[Histonet] RELIA Special Job Alert for Managers and Supervisors. 11-09-10
Hello Histonetters! I have several exciting opportunities for experienced Managers, and Supervisors in hospital and private lab environments in several locations nationwide. These are some of the premier employers in the United States. The positions are of course full time and permanent. My clients offer excellent compensation, benefits and relocation assistance. Here are my Management Opportunities: Histology Supervisor Colorado Springs, CO Core Lab Director Little Rock, AR Histology Manager Modesto, CA QA Manager Orange/Rockland County, NY If you would like more information or know of someone else who might be interested, please contact me at rel...@earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam RELIA is offering a $500 referral bonus for anyone you refer and I place and a $500 hiring bonus if I place you! There are a lot of recruiters out there right now trying to work with histology professionals and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 8 years I have dedicated my practice solely to placing histology professionals like you. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] looking for references to stain protocols
I am in need of references for stain protocols. The stain I am searching for in particular is the modified Gimenez, also known as Pierce-Vanderkamp for chlamydia and rickettsia. If anyone can point me in the right direction I would appreciate it. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: looking for references to stain protocols
This is the reference that I have for this stain. Andersen A.A. VanRompay D (2005). Chlamydiosis. In: A Laboratory Manual for the Isolation and Identification of Avian Pathogens, Fifth Edition. Submitted USA. Roberta Horner HT/HTL Animal Diagnostic La Penn State University -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Tuesday, November 09, 2010 12:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] looking for references to stain protocols I am in need of references for stain protocols. The stain I am searching for in particular is the modified Gimenez, also known as Pierce-Vanderkamp for chlamydia and rickettsia. If anyone can point me in the right direction I would appreciate it. Lynn Burton Lab Assoc. I Animal Disease Lab Galesburg, Il 61401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Jaclyn Bhamornsiri - Important Message
I will be out of the office starting 11/09/2010 and will not return until 12/31/2014. As of Nov 9th, 2010, I am no longer with VWR. If you need assistance, pls contact VWR Customer Service at 1800-932-5000 (or vwrcustomerserv...@vwr.com). For more urgent matters, pls contact Leize Lessig at 770-733-8190 (or leize_les...@vwr.com). Thanks! Jackie B. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Lab Survey
Dear Histonetters, Scientific Services at The Jackson Laboratory is conducting benchmarking surveys in an effort to develop an understanding of the current best practices in operations, management and technical delivery at peer institutions. The goal is to gather quality data that can be used to assist us and all survey participants with operational assessment and planning. Survey participants will receive compiled results which will be de-identified before distribution. If you would like to participate, just click on the link below and answer the questions in the survey. Additionally, for those of you who do EM, there is a link to an EM Facility survey as well. It will only take a few minutes of your time. With thanks in advance! Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) Histo Survey - http://www.surveymonkey.com/s/5RPSYG2 EM Survey - http://www.surveymonkey.com/s/5RGYH9W ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cutting and embedding
Could you please tell me what is the average for embedding surgical blocks in one hour? Is there an average for cutting? Thank you. Lin. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NSH Validation WS attendees
For those of you who attended the IHC ws at NSH on Validation by Tim Morken
and Patsy Ruegg we have posted the rest of the information we promised you
to my ftp://ihctech.net ftp://ihctech.net/ site for you to download. We
provided the user name and pass word you will need in the workshop. The
folder is labeled Tim Morken and you can download the files to your
computer. Remember this site is for uploading and downloading not opening.
Best regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email mailto:pru...@ihctech.net pru...@ihctech.net
web site http://www.ihctech.net www.ihctech.net
This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
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any dissemination, distribution, copying, or other use of this message, or
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[Histonet] how to fix floating sections?
Hello Everyone, I have a bunch of rat brains that I have frozen in isopentane and stored at –80. I'd like to collect 300 micron sections on a cryostat and collect tissue punches for RNA isolation. I would then like to take the remaining tissue and stain. There are a few ways of doing this, what would work the best is to take two 50 micron section, followed by a 300 micron, followed by two more 50, and go through the brain serially. I will then stain the 50 micron sections with nissl or thionin, so that the structures are easily identified and then use these to direct the tissue punches. The second 50 micron sections would be reserved for GFP IHC. Is this feasible? Here are some direct questions: 1. how would I store the 300 micron sections? 2. Any suggestions/protocols for staining the 50 micron sections for structure? Which stain would you use? 3. Can I post-fix the 50 micron sections? How about the 300 micron? One reason I want to use floating sections for the nissl/thionin is that I can mount these very flat, no wrinkles and I'm very comfortable working with floating sections. I don't want to risk precious tissue trying to optimize this. One methodology recommended to me is to freeze a formalin solution in a 24 well plate, put the section on top flat and let them thaw together at room temperature or 4 degrees. This gives the tissue a nice, slow fixation. Any other suggestions? How about working with the RNA? I'm thinking of using RNAlater-ice for example. Does anyone know if this screws up native fluorescence of GFP? Thanks! Caroline Bass ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Part-time Lab opportunity in Scottsdale, AZ
Hi all. We have a part-time job opportunity for someone interested primarily in accessioning and grossing skin specimens at our in-house dermatopathology lab (North Scottsdale Dermatology). A small amount of general lab management will also be required (ie, maintaining CLIA logs, ordering supplies etc). The hours needed will likely be Monday, Wednesday and Thursday mornings (~5 or 6am-9am). The start date will be around late December. The hours and start date are flexible, and the workload is very reasonable. Qualifications are either HT certification, or at a minimum an Associate's Degree in a health-related field with college-level credit in Chemistry, Biology, and Math, in addition to work experience in grossing and accessioning skin specimens. If interested, please respond to me by email and include a CV and/or description of your qualifications. Thanks! Carlos Rodriguez, MD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin Tissue Crumbles
Hello Histonet Users, I have just started using paraffin and am having many difficulties. Most of the time my tissue crumbles when sectioning. I have no real experience in paraffin histology and have been given the task of becoming proficient by myself, so I am hoping for feedback as to why my tissue keeps crumbling. The tissue in question has been: skeletal muscle, cardiac muscle, liver, and brain (all from rat). The tissue was fixed in 10% neutral buffered formalin for 7 days at 4°C and then transferred to an automated tissue processor, with the following schedule: 2 hours 70% dehydration alcohol 2 hours 80% dehydration alcohol 2 hours 95% dehydration alcohol 2.5 hours 95% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol .5 hour Hemo-De .5 hour Hemo-De .5 hour Hemo-De 1 hour paraffin 4 hours paraffin They were infiltrated for 1 hour without vacuum then embedded. The blocks were stored in the freezer before cutting. The knife angle was 5°. Sections were 5µm thick. I would appreciate any feedback whatsoever. Thank you very much. Michael ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
