[Histonet] Histotechnologist position in DC
Slone Partners seeks a Histotechnologist for a cutting edge hospital laboratory, based in a beautiful Washington DC neighborhood. ASCP certification is preferred with at least 2 years of experience in a high-volume laboratory. Special features of the position: This organization embraces curiosity and enjoys mentoring and developing its employees. Interested and qualified candidates should submit their resume to Kim Wilson at k...@slonepartners.com. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Request for Coverslip Removal SOP
Hi colleagues, I need to remove a glass coverslip from a mounted HE biopsy slide with an implant in order to run some biomaterial surface characterization studies. Does anyone have a SOP that they would be willing to share for removing glass coverslips without damaging the specimen? Thanks in advance for all of the great advice that I always get from the histonet. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbr...@andrew.cmu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Antigen Retrieval for 10u sections in IHC
Hi tistonetters, I have to do IHC on 10µ sections. Is procedure for Antigen Retrieval same like for 4-5µ (time and temperature) Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Request for Coverslip Removal SOP
soaking in xylene for at least a couple of hours (overnight is best) works for us. On Fri, Jan 14, 2011 at 11:55 AM, Sean McBride smcbr...@andrew.cmu.eduwrote: Hi colleagues, I need to remove a glass coverslip from a mounted HE biopsy slide with an implant in order to run some biomaterial surface characterization studies. Does anyone have a SOP that they would be willing to share for removing glass coverslips without damaging the specimen? Thanks in advance for all of the great advice that I always get from the histonet. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbr...@andrew.cmu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Request for Coverslip Removal SOP
Hi, I don't have an actual SOP but we just soak in a jar of xylene until the coverslip come off. Lisa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sean McBride Sent: Friday, January 14, 2011 1:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Request for Coverslip Removal SOP Importance: High Hi colleagues, I need to remove a glass coverslip from a mounted HE biopsy slide with an implant in order to run some biomaterial surface characterization studies. Does anyone have a SOP that they would be willing to share for removing glass coverslips without damaging the specimen? Thanks in advance for all of the great advice that I always get from the histonet. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbr...@andrew.cmu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] decalcifying bone marrows after processing
I was wondering if anyone has any experience with, or is it even possible to, decalcify bone marrows after they are processed. Our pathologist would like to be able to process bone marrows the same day we receive them, but most of the time there just isn't enough time to allow for proper fixation and then proper decalcification. She asked if we could process them and then decalcify and I have yet to find an answer to this question. Please help!! Thank you, Kerry Powers Comanche Country Memorial Hospital Department of Pathology 3401 W Gore, Lawton OK 73505 (580) 355-8699 ext. 3359 Fax: (580) 585-5462 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] decalcifying bone marrows after processing
If you what to do a histology work of quality, you cannot decalcify after processing, besides, what is the point? It is preferable to use formic acid (even if it is worse than using EDTA) than having to struggle with a poor section produced and then trying to decalcify it. This is typical of the ignorance of most pathologists about tissue processing things. René J. --- On Fri, 1/14/11, Powers, Kerry powe...@ccmhonline.com wrote: From: Powers, Kerry powe...@ccmhonline.com Subject: [Histonet] decalcifying bone marrows after processing To: histonet@lists.utsouthwestern.edu Date: Friday, January 14, 2011, 4:31 PM I was wondering if anyone has any experience with, or is it even possible to, decalcify bone marrows after they are processed. Our pathologist would like to be able to process bone marrows the same day we receive them, but most of the time there just isn't enough time to allow for proper fixation and then proper decalcification. She asked if we could process them and then decalcify and I have yet to find an answer to this question. Please help!! Thank you, Kerry Powers Comanche Country Memorial Hospital Department of Pathology 3401 W Gore, Lawton OK 73505 (580) 355-8699 ext. 3359 Fax: (580) 585-5462 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
