[Histonet] preference: fat stains
Hello I would like your views on which provides the better result regarding fat stains. 1. fat stains (Oil red O, etc) on frozen sections 2. osmium tetroxide on formalin fixed tissue I know that osmium is nasty to work with but besides that does it produce a better result? Thanks Michelle Michelle Perrins Chief Medical Technologist Forensic Pathology Services Division Forensic Medicine Faculty Health Sciences University of Cape Town tel: +27 21 406 6001 fax: +27 21 448 1249 Email: michelle.perr...@uct.ac.za ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Safety glasses required for embedding
We went round with a Safety Dept. employee when I first started here about gloves for embedding. Couldn't convince him that we didn't need to wear them. Rene J Buesa rjbu...@yahoo.com 5/5/2011 3:46 PM No. Rerné J. From: Cindy DeRiso cindy.der...@yale.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 5, 2011 3:11 PM Subject: Re: [Histonet] Safety glasses required for embedding I am not sure if this has come up before, but does anyone require personnel to wear safety glasses when embedding and if so is there an OSHA policy? Thanks Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ASCP Route 2 HT Certification
Eric, I feel your pain. I too find it difficult to get any help from ASCP, they are happy to take your money for application fees but the customer service is definitely lacking. I have to remember to reup for my QIHC every five years because they send me no notice of it being due, and once I send in my CEU's and $$ I never hear back from them to say they received them and that I am requalified. The only way I know they got it is when the check or cc bill posts to my account. That is no way to be in my opinion. I am sure that your money for applying is an ap fee and will not be returned even if you end up not qualifying to sit for the exam. I had 3 techs get registered in the last couple of years with different levels of education. One had an associates degree with the required number of hours in chemistry and biology. One did not have the AA degree but did have some more advanced courses in chemistry and biology working towards her BS degree and the third has a BS in Biology and has had advance biology and chemistry courses. None of them had any trouble qualifying educationally, and I do not know if they required at least a C in the course, they all had to send their transcripts but they didn't have any problems. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, May 05, 2011 7:12 AM To: 'Eric Velazquez'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP Route 2 HT Certification You could email them. It would not be so time consuming, and then you would have proof of an answer should anything be questioned at a later date. Good luck. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eric Velazquez Sent: Wednesday, May 04, 2011 5:52 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] ASCP Route 2 HT Certification Thank you for responding. I agree with you that the ASCP would be the best source of information, but unfortunately I spoke with someone who was in charge of reviewing the applications. She was unprofessional and offered little assistance. I will attempt to contact them again, but finding the time to be placed on hold for 30mins while I am at work is difficult. I was hoping someone would know from experience or perhaps someone who is involved with the process would be able to offer some guidance. -Eric On Wed, May 4, 2011 at 2:42 PM, Nails, Felton flna...@texaschildrens.orgwrote: Your best answer will come from ASCP so contact them directly. The number should be on the application -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eric Velazquez Sent: Wednesday, May 04, 2011 4:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ASCP Route 2 HT Certification Hi, I was hoping someone could help me with the following questions: 1) What happens to the money if I were to submit my transcript and my fees for the exam and end up not qualifying? Do I lose my money or is it credited for when I do qualify? 2) How do I determine what classes are considered BIO and Chem? Does the prefix for the course ID have to contain BIO or Chem? 3) Can the courses taken be similar? Example: School A: I took Chem10: Intro to Chemistry School B: Chm:3 Chemistry basics. If these classes are interchangeable between schools do they still count or does it have to be a more difficult chemistry course for it to qualify? 4)Is there anyway to check if you do qualify before paying exam fees? 5)Do you have to pass the class with a C or higher? I took a BIO class years ago and received a D, but I was given the credited hours for the course. Route 2 for HT certification for ASCP At least 60 semester hours (90 quarter hours) of academic credit from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, or an associate degree from a regionally accredited college/university, with a combination of 12 semester hours (18 quarter hours) of biology and chemistry, AND one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years. Thanks, Eric ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you
RE: [Histonet] preference: fat stains
Michelle We use osmium all of the time, we find it works better for image analysis than the Oil Red O. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle Perrins Sent: Friday, May 06, 2011 7:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] preference: fat stains Hello I would like your views on which provides the better result regarding fat stains. 1. fat stains (Oil red O, etc) on frozen sections 2. osmium tetroxide on formalin fixed tissue I know that osmium is nasty to work with but besides that does it produce a better result? Thanks Michelle Michelle Perrins Chief Medical Technologist Forensic Pathology Services Division Forensic Medicine Faculty Health Sciences University of Cape Town tel: +27 21 406 6001 fax: +27 21 448 1249 Email: michelle.perr...@uct.ac.za ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: NSH Symposium/Convention Registration
Good afternoon - Paper copies of the 37th Annual Symposium/Convention Registration Brochure are currently at the press. Copies will be mailed to all NSH Members within the next two weeks. The complete program is available online through the following link. http://s3.goeshow.com/nsh/annual/2011/schedule_at_a_glance.cfm If you wish to mail your registration form, you can print the official registration form from the link below and send it to the NSH. http://s3.goeshow.com/nsh/annual/2011/PDF/SCRegForm.pdf Please feel free to call the office at 443-535-4060 or email us at hi...@nsh.org with any questions. We hope to see you in Cincinnati! Have a great weekend. Carrie Diamond Executive Director National Society for Histotechnology 10320 Little Patuxent Parkway Suite 804 Columbia, MD 21044 P: 443.535.4060 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Safety Glasses during Embedding
Please site the reference for this requirement. Thank you. Marcia Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center 1415 Ross Ave El Centro, CA 92243 760-339-7267 760-482-5365(F) www.ecrmc.orghttp://www.ecrmc.org/ Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. Â ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Safety glasses required for embedding
OSHA standard for Eye and Face Protection 29CFR1910.133 says that appropriate eye or face protection is required whenever there is a possibility of injury from: - flying particles (includes dust (for histotechs, thinks dry dye powders)) - molten metal - liquid chemicals - acid or caustic liquids - chemical gases or vapors - potentially injurious light radiation (e.g.., UV) (need UV lenses) - processes that produce aerosols of infectious agents OSHA standard for Personal Protective Equipment 29CFR1910.132 says that employers must provide and ensure employees wear PPE whenever there is exposure to hazards that can cause injury, that are: - physical - chemical - biological This could be through inhalation, absorption or physical contact. PPE include for face/head/eyes, extremities, respirators, as needed, in good condition and fit. So safety glasses fall under this standard, also. So unless you have flying particles of tissue hitting your eyes during embedding, or fine mist of infectious aerosols coming off the tissues while embedding, I would say that OSHA does not require safety glasses during embedding. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -- From: Cindy DeRiso cindy.der...@yale.edu Sent: Thursday, May 05, 2011 3:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety glasses required for embedding I am not sure if this has come up before, but does anyone require personnel to wear safety glasses when embedding and if so is there an OSHA policy? Thanks Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] removing MMA
Bernice, Let me start by first stating that it is easier to melt away the wax from a paraffin block and then go into resin (MMA), than doing it as you have asked. With that said, it has been my experience that this is very difficult to do, but somewhat possible depending upon what you wish to accomplish. The difficulty is dissolving the interior of the specimen that has been completely infiltrated/polymerized and doing so without disrupting the specimen morphology. In fact, dissolving away the exterior takes a lot of time, patience, and solvent. Additionally, you risk changes in morphology, especially on the surface because now everything is loosely supported and the bone will start to become more brittle due to drying with use of the solvent to deplastify. Now to answer your question simply, like dissolves like. If you decide to try this, I recommend to use fresh changes of 100% MMA with agitation to help dissolve away the exterior. As the solution becomes thickened from the deplastification, pour out and start again with fresh 100% MMA. You will need to repeat this process SEVERAL times. Take care not to let your dissolving attempts sit too long (overnight in small amount of solvent) or the solution will start to repolymerize. Basically, the resin will become thick in solution as it is dissolving and settle to the bottom. After a prolonged period of time the thicker layers on the bottom with start to repolymerize slowly. Therefore, before you begin I suggest that you first cut and grind away any excess resin to help you reduce both time and money with the amount of MMA solvent you will waste. A note of caution, acetone will also work but it will damage your specimen over time, so I DO NOT recommend using it. Patience and lots of 100% MMA! Regarding the 50um slides, try Sanderson's Rapid Bone Stain (Dorn and Hart Microedge) and counter with Van Gieson picrofuchsin. Let me know if you have any additional questions and feel free to call me (317-281-1975) if you would like to talk over the phone. Best Regards, Jack PS If this project is for Mahesh, tell him I said hello! :) From: b-freder...@northwestern.edu To: histonet@lists.utsouthwestern.edu Date: Fri, 6 May 2011 13:15:29 -0500 Subject: [Histonet] removing MMA We have a piece of bone in MMA. The researcher wants us to get It out and do paraffin. Is it possible? What will melt the MMA? We also have some 50um slides cut ,that they want to see blood vessels and collagen. Am I going to be able to do an EVG and trichrome or will the plastic inhibit this process? Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 mailto:b-freder...@northwestern.edu b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Safety glasses required for embedding
Flying particles can happen during embedding. I have seen molten paraffin splash someone in the eye from opening of the cassette lids. The paraffin then solidified when it hit the eye ball and the eyewash did not wash it out. They had to have it physically removed. This seems to be up for interpretation by the regs. Stacy McLaughlin, HT(ASCP) Lead Histology Technician/Laboratory Safety Cooley Dickinson Hospital 30 Locust Street Northampton, MA 01060 (413)582-2019 stacy_mclaugh...@cooley-dickinson.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Friday, May 06, 2011 1:49 PM To: cindy.der...@yale.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Safety glasses required for embedding OSHA standard for Eye and Face Protection 29CFR1910.133 says that appropriate eye or face protection is required whenever there is a possibility of injury from: - flying particles (includes dust (for histotechs, thinks dry dye powders)) - molten metal - liquid chemicals - acid or caustic liquids - chemical gases or vapors - potentially injurious light radiation (e.g.., UV) (need UV lenses) - processes that produce aerosols of infectious agents OSHA standard for Personal Protective Equipment 29CFR1910.132 says that employers must provide and ensure employees wear PPE whenever there is exposure to hazards that can cause injury, that are: - physical - chemical - biological This could be through inhalation, absorption or physical contact. PPE include for face/head/eyes, extremities, respirators, as needed, in good condition and fit. So safety glasses fall under this standard, also. So unless you have flying particles of tissue hitting your eyes during embedding, or fine mist of infectious aerosols coming off the tissues while embedding, I would say that OSHA does not require safety glasses during embedding. Peggy A. Wenk, HTL(ASCP)SLS Beaumont Hospital Royal Oak, MI 48073 -- From: Cindy DeRiso cindy.der...@yale.edu Sent: Thursday, May 05, 2011 3:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safety glasses required for embedding I am not sure if this has come up before, but does anyone require personnel to wear safety glasses when embedding and if so is there an OSHA policy? Thanks Cindy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] ASCP Route 2 HT Certification
I'll try to explain what I know, based on working with my students in my NAACLS HT and HTL programs. So I do NOT speak for ASCP BOC (Board of Certification). Don't say Well, Peggy said so. Doesn't help with my students and ASCP, so won't help with anyone else. (HISTONETTERS: If not interested in ASCP BOC, click DELETE now.) Answers to your Questions: 1. APPLICATION FEE: According to step #3 in the on-line procedure http://www.ascp.org/FunctionalNavigation/certification/GetCertified.aspx And on page 3 of the handbook http://www.ascp.org/pdf/BOR-PDFs/procedures/Examination-Procedures.aspx And on the PDF application form: http://www.ascp.org/pdf/BOR-PDFs/procedures/General-application-form.aspx The application free is non-refundable. (I'll explain why at the bottom of this, if you are interested.) 2. BIO/CHEM COURSES: ASCP will look for BIO or CHEM to see if an applicant took the right courses. But they will also look at the TITLE of the course, knowing there are other majors. Example: MLS = medical laboratory science. AH = Allied health. RT = radiation therapy. And so on - all of which have biology and chemistry courses that would be acceptable. 3. IDENTICAL COURSES: I don't know how close the courses have to be, before ASCP BOC won't count them. I would say, if you took a class at college A, then transferred to college B, and college B used that class from college A to say it was the same as one of their (college B) course, so you didn't have to take or pay for the college B course, then I would say that this one course was equal to only 1 course. even though it showed up as two different courses (after all, only one course was attended, exam taken, paid for, etc.). If you took one course at A and one course at B, and both show up as separate courses, then my feeling is that they are two different courses. (A little off to the side, the requirements for HT are 12 semester hours of biology and chemistry combined. That's 1 bio and 2 chem courses (4 credits each), or 2 bio and 1 chem course (4 credits each). NOT 12 credits of bio AND 12 credits of chem. That seems to be a common mistake.) 5. GRADE: I see nothing in the ASCP BOC HT requirements of a minimum grade point. 4. CHECKING BEFORE APPLYING: Now, as to whether there is any way to check before applying and sending in the fee - not really. I appreciate that you are trying to find out if you qualify, before you send in the application, fees and transcript. So the following is not directed towards you, personally. You had some good questions, like, do grades count (since the website doesn't say anything about it). The following is about why, in general, ASCP can't answer individual questions of applicants. Realize that thousands of people apply to the ASCP BOC for all the disciplines, each year. (In 2010, over 12,500 people took the various certification exams.) It is up to the applicant to decide, based on the criteria on-line and in the booklet and application form, if they meet all the criteria to take the exam. ASCP will NOT issue a decision over the phone or by email, before the person applies. If there is a little confusion (such as the 12 credit hours of bio/chem and what does that mean?), they will explain. But they will NOT talk about each person's transcript, work experience, etc. over the phone. They don't have the time. And until they see the transcript, they only have the person's word over the phone as to what the transcript says. And then there is the but ASCP said over the phone, when maybe they did or didn't say, or there was a misunderstanding on one or both sides. How do you prove what was verbally said? I run a NAACLS HT and HTL program, hospital based, I get lots of people calling up, wanting to know if they qualify. Yet my webpage states very clearly what the criteria is (degree, required courses, GPA, etc.), but lots of people call up, wanting exceptions. I will not talk about whether their classes meet the criteria or not. If they want this type of information, then they have to submit the application material, pay our programs' $40 application fee, and then I will look over their transcript(s) and will be willing to talk with them afterwards. Most of the time, the people who are calling know that do NOT meet the criteria, but want me spend 30 minutes or more on the phone with them, trying to talk me into reducing my requirements (not needing a degree, only having half the required courses, not having a 3.0 GPA, etc.). They already know they don't meet the criteria. But they want an exception. By my telling them that they have to submit all the required documentation first, with the application fee, before I will decide if they qualify or not, I have reduced the non-qualified people from applying. In the past, over half the applicants who applied to my programs were not qualified. But I would have to take the time to talk with them on the phone, look
Re: [Histonet] removing MMA
Years ago when I had to remove plexiglass rods from eyes, I used chloroform. It takes a few days, but methyl methacrylate dissolves in chloroform quite well. In fact, plexiglass containers for medical museum specimens are cemented together with MMA dissolved in chloroform. Bryan Llewellyn Bernice Frederick wrote: We have a piece of bone in MMA. The researcher wants us to get It out and do paraffin. Is it possible? What will melt the MMA? We also have some 50um slides cut ,that they want to see blood vessels and collagen. Am I going to be able to do an EVG and trichrome or will the plastic inhibit this process? Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 mailto:b-freder...@northwestern.edu b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] removing MMA
We use acetone to dissolve MMA from implants. Don't know what that would do to tissue. Bob On Fri, May 6, 2011 at 11:15 AM, Bernice Frederick b-freder...@northwestern.edu wrote: We have a piece of bone in MMA. The researcher wants us to get It out and do paraffin. Is it possible? What will melt the MMA? We also have some 50um slides cut ,that they want to see blood vessels and collagen. Am I going to be able to do an EVG and trichrome or will the plastic inhibit this process? Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 mailto:b-freder...@northwestern.edu b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Removing MMA
Hi Bernice, If you have any intentions of putting this sample on an enclosed tissue processor for paraffin infiltration, after methacrylate removal, you must remove every trace of methyl methacrylate monomer. Traces (even small traces) of methacrylate monomer can wreck havoc on most enclosed tissue processor solvent-transfer lines and pump gaskets; you could end up with a very expensive repair bill. For transferring into paraffin, you’re better off treating these samples as you would treat a section you wish to deplasticize. Assuming your sample was properly fixed and processed from the beginning, xylene or toluene will do the job of breaking down the polymerized methacrylate just fine. Once the trimmed-down block has dissolved down, several more changes are required to remove the methacrylate and avoid potential carryover danger. As an additional safeguard, I would take the sample back to 200 proof ethanol or 100% reagent ethanol for a couple of changes then start the paraffin infiltration process with the last ethanol step on your processor. If you need to decalcify this sample, then you can just continue on back to water and start from the beginning. Yes, you can do any tinctorial stain on your ground sections if etched well beforehand. -Damien L. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] hematoxylin washed off
Hello Every body, We changed the solutions testerday in the processor, and today Dr. complained about pale hematoxylin. Can u suggest what is wrong. thanks. anu. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
