[Histonet] IHC antibody protocols
Hello All, I need help with information on a few Ventana BenchMark Ultra or XT protocols. We are having trouble getting HCL (DBA.44) to work. We are also in the process of working up FOXP-1, TAI-1, and GCET-1. Your help is appreciated. Thank you, Matt Brooks, BS, HT (ASCP) Histology Supervisor InCyte Pathology mbro...@incytepathology.com 509-892-2744 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Frozen brain slices
Thanks Tina. I will keep in mind the thickness for staining purposes that is once the slicing protocol gets optimized. So we use 200-300 ml of both PBS and PFA for our perfusion. So the post fix and sucrose treatment you are suggesting, are you suggesting for the new harvests alone which I will be doing or the existing frozen perfused ones? Anjum On Sun, Nov 14, 2010 10:35 PM, Montina Van Meter montina.vanme...@pbrc.edu wrote: Anhum, How much PBS and Paraformaldehyde do you put through the (rat or mouse). I flush a perfuse our rats with 150-200ml. of PBS and 500ml. of Para. Postfix for 2 hours and place in 20% sucrose overnight until the tisue sinks to the bottom of the vial. The morphology of your tissue is going to be compromised due to freeze artifact (lack of cryoprotectant) and there will be holes in your sections. Actually, 30um thick sections are considered quite thick and are typically used for free floating techniques. Sections that are mounted on slides before IHC staining are much thinner (3-10um). I usually cut my tissue between 30-40um and manually free float the sections for IHC or IF. Tina Sent from my iPhone On Nov 14, 2010, at 9:05 PM, ANJUM PARKAR axp...@psu.edu wrote: Thanks Tina. I will keep the sucrose suggestion in mind for future harvests. For now I need to figure out how to slice the already fixed frozen tissue. Will try to equilibrate in the cryostat itself as against room temperature and see how that goes. The knife angle is 13 degrees and recently sharpened and thickness of slices 30 microns which I believe is standard for sectioning. I have sectioned previously a lot so doing hands on is something I am familiar with. But I did realize every instrument is different and while doing a procedure it always needs to be optimized until it can become routine. Will let you know how things go. Anjum On Sun, Nov 14, 2010 02:21 PM, Montina Van Meter montina.vanme...@pbrc.edu wrote: Anhum, 1. You MUST cryoprotect the fixed tissue in 15-30% sucrose (until it sinks) prior to cutting frozen sections. 2. Allow the -80C embedded tissue block to equilabrate to -20C in the cryostat for 15-20min. 3. Check the knife angle. Thickness? 4. Change knife or sharpen and make sure all screws are tight. 5. Freez/thawing of block is not a good idea (especially since it's not cryoprotected). You are introducing ice crystal artifact. 6. Check around your department (or Histology Core) to see if someone can instruct you on using the cryostat. It's always better to actually watch someone in addition to receiving written instructions. Good luck, Tina Sent from my iPhone On Nov 14, 2010, at 10:47 AM, ANJUM PARKAR axp...@psu.edu wrote: Hello all, I have been having some histology issues in terms of rat brain slicing the past few months and hence looking for suggestions to fix them, having tried most of what I know from my past training and running of ideas real fast now. The following is the protocol I have used thus far:-1. Animal perfusion-a)Use PBS first, b) Use 4%PFA next and c) Harvest brain right after PFA. (Transcardial perfusion)2. Freeze brain by embedding in OCT compound using dry ice and then storing at -80 until ready to slice. Alternatively some times the -80 is used straight up with out dry ice freezing (still embedded in OCT).3. From standard protocol I do not do two things-a) No post fixation once brain is harvested and b) No sucrose. Reason being, I was taught that these two steps might result in large holes in tissue.4.For slicing, we have the older cryostat version (Real old version of CM3050S from Vibrotome-30 micron slices) which uses a fixed knife for slicing and has a chamber temp control and one for the specimen. The cryostat has not been serviced for a few years now.5. In attempt 1-I take the brain out of the -80 once the chamber temperature comes to -20 and mount the frozen mold on the chuck and attempt at slicing. Doing this I managed to get a few slices (that too with cuts and ripples) and after which the tissue just kept cracking and falling into pieces and hence could get no more slices.6. In attempt 2-I thought the brain may be too cold and too hard (perfusion and-80 freezing), so I defrosted the brain for 20 mins at room temperature before sticking into -20 cryostat. Between slices if I felt the tissue was too cold, I put my thumb on the specimen to warm it up. Still no luck.7. In attempt 3-I defrosted the brain completely to room temperature, took the brain out of the cryogel embedding (thinking that the mold was way too hard to cut through and hence cracking the tissue too) and attached it to chuck directly and tried slicing. Still no luck at all this time around. From what I have been reading online and from what I know, I primarily feel these are issues related with temperature of the brain and that it is too cold during
RE: [Histonet] ThermoFisher's PrintMate (cassette printer) and Slide Mate (slide labeler with scanner)
We are using both. They both have had their fair share of problems, but overall I think the improved efficiency is worth working out the kinks. I recommend both. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Damaris Beil Sent: Saturday, November 13, 2010 6:42 AM To: histonet Subject: [Histonet] ThermoFisher's PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) Hello, I'm interested in finding out if anyone is using ThermoFisher's new PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) and how they are working out for you. Thanks in advance for your help, Damaris ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Annual Review of Manuals
For those of you who work in/manage CAP accredited laboratories, what is your current understanding/policy in regards to annual review of all relevant procedure manuals by all personnel? If you no longer do this, what procedure manual review IS done in your lab? Thanks, Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] prostate biopsies
Can anyone share their processing schedules for prostate biopsies using a variety of processors? Examples: Sakura VIP, Tissue Tek express, Leica Peloris, or Thermo Pathcentre? What is the optimum rapid processing schedule? Do you prefer to use a certain kind of alcohol? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Procedure manual review
All procedures must be reviewed annually (and that means WITHIN 12 months, not just once a calender year) This may be done by the appropriate supervisor or pathologist, not necessarily the Director (Director must review all new or changed procedures) The review should confirm that the procedure in operation matches what really happens. The laboratory must have in place a mechanism that demonstrates that the users of those procedures are familiar with them (and without digging up the CAP standards, i rely on my memory that this must also be documented in some format on an annual basis). I believe we accomplish this with an annual signed statement from the employees confirming they are familiar with the procedures they use. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ThermoFisher's PrintMate (cassette printer) and SlideMate (slide labeler with scanner)
I'm looking at the Slide Mate and Print Mate, too. Currently, I have the Leica cassette and slide printers. They have really been great. Reliable and relatively problem free. But they are so large. I'd like to find a slide printer that has a small footprint, like the Slide Mate, so that we can place one at each microtomy station. Assuming there are other slide printers out there, besides ThermoFisher's Slide Mate and Leica's, what are their pro's and con's? Sandy Harrison -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Monday, November 15, 2010 9:29 AM To: Damaris Beil; histonet Subject: RE: [Histonet] ThermoFisher's PrintMate (cassette printer) and SlideMate (slide labeler with scanner) We are using both. They both have had their fair share of problems, but overall I think the improved efficiency is worth working out the kinks. I recommend both. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Damaris Beil Sent: Saturday, November 13, 2010 6:42 AM To: histonet Subject: [Histonet] ThermoFisher's PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) Hello, I'm interested in finding out if anyone is using ThermoFisher's new PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) and how they are working out for you. Thanks in advance for your help, Damaris ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tap Water or filtration system
We are in the process of getting our new Lecia Autostainer delivered to us. The question came up if we will still need to continue using our water purification system for the stainer. Mostly the docs would like to save money but I don't want them to be unhappy with the result. I would like to hear all the pros and cons of discontinuing/continuing the use of our system and going w/ tap water from the sink. What is the PH level supposed to test at? What difference will I see in the slides? By the way, I only stain HE at the current time but there is a possibility of starting PAS or other special stains. Thank you in advance!!! V.Avalos ADS, INC Fax:602-277-2134 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] glutaraldehyde fixed tissues and genomic DNA
Hello, I have PFA+glutaraldehyde fixed tissues that I might want to digest down in order to extract genomic DNA suitable for bisulfite sequencing. The tissues are embryonic mouse heads. The fixative is a PFA + glutaraldehyde mix: 3.6 PFA with 5% glut. The glut is grade 1 or electron miccroscopy grade. I'd like to pro-K the tissues and then phenol-chloroform extract genomic DNA. My hope is that the amount and quality of the DNA will be suitable for bisulfite conversions for methylation anaylsis. Will the DNA be abundant? Will it be fairly protein free (does the fixative fix protein to the DNA?). Thanks Eric University of Calgary Facutly of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ThermoFisher's PrintMate (cassette printer) and SlideMate (slide labeler with scanner)
I agree with Laurie. We have seen a great improvement in efficiency and reduction of errors with this hardware. We have been using both for a few months now and have not had many problems. Jaime McMillan -Original Message- From: Laurie Colbert [mailto:laurie.colb...@huntingtonhospital.com] Sent: Monday, November 15, 2010 7:29 AM To: Damaris Beil; histonet Subject: RE: [Histonet] ThermoFisher's PrintMate (cassette printer) and SlideMate (slide labeler with scanner) We are using both. They both have had their fair share of problems, but overall I think the improved efficiency is worth working out the kinks. I recommend both. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Damaris Beil Sent: Saturday, November 13, 2010 6:42 AM To: histonet Subject: [Histonet] ThermoFisher's PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) Hello, I'm interested in finding out if anyone is using ThermoFisher's new PrintMate (cassette printer) and Slide Mate (slide labeler with scanner) and how they are working out for you. Thanks in advance for your help, Damaris ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Non-Refrigerated RPMI/3-in-1 Pap Stain
I would like to obtain information to order the non-refrigerated RPMI. And does a 3-in-one Pap stain exist? Someone in a workshop in Seattle said they knew about it but couldn't give the exact supplier. Thank you for your help. M. Fisher El Centro Regional Medical Center www.ecrmc.org http://www.ecrmc.org Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. Â ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tap Water or filtration system
I don't have the Lecia Autostainer but have a Surgipath Tribune stainer. I have it connected to tap water and do both HE and PAS on it. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Avalos Sent: Monday, November 15, 2010 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tap Water or filtration system We are in the process of getting our new Lecia Autostainer delivered to us. The question came up if we will still need to continue using our water purification system for the stainer. Mostly the docs would like to save money but I don't want them to be unhappy with the result. I would like to hear all the pros and cons of discontinuing/continuing the use of our system and going w/ tap water from the sink. What is the PH level supposed to test at? What difference will I see in the slides? By the way, I only stain HE at the current time but there is a possibility of starting PAS or other special stains. Thank you in advance!!! V.Avalos ADS, INC Fax:602-277-2134 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tap Water or filtration system
We use tap water on our Leica. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, November 15, 2010 13:15 To: 'Vanessa Avalos'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Tap Water or filtration system I don't have the Lecia Autostainer but have a Surgipath Tribune stainer. I have it connected to tap water and do both HE and PAS on it. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Avalos Sent: Monday, November 15, 2010 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tap Water or filtration system We are in the process of getting our new Lecia Autostainer delivered to us. The question came up if we will still need to continue using our water purification system for the stainer. Mostly the docs would like to save money but I don't want them to be unhappy with the result. I would like to hear all the pros and cons of discontinuing/continuing the use of our system and going w/ tap water from the sink. What is the PH level supposed to test at? What difference will I see in the slides? By the way, I only stain HE at the current time but there is a possibility of starting PAS or other special stains. Thank you in advance!!! V.Avalos ADS, INC Fax:602-277-2134 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide Mates and Cassette printers
We have three of the older six hopper Thermo Micro cassette writers and one Thermo slide mate, in addition to four of the Pslims. The Pslims are the first generation Slide Mate by Accuplace. They are all connected to our APLIS System (PowerPath) and we use 2D bar-codes on our specimen labels, cassettes and slides. We do not produce a cassette or slide without wanding a bar-code. The printed specimen bar codes that generate the cassettes and bar-coded cassettes that generate the slides for positive patient identification, out weighs the manual human method is well worth the effort. This is where all histology lab should be moving towards. We have some little network issue with them, but for the most part they print great. The cassette printers work well. Remember these are mechanical and computer driven, so they will fail at times. Please feel free to contact me if you have any questions. Rick Garnhart HT(ASCP) Memorial Health System Histology Supervisor 1400 E. Boulder St. Colorado Springs, CO 80909 Cell: 719-365-8357 Ph: 719-365-6926 Fax: 719-365-6373 rick.garnh...@memorialhealthsystem.com Mission: To provide the highest quality health care Vision: To create an outstanding health system where patients heal and people thrive Values: Compassion - Integrity - Quality - Respect - Teamwork www.memorialhealthsystem.com The information contained in or attached to this electronic message is privileged and confidential, intended only for the use of the individual(s) named above. If the reader of this message is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please inform the sender immediately and remove any record of this message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tap Water or filtration system
We always used tap water, without any problems. Vanessa Avalos vava...@allergydermatology.com Sent by: histonet-boun...@lists.utsouthwestern.edu 11/15/2010 09:54 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Tap Water or filtration system We are in the process of getting our new Lecia Autostainer delivered to us. The question came up if we will still need to continue using our water purification system for the stainer. Mostly the docs would like to save money but I don't want them to be unhappy with the result. I would like to hear all the pros and cons of discontinuing/continuing the use of our system and going w/ tap water from the sink. What is the PH level supposed to test at? What difference will I see in the slides? By the way, I only stain HE at the current time but there is a possibility of starting PAS or other special stains. Thank you in advance!!! V.Avalos ADS, INC Fax:602-277-2134 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] pathologists
Would any of the pathologists or histotechs know what the average volume of work for a urology pathologist would be for a given day? How many slides/cases is a comfortable workload for one day of reading, say in regard to prostate biopsies or bladder biopsy cases (excluding vas deferens)? Also including immunos if any. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Mohs
Hi Everyone!! I need advise on getting started with IHC on Mohs sections! Vendors, automated vs by hand, antibodiesanything and everything will be s greatly appreciated. Please and Thank You in advance!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE:Tap Water or filtration system
What if we suggest to use ddH2O both for TBST preparation and in autostainers (before and after IHC) because the PH level is vary in different states. Naira ** Confidentiality Note: This message (including any attachments) is intended for the use of the named recipient(s) only and may contain confidential and/or proprietary information. If you are not the intended recipient, please contact the sender and delete this message. Any unauthorized use of the information contained in this message is prohibited. -Original Message- Message: 11 Date: Mon, 15 Nov 2010 10:50:50 -0700 From: Vanessa Avalos vava...@allergydermatology.com Subject: [Histonet] Tap Water or filtration system To: histonet@lists.utsouthwestern.edu Message-ID: 000e01cb84ed$a49cdbd0$edd693...@com Content-Type: text/plain; charset=us-ascii We are in the process of getting our new Lecia Autostainer delivered to us. The question came up if we will still need to continue using our water purification system for the stainer. Mostly the docs would like to save money but I don't want them to be unhappy with the result. I would like to hear all the pros and cons of discontinuing/continuing the use of our system and going w/ tap water from the sink. What is the PH level supposed to test at? What difference will I see in the slides? By the way, I only stain HE at the current time but there is a possibility of starting PAS or other special stains. Thank you in advance!!! V.Avalos ADS, INC Fax:602-277-2134 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] pathologists
It is important to understand details of slide preparation. We prepare six levels of each block/ each glass slide. A case is prostate biopsies from one patient. 85% of the time we get two biopsy cores per container and thus two cores per block, and 12 cores total per case. A busy day is 12 biopsy cases, 6 slides each, with six levels of each block (containing two biopsy cores) on each slide). I am aware that some pathologists deal with as many as 20 prostate biopsy cases per day. The detail of how the slides are prepared and screened can differ from place to place. The experience of the pathologist also affects throughput. The only measure we really have of QA is prevalence (i.e. are we missing cases). Prevalence of prostate cancer in cases varies surprisingly ranging from high 20s to high 45%. I've been in two different sites in which 45-50% of cases are positive. The patient population being screened and biopsied obviously would impact the prevalence rate. Dean Troyer From: histonet-boun...@lists.utsouthwestern.edu on behalf of Johannes Koepplinger Sent: Mon 11/15/2010 3:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pathologists Would any of the pathologists or histotechs know what the average volume of work for a urology pathologist would be for a given day? How many slides/cases is a comfortable workload for one day of reading, say in regard to prostate biopsies or bladder biopsy cases (excluding vas deferens)? Also including immunos if any. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Control sections for frozen sections
Does anyone run a HE control prior to staining a frozen section? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tap Water or filtration system
We use tap water and have had no problems. Sydney tap water seems to be quite innocuous Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Avalos Sent: Tuesday, 16 November 2010 4:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tap Water or filtration system We are in the process of getting our new Lecia Autostainer delivered to us. The question came up if we will still need to continue using our water purification system for the stainer. Mostly the docs would like to save money but I don't want them to be unhappy with the result. I would like to hear all the pros and cons of discontinuing/continuing the use of our system and going w/ tap water from the sink. What is the PH level supposed to test at? What difference will I see in the slides? By the way, I only stain HE at the current time but there is a possibility of starting PAS or other special stains. Thank you in advance!!! V.Avalos ADS, INC Fax:602-277-2134 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tap Water or filtration system
We use tap water in our Leica multistainer for HE's and a few other stains. The protocols can be optimized to accommodate what ever you are using. I can't speak to the PAS stain except to say that even if you need deionized water, you can buy it in 5 gallon cubes for limited use and still save money. Steve -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Avalos Sent: Monday, November 15, 2010 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tap Water or filtration system We are in the process of getting our new Lecia Autostainer delivered to us. The question came up if we will still need to continue using our water purification system for the stainer. Mostly the docs would like to save money but I don't want them to be unhappy with the result. I would like to hear all the pros and cons of discontinuing/continuing the use of our system and going w/ tap water from the sink. What is the PH level supposed to test at? What difference will I see in the slides? By the way, I only stain HE at the current time but there is a possibility of starting PAS or other special stains. Thank you in advance!!! V.Avalos ADS, INC Fax:602-277-2134 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cardboard slide flat storage
Hi Meghan, We use grey colored boxes that will hold either 8 cardboard drawers of blocks or 4 cardboard drawers of slides. I am at home right now, don't have the specific ordering information, but will share it tomorrow when I get to work. I just didn't want to let the question languish w/o some sort of response! Michelle On Nov 15, 2010, at 9:30 AM, Meghan Tucker meghan.tuc...@yahoo.com wrote: Good morning, Are there any suggestions on a place to order a storage shelf for cardboard slide flats, which may have 6-8 'cubbies' that can be used to organize the flats? Thanks! Meghan meghan.tuc...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
