[Histonet] HT Exam
Hello everyone! Has anyone taken the HT Exam recently. I heard it was hard. Can anyone give me any help please. Heather Cooper TruPath Laboratory, LLC Leesville, LA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histotech is suppose to cut per hour?
Practice, practice, practice! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine he slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histotech is suppose to cut per hour?
I agree with Cynthia. Practice and setting up a standard system of how you cut. I do the same thing every time with each block I cut. Cutting faster is not necessarily a good thing if you are jeopardizing quality just to cut more slides. It's quality over quantity in my book any day! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Tuesday, March 01, 2011 7:51 AM To: 'Jenny Vega'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Practice, practice, practice! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine he slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histotech is suppose to cut per hour?
Worry more about quality than speed at this time. I measure my staff on quality rather than how fast things get done. It's a waste of time, energy and resources to have to re-cut blocks because of poor quality. Speed comes with time and experience. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, March 01, 2011 9:14 AM To: histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? I agree with Cynthia. Practice and setting up a standard system of how you cut. I do the same thing every time with each block I cut. Cutting faster is not necessarily a good thing if you are jeopardizing quality just to cut more slides. It's quality over quantity in my book any day! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Tuesday, March 01, 2011 7:51 AM To: 'Jenny Vega'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Practice, practice, practice! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine he slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histo tech is suppose to cut per hour?
Hello Jenny, An experienced tech should be able to cut and average of 1 block every 2.5 minutes. Embedding is 1 block per minute ave. Note this is an average. It includes difficult and easy tissue, cleaning the waterbath after each block, writing/printing the slide number, etc. There may be variations in each lab that can change these numbers. Hope this helps. Jan Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 4:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine he slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histotech is suppose to cut per hour?
I agree with Tom. Janice is correct on her timing numbers. However; when you are starting out it is best to get the flow for working at your station than beating a clock. You will gain your speed with learning accuracy which is more important for the patient at the end. We are in patient care not NASCAR and should always remember patient first, which translates to accuracy and the best sections we can produce on every slide. Speed will come as you do more blocks and get better. Pam Marcum From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas [tpodawi...@lrgh.org] Sent: Tuesday, March 01, 2011 8:26 AM To: 'Konop, Nicole'; histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Worry more about quality than speed at this time. I measure my staff on quality rather than how fast things get done. It's a waste of time, energy and resources to have to re-cut blocks because of poor quality. Speed comes with time and experience. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, March 01, 2011 9:14 AM To: histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? I agree with Cynthia. Practice and setting up a standard system of how you cut. I do the same thing every time with each block I cut. Cutting faster is not necessarily a good thing if you are jeopardizing quality just to cut more slides. It's quality over quantity in my book any day! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Tuesday, March 01, 2011 7:51 AM To: 'Jenny Vega'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Practice, practice, practice! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine he slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histotech is suppose to cut per hour?
Well said Pam. All the speed in the world means nothing if you can't produce quality slides. The suggestion to do things the same way every time is a good one. However, learning what works well on what tissue is very helpful too. Like icing something that wrinkles or soaking something that shatters, etc. It all comes from experience. I plan to retire in 2 months but will keep my foot in the door working casual so that I don't lose my skills. I love this profession! Jan Omaha -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 01, 2011 8:58 AM To: Podawiltz, Thomas; 'Konop, Nicole'; histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? I agree with Tom. Janice is correct on her timing numbers. However; when you are starting out it is best to get the flow for working at your station than beating a clock. You will gain your speed with learning accuracy which is more important for the patient at the end. We are in patient care not NASCAR and should always remember patient first, which translates to accuracy and the best sections we can produce on every slide. Speed will come as you do more blocks and get better. Pam Marcum From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas [tpodawi...@lrgh.org] Sent: Tuesday, March 01, 2011 8:26 AM To: 'Konop, Nicole'; histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Worry more about quality than speed at this time. I measure my staff on quality rather than how fast things get done. It's a waste of time, energy and resources to have to re-cut blocks because of poor quality. Speed comes with time and experience. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, March 01, 2011 9:14 AM To: histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? I agree with Cynthia. Practice and setting up a standard system of how you cut. I do the same thing every time with each block I cut. Cutting faster is not necessarily a good thing if you are jeopardizing quality just to cut more slides. It's quality over quantity in my book any day! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Tuesday, March 01, 2011 7:51 AM To: 'Jenny Vega'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Practice, practice, practice! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine he slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the
[Histonet] looking for KJ Kellogg
I am looking for KJ Kellogg, or Kjirsten Kellogg she is a HT/PA ASCP working some where in Kansas I think. KJ is originally from Wisconsin. I have an urgent, personal message for her. Thanks, Cheri Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor, Safety Officer, Physicians Laboratory Services Omaha, NE. 68127 402 731 4145 ext. 554 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide filing system search
Hi all, We are looking for a system of some sort that will allow us to group interesting cases together for quick review. We will use this for teaching, visiting scientists, etc. We want something that will allow for the slides to be stored flat and not in a vertical position, if at all possible. Anyone know of anything? We are talking about quite a few cases...maybe 100+. Vendors welcome to reply. Thanks in advance! Jeanine Bartlett, BS, HT(ASCP)QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Labelers - Needs and Must Haves
If you had the chance to pick features that are a MUST HAVE for cassette and slide labelers what would those features/must haves be? Curious to Know Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How many tissues an histotech is suppose to cut per hour?
To cut 24 single slides blcoks/hour René J. --- On Mon, 2/28/11, Jenny Vega histotech...@gmail.com wrote: From: Jenny Vega histotech...@gmail.com Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? To: histonet@lists.utsouthwestern.edu Date: Monday, February 28, 2011, 5:18 PM I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine he slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Labelers - Needs and Must Haves
The ability to print barcodes and read barcodes -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tonia Richmond Sent: Tuesday, March 01, 2011 8:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Labelers - Needs and Must Haves If you had the chance to pick features that are a MUST HAVE for cassette and slide labelers what would those features/must haves be? Curious to Know Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] I.F. Controls
Hi everyone, Just wondering what you all use as controls for I.F. kindney biopsies!! Thanks D ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Labelers - Needs and Must Haves
We just went through an evaluation for cassette printers that produce a 2D bar code. Top features we looked for: Print Accession#, last name/first initial, and 2D bar code under 10 seconds a cassette Minimal jamming/defects of printed cassettes, 1 defect/100 cassette or less Tubed or taped cassettes for hoppers for quick and easy loading Cost of cassettes available for use time and complexity of daily, weekly, monthly maintenance of instrument Ease of use by employees Interfaced to multiple LIS Compatible with work flow software and vendor supported We found that the cassette printers fell into three are types of printers available, tape/ribbon transfer, ink jet and laser etching. All three types have there pluses and minuses. William DeSalvo, B.S., HTL(ASCP) Date: Tue, 1 Mar 2011 16:35:22 + From: tjrichm...@hughes.net To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Labelers - Needs and Must Haves If you had the chance to pick features that are a MUST HAVE for cassette and slide labelers what would those features/must haves be? Curious to Know Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HELP!!!!! H. Pylori Immunos
Does anyone do H. Pylori Immunos by hand? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Charles (Chuck) Churukian Remembered
With sadness, I received this message from National Society of Histotechnology about the passing of Chuck Churukian and was asked to pass it on to Histonet. Charles J. Churukian Brighton: May 11, 1928 - February 23, 2011, Charles J. Churukian 82. Predeceased by his parents, Joseph and Christin Aintablian Churukian, and sister, Sally Churukian Taroni. He is survived by his wife, Irene Billings Churukian of 41 years and his sister, Rose Churukian Milone as well as sisters-in-law, nieces and nephews. Charles will be sadly missed by family and friends. As a PFC in the infantry, he served as a heavy machine gunner in World War II receiving the Army of Occupation Medal and Victory Medal. Teacher, mentor, poet, editor, innovator, guru of special stains, Charles worked in histology laboratories for 54 years and supervised the Histotechnology Lab at the UR Medical Center for the last 40 years. He was recently presented with the Histotechnologist of the Decade Award by the National Society of Histotechnology for his contributions to the laboratory science field having numerous publications, presentations and awards to his credit. He devoted his career looking for ways to modify the art of special stains for the benefit of patient care. In addition to his professional life, he offered spiritual guidance to many inmates at the jail. On Saturday, March 5, at 11:00, friends are invited to attend a Memorial Service at Asbury United Methodist Church at 1050 East Ave. followed by a reception in 1010 East Avenue, adjacent to the church. Interment, White Haven at the convenience of the family. Charles' family would like to thank the staff at UR Wilmot Cancer Center, and LifetimeCare Agency for their loving compassionate care In lieu of flowers, donations may be directed to Good News and Jail Ministry of the Rochester Area, AMAA Scholarship Fund (for university students in Armenia), Asbury Outreach Programs (Dining Caring Center, Storehouse) or James P. Wilmot Cancer Center. Gayle M. Callis HTL,HT,MT(ASCP) Bozeman MT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NSH - One Day VIR Forum VIR Teleconference Series
One Day VIR Forum Saturday, March 26, 2011 8:30 am - 4:15 pm Hampton Inn Suites in Raleigh, NC Sessions Include: * Digital Pathology - A New Era for Pathology presented by Curtis Adams, PhD, Sr. Product Manager, Life Sciences, Aperio * Developing, Trouble Shooting, and Optimizing IHC Detection Systems for Non-Human Tissues presented by Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC, Manager Premier Laboratory, LLC * Laser Microdissection: The Before and After presented by Diane L. Sterchi, MS, HT(ASCP)HTL, Sr. Research Associate, Covance, Inc. * Tissue Grossing Pathology: Descriptive Methods Terminology presented by Richard French, DVM, PhD, Sr. Veterinary Pathologist, University of New Hampshire Registration Fee: $119 (NSH member); $159 (non members) For workshop abstracts and registration information: http://www.goeshow.com/nsh/VIR/ereg607300.cfm?clear blocked::http://www.goeshow.com/nsh/VIR/ereg607300.cfm?clear VIR Teleconference Summer Series NSH teleconferences are a great, inexpensive way to provide continuing education to a large number of employees. The cost for each teleconference is the same regardless of the number of attendees. Topics include: * June 29th: Selection, Production and Characterization of Probes for In Situ Hybridization presented by Stephanie Villarreal, Merck * July 20th: Working Up a New Antibody in Animal Tissue Sections presented by Elizabeth A. Chlipala, Premier Laboratory, LLC * August 17th: Resin Histology: A Practical Approach for Demonstrating Undemineralized Bone presented by Jack Ratliff, BioMimetic Therapeutics, Inc. Registration Fee: $125.00 Per Teleconference: includes materials and sign in sheets, audio CD and test (good for contact hour credit for 2 years); Order the entire VIR Summer Series by June 28, 2011 and save $100.00! For abstracts and registration information: https://www.goeshow.com/nsh/VIRTeleconferences/ereg416386.cfm?pg=home blocked::https://www.goeshow.com/nsh/VIRTeleconferences/ereg416386.cfm? pg=home Any questions please contact the NSH Office, 443-535-4060. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT/HTL needed in St. Augustine, FL
A friend has asked me to post a request for an HT/HTL for a GI lab in St. Augustine, FL. For more information, please contact Robin Scott at rsc...@gi-associates.com. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] negative controls on immunos
If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HELP!!!!! H. Pylori Immunos
We usually run them on our automated stainers but I have been known to do them by hand in a pinch Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heather Cooper [hctrup...@att.net] Sent: Tuesday, March 01, 2011 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP! H. Pylori Immunos Does anyone do H. Pylori Immunos by hand? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: negative controls on immunos
Yes, you should run negative controls with each run - today and tomorrow. Something can happen in tomorrow's run so tomorrow's negative control will have different issues from today's negative control. The negative control should go through everything that your slides go through, whether it's today's great run or tomorrows electrical black out or whatever mishap. Whenever you run a test you must have a positive control and a negative control. Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ 07103 USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McMahon, Loralee A Sent: Tuesday, March 01, 2011 1:30 PM To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: negative controls on immunos We do not run additional negatives with each run on the same block. If it was a different block then yes we would run a negative. It takes up too many spaces on the machine and uses reagents. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig [dmcc...@ckha.on.ca] Sent: Tuesday, March 01, 2011 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls on immunos If you do a run of several immunos today and you run a negative control, and there is a request for additional immunos tomorrow, would you run another negative control with the additional slides. They are being stained on a stainer and not manually, diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: negative controls on immunos
I was also taught to run a positive and negative every time you do an immuno stain, provided there is sufficient tissue. I was trained at AFIP, by the people that wrote the second AFIP staining manual (the red one). Of course, if cost is your number one concern. Sincerely, Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How many tissues an histo tech is suppose to cut per hour?
30 blocks/hour, including rough cutting, is the standard. That's supposing a few (5-10) levels, specials, etc. If I am cutting all big tissue, what I've heard called onesies (just one slide each), I can cut 60 blocks/hour. If one is cutting a tray full of persnickety prostate (with 6 levels on one slide), renal, and bone marrow bxs with tons of specials and immunos, well, that is going to slow you down. Anyway, I disagree with those who say speed isn't as important as quality, patience, etc. I think new histotechs should have someone with a stopwatch and a clipboard standing behind them while they're cutting, yelling Faster, faster in their ear. If anyone cannot measure up to a strict quota they should be immediately dismissed. We have such a disgusting oversupply of new Histotechnology students as it is. ;) Jay A. Lundgren M.S., HTL(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histo tech is suppose to cut per hour?
I truly hope that was humor about oversupply. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, March 01, 2011 1:35 PM To: Mahoney,Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per hour? 30 blocks/hour, including rough cutting, is the standard. That's supposing a few (5-10) levels, specials, etc. If I am cutting all big tissue, what I've heard called onesies (just one slide each), I can cut 60 blocks/hour. If one is cutting a tray full of persnickety prostate (with 6 levels on one slide), renal, and bone marrow bxs with tons of specials and immunos, well, that is going to slow you down. Anyway, I disagree with those who say speed isn't as important as quality, patience, etc. I think new histotechs should have someone with a stopwatch and a clipboard standing behind them while they're cutting, yelling Faster, faster in their ear. If anyone cannot measure up to a strict quota they should be immediately dismissed. We have such a disgusting oversupply of new Histotechnology students as it is. ;) Jay A. Lundgren M.S., HTL(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histo tech is suppose to cut per hour?
I hope the whole paragraph was supposed to be humor or Jay may have a lynch mob at his door! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 01, 2011 3:00 PM To: 'Jay Lundgren'; Mahoney,Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per hour? I truly hope that was humor about oversupply. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, March 01, 2011 1:35 PM To: Mahoney,Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per hour? 30 blocks/hour, including rough cutting, is the standard. That's supposing a few (5-10) levels, specials, etc. If I am cutting all big tissue, what I've heard called onesies (just one slide each), I can cut 60 blocks/hour. If one is cutting a tray full of persnickety prostate (with 6 levels on one slide), renal, and bone marrow bxs with tons of specials and immunos, well, that is going to slow you down. Anyway, I disagree with those who say speed isn't as important as quality, patience, etc. I think new histotechs should have someone with a stopwatch and a clipboard standing behind them while they're cutting, yelling Faster, faster in their ear. If anyone cannot measure up to a strict quota they should be immediately dismissed. We have such a disgusting oversupply of new Histotechnology students as it is. ;) Jay A. Lundgren M.S., HTL(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histo tech is suppose to cut per hour?
Bet you he already does. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, March 01, 2011 3:01 PM To: 'Marcum, Pamela A'; 'Jay Lundgren'; Mahoney, Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per hour? I hope the whole paragraph was supposed to be humor or Jay may have a lynch mob at his door! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 01, 2011 3:00 PM To: 'Jay Lundgren'; Mahoney,Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per hour? I truly hope that was humor about oversupply. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren Sent: Tuesday, March 01, 2011 1:35 PM To: Mahoney,Janice A Cc: histonet@lists.utsouthwestern.edu; Jenny Vega Subject: Re: [Histonet] How many tissues an histo tech is suppose to cut per hour? 30 blocks/hour, including rough cutting, is the standard. That's supposing a few (5-10) levels, specials, etc. If I am cutting all big tissue, what I've heard called onesies (just one slide each), I can cut 60 blocks/hour. If one is cutting a tray full of persnickety prostate (with 6 levels on one slide), renal, and bone marrow bxs with tons of specials and immunos, well, that is going to slow you down. Anyway, I disagree with those who say speed isn't as important as quality, patience, etc. I think new histotechs should have someone with a stopwatch and a clipboard standing behind them while they're cutting, yelling Faster, faster in their ear. If anyone cannot measure up to a strict quota they should be immediately dismissed. We have such a disgusting oversupply of new Histotechnology students as it is. ;) Jay A. Lundgren M.S., HTL(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HELP!!!!! H. Pylori Immunos
Our problem, in my opinion is a control issue and not a staining one..our pathologist wants to see the bugs in certain areas-glands(spelling appologies!) its called, the circular groups of cells, in the middle of these areas and not just occasional bugs in other areasEverywhere else I've worked the pathologist would use a Diff-Quick, Cresyl Violet or Geimsa in addition to the HE, I'm aware of other newer special stains for H.Pylori that are out there, but this pathologist is firm on the IHC method of choice. I use NovaCastra polyclonal, incubation @23 min. HIER with low PH for 30 minutes.with recommended detection kit.. Heather, I hope you don't mind that I copied your email so that everyone on this forum can learn from your question. I always use the Reply to All button so everyone can see my response. I was willing to bet before I read your query that we were dealing with a control problem, not a staining problem. I have seen some miserable commercial *H. pylori* controls lately. Some areas of the country are awash in HP, some labs can't get a good positive control block of their own. You must first obtain a KNOWN POSITIVE CONTROL block. Stain slides from that block with whichever chemical special stain is acceptable to your Pathologist, I like a modified Warthin-Starry. Sit down with your Pathologist and evaluate the control slides. Is it acceptable to the Pathologist? If so, stain more slides from the positive control block, this time immuno stain and chemical special stain in parallel. Evaluate the slides with the Pathologist. Is there correlation between the chemical and special stain? If so, proceed, running your first 20 cases in parallel (immuno and chemical special stains) and documenting (with the Pathologist's signature) the correlation of your first 20 cases. If you are able to demonstrate HP with a chemical special stain, but not with your immuno procedure, call your immuno vendor. If you are unable to demonstrate HP with a chemical special stain, it's your control. Or it could be your stain, but a Giemsa is pretty hard to mess up. Good Luck, Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How many tissues an histo tech is suppose to cut per hour?
;) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histo tech is suppose to cut per hour?
Humor on Histonet... nevah - as my youngest grandson says about everything:) Let's have more.. Report on GMA this morning said humor and good attitude makes us healthier! J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How many tissues an histotech is suppose to cut per hour?
Hello everybody. Thanks for your replies. I did not expected so many answers to my questions. Well today I talked to my supervisor about ways I could improve the speed of my sectioning, and she and another co-worker pointed out a couple of things that I have been doing that were wasting a lot of time. To me they were little things but I stopped doing them today and I was able to finish half- an hour earlier than I am used to. I still need to improve the speed and cut more blocs per hour. I was able to cut about 20-25 an hour but I want to be able to cut more (30 and more)without sacrificing the quality of the blocks. If I don't see the complete tissue on the slide I go crazy. We'll take care. Thanks. On Mon, Feb 28, 2011 at 8:58 PM, Sheila Adey sa...@hotmail.ca wrote: Hi Jenny, This is so dependant on what type of tissue you are cutting and if you are picking up extra levels or spares. Also using an automated microtome can be much faster. Good luck to you. Date: Mon, 28 Feb 2011 18:18:37 -0400 From: histotech...@gmail.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine he slides) in the microtome. I am a new histotech and I would like to know how much tissue do an experienced histotech cuts per hour. For example if you have 100 tissues per total in how many hours are you suppose to cut this tissue? or.tell me how much you cut at your lab. Do you have any suggestions on how to cut faster? Thank You. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IF controls
Tonsil controls for C3,C1q,IgA,IgG,IgM,Kappa,Lambda, and Fibrinogen. Used placenta to verify Fibrinogen also. Roszetta Butler, HT(ASCP),IF tech Ameripath, 3000 United Founders Blvd, Suite 135, Oklahoma City, OK 73112, phone (405)841-7875, fax (405)841-2002, email rbut...@ameripath.com Please think about resource conservation before you print this message CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, March 01, 2011 12:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 88, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: How many tissues an histotech is suppose to cut per hour? (Podawiltz, Thomas) 2. RE: How many tissues an histo tech is suppose to cut per hour? (Mahoney,Janice A) 3. RE: How many tissues an histotech is suppose to cut per hour? (Marcum, Pamela A) 4. RE: How many tissues an histotech is suppose to cut per hour? (Mahoney,Janice A) 5. looking for KJ Kellogg (Cheri Miller) 6. Slide filing system search (Bartlett, Jeanine (CDC/OID/NCEZID)) 7. Labelers - Needs and Must Haves (Tonia Richmond) 8. RE: Labelers - Needs and Must Haves (Laurie Colbert) 9. Re: How many tissues an histotech is suppose to cut per hour? (Rene J Buesa) 10. I.F. Controls (hardwi...@techie.com) 11. RE: Labelers - Needs and Must Haves (WILLIAM DESALVO) 12. HELP! H. Pylori Immunos (Heather Cooper) -- Message: 1 Date: Tue, 1 Mar 2011 09:26:25 -0500 From: Podawiltz, Thomas tpodawi...@lrgh.org Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? To: 'Konop, Nicole' nko...@chw.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, 'Jenny Vega' histotech...@gmail.com Message-ID: 38667e7fb77ecd4e91bfaeb8d986386323de133...@lrghexvs1.practice.lrgh.org Content-Type: text/plain; charset=us-ascii Worry more about quality than speed at this time. I measure my staff on quality rather than how fast things get done. It's a waste of time, energy and resources to have to re-cut blocks because of poor quality. Speed comes with time and experience. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Konop, Nicole Sent: Tuesday, March 01, 2011 9:14 AM To: histonet@lists.utsouthwestern.edu; 'Jenny Vega' Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? I agree with Cynthia. Practice and setting up a standard system of how you cut. I do the same thing every time with each block I cut. Cutting faster is not necessarily a good thing if you are jeopardizing quality just to cut more slides. It's quality over quantity in my book any day! Nicole Anne Konop BS, HTL(ASCP) Histology Team Lead Children's Hospital of Wisconsin (414)266-6580 Direct Line (414)907-0366 Pager (414)266-2524 Histology Department -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Tuesday, March 01, 2011 7:51 AM To: 'Jenny Vega'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histotech is suppose to cut per hour? Practice, practice, practice! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Monday, February 28, 2011 5:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histotech is suppose to cut per hour? I would like to know how many tissues does a experienced histotech is suppose to cut per hour (routine he slides) in the microtome. I am a new histotech and I would like to know how much tissue
RE: [Histonet] HELP!!!!! H. Pylori Immunos
We also cut our controls in sequence. Stain the first of the sequence and the last. To make sure that you have not cut through the area. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: Jay Lundgren [jaylundg...@gmail.com] Sent: Tuesday, March 01, 2011 3:10 PM To: McMahon, Loralee A Cc: Heather Cooper; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] HELP! H. Pylori Immunos Our problem, in my opinion is a control issue and not a staining one..our pathologist wants to see the bugs in certain areas-glands(spelling appologies!) its called, the circular groups of cells, in the middle of these areas and not just occasional bugs in other areasEverywhere else I've worked the pathologist would use a Diff-Quick, Cresyl Violet or Geimsa in addition to the HE, I'm aware of other newer special stains for H.Pylori that are out there, but this pathologist is firm on the IHC method of choice. I use NovaCastra polyclonal, incubation @23 min. HIER with low PH for 30 minutes.with recommended detection kit.. Heather, I hope you don't mind that I copied your email so that everyone on this forum can learn from your question. I always use the Reply to All button so everyone can see my response. I was willing to bet before I read your query that we were dealing with a control problem, not a staining problem. I have seen some miserable commercial H. pylori controls lately. Some areas of the country are awash in HP, some labs can't get a good positive control block of their own. You must first obtain a KNOWN POSITIVE CONTROL block. Stain slides from that block with whichever chemical special stain is acceptable to your Pathologist, I like a modified Warthin-Starry. Sit down with your Pathologist and evaluate the control slides. Is it acceptable to the Pathologist? If so, stain more slides from the positive control block, this time immuno stain and chemical special stain in parallel. Evaluate the slides with the Pathologist. Is there correlation between the chemical and special stain? If so, proceed, running your first 20 cases in parallel (immuno and chemical special stains) and documenting (with the Pathologist's signature) the correlation of your first 20 cases. If you are able to demonstrate HP with a chemical special stain, but not with your immuno procedure, call your immuno vendor. If you are unable to demonstrate HP with a chemical special stain, it's your control. Or it could be your stain, but a Giemsa is pretty hard to mess up. Good Luck, Jay A. Lundgren M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Traveling Histotechs
Does anyone have information on any agencies that specialize in positions for traveling histotechs? Thank you, Jennifer MacDonald ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
