am not sure how to do it - any help out there? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Marking inks
What are labs using for marking inks for surgical pathology specimens? I have been told that we are having problems with the product that we are currently using. Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PCR for Yersinia
Anyone doing PCR for Yersinia on formalin-fixed, paraffin-embedded tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] gliotic plaques etc
Hello, Not to be a nag, but I am still looking forward to some comments regarding the below: Message: 8 Date: Tue, 28 Sep 2010 09:43:25 +0200 From: Tyrone Genade tgen...@gmail.com Subject: [Histonet] gliotic plaques etc I'm interested in quantifying gliotic plaques, tangles and amyloid plaques in 5 um thick wax sections (fixed in Bouins). I'm planning on using Toluidine Blue for the amyloid and then Gallays Silver or Thioflavine S for the tangles. Any comments or suggestions? Any one have any experience with these stains for this purpose? The gliotic plaques are more confounding. I have seen a slide where HE was used and the eosin stained the plaques bright pink-red. Is this typical? Otherwise, I was thinking about PTAH. Anyone have a favourite method for this purpose? I have about 400 slides to go through so an IHC method is out, being to expensive. I have come across the paper by Manlow et al (J. Neuropath. Expr Neurology, 51:298-302, 1992) for a modified PTAH stain. Anyone used their method? Its not mentioned in Theory and practice of histological techniques. Thanks -- Tyrone Genade http://tgenade.freeshell.org email: tgen...@gmail.com tel: +27-84-632-1925 (c) Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Recall: [Histonet] FW: DAB
Morken, Tim would like to recall the message, [Histonet] FW: DAB. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Marking inks
We use marking ink from Statlab or Mercedes Medical. After applying the ink to the tissue, we take weak acetic acid solution and blot it over the ink which helps it adhere. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 01, 2010 9:41 AM To: Histonet Subject: [Histonet] Marking inks What are labs using for marking inks for surgical pathology specimens? I have been told that we are having problems with the product that we are currently using. Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Marking inks
I use inks from 3 different companies just because I need the variety of colors offered from each one. Fisher, Cardinal and a company in California. I have found that if I ink a specimen and dip the specimen in a solution of 10% acetic acid for a couple of seconds all ink shows up after processing wonderfully. I used to use straight acetone to dip in but found that you must leave the specimen for 10 seconds or longer for it to stay on after processing. One of these techniques might solve the problem. Good luck -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 01, 2010 9:41 AM To: Histonet Subject: [Histonet] Marking inks What are labs using for marking inks for surgical pathology specimens? I have been told that we are having problems with the product that we are currently using. Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice of Legal Status and Confidential Information: This electronic mail message and any accompanying attachments may contain information that is privileged and CONFIDENTIAL. If you are not the intended recipient you are advised that any use, review, dissemination, distribution or reproduction of the information is strictly prohibited and may be unlawful. If you have received this communication in error, please notify the sender immediately and destroy the transmitted information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Marking inks
We use Davidson inks, except for the green. The green was gumming up our VIP processors. In fact we found out that Dr. Davidson does not recommend using the green for tissues to go through a processor, but that was not in the datasheet. So we use Cancer Research for the green ink. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hayden, Rebecca Sent: Friday, October 01, 2010 8:57 AM To: 'Richard Cartun'; Histonet Subject: RE: [Histonet] Marking inks I use inks from 3 different companies just because I need the variety of colors offered from each one. Fisher, Cardinal and a company in California. I have found that if I ink a specimen and dip the specimen in a solution of 10% acetic acid for a couple of seconds all ink shows up after processing wonderfully. I used to use straight acetone to dip in but found that you must leave the specimen for 10 seconds or longer for it to stay on after processing. One of these techniques might solve the problem. Good luck -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 01, 2010 9:41 AM To: Histonet Subject: [Histonet] Marking inks What are labs using for marking inks for surgical pathology specimens? I have been told that we are having problems with the product that we are currently using. Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice of Legal Status and Confidential Information: This electronic mail message and any accompanying attachments may contain information that is privileged and CONFIDENTIAL. If you are not the intended recipient you are advised that any use, review, dissemination, distribution or reproduction of the information is strictly prohibited and may be unlawful. If you have received this communication in error, please notify the sender immediately and destroy the transmitted information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Marking inks
We use Statlab and Fisher marking inks. We dip our specimens in Bouin's or Vinegar to set the ink. We don't have any problems with the ink coming off. Hope that helps. Thanks, Timothy Higgins, HT(ASCP) QIHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton Sent: Friday, October 01, 2010 10:54 AM To: 'Richard Cartun'; Histonet Subject: RE: [Histonet] Marking inks We use marking ink from Statlab or Mercedes Medical. After applying the ink to the tissue, we take weak acetic acid solution and blot it over the ink which helps it adhere. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 01, 2010 9:41 AM To: Histonet Subject: [Histonet] Marking inks What are labs using for marking inks for surgical pathology specimens? I have been told that we are having problems with the product that we are currently using. Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Seeking accession stickers - rainbow 1x10
Hello everyone! Help!! We're about to run out of our usual accessioning label stickers. These are single lines of 10 stickers on a pin-fed continuous form dot matrix sheet. Each line of labels is one color rotating through 5 colors, so we have a numbering program that prints each line with one accession number, giving us 10 separate single-color stickers to put on our requisition, specimen bottle and cap. The colors help us keep things from getting mixed up but our usual source is no longer available. Does anyone know of a source or an alternate way to produce labels to achieve the same end? Cheryl Kerry, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PA hourly wage
What is the average hourly wage for a PA that grosses approx. 5000 cases a year. __ Information from ESET NOD32 Antivirus, version of virus signature database 5495 (20101001) __ The message was checked by ESET NOD32 Antivirus. http://www.eset.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Correlation studies
Posting for a friend. Please replay to me. If you have switched from Ventana Benchmark to Ventana Ultra. Could you please let us know what kind of correlation studies you did between machines.? Thank you very much. Susan Baker Saint Joseph Histology lab Supervisor Bryan, Texas. Lin Bustamante Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] change in immuno machines
If you changed machines, you need to validate the staining on that machine just as if you never had the first machine. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Marking inks
Richard Cartun asks about marking inks used for surgical pathology specimens. if I just need one color (great majority of specimens) I use india ink bought from a stationer or craft supply store - cheap, easily replaced, and it gets the job done. If I need colors, I prefer the Davidson marking inks, available through some though not all vendors. They offer seven colors, including orange (they have to sell their product in Tennessee and Texas, after all!) and purple. (I have no commercial connection with Davidson inks.) I blot my specimens thoroughly dry, and don't use a fixative. If I did, I'd use white vinegar (5% acetic acid), possibly diluted 1:1 with water. Bouin's fixative and acetone both have obvious environmental problems. Some people use tattoo inks. Cheap and available in a huge number of colors, but you have to read some truly disgusting catalogs to get them. I didn't know about green Davidson inks gumming up processors - thanks for that tip. (I wonder why other inks should be different.) With all marking inks, it's very important to re-cap the bottle as soon as you're through with it. If you re-cap promptly, an opened bottle will keep for years. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Job Opening on Long Island
Allied Search Partners has been retained to prescreen and search for a qualified Histotechnician or Histotechnologist candidate in New York, NY. Location: On Long Island, near Hicksville, NY Shift: - Full Time/Permanent (Long Term) - Day Shift 8am-5pm, Monday-Friday Environment: - A premier medical practice with several locations throughout NY and NJ consisting of about 24 doctors. - Private Laboratory and Team Oriented/Family Oriented Environment - No Quotas! Have the flexibility to get your work done on your own time and no weekends - State of the art equipment Requirements: - Ability to work with little or no supervision - NY licensed - ASCP certified - Meet the CLIA eligibility to gross Benefits Pay: Salary is dependent upon experience and is competitive to current market rate Benefits can be discussed with Hiring Manager during a phone screen To apply: Please first submit resume to aly...@alliedsearchpartners.com . At that time we will review your resume with our team of recruiters and if all requirements are met will schedule a phone screen with you to discuss further questions and concerns. * * -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] change in immuno machines
Agreed. Thanks, Timothy Higgins, HT(ASCP) QIHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tench, Bill Sent: Friday, October 01, 2010 12:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] change in immuno machines If you changed machines, you need to validate the staining on that machine just as if you never had the first machine. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dako Autostainer Plus for sale
Hello Everyone, We have several pieces of equipment for sale, including our Dako Autostainer Plus (3800 Series) staining system. Please check us out at www.sarapath.com/equipment. Thanks, Brian Branton Purchasing Agent SaraPath Diagnostics Sarasota Pathology Sarasota Professional Enterprises II (941) 362-8963 (941) 362-8964 FAX ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] PA hourly wage
Usually the hourly wage has nothing to do with the workload. That is something the PA has to negotiate with the lab manager. The work load will determine the number of PAs hired, if any. René J. --- On Fri, 10/1/10, r.c.st...@comcast.net r.c.st...@comcast.net wrote: From: r.c.st...@comcast.net r.c.st...@comcast.net Subject: [Histonet] PA hourly wage To: histonet@lists.utsouthwestern.edu Date: Friday, October 1, 2010, 12:40 PM What is the average hourly wage for a PA that grosses approx. 5000 cases a year. __ Information from ESET NOD32 Antivirus, version of virus signature database 5495 (20101001) __ The message was checked by ESET NOD32 Antivirus. http://www.eset.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] The Fifth Annual International Retreat on Applied IHC and Molecular Pathology
I am forwarding this announcement on behalf of Dr. Hadi Yaziji: Dear colleagues, We just finalized the schedule for our fifth annual course. Many of you have already attended our previous courses. Feel free to forward this email to any interested colleagues. Also, exhibitors are welcome for sponsorship opportunities. We have a limited room this year, based on first come-first serve basis. All information about schedule, location, time, faculty, exhibitors prospectus, contact info, registration form, are available and downloadable online from the course's website at www.pathlearning.com Thanks, Hadi Hadi Yaziji, MD I Medical Director Vitro Molecular Laboratories I www.vitromolecular.com 7480 SW 40th Street, Suite 700 Miami, FL 33155 Tel 305 267 7979 I Fax 786 513 0175 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HMGB and RAGE; TLR2, TLR4 antibodies
Hi, I am currently working on HMGB1. It is a weird one! If you go by the data sheets you will get great nuclear staining on all the nuclei. This is not what you are after if you have necrotic tissue. What you want to see is the nuclei in necrotic tissue fade out and have the cytoplasm take it up. I am cutting the dilution to 1: 50 and 1:100 since we were just starting to see blushes of cytoplasmic staining and no nuclear label at higher dilutions. Usually you get staining that fades out at higher dilutions. This one was really odd. Good luck, Amos Message: 13 Date: Thu, 30 Sep 2010 04:55:02 +0200 From: Fabrice GANKAM gan...@googlemail.com Subject: [Histonet] HMGB and RAGE; TLR2, TLR4 antibodies To: histonet@lists.utsouthwestern.edu Message-ID: c4a46c631cd14a86aad3ce3abb29a...@pcdegankam Content-Type: text/plain; charset=us-ascii Hey guys Just wanted to know if any of you had some luck with an antibody aigainst rat HMGB1 and its receptors RAGE, TLR2, TLR4. W Thanks Dr Fabrice GANKAM UTSW ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
