[Histonet] Storing slides in buffer
Hi guys, I am wondering if anyone has much experience storing slides in buffer (PBS-T) after retrieval for up to about 48hrs at 4C? Is it likely that they will still stain? Or is it going to be entirely dependent on the epitope? I'm optimizing research antibodies right now and I'd like to decrease my turn around time by knocking off the HIER at an earlier time point. Thanks. -- Stacy Deppeler. Research Assistant Department of Ophthalmology King Abdulaziz University Hospital Riyadh, KSA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] staining of damaged mitochondria
Hi, Do you know a way to stain damaged mitochondria in rat hearts after ischemia/reperfusion by immunohistochemistry? Thank you very much! Felix -- Felix Nagel Ludwig Boltzmann Cluster for Cardiovascular Research c/o Core Unit for Biomedical Research Waehringer Guertel 18-20 - Leitstelle 1Q A-1090 Vienna Austria Tel: +43 1 40400 5223 Mail: felix.na...@meduniwien.ac.at Website: www.cardiovascular-research.at ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Light hematoxylin staining in HE
Are you soaking your blocks in dilute ammonium hydroxide before sectioning? Soaking too long will affect stain quality. Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PEC1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 I find that the harder I work, the more luck I seem to have. - Thomas Jefferson Jordan, Velma velma.jor...@gwu-hospital.com 03/15/11 22:22 PM We have been troubleshooting complaints of hematoxylin staining light in our HE. The main complaint is no differentiation between the nucleus and cytoplasm. We are using Richard-Allan hematoxylin ,eosin,clarifier and bluing. We tried surgipath, changing times, pH the H2O and any other thing we could think of, but the problem remains. Does anyone have a suggestion? Velma Jordan UHS Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient (s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Statement of Confidentiality This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization. If you have received this communication in error, please notify the sender immediately. If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Bielschowskys Stain
Glawon, Thank you for your help. I appreciate it. Betsy -Original Message- From: Glawon Flood [mailto:smoo...@gmail.com] Sent: Tuesday, March 15, 2011 2:33 PM To: Molinari, Betsy Subject: Re: [Histonet] Bielschowskys Stain Betsy, I don't know of any modifications for Eager's but there are modifications for the Fink-Heimer technique as well as the Nauta-Gygax technique that can be used on paraffin-embedded tissue. I've only come across these in passing and you may have to do a little digging to find a protocol. I hope the info proves useful...good luck! Glawon Flood, HTL (ASCP) Won On Mar 15, 2011, at 11:43 AM, Molinari, Betsy bmolin...@heart.thi.tmc.edu wrote: Thank you for your response. I googled Eagers and found a protocol for Eagers for frozen sections. I have paraffin blocks. Do you know if there is and Eagers method for paraffin? Thank you so much. Betsy -Original Message- From: Glawon Flood [mailto:smoo...@gmail.com] Sent: Tuesday, March 15, 2011 10:22 AM To: Molinari, Betsy Subject: Re: [Histonet] Bielschowskys Stain Hi Betsy, Let me start off by saying that I am by no means an expert on what's preferred. However, in my experience, I've come to find the Bielschowsky method very useful for staining nerve fibers, but I would not consider it so for demonstrating axonal degeneration. I would suggest that you maybe consider Eager's method for degenerating axons. Unlike Bielschowsky's, Eager's actually distinguishes those fibers that are normal from those that are degenerating. I hope this helps. Glawon Flood, HTL (ASCP) On Mar 15, 2011, at 6:00 AM, Molinari, Betsy bmolin...@heart.thi.tmc.edu wrote: Hi , An investigator would like show axonal degeneration in peripheral nerves. Is Bielschowsky's the preferred stain? Thanks! Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Storing slides in buffer
I just did this last week. I stored them in wash buffer overnight in the frig. and they were fine =) Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stacy Deppeler Sent: Wednesday, March 16, 2011 3:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storing slides in buffer Hi guys, I am wondering if anyone has much experience storing slides in buffer (PBS-T) after retrieval for up to about 48hrs at 4C? Is it likely that they will still stain? Or is it going to be entirely dependent on the epitope? I'm optimizing research antibodies right now and I'd like to decrease my turn around time by knocking off the HIER at an earlier time point. Thanks. -- Stacy Deppeler. Research Assistant Department of Ophthalmology King Abdulaziz University Hospital Riyadh, KSA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CONTROLS
Hello everyone, I have an issue that I would like some help with. We are currently running SMM-HC and E-Cad on Breast cores and specimens from a derm lab. With the regulations being so strict regarding the processing times of breast specimens, how are you all dealing with that when it comes to your control tissue? We have to acquire breast tissue from outside sources to use for control, but it is very hard to get it within that 24-72 hour processing time since many hospitals will not release any tissue until the case has been signed out. Does anyone have any wonderful ideas on how to handle that? I would really appreciate it! Thanks histonetters. Sheila Knoxville Dermatopathology Lab Knoxville, TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Procedure for making Gram Control
Hello out there in Histoland, I'm looking for a procedure for making your own Gram controls. Any assistance would be appreciated. Thanks, Randi /pre--- Horizon Health Network Disclaimer ---brbrThis e-mail communication (including any or all attachments) is intendedbronly for the use of the person or entity to which it is addressed and maybrcontain confidential and/or privileged material. If you are not the intendedbrrecipient of this e-mail, any use, review, retransmission, distribution,brdissemination, copying, printing, or other use of, or taking of any action inbrreliance upon this e-mail, is strictly prohibited. If you have received thisbre-mail in error, please contact the sender and delete the original and anybrcopy of this e-mail and any printout thereof, immediately. Yourbrco-operation is appreciated.brbrLe présent courriel (y compris toute pièce jointe) s'adresse uniquement àbrson destinataire, qu'il soit une personne ou un organisme, et pourraitbrcomporter des renseignements privilégiés ou confidentiels. Si vous n'êtesbrpas le destinataire du courriel, il est interdit d'utiliser, de revoir, debrretransmettre, de distribuer, de disséminer, de copier ou d'imprimer cebrcourriel, d'agir en vous y fiant ou de vous en servir de toute autre façon.brSi vous avez reçu le présent courriel par erreur, prière de communiquerbravec l'expéditeur et d'éliminer l'original du courriel, ainsi que toute copiebrélectronique ou imprimée de celui-ci, immédiatement. Nous sommesbrreconnaissants de votre collaboration.pre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Procedure for making Gram Control
Making you own...not sure how to do that, but Slim Jim's work in a pinch. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hayes, Randi (HorizonNB) [randi.ha...@horizonnb.ca] Sent: Wednesday, March 16, 2011 10:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Procedure for making Gram Control Hello out there in Histoland, I'm looking for a procedure for making your own Gram controls. Any assistance would be appreciated. Thanks, Randi /pre--- Horizon Health Network Disclaimer ---brbrThis e-mail communication (including any or all attachments) is intendedbronly for the use of the person or entity to which it is addressed and maybrcontain confidential and/or privileged material. If you are not the intendedbrrecipient of this e-mail, any use, review, retransmission, distribution,brdissemination, copying, printing, or other use of, or taking of any action inbrreliance upon this e-mail, is strictly prohibited. If you have received thisbre-mail in error, please contact the sender and delete the original and anybrcopy of this e-mail and any printout thereof, immediately. Yourbrco-operation is appreciated.brbrLe présent courriel (y compris toute pièce jointe) s'adresse uniquement àbrson destinataire, qu'il soit une personne ou un organisme, et pourraitbrcomporter des renseignements privilégiés ou confidentiels. Si vous n'êtesbrpas le destinataire du courriel, il est interdit d'utiliser, de revoir, debrretransmettre, de distribuer, de disséminer, de copier ou d'imprimer cebrcourriel, d'agir en vous y fiant ou de vous en servir de toute autre façon.brSi vous avez reçu le présent courriel par erreur, prière de communiquerbravec l'expéditeur et d'éliminer l'original du courriel, ainsi que toute copiebrélectronique ou imprimée de celui-ci, immédiatement. Nous sommesbrreconnaissants de votre collaboration.pre CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PRN Position in Longmont Colorado
Hey all, There is a PRN position at Longmont United Hospital open. Working Mon-friday no weekends no evenings. I have attached the link to apply. http://longmontunitedhospital.force.com/Careers/ts2__JobDetails?jobId=a0IA002ct4yMAAtSource= thanks Matt Lunetta HT(ASCP) Longmont United Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Storing slides in buffer
It makes sense that some epitopes might be more sensitive than others. For 48 hours, I would probably store in just PBS, and leave out the detergent (I am assuming the 'T' in your PBST is Tween). Even though Tween is a weak detergent, I would worry that long-term detergent treatment might dissolve the cell membranes or solubilize some proteins, rather than just permeabilizing the membrane. I've successfully done staining for phospho-serine 10-histone 3, cleaved caspase 3, and cardiac troponin T after an extra day stored in PBS at 4 degree. Andrea Marion Graduate Student University of Illinois - Chicago amario3 [at] uic [dot] edu Hi guys, I am wondering if anyone has much experience storing slides in buffer (PBS-T) after retrieval for up to about 48hrs at 4C? Is it likely that they will still stain? Or is it going to be entirely dependent on the epitope? I'm optimizing research antibodies right now and I'd like to decrease my turn around time by knocking off the HIER at an earlier time point. Thanks. -- Stacy Deppeler. Research Assistant Department of Ophthalmology King Abdulaziz University Hospital Riyadh, KSA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Making gram controls
Randi, I don't have a written procedure to forward to you, but this is what we do when we make our gram controls. We use fresh tissue from an autopsy before it goes in formalin (such as lung, we've used placenta before as well). Then put a couple pieces of the fresh lung into containers labeled gram +, and gram - (if you want two pieces of tissue on your control; if you're Pathologists prefer a single tissue on the control with both bugs in them, the you'd only need one container).We get the next steps done with assistance from our Microbiology department. Pour broth (not sure what they use, can get that for you as well if you need) into each of the two containers and then inoculate the gram + container with the appropriate bacteria. Do the same with the gram -'s. Incubate the containers at 37degree's for 24-48hrs. We then pour formalin into the containers and let them fix. Dump the formalin and then put the tissue in labeled cassette's, process them, embed, and cut them to make sure they stain properly and both sets of bacteria are present. We then seal the blocks back up with more paraffin. They seem to last longer that way for us. I googled the topic, and these were steps I found online - although they were for micro, I altered some steps to fit into working for Histo. Katrina Newell, HT(ASCP) Crittenton Hospital Medical Center Rochester, MI 48307 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] S100 A1
I'm looking for a mouse anti human S100 A1 antibody for IHC. My vendor doesn't carry it anymore. Anyone have one that they are using on FFPE tissue? Could you send me ordering info or a copy of your data sheet? Thanks, Angie Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Light hematoxylin staining in H%26EIn-Reply-To=
We seem to be constantly battling this as well. We have found that the quality of the ethanol used in processing and staining can be an issue. We now purchase ours from a company at www.UltraPure.com. The quality of the DI or distilled water being used can also be an issue. We are currently in the process of getting off of our lab building DI water, and purchasing a Elix Advantage Purification system from Millipore. We also found that the type of Eosin can make a big difference. We have recently changed to Eosin from Anatech, and this gives us a very good end product with our Hematoxylin that we still make from scratch--yes we still make our own:) However, the best results we get are when we do not use the DI water that comes from our buildings water system. That is why we are working hard to get our dept our own purification system. It is a very long story how we have come to this decision. But checking into your water supply and the quality of it would be very worthwhile. Very few people realize or understand the importance of water quality for the histology lab. Good luck amysue ruppert __ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP checklist, question ANP.23041.
Regarding CAP checklist, question ANP.23041. The operation of the imaging system is performed by high-complexity testing personnel. We have a question regarding the qualifications of the operators. The operators of the Aperio system are simply scanning entire slides to make a record for us prior to returning slides to the client. Our operators make no evaluation of the image other than whether the scan is adequate for recording purposes, and this is verified by our medical staff prior to release of the materials. In your opinion, does this constitute high-complexity testing and require CLIA compliant qualifications for the operators? Mark Turner, Ph.D, HT(ASCP) QIHC IHC / Histology Manager CSI Laboratories 770-817-0817 x 394 678-205-4669 FAX mtur...@csilaboratories.com mailto:nmon...@csilaboratories.com csilaboratories.com https://csi-srv-007/exchweb/bin/redir.asp?URL=http://www.csi-labs.com/ 11525 Park Woods Circle Alpharetta, GA 30005 Important Warning: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this e-mail is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately and delete the related e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Research and Clinical Labs
For 10 years I ran Histology lab for a large University. We used the same equipment and lab for both animal and human tissue. I had to certify the lab with CLEA for human tissue at one point (and this took serious work!). If you are already certified for human, using the more strict regulations which apply to human tissue, won't hurt the rest, nor increase in any way your time or effort involvement. You do it anyway. I wouldn't leave anything marked for research unless it has proper label with exp date etc. We received everything already fixed and most of the time in cassettes, so there was not much grossing. The processor ran the same night and everything was finished the following day. No one, during any of the inspections had trouble with this. As far as I remember, there was no question about this in any of the check lists sent to me. It was 11 years ago when I parted with my Histo lab and I am not current on the latest regulations, but as far as the technical part... there shouldn't be a problem. Michelle Aloni MS, HTL ASCP USC Keck School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CAP checklist, question ANP.23041.
Mark this is a new question and let me answer this for you from a inspection point of view. Currently there are 15 to 18 new questions that deal with predicative markers and the digital images, most of these are QA/QC related. But the issue with images is that your people are inspecting them. When you are looking at an image it is more than just click and go. There are technical issues of Tiling, focus with z stacking, AOI ( if the full image was captured), magnification, etc. This is just technical to images, but then there are the aspects that are to the histology world as in staining components, microtomy, fixation, etc. To say that they don't make a decision is a huge understatement. As far as I know CAP put this in because people were just scanning images and sending them off. There are also issues were the scanner scans for possible algorithm studies. You have to make sure this is inline. So as for the High complexity testing to what CLIA defines, that is a back and forth issue. Currently within our facility you have to be licensed HT/HTL to do scanning. This comes in line with the CAP looking at creating a QM program and having the technician/Technologist maintain competencies and assessments for this technology. There is also a competency and assessment due for the pathologist as well for this technology. I can go on and on, but you can contact me off line for this. Jesus Ellin Yuma Regional Medical Center 928-336-1743 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Wednesday, March 16, 2011 4:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP checklist, question ANP.23041. Regarding CAP checklist, question ANP.23041. The operation of the imaging system is performed by high-complexity testing personnel. We have a question regarding the qualifications of the operators. The operators of the Aperio system are simply scanning entire slides to make a record for us prior to returning slides to the client. Our operators make no evaluation of the image other than whether the scan is adequate for recording purposes, and this is verified by our medical staff prior to release of the materials. In your opinion, does this constitute high-complexity testing and require CLIA compliant qualifications for the operators? Mark Turner, Ph.D, HT(ASCP) QIHC IHC / Histology Manager CSI Laboratories 770-817-0817 x 394 678-205-4669 FAX mtur...@csilaboratories.com mailto:nmon...@csilaboratories.com csilaboratories.com https://csi-srv-007/exchweb/bin/redir.asp?URL=http://www.csi-labs.com/ 11525 Park Woods Circle Alpharetta, GA 30005 Important Warning: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this e-mail is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately and delete the related e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP checklist, question ANP.23041.
Well I wouldn't try and use a Ph.D. in religous studies to qualify for high complexity testing... On Wed, Mar 16, 2011 at 4:36 PM, Mark Turner mtur...@csilaboratories.comwrote: Regarding CAP checklist, question ANP.23041. The operation of the imaging system is performed by high-complexity testing personnel. We have a question regarding the qualifications of the operators. The operators of the Aperio system are simply scanning entire slides to make a record for us prior to returning slides to the client. Our operators make no evaluation of the image other than whether the scan is adequate for recording purposes, and this is verified by our medical staff prior to release of the materials. In your opinion, does this constitute high-complexity testing and require CLIA compliant qualifications for the operators? Mark Turner, Ph.D, HT(ASCP) QIHC IHC / Histology Manager CSI Laboratories 770-817-0817 x 394 678-205-4669 FAX mtur...@csilaboratories.com mailto:nmon...@csilaboratories.com csilaboratories.com https://csi-srv-007/exchweb/bin/redir.asp?URL=http://www.csi-labs.com/ 11525 Park Woods Circle Alpharetta, GA 30005 Important Warning: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this e-mail is not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately and delete the related e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
