[Histonet] g ratio
Hello, We have to measure the g ratio (ratio of the inner axonal diameter to the total outer diameter) - mice sciatic nerve - resin sectioning / toluidine blue - at least 500 myelinated fibers per nerve will be evaluated with a standard imaging software. Do you think that we can measure the number of demyelinated axons without perform any additionnal stainings? Thank you, Michel Michel BATAILLON Histology Evreux, France * Ce message et toutes les pieces jointes sont confidentiels et etablis a l'attention exclusive de ses destinataires. Si vous recevez ce message par erreur, merci de le detruire et d'en avertir immediatement l'expediteur. Toute utilisation de ce message non conforme a sa destination, toute diffusion ou toute publication, totale ou partielle, est interdite. Internet ne permettant pas de garantir l'integrite de ce message, le CIT decline toute responsabilite au titre de ce message s'il a ete altere, deforme ou falsifie. Protegeons ensemble l'environnement, n'imprimons ce mail (et ses pieces jointes) que si cela est necessaire. This transmission and any attachments are confidential and intended solely for the use of the addressee(s). If you are not an intended recipient, please notify us immediately by replying to the message and deleting it from your computer. In this case, any unauthorized use, copying or distribution is strictly prohibited. Please be aware that E-mails are susceptible to alteration and their integrity cannot be guaranteed. CIT shall not be liable for this E-mail if corrupted, changed or falsified. Please consider the environment before printing this E-mail. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 88, Issue 16
Here is the latest document from the UK on productivity. http://system.improvement.nhs.uk/ImprovementSystem/ViewDocument.aspx?path=Diagnostics%2fNational%2fWebsite%2fHistology%20Guide%202.pdf _ David Muskett Chief Biomedical Scientist, Histology East Lancashire Hospitals NHS Trust | Royal Blackburn Hospital | Haslingden Road, Blackburn | BB2 3HH david.musk...@elht.nhs.uk | www.pathologyineastlancs.org.uk Telephone x 82438 | 01254 732438 | Fax 01254 736081 |Histology results 01254 732621 or internal x82621 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: 13 March 2011 17:02 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 88, Issue 16 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. bone decal (Thomas Huynh) 2. slides to pathologist - one response. (Cheryl) 3. Competency Checklist (Damaris Beil) -- Message: 1 Date: Sat, 12 Mar 2011 10:51:30 -0800 (PST) From: Thomas Huynh thomas6...@yahoo.com Subject: [Histonet] bone decal To: histonet@lists.utsouthwestern.edu Message-ID: 327910.66086...@web80405.mail.mud.yahoo.com Content-Type: text/plain; charset=iso-8859-1 Hi Mary We use 10% Formic Acid for our bone marrow bx and larger surgical bones (Ex: femur, pel-vis, mandibular bones) Thomas Huynh ? From: histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Sat, March 12, 2011 12:01:57 PM Subject: Histonet Digest, Vol 88, Issue 15 Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: ? 1. equine bone decal (Mary Lou Norman) ? 2. FW: Slides to Pathologist (Weems, Joyce) ? 3. Leica TP1020 (Lucy Zong) ? 4. RE: Slides to Pathologist (Nails, Felton) ? 5. (no subject) (Katrena Fraka) ? 6. temp needed asap (Cheryl) -- Message: 1 Date: Fri, 11 Mar 2011 15:19:10 -0500 From: Mary Lou Norman ml...@cornell.edu Subject: [Histonet] equine bone decal To: histonet@lists.utsouthwestern.edu ??? histonet@lists.utsouthwestern.edu Message-ID: ??? 4010584de26d90419367c0a5f620e9633027f1c...@mbxb.exchange.cornell.edu Content-Type: text/plain; charset=us-ascii Hi, We've been using EDTA to decal and of course it takes forever. I would love to hear what others are using for large bones, joints, etc. We do not work on rats or mice, just horses. Thanks. Many prayers to Japan and beyond. Mary Lou -- Message: 2 Date: Fri, 11 Mar 2011 16:39:04 -0500 From: Weems, Joyce jwe...@sjha.org Subject: [Histonet] FW: Slides to Pathologist To: Histonet histonet@lists.utsouthwestern.edu Message-ID: ??? 92ad9b20a6c38c4587a9febe3a30e164081e21a...@chexcms10.one.ads.che.org Content-Type: text/plain; charset=us-ascii I received this question from my Quality Manager today. Hi Joyce, What do you consider good performance regarding this?? I need some sort of goal for our lab key control points chart. I could only find one reference on the internet from Presbyterian Hospital in Charlotte, NC from 1993 (yuck).? Their goal was 90% of the slides on time which they correlated to 2 days or less late slides per month. Their goal was also to have all the slides out by 11am.. Could I get feedback from you guys about what you expect for this PI goal? I'd like 100%, but sometimes staffing just doesn't stretch far enough!! Thanks, Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is
[Histonet] Daylight Savings Time?
Does anyone know the OLD daylight savings time date that USED to be in effect? I'll have to manually change the processor time to avoid being late (or early - who knows?). Today, I was way early! Senior moment, I suppose... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Daylight Savings Time?
I think it used to be the first weekend in April,but I was also told it may have been this coming weekend (in Europe anyway) Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, March 14, 2011 8:00 AM To: Histonet Subject: [Histonet] Daylight Savings Time? Does anyone know the OLD daylight savings time date that USED to be in effect? I'll have to manually change the processor time to avoid being late (or early - who knows?). Today, I was way early! Senior moment, I suppose... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New York State Histotechnology Annual Spring Symposium Program
NYSHS ANNUAL SPRING SYMPOSIUM “SLIDING INTO THE FUTURE” May 14, 2011 Desmond Hotel and Conference Center Albany, NY The NYSHS has planned a wonderful program for this spring allowing its members to receive 6 CEU’s of credit in one day! You will be receiving your programs in the mail very soon but we would like to give you the meeting overview for those that need to submit for funding. Registration opens at 7:00am and the first session is at 8:00am. Program and registration will be posted on the NYSHS website www.nyhisto.org ( http://www.nyhisto.org/ )soon. 1) Virgil Hernadez CT (ASCP) Title: Digital Pathology Specialist, Ventana Medical Systems Talk Title: Digital Pathology 101 Abstract:This course will introduce meeting participants to the exciting new world of Digital Pathology. This 1 hour presentation will provide broad overview of basic system hardware and software required for converting prepared slides to whole slide images. Participants will become familiar with key applications of Digital Pathology which include: Telepathology, teleconsultation, web conferencing, IHC analysis, tumor board, and robotic microscopy. Current market trends in Digital Pathology adoption and products will be discussed. 2) Dr. Kari Reiber Title: Chief Medical Examiner, Dutchess County, NY. Dutchess County Department of Health Talk Title: The role of Histopathology in Forensic Postmortem Investigations Abstract: The ever increasing popularity of crime fiction has done little to improve the public’s understanding of forensic pathology. Fictional dramatizations focus on the forensic sciences rather than on forensic pathology, and often confuse the two. The popularity of “forensics” is having a positive effect, in that many young people are opting for a career in science. Unfortunately the so-called “CSI effect” is having a negative impact in the courtroom as a result of the unrealistic expectations of some jury members. Fictional medical examiners have many unrealistic identities and are portrayed as gun-toting vigilantes, forensic technology wizards, glamorous law enforcement officers, or cranky eccentrics, but almost never with their one essential instrument: the microscope. One forgets that forensic pathologists are actually pathologists specialized in the anatomy of injury and injury patterns. When investigating sudden, unexplained, and violent deaths, the forensic pathologist is mandated by law to perform a postmortem examination which generally consists of an autopsy. A complete forensic autopsy usually requires an external examination, an internal examination, a microscopic examination, and a comprehensive toxicological analysis. The purpose of this presentation is to define forensic pathology and forensic science, to clarify the actual role of the forensic pathologist and to illustrate by way of specific examples the crucial role of histopathology in forensic postmortem investigations. 3) Joseph Dudek, M.D. , US Oncology Incorporated – New York Oncology Hematology. Talk Title: Personalized Approach for Non-Small Cell Lung Cancer Abstract: The treatment of non-small cell lung cancer will be reviewed and a discussion of how systemic treatment is determined based on the histology and stage of the tumor. The importance of adenocarcinoma, squamous cell carcinoma and NOS will be discussed in regard to first line and second line systemic treatments. There are definite differences in regard to the choice of chemotherapy and its effectiveness in squamous and non-squamous histologies. We will also discuss EGFR mutations and there can influence on choice of systemic therapy for non-small cell lung cancer. Tyrosine kinase Inhibitors provide an alternative systemic treatment for patients with EGFR mutations. Toxicities of the treatment will be reviewed. Lastly the EML4- ALK mutation will be reviewed and its influence on potential treatments will be discussed. 4) Valantou Grover, HT, HTL(ASCP), PA, MBA Title: Biosciences Product Line Manager, Polysciences, Inc. Talk Title: The Right Stain, Troubleshooting Histology Stains Abstract: When routine stains go wrong, pathologists return slides to the responsible department for restaining: histology (routine and special stains), cytology or hematology. The repeat staining process on the old and/or the new slides reduces the expected turnaround time. Processes exist far beyond the control of the technician/technologist, not related to their skill, technique, and/or experience. Inconsistent staining may occur because townships change additives in water supply systems or filtration processes, mistakes in manufacturing processes as simple as water temperature variance, market supply, market demand, quality of the raw materials, availability of raw materials, incorrect shipping department standards, and/or the environment. The presentation allows lab professionals to examine troubleshooting techniques considered “outside the box” or scope of what is
RE: [Histonet] Daylight Savings Time?
Yes, it used to be April 2 (in the U.S.). I had to check an older calendar to be sure: http://www.timeanddate.com/time/dst/2000a.html --On Monday, March 14, 2011 11:28 AM -0500 Bernice Frederick b-freder...@northwestern.edu wrote: I think it used to be the first weekend in April,but I was also told it may have been this coming weekend (in Europe anyway) Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Monday, March 14, 2011 8:00 AM To: Histonet Subject: [Histonet] Daylight Savings Time? Does anyone know the OLD daylight savings time date that USED to be in effect? I'll have to manually change the processor time to avoid being late (or early - who knows?). Today, I was way early! Senior moment, I suppose... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician III Cardiovascular Medicine 348 Biomedical Research Building State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 USA lei...@buffalo.edu 716-829-6118 (Ph) 716-829-2665 (Fx) No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Opening
We are still looking for a certified histotech to run a new in-office GI path lab in Santa Rosa, CA. Excellent pay and working conditions. Must have experience. Hours are very flexible. No new grads (sorry). Send resume to tja...@yahoo.com Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] VALIDATION of a new clone
I am wondering what others are doing when they validate a new clone of a antibody that you have used in the lab for quite sometime. When we introduce a new antibody obviously we go through a pretty extensive validation process including sampling of around 20 - 30 specimens, some presumed positive cases and some presumed negative cases. We also try to include in the positive cases some that are strongly positive and others that are weakly positive. Lately several of our regular antibodies are now no longer available in the same clone. I am interested in how different labs would validate the new clone to be used in the lab. It would seem to me that the validation process would not have to be as extensive since we have already established that the antibody works well in our laboratory. I know if it was the same clone but a different lot we would just do a comparison of the old lot with the new lot, but what about a different clone? I realize that the methods are going to be diverse but I would appreciate hearing from others. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] VALIDATION of a new clone
If it's a different clone then it's essentially a different antibody you would have to go through the same process as you did initially. LIz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, March 14, 2011 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VALIDATION of a new clone I am wondering what others are doing when they validate a new clone of a antibody that you have used in the lab for quite sometime. When we introduce a new antibody obviously we go through a pretty extensive validation process including sampling of around 20 - 30 specimens, some presumed positive cases and some presumed negative cases. We also try to include in the positive cases some that are strongly positive and others that are weakly positive. Lately several of our regular antibodies are now no longer available in the same clone. I am interested in how different labs would validate the new clone to be used in the lab. It would seem to me that the validation process would not have to be as extensive since we have already established that the antibody works well in our laboratory. I know if it was the same clone but a different lot we would just do a comparison of the old lot with the new lot, but what about a different clone? I realize that the methods are going to be diverse but I would appreciate hearing from others. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] VALIDATION of a new clone
Hi Jim, If it's a new clone then a new valiation should be performed. I know of many instances where one clone stains something that another doesn't. If this is regarding Ventana not selling certains clones, those clones are still available from Cell Marque directly. Mark On Mon, Mar 14, 2011 at 11:16 AM, Vickroy, Jim vickroy@mhsil.comwrote: I am wondering what others are doing when they validate a new clone of a antibody that you have used in the lab for quite sometime. When we introduce a new antibody obviously we go through a pretty extensive validation process including sampling of around 20 - 30 specimens, some presumed positive cases and some presumed negative cases. We also try to include in the positive cases some that are strongly positive and others that are weakly positive. Lately several of our regular antibodies are now no longer available in the same clone. I am interested in how different labs would validate the new clone to be used in the lab. It would seem to me that the validation process would not have to be as extensive since we have already established that the antibody works well in our laboratory. I know if it was the same clone but a different lot we would just do a comparison of the old lot with the new lot, but what about a different clone? I realize that the methods are going to be diverse but I would appreciate hearing from others. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Adequacy charges
Can we charge adequacy charges for each pass on an FNA. We enter each specimen separately and make cytospins on the rest of the material, and generate a report for each. We charge each case an 88108 for the cytospins. But are we allowed to charge an adequacy charge on each case. Also can the pathologist charge for each adequacy pass. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] g ratio
Bonjour, I think a Luxol Fast Blue is the standard for visualizing the myelin sheath. Here's a free tip; leave your slides in the LFB stain overnight, instead of the shorter time specified in most protocols. You're going to decolorize them anyway. Decolorize the slides until you don't see the stain run off anymore. You get a really brilliant LFB this way. The Pathologists I've worked with love it. Comment dit on myelin sheath en Francaise? Vive l'esprit de Lafayette! Jay A. Lundgren M.S., HTL(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Control Slides
We dry the control slide at the same time that we dry the tissue being stained, since controls are supposed to be handled in the same manner as the test tissue. That being said, I guess if we were truly following that rule, we would cut the control on the same day we cut the test sample. :-) Other people say that, after they dry the slide, they dip the slide in paraffin, to cover the tissue, so that the air doesn't touch the tissue during the months before the slide is used in a stain. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Thursday, March 10, 2011 6:39 PM To: Amador, Amanda; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Control Slides I've noticed that our spirochete control slides don't stain as intense starting about 3 months after they are sectioned, and that they stop staining by about 6 months. (This is with a silver stain.) I've talked with other histotechs, and they say they've seen the same phenomenon with AFB and Gram controls. (We use ours up too quickly - we never have 6-12 months old control slides for these, so I can't attest to this. ) Bancroft's book talks about this phenomenon with amyloid, and suggests that oxidation of the tissue (proteins) due to exposure to air may be the cause. I'm guessing the it probably applies to the precut microorganism control slides, too. We only cut enough slides for 6 months, and date them. Other people say they put their cut control slides in a slide box with a lid after drying, and then place the box in the refrig, as cold slows down the chemical change, and the lid keeps the moving air off the slide. Other people say that, after they dry the slide, they dip the slide in paraffin, to cover the tissue, so that the air doesn't touch the tissue during the months before the slide is used in a stain. Just 3 suggestions to stop this problem. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -- From: Amador, Amanda aama...@ameripath.com Sent: Wednesday, March 09, 2011 10:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Control Slides Is there guidelines for how long special stains controls are good for once they are cut? We have spirochetes for our Steiner that is from 2007 and we are having issues. Amanda Amador, HT(ASCP)CM AmeriPath | Histology Group Lead/Trainer |2560 N Shadeland Ave, Suite A | Indianapolis, IN 46219 | phone 317.275.8052 | aama...@ameripath.commailto:aama...@ameripath.com | www.AmeriPath.comhttp://www.ameripath.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] immunostaining question
Hello everybody, I am trying some new antibodies on parafin embeded radical prostactectomies tissue sections and I started with the highest recomended dilution but it seems I did not get any staining. I already counterstained the slides w/ hematoxylin and I was wondering if it's possible to go a few steps back and incubate again with a higher concentration of the antibody? I have a limited amount of tissue sections for testing. Any imput is appreciated! Thanks, Leni ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] immunostaining question
Yes you can, especially if the antigen is not nuclear. René J. --- On Mon, 3/14/11, Pop Elena med_l...@yahoo.com wrote: From: Pop Elena med_l...@yahoo.com Subject: [Histonet] immunostaining question To: histonet@lists.utsouthwestern.edu Date: Monday, March 14, 2011, 4:57 PM Hello everybody, I am trying some new antibodies on parafin embeded radical prostactectomies tissue sections and I started with the highest recomended dilution but it seems I did not get any staining. I already counterstained the slides w/ hematoxylin and I was wondering if it's possible to go a few steps back and incubate again with a higher concentration of the antibody? I have a limited amount of tissue sections for testing. Any imput is appreciated! Thanks, Leni ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Research and Clinical Labs
Hi, Let me preface this by stating that I don't think this is a good idea and it is not what we are doing here. I have a hypothetical question though. What would be the regulatory ramifications of merging a research lab testing animal specimens (not regulated by any agencies other than Best Practices and OSHA) with a traditional (CAP, JACHO) clinical histology lab. What equipment, if any, could be shared? In the event of a worst case scenario (like an inspector noticing mouse tissue and human tissue on the same processor at the same time or expired chemicals not labeled research use only) which regulations would be violated and how would one find out potential fines that could be incurred. I have heard that there are labs that are doing this. What precautions should a lab take if they end up venturing down this path. Thanks, Amos ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
