[Histonet] bone ground sections
What hematoxylin is best for doing HE on ground sections of undecalcified bone- one that won't leave a precipiate on the bone? Cindy Baranowski SJTRI Atlanta, GA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Use of EDTA to soften tissue (non - calcified)
Good morning, I'm a little stumped here - no it's not a first I know. I've come across a lab that uses EDTA powder in their ice baths to soften GI biopsies - I've never heard of this before. I've used it for bone or calcifications when nothing else was available for a surface decal and didn't like how it would sometimes affect staining. Thanks in advance for any information. Vikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Americlear Staining Problems
Good Morning Histonetters, Our HE stained slides are bleeding out the eosin. No, I did not shoot the sheriff. Called the manufacturer, and was advised to: Give more time in the Alcohols, Use only 100% Alcohols after eosin. Change the last two Alcohols and Americlear. Done. Done. Done. Stain bleeding really improved, but still shows up on hit and miss cases. Has anyone had this problem and How did you fix it Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ASCP Route 2 HT Certification
You could email them. It would not be so time consuming, and then you would have proof of an answer should anything be questioned at a later date. Good luck. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eric Velazquez Sent: Wednesday, May 04, 2011 5:52 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP Route 2 HT Certification Thank you for responding. I agree with you that the ASCP would be the best source of information, but unfortunately I spoke with someone who was in charge of reviewing the applications. She was unprofessional and offered little assistance. I will attempt to contact them again, but finding the time to be placed on hold for 30mins while I am at work is difficult. I was hoping someone would know from experience or perhaps someone who is involved with the process would be able to offer some guidance. -Eric On Wed, May 4, 2011 at 2:42 PM, Nails, Felton flna...@texaschildrens.orgwrote: Your best answer will come from ASCP so contact them directly. The number should be on the application -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eric Velazquez Sent: Wednesday, May 04, 2011 4:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ASCP Route 2 HT Certification Hi, I was hoping someone could help me with the following questions: 1) What happens to the money if I were to submit my transcript and my fees for the exam and end up not qualifying? Do I lose my money or is it credited for when I do qualify? 2) How do I determine what classes are considered BIO and Chem? Does the prefix for the course ID have to contain BIO or Chem? 3) Can the courses taken be similar? Example: School A: I took Chem10: Intro to Chemistry School B: Chm:3 Chemistry basics. If these classes are interchangeable between schools do they still count or does it have to be a more difficult chemistry course for it to qualify? 4)Is there anyway to check if you do qualify before paying exam fees? 5)Do you have to pass the class with a C or higher? I took a BIO class years ago and received a D, but I was given the credited hours for the course. Route 2 for HT certification for ASCP At least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, or an associate degree from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years. Thanks, Eric ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Bone ground sections
Hi Cindy, There is really no best hematoxylin,as any of the popular commercial formulations can be used successfully on ground sections. The key to reducing/eliminating the precipitation problem is filtering the hematoxylin really well before staining and sonicating your sections after staining. For darker nuclear staining,I like Gill III or Ehrlich's. The Celestine Blue-Hematoxylin technique also works well on ground sections. -Damien L. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Use of EDTA to soften tissue (non - calcified)
EDTA is a chelating agent whose action is to remove calcium ions from the tissue. Since GI biopsies do not contain calcium this procedure can be considered part of some Vudu practices (or snake oil medicament). René J. From: Victoria Baker bakevicto...@gmail.com To: Histo Net list server HistoNet@lists.utsouthwestern.edu Sent: Thursday, May 5, 2011 8:25 AM Subject: Re: [Histonet] Use of EDTA to soften tissue (non - calcified) Good morning, I'm a little stumped here - no it's not a first I know. I've come across a lab that uses EDTA powder in their ice baths to soften GI biopsies - I've never heard of this before. I've used it for bone or calcifications when nothing else was available for a surface decal and didn't like how it would sometimes affect staining. Thanks in advance for any information. Vikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Americlear Staining Problems
It's usually due to water on the slides. If your alcohols and Americlear are clean (water-free), check to see that your slides are totally submerged in these two reagents, and there is not water dripping down the slide. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Thursday, May 05, 2011 5:50 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Americlear Staining Problems Good Morning Histonetters, Our HE stained slides are bleeding out the eosin. No, I did not shoot the sheriff. Called the manufacturer, and was advised to: Give more time in the Alcohols, Use only 100% Alcohols after eosin. Change the last two Alcohols and Americlear. Done. Done. Done. Stain bleeding really improved, but still shows up on hit and miss cases. Has anyone had this problem and How did you fix it Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] sox 11 mantlecell lymphoma erratum
Sorry, I told you the wrong vendor of the antibody. The correct one is Acris Antibodies. http://m1.acris-antibodies.com/pdf/AP09577PU-N.pdf Hi all! Im looking for a SOX11 IHC-protocol and for feedback on this special antibody. We want to stain human FFPET and purchased the polyclonal SOX11 antibody from Acris. My protocol on Benchmark Ultra: CC1 mild, 32 min, 37°C; 1:1000 We found more positivity and nuclear staining than expected (not only in mantle cell lymphoma, but also in follikular lymphoma and diffuse large cell lymphoma). Has anyone similar experiences? Thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Her/2
marcia, was just getting ready to stain our her 2's , will let you know how they look. anita dudley providence hosp mobile, al Date: Wed, 4 May 2011 12:54:29 -0400 From: fu...@mercyhealth.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Her/2 CAP validation Her/2 slides not staining as clear as before. Is anyone else having any issues ? We use the Ventana system and our daily IHC slides are staining and controls are working well. Is there a coating on these slides from CAP ? Thanks for any help ? Marcia Funk HT,QIHC Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ANP 11630
just wondering if anyone out there would send their procedure for how they handle this. do you have a list with each type the tech can put on and if it is indirect or direct. thanks so much. and also is everyone taking their cryostats down every week and if not have you been cited? thanks anita dudley providence hosp mobile, al ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hep B
I'm looking for hepatitis B positive control tissue. Willing to trade if I have tissue that you might want. Contact me off line. Thanks. Angela Bitting, HT(ASCP), QIHC Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] NSH symposium registration
they were mailed out. Denise G Crowley dencr...@mit.edu Sent by: histonet-boun...@lists.utsouthwestern.edu 05/05/2011 10:24 AM To histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject [Histonet] NSH symposium registration Hi all, Does anyone know if we will be receiving booklets from NSH or if everything is to be done on line? Denise Crowley Histology Facility Manager Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencr...@mit.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] NSH symposium registration
Try i...@nsh.org. They'll let you and us know. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Denise G Crowley Sent: Thursday, May 05, 2011 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NSH symposium registration Hi all, Does anyone know if we will be receiving booklets from NSH or if everything is to be done on line? Denise Crowley Histology Facility Manager Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencr...@mit.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Safety glasses required for embedding
I am not sure if this has come up before, but does anyone require personnel to wear safety glasses when embedding and if so is there an OSHA policy? Thanks Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Her2 CAP survey
Cap validation Her2 slides we got one slide stained great, the other one did not, controls and cases all stained good. We use the Dako system. Jayne Halli HT,ASCP,QIHC Avera McKennan Histology 800 East 21st street Sioux Falls, SD 57117 (605)322-7142 my new e-mail address is jayne.ha...@avera.org - Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Safety glasses required for embedding
No. Rerné J. From: Cindy DeRiso cindy.der...@yale.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 5, 2011 3:11 PM Subject: Re: [Histonet] Safety glasses required for embedding I am not sure if this has come up before, but does anyone require personnel to wear safety glasses when embedding and if so is there an OSHA policy? Thanks Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Her2 CAP survey
Everyone who experiencing problems with the CAP HER2 survey slides needs to contact the CAP to document the problem and request replacement slides. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Jayne Halli jayne.ha...@avera.org 5/5/2011 3:01 PM Cap validation Her2 slides we got one slide stained great, the other one did not, controls and cases all stained good. We use the Dako system. Jayne Halli HT,ASCP,QIHC Avera McKennan Histology 800 East 21st street Sioux Falls, SD 57117 (605)322-7142 my new e-mail address is jayne.ha...@avera.org - Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Safety glasses required for embedding
Yes. Beatrice DeBrosse-Serra HT(ASCP)QIHC Investigative Pathology Scientist Pfizer Global Research Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cindy DeRiso Sent: Thursday, May 05, 2011 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety glasses required for embedding I am not sure if this has come up before, but does anyone require personnel to wear safety glasses when embedding and if so is there an OSHA policy? Thanks Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Two Questions
Been on vacation (yeah!), so am late responding. This is from the CAP Lab General Checklist. Hope it helps. GEN.71600 Fire Exit Phase II Each room larger than 1000 ft2, or in which major fire hazards exist, has at least 2 exit access doors remote from each other, one of which opens directly into an exit route. REFERENCES 1) Hoeltge GA, et al. Accidental fires in clinical laboratories. Arch Pathol Lab Med. 1993;117:1200-1204 2) National Fire Protection Association. NFPA 45: Standard on Fire Protection for Laboratories Using Chemicals . Quincy, MA: NFPA, 2004 3) National Fire Protection Association. NFPA 99: Standard for Health Care Facilities Chapter 11. Quincy, MA: NFPA, 2005 Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -- From: Daniel Schneider dlschnei...@gmail.com Sent: Wednesday, April 27, 2011 10:24 AM To: Nicole Tatum nic...@dlcjax.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Two Questions On 4/27/11, Nicole Tatum nic...@dlcjax.com wrote: 1st. Does anyone know if there is a rule or law that states a lab must have a door? I suspect that it's not explicitly spelled out, but I'd caution you, if you build a lab without doors, you have to make provisions to provide food and water for the HT's imprisoned inside. It's easier to put in doors. DLS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: thank you
Amy - Thank you for your kind words and support. The show is new and the bugs are still being sprayed. The show is actually everyone's show. I encourage anyone interested in being a guest to contact me. As you can certainly see, I have folks on who talk on a wide variety of topics. Not too much on the tech side, for sure. Just a fun late night or early Monday morning show. It's not meant to be heavy. In two weeks I will be attending my own state convention in Tampa, FL. I have a list of speakers who I would love to have as guests on the show. I don't want to mention their names and put them on the spot - Ada, Skip, Kaye, Jerry - I meant last names. LOL. I will have the studio equipment set up (Thanks again Sue Clark and the board) and be doing the interviews right there so attendees can watch and listen if they want. Anyway, thanks again Amy. I appreciate your help in getting the word out. Yours, Dave From: Amy Farnan farn...@nehealth.com To: histot...@yahoo.com Cc: litepath2...@yahoo.com Sent: Thu, May 5, 2011 1:15:16 PM Subject: thank you Hi David, Thank you very much! I have to admit I hadn't heard of your show (as I see it is new). I went online last night and looked at your page and listened to your first pod cast. I have to say its wonderful!! I am the editor of our New York State Newsletter Onstage and I will advertise your show in our next issue. I am sure others will be interested in your topics as well! Best of luck to you and thank you again, Kind Regards, Amy Farnan NYSHS Membership Secretary/Newsletter Editor/ Legislative Task Force Chair ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Americlear Staining Problems
agree...bleeding eosin is due to water on the slidesEnsure your dehydrating alcohol levels in the stainer containers are a bit higher than your Americlear (to counter evaporation during the day).if not you will get water contamination of your clearing solution. Rosie ScrimizziTechnical Specialist Siemens (Sakura)Australia Date: Thu, 5 May 2011 08:17:13 -0700 From: laurie.colb...@huntingtonhospital.com To: estellamire...@gmail.com; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Americlear Staining Problems CC: It's usually due to water on the slides. If your alcohols and Americlear are clean (water-free), check to see that your slides are totally submerged in these two reagents, and there is not water dripping down the slide. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Thursday, May 05, 2011 5:50 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Americlear Staining Problems Good Morning Histonetters, Our HE stained slides are bleeding out the eosin. No, I did not shoot the sheriff. Called the manufacturer, and was advised to: Give more time in the Alcohols, Use only 100% Alcohols after eosin. Change the last two Alcohols and Americlear. Done. Done. Done. Stain bleeding really improved, but still shows up on hit and miss cases. Has anyone had this problem and How did you fix it Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Seeking advice regarding choice of antibody clones.
Hello, I'm looking to do some IHC for APC, COX2, bax, bak, TGFbeta, TGFbeta1 and hTERT on FFPE human rectal biopsies, preferably on the Ventana Benchmark platform, and wondered if anyone had experience with these antibodies and could advise the best clones/suppliers. Regards, Mark Bromley. Manager, Histopathology. ___ Melbourne Pathology. * 103 Victoria Pde, Melbourne. | '(03) 92877806 www.mps.com.au http://www.mps.com.au/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
