[Histonet] pyrosequencing
Dear Histonetters! Is anyone with experiences on pyrosequencing kras out there? I would be happy to have someone I could share my problems with. ;-) Gudrun Lang Histolab Linz, Austria ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cryojane problems
Brett- I have been using the 4X slides, but they don't seem to help. I dont have this problem with the cartilage alone, but when there is bone attached, it is a nightmare. Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Connolly, Brett M brett_conno...@merck.com To: Kim Merriam kmerriam2...@yahoo.com; Histonet histonet@lists.utsouthwestern.edu Sent: Tue, October 26, 2010 9:41:18 AM Subject: RE: [Histonet] cryojane problems Kim, I would recommend using their '4x' slides - they have more adhesive on them. I don't think flashing more than once has any benefit. Brett Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Tuesday, October 26, 2010 9:32 AM To: Histonet Subject: [Histonet] cryojane problems Hi All, I have been using the cryojane to cut human articular cartilage, with excellent results. Now I need to cut frozen sections of rat articular cartilage that contain some underlying bone. I am getting nice sections, but when I peel the tape off the slide, a bunch of my tissue is staying on the tape. I have tried increasing the number of flashes (to a whopping 10!), hoping that this will help adhere the sections, but it hasn't. Any tips for me? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Be recognized for making a difference! Anatomic Pathology Manager position
Good Morning! Slone Partners seeks an Anatomic Pathology Manager for a fast-paced laboratory in a nationally recognized teaching hospital in the Chicago area. Three to five years of supervisory or management experience is required. The ideal candidate will have histology management experience in an academic medical center environment. Special features of this position. The hospital is looking for a strong leader who wants to be recognized for the difference he or she can make. It offers excellent benefits and a stable work environment, and encourages innovation, embraces diversity, and values human dignity. If you meet these qualifications and would like to be considered for this opportunity, please contact Therese Cook at there...@slonepartners.com. Best Regards, Therese Cook MT (ASCP) SLONEPARTNERS THERESE COOK - EXECUTIVE RECRUITER Corporate Headquarters 1521 Alton Road #638 Miami Beach, Florida 33139 TOLL FREE: 877.419.1439 DIRECT: 330.863.1054 CELL: 330.323.4525 FAX:330.232.9333 www.slonepartners.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Good day, Our private dermpath lab, located in central/western NY is eager to fill an immediate opening for a full time histotech. A relocation bonus is negotiable. To learn more please contact me directly. Jen Campbell Muhlbauer Dermatopathology Laboratory Supervisor of Technical Services Phone 585-586-5166 Fax 585-586-3137 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thanks interfacing the IHC bond and Cassettelabelers to Co-Path
Hi Histonet Land, I just wanted to drop you a line and thank everyone that sent me direct and indirect information regarding my question interfacing the IHC bond and Cassettelabelers to Co-Path I forwarded the information to the administrator that had proposed the question. She was able to prose her information to the proper channels to make her case for justification. Thank you again! Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: xylene substitute
xylene substitutes also require more time than xylene Johnson, Teri t...@stowers.org Sent by: histonet-boun...@lists.utsouthwestern.edu 10/27/2010 10:13 AM To 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu cc Subject [Histonet] Re: xylene substitute Hi all, I just wanted to let folks know that if you plan to switch to a xylene substitute, realize that the substitutes are more intolerant of water than xylene is. Your last alcohol will have to be as anhydrous as you can get it or you will have water carryover that may cause some problems. If you live in a humid climate, this might end up being problematic for you. Having said that, we prefer using ProPar or Clear-Rite 3 (aliphatic hydrocarbon reagents) over the limonenes. Carry on! Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Absolute with methanol
Does anyone knows if there is any problem using Absolute ETOH with Methanol instead Absolute alone when it comes to make any staining solutions? Thank you. Lin. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Parafolicular cells, C cells
Do you know a good specific special stain (not IHC stain) for this cells of the thyroid gland? Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GMA - 100 um sections
Anyone have done this? Please let us know what you did. Thank you. Lin. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Absolute with methanol
It should not be a problem as most reagent alcohol contains both methanol and isopropanol at roughly 90% ETOH with 5% of each of the others. This way the alcohol is not taxed as it is unfit for human consumption according to the ATF. The goal was to make an alcohol we could afford to buy in the for profit sector that met the non-consumption rule, since it is poison, so to speak. Pam Marcum UAMS - Original Message - From: Lin Bustamante lbustama...@cvm.tamu.edu To: histonet@lists.utsouthwestern.edu Sent: Wednesday, October 27, 2010 3:19:36 PM Subject: [Histonet] Absolute with methanol Does anyone knows if there is any problem using Absolute ETOH with Methanol instead Absolute alone when it comes to make any staining solutions? Thank you. Lin. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Original Message - From: Lin Bustamante lbustama...@cvm.tamu.edu To: histonet@lists.utsouthwestern.edu Sent: Wednesday, October 27, 2010 3:19:36 PM Subject: [Histonet] Absolute with methanol Does anyone knows if there is any problem using Absolute ETOH with Methanol instead Absolute alone when it comes to make any staining solutions? Thank you. Lin. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] lever vs handwheel?
Can someone tell me the difference between a handwheel driven cryostat and a 'lever' driven cryostat? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
