Re: [Histonet] thermometer
A pyrometer should do the job, I guess. My last CLIA inspection, I was told to check the temperature in the wax chamber of my embedding station to compare against the temperature it says it is...does anyone know of a good thermometer to purchase? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Question about delayed start in Leica Tissue Processor
The easiest way to remember is to count the midnights between starting and ending - therefore today will be 0, tomorrow 1etc On Mon, Apr 18, 2011 at 6:24 PM, Jenny Vega histotech...@gmail.com wrote: Yes, that is what makes sense to me. Tomorrow morning after removing the specimens from the tissue basket I will check how my supervisor set the clock last Friday. If she chose 3-18:20, then by logic I will have to chose 4-18:20, since I will be away for the holidays for 4 days straight and I need the tissues to be processed by the next monday (april 25). I was told that the tissue processor starts at 6pm and it is done the next morning. I did not understood military time before and now I know that 18:20 means 6:20pm. Thanks On Mon, Apr 18, 2011 at 8:39 AM, Thomas, Nancy n...@stowers.org wrote: We have the same processor. If you are returning to work next Monday morning, then you would choose the 4 day delay. It would begin processing at 18:20 Sunday night and be ready on Monday morning. Nancy Thomas Stowers Institute -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jenny Vega Sent: Sunday, April 17, 2011 8:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about delayed start in Leica Tissue Processor Hello. I have little experience using the tissue processor from my lab which is an old version of the Leica TP1020. I was taugh that since on weekends the processor is not going to be used then you have to set up the days of delay for example 2-18:20 (2 represents saturday and sunday). On Monday (tomorrow) I have the day off, so the processor won't be used. When I come back on Tuesday I have to change the the time as 0-18:20 so that the processor starts working that day and on wednesday. On thursday, friday, saturday and Sunday will also have the days off. I am confused because my supevisor told me to set the time as 3-18:20 next Wednesday, and since I will not be working in the lab for 4 days straight that does not make sense, it is suppose to be 4-18:20 instead. I think she made a mistake telling me that since maybe she confused the program of this week when the processor was programmed as 3-18:20 because used for 3 days straight (saturday to monday). My supervisor won't be in the lab next week so I cannot ask her. This question seems silly but I want to be sure. I know that if tissue is left for a long time in paraffin the tissue can get over-harden and I don't want to program de delayed start as 3-18:20 thinking it is the correct way when is not. Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue adhesion to slides
Hello out there! I was wondering if any of you have experience in attaching human retinae (~250-300 microns thick) to slides for manual immunohistochemistry. Starfrost does not seem adequate. Any advice would be greatly appreciated! Regards, Abbey Abbey Mortimer PhD Candidate School of Psychological Science La Trobe University Bundoora, Victoria, 3083 AUSTRALIA Phone: (03) 9479 2470 Email: a.morti...@latrobe.edu.au ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cytotech job opening in Brooklyn NY
There is a full time position open for a Cytotechnologist in Brooklyn, NY. A candidate with Histology background is preferred. Please forward letter of interest and resume to Ellen Barbarino ebarbar...@siradonc.com Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Neuronal staining mouse limb sections
Hello, I'm investigating a potential neuronal defect of our transgenic mouse embryos. Until now the staining I tried (luxol Fast Blue) didn't work out. I was wondering now if there is an alternative for this. Is there a staining (or an Ab), that I can use to detect any kind of neurons in the limb of a mouse embryo? I'm looking for a general detection of neurons. Preferentially I would like it to work on frozen sections, but for now anything would work for me! Thank you! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Neuronal staining mouse limb sections
Hi Hendrik, Try rabbit neuro-specific beta-III tubulin antibody (Abcam ab18207). Works fine on frozen and paraffin sections. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: agleiber...@cbiolabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hendrik Mommaerts Sent: Tuesday, April 19, 2011 9:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Neuronal staining mouse limb sections Hello, I'm investigating a potential neuronal defect of our transgenic mouse embryos. Until now the staining I tried (luxol Fast Blue) didn't work out. I was wondering now if there is an alternative for this. Is there a staining (or an Ab), that I can use to detect any kind of neurons in the limb of a mouse embryo? I'm looking for a general detection of neurons. Preferentially I would like it to work on frozen sections, but for now anything would work for me! Thank you! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC on frozen sections
Following is question on behalf of Jana Moose Ritter, DVM janamo...@yahoo.com We are going to perform IHC on frozen sections, and the tissues (pig aorta) will be harvested on a Friday. Histo lab is wondering: 1) can tissue fix in paraformaldehyde over the weekend until next steps (sucrose and frozen embedding to be completed on Monday) 2) can fixative be switched to sucrose at the end of Friday (tissue would remain in sucrose until frozen embedding on Monday) 3) must complete the solution switch and entire frozen embedding process on Saturday Thanks for your help. Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question
Good afternoon: I am Carmen Garcia a PhD student from the Faculty of medicine from the University of Valencia.We work with cryocut of mouse ovary, now I want to see the filopodia of the endothelial cells, do you have any suggestion about what I should do? Thank you so much, Carmen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Miller's Elastin Stain
** Proprietary ** ** Reply Requested When Convenient ** Hi there, Does anyone use commercial Miller's Elastic Stain other than VWR Ref 35115S one ? If so I would be grateful if you could let me know which one you use Thanks in advance Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 ben...@gosh.nhs.uk * This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: [IHCRG] Ventana EBER DNP
SAME HERE!!! I would love some help with this. We are going to start doing the EBER with Ventana and have no idea where to start. Thanks in advance for ANY suggestions or help you could give. Sheila, HT (ASCP) KDL Pathology Knoxville, TN From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of Tallardy, Betty S Sent: Tuesday, April 19, 2011 10:55 AM To: ih...@googlegroups.com Subject: [IHCRG] Ventana EBER DNP Hi Group, Is anyone stuck in the EBER transition with Ventanas' probe changeover? The new product is supplied in a small vial and of course there are no suggestions about how to use the product. If anyone has some ideas on how to proceed with this, I would really appreciate some suggestions. I hate to waste such a pricey product trying to get something workable. Thanks in advance. Betty Tallardy, HT(ASCP)QIHC Dept. of Pathology Presbyterian Hospital 200 Hawthorne Lane Charlotte, NC 28204 704-384-5490 bstalla...@novanthealth.org _ This message and any included attachments are from NOVANT HEALTH INC. and are intended only for the addressee(s). The information contained herein may include trade secrets or privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. If you believe that any information contained in this message is disparaging or harassing or if you find it objectionable please contact Novant Health, Inc. at 1-800-350-0094 or forward the e-mail to repo...@novanthealth.org. Thank you. -- You received this message because you are subscribed to the Google Groups ihcrg group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ih...@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscr...@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: hi...@nsh.org or call 443.535.4060. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: [IHCRG] Ventana EBER DNP
If anyone has the catalog numbers for the probes, that would help. I couldn't get this info from Ventana when I asked. They said we have to ask another lab, even for catalog numbers! Mark On Tue, Apr 19, 2011 at 7:59 AM, Sheila Fonner sfon...@labpath.com wrote: SAME HERE!!! I would love some help with this. We are going to start doing the EBER with Ventana and have no idea where to start. Thanks in advance for ANY suggestions or help you could give. Sheila, HT (ASCP) KDL Pathology Knoxville, TN From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of Tallardy, Betty S Sent: Tuesday, April 19, 2011 10:55 AM To: ih...@googlegroups.com Subject: [IHCRG] Ventana EBER DNP Hi Group, Is anyone stuck in the EBER transition with Ventanas' probe changeover? The new product is supplied in a small vial and of course there are no suggestions about how to use the product. If anyone has some ideas on how to proceed with this, I would really appreciate some suggestions. I hate to waste such a pricey product trying to get something workable. Thanks in advance. Betty Tallardy, HT(ASCP)QIHC Dept. of Pathology Presbyterian Hospital 200 Hawthorne Lane Charlotte, NC 28204 704-384-5490 bstalla...@novanthealth.org _ This message and any included attachments are from NOVANT HEALTH INC. and are intended only for the addressee(s). The information contained herein may include trade secrets or privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. If you believe that any information contained in this message is disparaging or harassing or if you find it objectionable please contact Novant Health, Inc. at 1-800-350-0094 or forward the e-mail to repo...@novanthealth.org. Thank you. -- You received this message because you are subscribed to the Google Groups ihcrg group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ih...@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscr...@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.orgor call 443.535.4060. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: [IHCRG] Ventana EBER DNP
Your sales rep should supply these to you. I'm having problems with filling my ISH Probe Kit. I logged in my EBER Probe. I registered the Probe kit and went to fill it and it only lets me pick from my Fillable Reagents list, not the Fillable probes list. Tech support can't figure out why this is happening and said they haven't encountered this problem with any other clients. Anyone else having this issue? Mark Tarango marktara...@gmail.com 4/19/2011 11:08 AM If anyone has the catalog numbers for the probes, that would help. I couldn't get this info from Ventana when I asked. They said we have to ask another lab, even for catalog numbers! Mark On Tue, Apr 19, 2011 at 7:59 AM, Sheila Fonner sfon...@labpath.com wrote: SAME HERE!!! I would love some help with this. We are going to start doing the EBER with Ventana and have no idea where to start. Thanks in advance for ANY suggestions or help you could give. Sheila, HT (ASCP) KDL Pathology Knoxville, TN From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of Tallardy, Betty S Sent: Tuesday, April 19, 2011 10:55 AM To: ih...@googlegroups.com Subject: [IHCRG] Ventana EBER DNP Hi Group, Is anyone stuck in the EBER transition with Ventanas' probe changeover? The new product is supplied in a small vial and of course there are no suggestions about how to use the product. If anyone has some ideas on how to proceed with this, I would really appreciate some suggestions. I hate to waste such a pricey product trying to get something workable. Thanks in advance. Betty Tallardy, HT(ASCP)QIHC Dept. of Pathology Presbyterian Hospital 200 Hawthorne Lane Charlotte, NC 28204 704-384-5490 bstalla...@novanthealth.org _ This message and any included attachments are from NOVANT HEALTH INC. and are intended only for the addressee(s). The information contained herein may include trade secrets or privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. If you believe that any information contained in this message is disparaging or harassing or if you find it objectionable please contact Novant Health, Inc. at 1-800-350-0094 or forward the e-mail to repo...@novanthealth.org. Thank you. -- You received this message because you are subscribed to the Google Groups ihcrg group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ih...@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscr...@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.orgor call 443.535.4060. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] [Histonet Biopsy Program for Sakura VIP 5/6 Processor
Try to eliminate one of the 100% Alcohols. If it cannot eliminate it, then just pump it in and out. Microwave processing actually uses Ethanol, Isopropyl, and paraffin only. Toysha N. Mayer, MBA, HT (ASCP) Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org Message: 8 Date: Tue, 12 Apr 2011 14:57:18 -0400 From: Evans, Andria B aeva...@lghealth.org Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 4182fdf23d7c9948bc41c4c082c3a54f021557b3a...@mail-ag-cluster.lha.org Content-Type: text/plain; charset=iso-8859-1 Our lab is currently looking for a way to shorten our Biopsy processing program without compromising the patient specimen. We do have an issue with our GI's being very dry, which causes us to have to soak between each level taken and also causes a lot of chatter. Also we have a goal to do a run during the day to improve turn around time. Here is what our current protocol is Formalin1 min 37degrees Formalin15 mins37degrees 70 14 mins40 degrees 95 14 mins40 degrees 95 9 mins 40 degrees 100 9 mins 40 degrees 100 7 mins 40 degrees 100 4 mins 40 degrees Xylene 23 minsno heat Xylene 15 minsno heat Paraffin 20 mins 60 degrees Paraffin 18 mins 60 degrees Paraffin 10 mins 60 degrees Paraffin 0 mins 60 degrees All the steps are set on a fast mix setting. All of our biopsy specimens are put into sponges. Any feedback would be greatly appreciated. Andria B Evans HTL(ASCP)CM Lancaster General Hospital 555 North Duke Street Lancaster, PA 17604 (717)544-5511 ext: 77329 aeva...@lgheath.orgmailto:aeva...@lgheath.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] thermometer
You can bay a certified digital thermometer for less than $35 and will be more than adequate for what you need. René J. --- On Mon, 4/18/11, Amber McKenzie amber.mcken...@gastrodocs.net wrote: From: Amber McKenzie amber.mcken...@gastrodocs.net Subject: [Histonet] thermometer To: histonet@lists.utsouthwestern.edu Date: Monday, April 18, 2011, 5:06 PM My last CLIA inspection, I was told to check the temperature in the wax chamber of my embedding station to compare against the temperature it says it is...does anyone know of a good thermometer to purchase? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tissue adhesion to slides
Such thick sections are usually handled better if stained floating (not adhered to sledes). René J. --- On Tue, 4/19/11, Abbey Mortimer a.morti...@latrobe.edu.au wrote: From: Abbey Mortimer a.morti...@latrobe.edu.au Subject: [Histonet] Tissue adhesion to slides To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Date: Tuesday, April 19, 2011, 5:00 AM Hello out there! I was wondering if any of you have experience in attaching human retinae (~250-300 microns thick) to slides for manual immunohistochemistry. Starfrost does not seem adequate. Any advice would be greatly appreciated! Regards, Abbey Abbey Mortimer PhD Candidate School of Psychological Science La Trobe University Bundoora, Victoria, 3083 AUSTRALIA Phone: (03) 9479 2470 Email: a.morti...@latrobe.edu.au ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] in-situ hybridization
This questions is for the labs that do in-situ hybridization for diagnostic purposes. Do you take slides for ISH from other labs and if so, please contact me via my email. My next question is; one of my doc's is interested in us doing ISH herein our lab but I have been putting her off with the fact that we do not need this very often, it is very expensive to start up, etc. But she is persistent. Any suggestions for starting up this procedure, including vendor preferences for supplies? I know next to nothing about ISH other than what I have read on-line. Thank you in advance Candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] slide drying ovens
I'm looking for recommendations for a new slide drying oven. Something relatively small, with convection. Any input would be appreciated. Thanks ! Mandy M Bell , HTL (ASCP) Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Disposing of gross only items
Hello Everyone, Need some info on the disposal of gross only items. These items include hardware, wires, gallstones and foreign objects. We are running out of room for the storage of gross only specimens. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] slide drying ovens
There are many so called convection ovens out there that are gravity convection. Biocare has a great convection oven that has an internal fan, and can be set with several different programs. You can set it for IHC at the end of the day and it automatically stops and is ready for you in the AM when you come in. It has a small footprint. It is not cheap, but well worth it. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Apr 19, 2011, at 10:13 AM, Bell, Mandy wrote: I'm looking for recommendations for a new slide drying oven. Something relatively small, with convection. Any input would be appreciated. Thanks ! Mandy M Bell , HTL (ASCP) Histology Department Community Hospital of the Monterey Peninsula 831.625.4791 P Please consider the environment before printing this e-mail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rabbit eye holder?
Hi all, I'm looking for something to hold my rabbit eyes in place (and other eyes if they're available like rat) while cutting them in half before further processing. My boss said he used them a long time ago but doesn't know what they're called. I can't figure out what they'd be called either but I'd like to buy some if they exist. Any input? Ideas? Thanks! Laura ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 3, 000 Breast Cancer Specimens from Quebec Breast Cancer being retested
PhenoPath Laboratories Retests Nearly 3,000 Breast Cancer Specimens from Quebec Breast Cancer specimens which may have been misdiagnosed. In the re-analysis, results of which were just announced, a total of 87 women were found to have received a false negative result from the original pathology laboratory tests in Quebec. Of this number, 39 were required to alter their treatment and among them five women died. Minister of Health Yves Bolduc said it was impossible to determine whether the women died as a result of being improperly treated. Check out this link. http://www.ihc.co/a824362-seattle-based- phenopath-laboratories-retests-nearly-.cfm Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] unsubscribe
Sponsored by Catholic Health Initiatives and Immanuel, Alegent Health is faithful to the healing ministry of Jesus Christ, providing high quality care for the body, mind and spirit of every person. The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. Alegent Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] in-situ hybridization
Roche Diagnostics has great DIG labels, antibodies and color reaction chemicals. It is a pain to just start up, you need to buy a lot of stuff for it. Could you use someone else's set up and pay for part of the chemicals? Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Tue, Apr 19, 2011 at 12:51 PM, Bales, Candy A candy.a.ba...@uth.tmc.eduwrote: This questions is for the labs that do in-situ hybridization for diagnostic purposes. Do you take slides for ISH from other labs and if so, please contact me via my email. My next question is; one of my doc's is interested in us doing ISH herein our lab but I have been putting her off with the fact that we do not need this very often, it is very expensive to start up, etc. But she is persistent. Any suggestions for starting up this procedure, including vendor preferences for supplies? I know next to nothing about ISH other than what I have read on-line. Thank you in advance Candy Candy Bales, HT Chief Histologist The University of Texas Dental Branch at Houston Diagnostic Sciences-Oral Pathology 6516 M.D. Anderson Blvd. # 3.093 Houston, TX 77030 713.500.4411 office 713.500.4416 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Standardization??? 3, 000 Breast Cancer Specimens from Quebec Breast Cancer being retested
From what I have experienced in my observations of various labs; numerous labs do not follow proper standardization practices which are set by FDA guidelines for Her2-neu. Prognostic markers are particularly susceptible to heat. Baking slides in excess temperatures (over 62 degrees C) for prolonged lengths of time can also drop the antigenicity of the test tissues a full grade. There has been documentation of this through publications. Temperatures MUST be monotored and recorded. The Antigen Retrieval Process is very important and QC measures MUST be followed. Internal temperature and pressure MUST be met and recorded. QC monitor strips will record and show any irregularities in this step. If the pressure and temp has not been met, the test should be repeated. A lot of IHC techs do not monitor this process. Unfortunately, internal laboratory standardization can vary from shift to shift. Just think about non- standardization practices throughout the country because of under- education and strict adherence to established guidelines. Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com On Apr 19, 2011, at 10:36 AM, Akemi Allison wrote: PhenoPath Laboratories Retests Nearly 3,000 Breast Cancer Specimens from Quebec Breast Cancer specimens which may have been misdiagnosed. In the re-analysis, results of which were just announced, a total of 87 women were found to have received a false negative result from the original pathology laboratory tests in Quebec. Of this number, 39 were required to alter their treatment and among them five women died. Minister of Health Yves Bolduc said it was impossible to determine whether the women died as a result of being improperly treated. Check out this link. http://www.ihc.co/ a824362-seattle-based-phenopath-laboratories-retests-nearly-.cfm Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Biopsy Program for Sakura VIP 5/6 Processor
Wow, somehow I missed this email. Sorry for the delay. Andria, take the heat off your alcohols. Keep it on the formalin and the paraffin, but do the rest of the processing at ambient temperature. Make sure you put only small soft tissues on this schedule though, tougher collagenous skin specimens might not process well enough with the quick dehydration times you have. I am curious how you arrived at the timing for the steps. 14 minutes, 9 minutes, 7 minutes, 23 minutes, etc? Good luck and best wishes, Teri Teri Johnson, HT(ASCP)QIHC Head, Histology and Electron Microscopy Stowers Institute for Medical Research Kansas City, MO ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC validation
Does a change in the type of hematoxylin counterstain require any re-validation of IHC stains? Thanks for your help! Mary Ring, HT, QIHC Regions Hospital, St Paul, Mn This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Microarray
Are you using tissue microarrays as controls for all your routine IHC stains (not antibody validation or research) ? If so, how are you making the arrays? thank you, -Kiranjit ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Disposing of gross only items
First talk to your legal department, just in case! René J. --- On Tue, 4/19/11, Stella Mireles estellamire...@gmail.com wrote: From: Stella Mireles estellamire...@gmail.com Subject: [Histonet] Disposing of gross only items To: Histonet@lists.utsouthwestern.edu Date: Tuesday, April 19, 2011, 1:22 PM Hello Everyone, Need some info on the disposal of gross only items. These items include hardware, wires, gallstones and foreign objects. We are running out of room for the storage of gross only specimens. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC validation
Not really because the Hematoxylin should affect only the nuclei UNLESS it is a nuclear antibody, in which case the antigenic reaction could be obscured. For nuclear Abs I used to dilute my hematoxylin as much as possible or did not use it at all. René J. --- On Tue, 4/19/11, Ring, Mary L mary.l.r...@healthpartners.com wrote: From: Ring, Mary L mary.l.r...@healthpartners.com Subject: [Histonet] IHC validation To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Tuesday, April 19, 2011, 3:04 PM Does a change in the type of hematoxylin counterstain require any re-validation of IHC stains? Thanks for your help! Mary Ring, HT, QIHC Regions Hospital, St Paul, Mn This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC validation
In my opinion, only if it affects immunoreactivity. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Ring, Mary L mary.l.r...@healthpartners.com 4/19/2011 3:04 PM Does a change in the type of hematoxylin counterstain require any re-validation of IHC stains? Thanks for your help! Mary Ring, HT, QIHC Regions Hospital, St Paul, Mn This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AUTO: Carolyn J Kent is out of the office. (returning 04/21/2011)
I am out of the office until 04/21/2011. I will respond to your message when I return. Note: This is an automated response to your message Histonet Digest, Vol 89, Issue 21 sent on 4/19/2011 12:02:59 PM. This is the only notification you will receive while this person is away. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] whole retinal staining
Dear Abbey Histonet I agree with René Buesa: I would try to stain whole retinae by a 'floating section' method i.e. hand processing in a dipping tray or mesh baskets (brush transfer can be done too but is laborious). Then mount them at the end. Floating allows reagents to penetrate from both sides of the retina - extra valuable because your incubation times are going to be very long with material this thick. (I assume you are not just interested in superficial layers.) A microwave processor would help with penetration, but I have no direct experience. -David == David A. Wright, Ph.D. University of Chicago Section of Neurosurgery ORIGINAL POST: Histonet Digest, Vol 89, Issue 21 Message: 7 Date: Tue, 19 Apr 2011 19:00:27 +1000 From: Abbey Mortimer a.morti...@latrobe.edu.au Subject: [Histonet] Tissue adhesion to slides Hello out there! I was wondering if any of you have experience in attaching human retinae (~250-300 microns thick) to slides for manual immunohistochemistry. Starfrost does not seem adequate. Any advice would be greatly appreciated! Regards, Abbey Abbey Mortimer PhD Candidate School of Psychological Science La Trobe University Bundoora, Victoria, 3083 AUSTRALIA Phone: (03) 9479 2470 Email: a.morti...@latrobe.edu.au ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IHC on frozen sections
Jana, Since you are fixing in formaldehyde, why not paraffin embed them? Frozen sectioning of formalin fixed tissue and doing IPX on them is difficult as well as problems with storing the tissue. I would cut frozen of unfixed tissue and do IPX on them rather than doing IPX on formalin fixed frozen sections. Any reason why you have chosen the frozen section of formalin fixed tissue? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jane C. Moose Sent: Wednesday, 20 April 2011 12:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on frozen sections Following is question on behalf of Jana Moose Ritter, DVM janamo...@yahoo.com We are going to perform IHC on frozen sections, and the tissues (pig aorta) will be harvested on a Friday. Histo lab is wondering: 1) can tissue fix in paraformaldehyde over the weekend until next steps (sucrose and frozen embedding to be completed on Monday) 2) can fixative be switched to sucrose at the end of Friday (tissue would remain in sucrose until frozen embedding on Monday) 3) must complete the solution switch and entire frozen embedding process on Saturday Thanks for your help. Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 29108 P-803-405-7129 F- 803-405-7474 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
