Re: [Histonet] Controls for IPs
This response is late due to the overwhelming time commitments of NCAA Basketball Tournaments. The only multi-tissue control we use is for HER2 protein overexpression. In my opinion, multi-tissue controls are completely unnecessary for every-day use for the majority of IHC tests that we do. They are expensive and time-consuming to prepare and, as you pointed out, consume valuable tissue. Multi-tissue controls should be used during primary antibody optimization and validation studies; they are not needed for routine testing. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Bauer, Karen L. bauer.ka...@mayo.edu 3/8/2011 10:16 AM Hello, It seems we go back and forth between multi-tissue control blocks and single tissue control blocks when it comes to immunostaining. In the past we used single blocks and still continue to use some for the IPs that are rarely used. Then, switched to creating multi-tissue blocks for ease of use. It's so nice to cut control slides ahead of time and just pull them out and add our patient tissue. But... we find that the multi-tissue blocks are using up hard to find tissue. For example, we have a melanoma skin in one of the MTBs for the HMB45 and Melan A stains, but that MTB is used more for the CK7, CK20 and TTF-1 stains. Basically, that melanoma skin is just being wasted. I'm considering going back to the single block controls and was wondering what other labs are doing. What is the concensus in HistoLand in regards to IP control blocks/tissues? Thanks much!! Karen Karen L. Bauer HTL/HT (ASCP) Histology Supervisor Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System bauer.ka...@mayo.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD4, CD8 on Monkey tissue
HI everyone, I wanted to see if anyone out there has had any luck using any CD4 and CD8 antibodies on FFPE monkey tissue. I have used a couple of Ab's that work great on human tissue but no luck with monkey tissue. Any information will be greatly appreciated. Thanks, Pedro Louro, MBA, QIHC IHC Technologist HLS CRO Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Control Tissue
Does anyone have or know where I can get control tissue for the MYCN (2p 24.1) FISH probe assay, an amplified or positive control? Thanks Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metz...@aruplab.com - -- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT Position in Oklahoma City
St. Anthony Hospital currently has an excellent opportunity for an experienced Histology Technician. This position requires Certification as an HT or HLT – or – experience acceptable to the Laboratory Director. Two years of previous histology experience required with IHC and/or grossing experience a plus. Outstanding benefits package, including generous paid time off. For consideration, please apply online at www.saintsok.comhttp://www.saintsok.com/https://mobile.ssmhc.com/owa/UrlBlockedError.aspxhttps://mobile.ssmhc.com/owa/UrlBlockedError.aspxhttps://mobile.ssmhc.com/owa/UrlBlockedError.aspx, Ad # 10762, or contact Anna King for additional information at (405) 272-6105. Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Movat's Pentachrome Question
Happy Friday everyone!! I am trying to find a good Modified Movat's pentachrome stain protocol for plastic embedded iliac arteries and also bone tissue (animal model). Everything I have been able to find so far, is for paraffin embedded tissue and a mile long. There may not be a fix for the length of the protocol but if anyone would happen to have a technique that works well with plastic, I would greatly appreciate it. Thank you much. Have a great weekend!! Jim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] coverslippers
Hi Everyone! Our small core is looking into getting a coverslipper and I was wondering the following things: 1) Which company did you get yours from? 2) Is there a difference between glass vs. plastic? (thinking of allowing people who do IHC/IMF to use it too) 3) What are the pros and cons of your machine? The world of coverslippers is huge and I'm just getting into the automation---FINALLY!! Would love to hear from the experienced! Thanks so much for all of your help in advance! I truly appreciate it! Jen Jennifer K. Sipes, ALAT Sr. Laboratory Technician Johns Hopkins University Ross 929 720 Rutland Avenue Baltimore, MD 21205 phone: 410-614-0131 410-955-9688 fax: 410-955-9677 cell: 443-631-6361 e-mail: jsip...@jhmi.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: QC documentation
Let me add the dyspeptic observation that, in the many pathology services I've worked in in the last 30 years - the more QC paperwork I have to do, the worse the slides. I've never seen it done, but I think the only effective QC program in histology would be daily review of selected slides by a pathologist and a senior histotechnologist, with documentation of what problems were observed and what got done about them. I'm sure neither Six Sigma nor LEAN (whatever they may be) would approve of this Edwards Deming approach. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica Cryostat Service Recommendations
Hello Histonetters!!! I am writing today to ask about the necessity of having cryostats serviced regularly. We have a Leica CM 3050S that is about 2 years old now. I have been looking far and wide for recommendations about cryostat maintenance, but haven't found much information. Do you think it is necessary to purchase a service contract that includes having someone come out regularly to check on the equipment? We love our Leica rep, but I haven't wanted to contact him about this until I have a better idea of what other labs are doing. I should mention that ours is a very small lab, with sectioning time averaging only about 5 hours per week. Thanks for any advice! Amanda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] coverslippers
We have the tape coverslipper from Sakura and have had it for years we love it. Yo need to make sure when you change the tape that it is allowed to sit out of the package for a little while. We put one on the machine and open a new one and let it sit out- but I think overnight is fine. It's very low maintenance. Lisa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Sipes Sent: Friday, April 08, 2011 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] coverslippers Hi Everyone! Our small core is looking into getting a coverslipper and I was wondering the following things: 1) Which company did you get yours from? 2) Is there a difference between glass vs. plastic? (thinking of allowing people who do IHC/IMF to use it too) 3) What are the pros and cons of your machine? The world of coverslippers is huge and I'm just getting into the automation---FINALLY!! Would love to hear from the experienced! Thanks so much for all of your help in advance! I truly appreciate it! Jen Jennifer K. Sipes, ALAT Sr. Laboratory Technician Johns Hopkins University Ross 929 720 Rutland Avenue Baltimore, MD 21205 phone: 410-614-0131 410-955-9688 fax: 410-955-9677 cell: 443-631-6361 e-mail: jsip...@jhmi.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica Cryostat Service Recommendations
We have the same cryostat, three years old and this is the first time anything has broken on it. The total cost for everything will be about $600 which is wy cheaper than a $4000 service contract (for one year!). Also we use our cryostat almost every day, all day. If you're using it that little, and it's working fine, I would say hold off on the service contract. Just keep it clean and if it stops working, turn it off and let it defrost for a few days. Usually that fixes everything because it's just iced over somewhere. Leica service contracts are a complete waste of money--only get them if you need to spend money (which was the case for us a few years ago, unfortunately, it's not now!). Also, don't get Leica to fix your cryostat! They charge twice as much as anyone else. One thing to remember to save your object cooling compressor motor, when you restart your cryostat after defrosting, cool the chamber first, then specimen head. That way, the specimen head (object temperature) compressor will not have to work as hard to keep itself cool while the chamber is cooling. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Fri, Apr 8, 2011 at 3:11 PM, Amanda Madden amkmad...@gmail.com wrote: Hello Histonetters!!! I am writing today to ask about the necessity of having cryostats serviced regularly. We have a Leica CM 3050S that is about 2 years old now. I have been looking far and wide for recommendations about cryostat maintenance, but haven't found much information. Do you think it is necessary to purchase a service contract that includes having someone come out regularly to check on the equipment? We love our Leica rep, but I haven't wanted to contact him about this until I have a better idea of what other labs are doing. I should mention that ours is a very small lab, with sectioning time averaging only about 5 hours per week. Thanks for any advice! Amanda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica Cryostat Service Recommendations
I'll second that, we have 4 of these (all 6 yrs. old) and are very happy with them. We do not have service contracts and, when needed, have a local company come in for repairs and PMs. Good tip Emily about the object cooling compressor, thanks! Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, April 08, 2011 3:24 PM To: Amanda Madden; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Leica Cryostat Service Recommendations We have the same cryostat, three years old and this is the first time anything has broken on it. The total cost for everything will be about $600 which is wy cheaper than a $4000 service contract (for one year!). Also we use our cryostat almost every day, all day. If you're using it that little, and it's working fine, I would say hold off on the service contract. Just keep it clean and if it stops working, turn it off and let it defrost for a few days. Usually that fixes everything because it's just iced over somewhere. Leica service contracts are a complete waste of money--only get them if you need to spend money (which was the case for us a few years ago, unfortunately, it's not now!). Also, don't get Leica to fix your cryostat! They charge twice as much as anyone else. One thing to remember to save your object cooling compressor motor, when you restart your cryostat after defrosting, cool the chamber first, then specimen head. That way, the specimen head (object temperature) compressor will not have to work as hard to keep itself cool while the chamber is cooling. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Fri, Apr 8, 2011 at 3:11 PM, Amanda Madden amkmad...@gmail.com wrote: Hello Histonetters!!! I am writing today to ask about the necessity of having cryostats serviced regularly. We have a Leica CM 3050S that is about 2 years old now. I have been looking far and wide for recommendations about cryostat maintenance, but haven't found much information. Do you think it is necessary to purchase a service contract that includes having someone come out regularly to check on the equipment? We love our Leica rep, but I haven't wanted to contact him about this until I have a better idea of what other labs are doing. I should mention that ours is a very small lab, with sectioning time averaging only about 5 hours per week. Thanks for any advice! Amanda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Movat's Pentachrome Question
Ummm...yes, the protocol for a Movat's Pentachrome is a mile long. It's got five different stains There is no one step Movat's Pentachrome. As a matter of fact, to me, it's always been shorthand for a pain in the a**. Tech one: Any special stains today? I need to leave early. Tech two: Just a Movat's Pentachrome. Tech one: %*#@$ !!! Sincerely, Jay A. Lundgren, M.S., HTL(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Breast fixation over the weekend
What percentage of alcohol are you using to hold breast over the weekend, so as not to interfere with Her2 breast markers? Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica Cryostat Service Recommendations
Thank you everyone for, as usual, all of the fantastic advice! Glad to know that we can save some money and still know that we're taking good care of our favorite tool!! Amanda On Fri, Apr 8, 2011 at 3:47 PM, Connolly, Brett M brett_conno...@merck.comwrote: I'll second that, we have 4 of these (all 6 yrs. old) and are very happy with them. We do not have service contracts and, when needed, have a local company come in for repairs and PMs. Good tip Emily about the object cooling compressor, thanks! Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Friday, April 08, 2011 3:24 PM To: Amanda Madden; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Leica Cryostat Service Recommendations We have the same cryostat, three years old and this is the first time anything has broken on it. The total cost for everything will be about $600 which is wy cheaper than a $4000 service contract (for one year!). Also we use our cryostat almost every day, all day. If you're using it that little, and it's working fine, I would say hold off on the service contract. Just keep it clean and if it stops working, turn it off and let it defrost for a few days. Usually that fixes everything because it's just iced over somewhere. Leica service contracts are a complete waste of money--only get them if you need to spend money (which was the case for us a few years ago, unfortunately, it's not now!). Also, don't get Leica to fix your cryostat! They charge twice as much as anyone else. One thing to remember to save your object cooling compressor motor, when you restart your cryostat after defrosting, cool the chamber first, then specimen head. That way, the specimen head (object temperature) compressor will not have to work as hard to keep itself cool while the chamber is cooling. Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Fri, Apr 8, 2011 at 3:11 PM, Amanda Madden amkmad...@gmail.com wrote: Hello Histonetters!!! I am writing today to ask about the necessity of having cryostats serviced regularly. We have a Leica CM 3050S that is about 2 years old now. I have been looking far and wide for recommendations about cryostat maintenance, but haven't found much information. Do you think it is necessary to purchase a service contract that includes having someone come out regularly to check on the equipment? We love our Leica rep, but I haven't wanted to contact him about this until I have a better idea of what other labs are doing. I should mention that ours is a very small lab, with sectioning time averaging only about 5 hours per week. Thanks for any advice! Amanda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
