[Histonet] ExCell Plus fixative
Hello Histo Land, Does anyone out there have experience using ExCell Plus formalin substitute? Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] barcoding..
Anyone using TBS's SHUR/Mark® Software? Looking for references, what works, downtime, what would you change if you could? Thanks! Rosa Fields, HT (ASCP) Gastroenterology Specialties Histology Supervisor 4545 R Street Lincoln, NE 68503 402-465-4545 rfie...@gidocs.net The information contained in the message and the documents accompanying this message contain information that is privileged and confidential and is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication, other than its return to the sender, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] diastase
I am looking to purchase diastase to use for the PAS/DIASTASE stain. I see there are many options in the catalogues. What is recommended? diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] diastase
I have a long distance on-line histology student in my lab right now getting her practical experience. She was doing special stains this past week and had PAS with and without diastase. I had her do the stains with both commercially available diastase and also using saliva so she could see the difference. Nothing like the stains we use to do in the Good Ol' Days! What a difference. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, March 09, 2011 8:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] diastase I am looking to purchase diastase to use for the PAS/DIASTASE stain. I see there are many options in the catalogues. What is recommended? diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: cell blocks
I actually fixed the cells without the agar overnight, and left them in the agar overnight... Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: Tony Henwood [mailto:antho...@chw.edu.au] Sent: Tuesday, March 08, 2011 6:43 PM To: Sarah Goebel; histonet@lists.utsouthwestern.edu Subject: RE: cell blocks Sarah, It is important that the agar cell block is allowed to fix in formalin (10%NBF) before wrapping in paper, at least 2 hours. It will hold its shape better. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: Wednesday, 9 March 2011 2:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cell blocks So...I am trying to make a cell block using 0.9% agar. I have done this in the past with no problems. It was at a different facility and I don't know if the agar is the same? I used bacto-agar this time, but last week someone tried to do my protocol with agarose and the boogers dried up and fell out of the block. The only other difference is we have clear rite xylene substitute in this processor where the other one I used had xylene. Tissues processed on the same cycle come out fine with the clear rite so I don't think that it the problem. When the cell blocks come out they are as thin as the papers I wrapped them in making it almost impossible to see and to subsequently cut. Any suggestions for making cell blocks without using the plasma/thrombin method or the insanely expensive histo-gel would be appreciated. Second, does anyone have a suggestion on how to repair the cells that are embedded in the tissue paper thick cell block? Thanks guys and gals! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] diastase
Just hack up some spit... Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, March 09, 2011 8:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] diastase I am looking to purchase diastase to use for the PAS/DIASTASE stain. I see there are many options in the catalogues. What is recommended? diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Extended processing for breasts
Would someone who has extended their processing times to accomodate the CAP regs for breast tissue be willing to share their processing schedules with me? Up until now, we've had someone in over the weekend to add breast tissue to processor but are having staffing issues so I'm looking for alternatives. Thanks a bunch! Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Extended processing for breasts
Generally the easiest thing to do is to extend your time in the 70% ETOH or other low percentage ETOH. This allows you to have a cut off on the weekend vs Friday. We had times of 24 to 48 hours depending upon weekend holiday rotations etc... Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas [micropathl...@yahoo.com] Sent: Wednesday, March 09, 2011 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Extended processing for breasts Would someone who has extended their processing times to accomodate the CAP regs for breast tissue be willing to share their processing schedules with me? Up until now, we've had someone in over the weekend to add breast tissue to processor but are having staffing issues so I'm looking for alternatives. Thanks a bunch! Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE:FORMALIN CONTAINERS
Hello, Does anyone clean and re-use large specimen containers? If so, how do you prepare the containers for cleaning to ensure the cleaning staff are not exposed to residual formalin? Thanks Sue The information contained in this e-mail and document(s) attached are for the exclusive use of the addressee and may contain confidential, privileged and non-disclosable information. If the recipient of this e-mail is not the addressee, such recipient is strictly prohibited from reading, photocopying, distributing or otherwise using this e-mail or its content in any way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 88, Issue 11
Merci pour votre message. Je suis actuellement absent du laboratoire. Je repondrai a votre message a mon retour a partir du 15 mars 2011. En cas d'urgence merci de contacter : Sylvie Tambutté (stambu...@centrescientifique.mc) - +377 93 30 12 11. Thank you for your email. I am currently out of the lab. I will reply to your message after the 15th of march 2011. For any problem, contact Dr Sylvie Tambutté (stambu...@centrescientifique.mc). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pulling apart sections
We use a teasing needle to pull apart our section. Jusith Pardue Memorial Health Care System Chattanooga, Tn. 37404 This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. Sections 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: RE:FORMALIN CONTAINERS
We do not re-use large specimen containers. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Seguin, Suzanne Sent: Wednesday, March 09, 2011 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE:FORMALIN CONTAINERS Hello, Does anyone clean and re-use large specimen containers? If so, how do you prepare the containers for cleaning to ensure the cleaning staff are not exposed to residual formalin? Thanks Sue The information contained in this e-mail and document(s) attached are for the exclusive use of the addressee and may contain confidential, privileged and non-disclosable information. If the recipient of this e-mail is not the addressee, such recipient is strictly prohibited from reading, photocopying, distributing or otherwise using this e-mail or its content in any way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] band saws
You don't need a large powerful band saw to cut bone. Bone is quite soft compared to the kinds of substances, particularly metals, which those larger band saws can handle. I bought one for my lab over 12 years ago, the smallest, cheapest Craftsman model Sears carried, and it's still going strong. I use it for bone work and also for trimming methyl methacrylate blocks. The most important thing is the type of blade you use. Blades designed for general cutting of wood are too coarse (the teeth are too large) for bone work. A plywood cutting blade might be ok, but I find that blades designed for cutting metal are best. The teeth are much finer (I use blades with 14 teeth per inch), and they have the added advantage of staying sharp a lot longer than a wood cutting blade. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Pulling apart sections
I use very fine-pointed curved forceps Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pardue, Judith Sent: Wednesday, March 09, 2011 1:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pulling apart sections We use a teasing needle to pull apart our section. Jusith Pardue Memorial Health Care System Chattanooga, Tn. 37404 This message and accompanying documents are covered by the Electronic Communications Privacy Act 18 U.S.C. Sections 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CMV
I am looking for a CMV antibody that is IVD. I am hoping to find a good vendor that distributes it. Thanks! Mary Ring, HT, QIHC Regions Hospital 640 Jackson St St Paul, Mn 55101 mary.l.r...@healthpartners.com This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] DAKO Artisan Kit Issues
We periodically have plunger and leaking issues.they always replace the kits. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mizly...@frontier.com Sent: Monday, March 07, 2011 2:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAKO Artisan Kit Issues Fellow Histologists: This is my first time using Histonet but I need to ask if anyone has started having problems with their DAKO Artisan kits recently? We have started having leaking kits for Gram Lot # 10043062 and Expiration Date 4/30/2011, and plunger issues with the Iron Lot # 10047616 Expiration Date 10/31/2011. We have also found that the GMS kits Lot # 10049354 and Expiration 11/30/2011 we have received in the last two shipments are from the same lot but they do not work with our validated protocol. What is strange is that the previous GMS kit worked on the same control that we are using now, but does not work with the new kit, but when stained with PAS-Fungus we see the same fungus as we saw with the previous GMS kit. I think it could be something with the shipping method but wanted to see if anyone else has seen a difference. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] barcode scanner for tissue cassette
Can anyone recommend a suitable barcode scanner that will work with the ThermoFisher Microwriter? I have 5 units that are currently using either a Symbol or a Cognex scanner that do not function appropriately. Requires multiple passes (4-6 if at all) of the 2D labeled cassette in order for the slide to print. Very seldom does it print on the 1st pass. When you are dealing with 500 - 700 cassettes there is no time for multiple passes. I don't know if it is the scanners, software or just settings. I have not had much success from the company regarding updating the scanners to help resolve this issue. John Ryan Assistant Administrative Director Pathology 832-355-2643 phone 832-355-4232 fax jr...@sleh.commailto:jr...@sleh.com +CONFIDENTIALITY NOTICE+ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] barcode scanner for tissue cassette
John, have your tried pastel colored cassettes? Scanning works much better with those. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ryan, John P. Sent: Wednesday, March 09, 2011 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] barcode scanner for tissue cassette Can anyone recommend a suitable barcode scanner that will work with the ThermoFisher Microwriter? I have 5 units that are currently using either a Symbol or a Cognex scanner that do not function appropriately. Requires multiple passes (4-6 if at all) of the 2D labeled cassette in order for the slide to print. Very seldom does it print on the 1st pass. When you are dealing with 500 - 700 cassettes there is no time for multiple passes. I don't know if it is the scanners, software or just settings. I have not had much success from the company regarding updating the scanners to help resolve this issue. John Ryan Assistant Administrative Director Pathology 832-355-2643 phone 832-355-4232 fax jr...@sleh.commailto:jr...@sleh.com +CONFIDENTIALITY NOTICE+ The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Control Slides
Is there guidelines for how long special stains controls are good for once they are cut? We have spirochetes for our Steiner that is from 2007 and we are having issues. Amanda Amador, HT(ASCP)CM AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A | Indianapolis, IN 46219 | phone 317.275.8052 | aama...@ameripath.commailto:aama...@ameripath.com | www.AmeriPath.comhttp://www.ameripath.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ArrayMold System
Hi, we have Arraymold more than a year now and we prepared around 10 TM's with it, it is really simple, easy and effective, however we found instructions to keep contructed TM overnight on 37 degrees uneffective since recipient and donor paraffin were not blend enough, we keep our TM's overnight on 40 degrees and next day half an hour on 60 (in moulds) if necessary we even prolonge this phase, this also help us to achieve that shorter tissue bars come down to the level. regards Irena Kirbis Date: Tue, 8 Mar 2011 10:59:46 -0500 From: alietee_langsd...@dfci.harvard.edu To: histonet@lists.utsouthwestern.edu Subject: re: [Histonet] ArrayMold System Hi, I am also interested in hearing about the ArrayMold vs Beecher vs any other do-it-yourself TMA systems. Any favorites systems/recommendations or suggestions? Thanks ~Ally Senior Research Technician Medical Oncology Dana-Farber Cancer Institute re: Wondering if anyone has used/purchased the ArrayMold tissue micro array system?? Positive and negative feedback would be greatly appreciated :) Nancy Heath, HT(ASCP) March 10, 2011 is Histotechnology Professionals Day Senior Research Technician Medical Oncology Dana-Farber Cancer Institute The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
