RE: [Histonet] Distance Learning
Hi Phyllis We offer an on line, distance learning program from Columbus State Community Colllege that is fully accredited by NAACLS. It awards a certificate in histotechnology and meets the training and eligibility requirements for the ASCP HT registry examination. The certificate program is linked to an Associates degree in the Department of Allied and Multi-competency Health for those wanting a degree option. We are currently accepting students for the next program year. Our application and admissions process and application form is available from our program web page at www.cscc.edu/histology We are looking to expand our market and meet the needs of students and laboratories outside of Ohio. We have a few clinical affiliates in the Midwest, other than Ohio, and we would like to extend this further into under-served areas, ( as far as histology schools). We are able to offer 24/7 access to materials, instruction in theory and practice, practical work assessment, and interactive delivery via Blackboard learning platform. In the future we plan to offer continuing education opportunities that are histology specific to those aready certified or who may participate in the ASCP- CMP program ,and want to meet the continuing education requirements needed for participation. If I may answer any questions about histology training, certification or the on line study of histology, please feel free to contact me at jweav...@cscc.edu or at 614-287-2217. Joelle Weaver HTL (ASCP) Date: Mon, 8 Nov 2010 07:26:59 -0800 From: dch...@yahoo.com To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] Distance Learning We are thinking of using one of the online or distance learning programs to help in the training of new histotechs. Has anyone had any experience with any of these programs, what colleges offer the program, pros, cons. Any feedback is welcome. Thanks Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Oil Red O trivia
Hello Just wondering if anyone knows the what the 'O' in oil red O stands for. I suspect that it stands for nothing other than O similar to the 'G' in Orange G. One of our pathologists is looking for a tricky stain question to annoy trainees with. Thanks for your help Finlay ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Oil Red O trivia
So, the pathologist does not know the answer but he wants to annoy trainees? Glad I don't work for him! Geoff Finlay Finlay wrote: Hello Just wondering if anyone knows the what the 'O' in oil red O stands for. I suspect that it stands for nothing other than O similar to the 'G' in Orange G. One of our pathologists is looking for a tricky stain question to annoy trainees with. Thanks for your help Finlay ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Oil Red O control
Hello - How does everyone handle having a positive control for Oil Red O - We very infrequently have this stain and have had difficulty keeping control on hand. Thank you Anna CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sending Slides Out in St. Louis, MO
Hello, A friend of mine who has a brand new laboratory in St. Louis, MO currently has their histotech prepare their slides but are looking for a laboratory to have them read at because they do not have a Dermatopathologist. Please contact me if you have lab in the area who would be interested in doing that for them. Thank you! -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Oil Red O control
There was a discussion on this recently, so you may want to search the archives for it. In my opinion, the easiest and best control to use is Mayo. Just smear it on a slide as you need it and there you go. Cheap and easy. Drew On Wed, Nov 10, 2010 at 09:45, Inman, Anna anna.in...@stmarygj.org wrote: Hello - How does everyone handle having a positive control for Oil Red O - We very infrequently have this stain and have had difficulty keeping control on hand. Thank you Anna CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Oil Red O control
Hello Histonet Users, I have a question about Oil Red O staining. Could the Oil Red O solution(Poly Scientific, Cat.No. S1848) be reused? I would appreciate any feedback whatsoever. Thank you very much. Cathy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Drew Meyer Sent: Wednesday, November 10, 2010 9:55 AM To: Inman, Anna Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Oil Red O control There was a discussion on this recently, so you may want to search the archives for it. In my opinion, the easiest and best control to use is Mayo. Just smear it on a slide as you need it and there you go. Cheap and easy. Drew On Wed, Nov 10, 2010 at 09:45, Inman, Anna anna.in...@stmarygj.org wrote: Hello - How does everyone handle having a positive control for Oil Red O - We very infrequently have this stain and have had difficulty keeping control on hand. Thank you Anna CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin Tissue Crumbles
Having some experience processing and embedding rodent tissues myself (by hand), I would say that you are over-dehydrating the tissues. Try cutting back the alcohol incubation times to 30 min or even 10 min each. Regards, Merced --On Tuesday, November 09, 2010 2:56 PM -0800 Michael Mashore mmash...@vapop.ucsd.edu wrote: Hello Histonet Users, I have just started using paraffin and am having many difficulties. Most of the time my tissue crumbles when sectioning. I have no real experience in paraffin histology and have been given the task of becoming proficient by myself, so I am hoping for feedback as to why my tissue keeps crumbling. The tissue in question has been: skeletal muscle, cardiac muscle, liver, and brain (all from rat). The tissue was fixed in 10% neutral buffered formalin for 7 days at 4°C and then transferred to an automated tissue processor, with the following schedule: 2 hours 70% dehydration alcohol 2 hours 80% dehydration alcohol 2 hours 95% dehydration alcohol 2.5 hours 95% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol .5 hour Hemo-De .5 hour Hemo-De .5 hour Hemo-De 1 hour paraffin 4 hours paraffin They were infiltrated for 1 hour without vacuum then embedded. The blocks were stored in the freezer before cutting. The knife angle was 5°. Sections were 5µm thick. I would appreciate any feedback whatsoever. Thank you very much. Michael ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Tissue Crumbles
I agree most of your times are too long, but you can still get sections if you put water on your ice block and allow the tissue to rehydrate a bit or put a little ammonium hydroxide and water on your ice and sit your blocks to be section in this solution. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced M Leiker Sent: Wednesday, November 10, 2010 9:17 AM To: Michael Mashore; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Tissue Crumbles Having some experience processing and embedding rodent tissues myself (by hand), I would say that you are over-dehydrating the tissues. Try cutting back the alcohol incubation times to 30 min or even 10 min each. Regards, Merced --On Tuesday, November 09, 2010 2:56 PM -0800 Michael Mashore mmash...@vapop.ucsd.edu wrote: Hello Histonet Users, I have just started using paraffin and am having many difficulties. Most of the time my tissue crumbles when sectioning. I have no real experience in paraffin histology and have been given the task of becoming proficient by myself, so I am hoping for feedback as to why my tissue keeps crumbling. The tissue in question has been: skeletal muscle, cardiac muscle, liver, and brain (all from rat). The tissue was fixed in 10% neutral buffered formalin for 7 days at 4°C and then transferred to an automated tissue processor, with the following schedule: 2 hours 70% dehydration alcohol 2 hours 80% dehydration alcohol 2 hours 95% dehydration alcohol 2.5 hours 95% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol .5 hour Hemo-De .5 hour Hemo-De .5 hour Hemo-De 1 hour paraffin 4 hours paraffin They were infiltrated for 1 hour without vacuum then embedded. The blocks were stored in the freezer before cutting. The knife angle was 5°. Sections were 5µm thick. I would appreciate any feedback whatsoever. Thank you very much. Michael ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. == ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sending Slides Out in St. Louis, MO
Hi Alyssa, We are not in St Louis but I work for a really great Dermpath here in Colorado who might read them for you. People are not an interruption of our business. People are our business. Stacey Langenberg HT (ASCP) QIHC Laboratory Manager Histology/IF CU Dermatopathology Consultants 1999 N. Fitzsimons Pkwy Suite 120 Aurora, CO 80045 Lab-720-859-3559 Fax- 303-344-0789 Office- 303-577-2303 Cell-970-405-7742 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Alyssa Peterson [aly...@alliedsearchpartners.com] Sent: Wednesday, November 10, 2010 7:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sending Slides Out in St. Louis, MO Hello, A friend of mine who has a brand new laboratory in St. Louis, MO currently has their histotech prepare their slides but are looking for a laboratory to have them read at because they do not have a Dermatopathologist. Please contact me if you have lab in the area who would be interested in doing that for them. Thank you! -- Alyssa Peterson, Director of Candidate Recruitment LinkedIN:http://www.linkedin.com/in/alyssapetersonasp Allied Search Partners T: 888.388.7571 F: 888.388.7572 www.alliedsearchpartners.com This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Position - Muscle Enzyme Histochem Tech
Hello Histonetters: We presently have a position available for a HT/HTL (certified, or eligible) in our clinical muscle enzyme histochemistry lab (within the Department of Biomedical Research). We request 3 yrs experience minimum in EHC... and skills for manual IF, as well. If you meet these requirements and have interest, please contact cbar...@nemours.org. We are looking for you! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NH Histology Job Opportunity
Hi Histonet Members, I am currently working on a Histology Supervisor and Histology Technologist job opportunity in Southern NH. These job opportunities are with a leading national diagnostic company, which has won many awards throughout the years and has really built up their business over the past couple years (even in a tough economy). My client is willing to assist with relocation expenses if necessary and offers top notch salray and benefits. If interested in learning more, please email me your resume to ali...@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Laboratory Manager position in MI
Hi Histonet Members, I am currently working on a Laboratory Manager position in MIY. the ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in dermatopathology. This job opportunity is with a leading national diagnostic company, which has won many awards throughout the years and has really built up their business over the past couple years (even in a tough economy). My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to ali...@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Supervisor Position in GA
Hi Histonet Members, I am currently working on a Histology Supervisor position in GA. The ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in all areas of the histology lab. The candidate must also be interested/willing to live in a rural area but work in a large teaching hospital (600+ beds). My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to ali...@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Supervisor Job in Oklahoma
Hi Histonet Members, I am currently working on a Histology Supervisor position in OK. The ideal candidates must have strong supervisory/management experience, be HT ot HTL(ASCP certified, and have strong experience in all areas of the histology lab. My client is willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to ali...@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech Job Opportunities in NH, NY, NV, TN
Hi Histonet Members, I am currently working on a several histotech job opportunities in various areas across the US. I have job opportunities in NH, NY, MI, OK, GA, NV, TN, and MA. My clients are willing to assist with relocation expenses if necessary and offers top notch salary and benefits. If interested in learning more, please email me your resume to ali...@ka-recruiting.com. I will then be in touch with you to discuss this opportunity. Thanks! Sincerely, Alisha (Taylor) Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Distance Learning
Message: 8 Date: Mon, 8 Nov 2010 07:26:59 -0800 (PST) From: Phyllis Thaxton dch...@yahoo.com Subject: [Histonet] Distance Learning To: Histonet@lists.utsouthwestern.edu Message-ID: 718649.88511...@web43512.mail.sp1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 We are thinking of?using one of the online or distance learning programs to help in the training of new histotechs. Has anyone had any experience with any of these programs,?what colleges offer the program, pros, cons. Any feedback is welcome. Thanks ?Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL __- Hi Phyllis- We have had several people go through the program at IUPUI (Indiana University/Purdue University at Indianapolis), the director is Debbie Wood and she does a great job!! People are prepared to take the BOR when finished with the program. I am including her contact information. demw...@iupui.edu Good Luck Nancy Schmitt HT, MLT (ASCP) United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Telomerase h-TERT
Is anyone using the h-TERT (Telomerase) antibody with success? If so, can you share your information/protocol with me. Thanks, Jerry Santiago, BS, HTL(ASCP)QIHC Pathology Technologist Shands Jacksonville jerry.santi...@jax.ufl.edu 904-244-6149 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Position - Muscle Enzyme Histochem Tech
What state? Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barone, Carol Sent: Wednesday, November 10, 2010 11:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Position - Muscle Enzyme Histochem Tech Hello Histonetters: We presently have a position available for a HT/HTL (certified, or eligible) in our clinical muscle enzyme histochemistry lab (within the Department of Biomedical Research). We request 3 yrs experience minimum in EHC... and skills for manual IF, as well. If you meet these requirements and have interest, please contact cbar...@nemours.org. We are looking for you! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histology Technical Supervisor (Chicago, IL)
Slone Partners seeks a Histology Technical Supervisor for a growing laboratory affiliated with a large Health Care Organization in a Western suburb of Chicago. The ideal candidate will be HTL certified with a 4 year degree and management or supervisory experience in a busy histology laboratory. Please note that this client requires a minimum of a 4 year degree to be considered for this role. Special feature of this position: This client offers competitive wages, superior benefits and opportunities for advancement. If you meet these requirements and wish to be considered for this position, submit your resume to Darcy Bloch at dar...@slonepartners.com mailto:dar...@slonepartners.com?subject=new%20candidate%20request%20%28hist ology%20Technical%20Supervisor%20-%201462%29 . If you have experience in the diagnostic laboratory industry and wish to be considered for other roles, please forward your resume to Tara Kochis at mailto:t...@slonepartners.com?subject=new%20candidate%20request t...@slonepartners.com. All inquiries are kept confidential ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hi Histonetters, This maybe a stupid question but I'm going to ask anyways. Is it legal to buy tissue blocks (with human tissue) from another lab to use as controls for specials and Immunos? I know we purchase slides from vendors to use with our specials and Immunos. Thanks! Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Oil Red O control
Once you mix your stock solution with water, it is good for only about an hour. Then you have to discard it. Michelle Aloni MS HTL (ASCP) USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HSP-90 (beta)
Does anyone have experience with an antibody to Heat shock protein-90 (beta) for IHC? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Correcting van Gieson over-stain
Hi folks, We have a set of slides that we stained in van Gieson's solution, and unfortunately, they turned out too dark. I tried immersion in 100% ethanol for ~2 minutes, and that helped a little, but they are still too dark. Does anyone have any experience or suggestions as to what solution to use to better differentiate the staining? Thanks in advance for all of your suggestions! ~Sean ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Position available
Hello Histonetters: We presently have a position available for a HT/HTL (certified, or eligible) in our clinical muscle enzyme histochemistry lab (within the Department of Biomedical Research). We request 3 yrs experience minimum in EHC... and skills for manual IF, as well. If you meet these requirements and have interest, please contact cbar...@nemours.org blocked::mailto:cbar...@nemours.org . We are looking for you! Check us out on line at www.nemours.org We are 25 minutes south of Philadelphia, on a private campus, free parking and no state sales tax! Nemours - A.I. duPont Hospital for Children Department of Biomedical Research Histotechnology Core Lab Wilmington, DE 19803 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Paraffin Tissue Crumbles
Hello to everyone, Thank you for all the advice. I will reduce my processing time as well as try to rehyrdate my paraffin blocks before cutting. I greatly appreciate the help. Thanks, Michael ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Parrafin tissues crumbles
Hi Michael. 1. Fixation : 48 hours at room T is a good time to fix a big rat heart. You can transfer at 4C after 24/48 hours, otherwise cold T from the beginning prevents good penetration and fixation. 2.. process the hearts and muscles separate from other tissues 3. use only fresh reagents in you processing machine. I always rotate reagents before process the hearts and this really works for me.2 hours in each Ethanols is OK in my opinion but according me experience it depend on processing center .I think that you use xylene substitute and I can recommend to switch back to real Xylene's. the clearing in Xylene is very short -30 Minutes?, especially after long dehydration. It would be better if you use 1.30 or 2 hours on each. Time in paraffin is OK, but I prefer 3 baths of paraffin, 1.30 or 2 hours in each. In the processing center use paraffin called infiltration medium i use from surgipath # 01400, for embedding i highly recommend Shandon Precision Cut paraffin from Thermo Shandon# B1002490. Do not freeze paraffin blocks this make the paraffin and tissues very fragile. You can cool the blocks in refrigerator or on the top of cold plate. Trim the paraffin block and place in the DI water for soaking for 5-6 minutes, do not keep to long. Use only DI water in the floating bath. Moisten the cutting surface of the block with your finger during sectioning, this is help to get more smooth sections. My English is not perfect but I hope you understand my tips.please contact with me if you have additional questions. Galina Deyneko Novartis, Cambridge, MA 617-871-7613 galinadeyn...@yahoo.com Message: 8 Date: Tue, 9 Nov 2010 14:56:49 -0800 From: Michael Mashore mmash...@vapop.ucsd.edu Subject: [Histonet] Paraffin Tissue Crumbles To: histonet@lists.utsouthwestern.edu Message-ID: 652586e049884d8491a0e70448595...@vandeberg Content-Type: text/plain; charset=iso-8859-1 Hello Histonet Users, I have just started using paraffin and am having many difficulties. Most of the time my tissue crumbles when sectioning. I have no real experience in paraffin histology and have been given the task of becoming proficient by myself, so I am hoping for feedback as to why my tissue keeps crumbling. The tissue in question has been: skeletal muscle, cardiac muscle, liver, and brain (all from rat). The tissue was fixed in 10% neutral buffered formalin for 7 days at 4°C and then transferred to an automated tissue processor, with the following schedule: 2 hours 70% dehydration alcohol 2 hours 80% dehydration alcohol 2 hours 95% dehydration alcohol 2.5 hours 95% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol 2 hours 100% dehydration alcohol .5 hour Hemo-De .5 hour Hemo-De .5 hour Hemo-De 1 hour paraffin 4 hours paraffin They were infiltrated for 1 hour without vacuum then embedded. The blocks were stored in the freezer before cutting. The knife angle was 5°. Sections were 5µm thick. I would appreciate any feedback whatsoever. Thank you very much. Michael -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Oil Red O control
Cut frozen section of adrenal from an autopsy or surgical pathology. Store slides in a box in the refrig. Good for a year. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -- From: Inman, Anna anna.in...@stmarygj.org Sent: Wednesday, November 10, 2010 9:45 AM To: Geoff McAuliffe mcaul...@umdnj.edu; Finlay Finlay f.fin...@formed.gla.ac.uk Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil Red O control Hello - How does everyone handle having a positive control for Oil Red O - We very infrequently have this stain and have had difficulty keeping control on hand. Thank you Anna CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ThinPrep vs. SurePath
Does anyone have an opinion as to why there seems to be many more ThinPreps out there than Sure Path? The SurePath seems more efficient, but I might be missing something. Thank you, Jennifer MacDonald ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
