Re: [Histonet] (no subject)
could they possibly mean a H pylori? associated with gastric ulcers On Tue, May 10, 2011 at 3:47 PM, Rene J Buesa rjbu...@yahoo.com wrote: The so called reflux is a symptom of a gastric condition and, as such, cannot be detected by HC or IHC. Perhaps a gastric biopsy could reflect an anatomical alteration that accompanies it but cannot measure. Have your pathologists seriously consider this issue? René J. From: Dorothy Glass techman...@yahoo.com To: Histonet@lists.utsouthwestern.edu Sent: Monday, May 9, 2011 8:50 PM Subject: Re: [Histonet] (no subject) What is an IHC stain for gastric reflux. Pathologists are wanting to start using the procedure. Can anyone help? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) There are nights when the wolves are silent and only the moon howls. George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] stainless steel cassettes
Hi , Does anyone have any stainless steel cassettes they would like to sell or does anyone know where we could purchase them? As always, thank you for your help and happy hump day! Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Peloris protocols
Hi everyone, I am looking for anyone who is using protocols for the xylene free or xylene substitues on the Peloris tissue processor in the Florida area. Would anyone be willing to share them with me, as well as any validation protcols for Her2? You can reply back at this address. Thanks in advance for your help. Kim Tournear - HT, QIHC (ASCP) Field Support Specialist Core Histology - Southeast Leica Microsystems Inc. ~Don't be afraid your life will end, be afraid it will never begin~ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC run print outs
Does everyone print out IHC run reports after each run? If so, how long do you keep them and are the pathologists supposed to sign off on them with their slides? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC run print outs
To meet CAP requirements, we print un reports and send send to the pathologists with slides, they review, sign off and we keep for 2 yrs. All QC paperwork is kept for 2 yrs. William DeSalvo, B.S., HTL(ASCP) Date: Wed, 11 May 2011 10:01:03 -0500 From: amber.mcken...@gastrodocs.net To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC run print outs Does everyone print out IHC run reports after each run? If so, how long do you keep them and are the pathologists supposed to sign off on them with their slides? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC run print outs
Same here Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, May 11, 2011 11:09 To: amber.mcken...@gastrodocs.net; histonet Subject: RE: [Histonet] IHC run print outs To meet CAP requirements, we print un reports and send send to the pathologists with slides, they review, sign off and we keep for 2 yrs. All QC paperwork is kept for 2 yrs. William DeSalvo, B.S., HTL(ASCP) Date: Wed, 11 May 2011 10:01:03 -0500 From: amber.mcken...@gastrodocs.net To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC run print outs Does everyone print out IHC run reports after each run? If so, how long do you keep them and are the pathologists supposed to sign off on them with their slides? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC run print outs
Only when I received out automaton from DAKO to make sure that everything was done correctly. I stopped doing that after all the protocols were validated. It was an extra step that just added more time consuming unnecessary documentation. René J. From: Amber McKenzie amber.mcken...@gastrodocs.net To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 11, 2011 11:01 AM Subject: [Histonet] IHC run print outs Does everyone print out IHC run reports after each run? If so, how long do you keep them and are the pathologists supposed to sign off on them with their slides? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Her2 CAP survey
Our staining was also light. I called CAP and they said to leave the result area blank, mark the bubble for unable to analyze (11), and to write an explanation on the last page. My pathologist is questioning why we wouldn't mark specimen unsatisfactory (33). What are others that had light staining doing and/or what have you been told by CAP? CAP also told me that they didn't have any replacement slides to send out. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, May 05, 2011 1:06 PM To: Jayne Halli; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Her2 CAP survey Everyone who experiencing problems with the CAP HER2 survey slides needs to contact the CAP to document the problem and request replacement slides. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Jayne Halli jayne.ha...@avera.org 5/5/2011 3:01 PM Cap validation Her2 slides we got one slide stained great, the other one did not, controls and cases all stained good. We use the Dako system. Jayne Halli HT,ASCP,QIHC Avera McKennan Histology 800 East 21st street Sioux Falls, SD 57117 (605)322-7142 my new e-mail address is jayne.ha...@avera.org - Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IHC run print outs
We currently do. And we have the Dako system. So everytime we print the IHC reports it takes pages and pages. The pathologist are supposed to sign off on them and return them to me. I am then supposed to file them. But... The major problems are it takes up hundreds of sheets of paper each week. they do not sign them do not return them or return a few months worth of reports all at once we don't have time to file them so that we can retrieve them if needed It was done this way when I got here and I have been trying to figure out a way to stop it. I am not sure why we need them. If the stains are mentioned in the report then we shouldn't need them right? The one thing that is stopping us is that after staining when we organize the slides to hand out, we look at the IHC report to see what pathologist to give them to and how many slides there needs to be. Which is handy when you have over a hundred slides to hand out daily I have asked Dako to add a simplier IHC report to the software. A one page per case report maybe, with the pathologist name and the number of slides stained. This would cut down on the paperwork and make it a lot easier to file and retrieve. Maybe in the next software upgrade...I am hoping If anyone has any ideas please let me know. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie [amber.mcken...@gastrodocs.net] Sent: Wednesday, May 11, 2011 11:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC run print outs Does everyone print out IHC run reports after each run? If so, how long do you keep them and are the pathologists supposed to sign off on them with their slides? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Her2 CAP survey
Laurie, when we called CAP they also told us to use Code 11. That's a GOOD point - specimen unsatisfactory is this issue. Dana Settembre -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, May 11, 2011 11:16 AM To: Richard Cartun; Jayne Halli; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Her2 CAP survey Our staining was also light. I called CAP and they said to leave the result area blank, mark the bubble for unable to analyze (11), and to write an explanation on the last page. My pathologist is questioning why we wouldn't mark specimen unsatisfactory (33). What are others that had light staining doing and/or what have you been told by CAP? CAP also told me that they didn't have any replacement slides to send out. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, May 05, 2011 1:06 PM To: Jayne Halli; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Her2 CAP survey Everyone who experiencing problems with the CAP HER2 survey slides needs to contact the CAP to document the problem and request replacement slides. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Jayne Halli jayne.ha...@avera.org 5/5/2011 3:01 PM Cap validation Her2 slides we got one slide stained great, the other one did not, controls and cases all stained good. We use the Dako system. Jayne Halli HT,ASCP,QIHC Avera McKennan Histology 800 East 21st street Sioux Falls, SD 57117 (605)322-7142 my new e-mail address is jayne.ha...@avera.org - Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC run print outs
We had our in-house IT person create a print-out for us. We are a private lab so we are not inspected by CAP. That being said NYSDOH requires much of the same QC paperwork so we make do with what we have. On Wed, May 11, 2011 at 11:09 AM, WILLIAM DESALVO wdesalvo@hotmail.comwrote: To meet CAP requirements, we print un reports and send send to the pathologists with slides, they review, sign off and we keep for 2 yrs. All QC paperwork is kept for 2 yrs. William DeSalvo, B.S., HTL(ASCP) Date: Wed, 11 May 2011 10:01:03 -0500 From: amber.mcken...@gastrodocs.net To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC run print outs Does everyone print out IHC run reports after each run? If so, how long do you keep them and are the pathologists supposed to sign off on them with their slides? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] IHC run print outs
We have the Ventana Ultra Software. I don't print the reports, but save them on an extra file on the pc as pdfs. So in case of a software-breakdown, I can search the reports. It's just a QC-storage. I don't see much sense in printouts, because pathologists usually don't do troubleshooting and have no interest in lotnumbers. This is our job and we need then the information, if the machine had dispensed the right reagens. The doctors get the printed order with patient-label with the slides. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Amber McKenzie Gesendet: Mittwoch, 11. Mai 2011 17:01 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] IHC run print outs Does everyone print out IHC run reports after each run? If so, how long do you keep them and are the pathologists supposed to sign off on them with their slides? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Her2 CAP survey
When I called CAP they told me to record the results I got as long as my controls stained properly...and we did. Jayne Halli HT,ASCP,QIHC Avera McKennan Histology 800 East 21st street Sioux Falls, SD 57117 (605)322-7142 my new e-mail address is jayne.ha...@avera.org -Original Message- From: Settembre, Dana [mailto:sette...@umdnj.edu] Sent: Wednesday, May 11, 2011 10:23 AM To: 'Laurie Colbert'; Richard Cartun; Jayne Halli; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Her2 CAP survey Laurie, when we called CAP they also told us to use Code 11. That's a GOOD point - specimen unsatisfactory is this issue. Dana Settembre -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, May 11, 2011 11:16 AM To: Richard Cartun; Jayne Halli; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Her2 CAP survey Our staining was also light. I called CAP and they said to leave the result area blank, mark the bubble for unable to analyze (11), and to write an explanation on the last page. My pathologist is questioning why we wouldn't mark specimen unsatisfactory (33). What are others that had light staining doing and/or what have you been told by CAP? CAP also told me that they didn't have any replacement slides to send out. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, May 05, 2011 1:06 PM To: Jayne Halli; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Her2 CAP survey Everyone who experiencing problems with the CAP HER2 survey slides needs to contact the CAP to document the problem and request replacement slides. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Jayne Halli jayne.ha...@avera.org 5/5/2011 3:01 PM Cap validation Her2 slides we got one slide stained great, the other one did not, controls and cases all stained good. We use the Dako system. Jayne Halli HT,ASCP,QIHC Avera McKennan Histology 800 East 21st street Sioux Falls, SD 57117 (605)322-7142 my new e-mail address is jayne.ha...@avera.org - Avera is a health ministry rooted in the Gospel. Our mission is to make a positive impact in the lives and health of persons and communities by providing quality services guided by Christian values. Avera is sponsored by the Benedictine and Presentation Sisters. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Annexin V
Histonetters: Looking for Annexin V Ab porcine reactiveanybody out there used Annexin V on piggies for histology? What is the scoop? I'd rather not re-invent the wheel. I am sure, someone has done ths.CB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for Mike Riechenbach
Long lost friend looking for Mike. He knows me as either Kim Mann or Kim Saunders from Tucson. Would love to here from him. Sent from the iPhone of Kim Tournear ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Opening for Histotech/Pathology Lab Manager (Monterey, CA)
See job description below. If you want more info before applying, you can contact me or Helayne (her email is the send resume to email below). Thanks, Patty *Histotech Opening in Monterey, CA* *Position Title*: Histotech/Pathology Laboratory Manager *Location*: Physicians Office (GI) Pathology Laboratory in Monterey, CA *Shift*: Full Time, M – F. Hours are somewhat flexible and must coordinate with pathologists’ schedules. *Job Details:* We are looking for an experienced Histology Tech, who will be accountable for direct supervision and operation of our single-tech histology laboratory, and maintain high quality and cost-effective services. This position requires someone who is reliable, hard working, able to work independently and exhibits a strong attention to detail. The Histology tech will perform all duties related to the preparation of surgical GI specimens to be examined by pathologists. This includes but is not limited to:** · Tissue Processing (microwave) · Embedding · Microtomy · HE (automated) and Special Staining (manual) · Cover-slipping (manual) Also performs all duties related to laboratory management, including equipment care and maintenance, inventory/ordering, archiving of specimens, recordkeeping, etc. *We are a formalin-free and xylene-free laboratory. *Job Requirements:* Ability to work with no supervision HT or HTL (ASCP) certification required At least 3 years experience working in histology with little or no supervision Available to start in June 2011 *Compensation: * Competitive salary, health benefits and 401(k) *To apply*: *Please submit resume t*o hwilli...@montereygi.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DMSO in Tissue processor
Hi Everyone, I was hoping that I could ask a question about the use of paraffins with DMSO in tissue processors. I was wondering if there are any tissue processors that recommend or specify that paraffins containing DMSO not be used. I am not aware of any of the older versions but just wondered about the newer microwave processors and other newer versions of processors now on the market. Thanks for all your help! Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010 x229 | Fax: 972-436-1369 dsi...@statlab.commailto:maub...@statlab.com | www.statlab.comhttp://www.statlab.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] hematoxylin and DAB/Alkaline Phosphatase staining
Dear Histonet Members, I am currently staining 20 um cryostat sections of brain tissue and visualizing with DAB along with hematoxylin counterstain. For sections stained with the same concentration of primary antibody, I seem to get lighter DAB staining for slides with hematoxylin than ones without. Is it possible that hematoxylin can interfere with DAB staining? I am using the ABC kit from for DAB and pre-made Hematoxylin QS, both from Vector Labs. On a different note, I've noticed some sponginess in occasional sets of slides. I'm wondering what can be the cause. A labmate told me that it's probably something wrong with the fixation or cryoprotection. I've treated the brain tissue the same for the batch, and only one slide set had sponginess. I fix in 4% PFA for 24 hours, cryoprotect with 30% sucrose for 1-5 days, and mount in OCT. Any suggestions would be appreciated. Thank you for your help. Sincerely, Cheuk ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
