[Histonet] Recycled Xylene
Hello in Histo land. I know it is a subject brought up over and over again but I need to get the opinion of my fellow Histo techs on processing tissue with recycled Xylene. Yes I know it saves money and is better for the earth, but is the quality of the tissue the same??? Coverslipping and clearing slides with it I can see being ok, but processing with it??? It is not 100% after recycling. I could use any thought on the subject. Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thank you
To all the Companies and Private labs that offered a microtome for sale. We have chosen one company to do business. I am very thankful for the great response we received. Sincerely. Lin Bustamante. Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate Histology Lab Supervisor Veterinary Integrative Bioscience Texas AM University College Station, TX 77843-4458 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: p63
We get ours from Ventana. Sheila Fonner, HT (ASCP) KDL Pathology Knoxville, TN -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne Sent: Monday, May 23, 2011 1:46 PM To: 'Joanne Clark'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: p63 I believe the only place you can buy p63 is through Biocare, www.biocare.net. Lynne Bell, HT (ASCP) Histology Team Leader 802-371-4923 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Recycled Xylene
We process with recycled xylene and have no problems. We also use it for staining but use fresh for coverslipping. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Wednesday, May 25, 2011 6:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled Xylene Hello in Histo land. I know it is a subject brought up over and over again but I need to get the opinion of my fellow Histo techs on processing tissue with recycled Xylene. Yes I know it saves money and is better for the earth, but is the quality of the tissue the same??? Coverslipping and clearing slides with it I can see being ok, but processing with it??? It is not 100% after recycling. I could use any thought on the subject. Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Recycled Xylene
We recycle it and have had no problems with processing, no complaints from the docs. Curt -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marshall, Kimberly K Sent: Wednesday, May 25, 2011 6:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled Xylene Hello in Histo land. I know it is a subject brought up over and over again but I need to get the opinion of my fellow Histo techs on processing tissue with recycled Xylene. Yes I know it saves money and is better for the earth, but is the quality of the tissue the same??? Coverslipping and clearing slides with it I can see being ok, but processing with it??? It is not 100% after recycling. I could use any thought on the subject. Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] pathology software
We are considering a change in software LIS. We are a typical small private path lab, currently path and cyto only but are looking at adding clinical in the future. Need to have the EMR update capability, I think it's an HL7 interface, to transfer results electronically and upload directly to a clients EMR. What are you all using in your labs? I can't go with some of the big guys like Cerner, though it is nice and top of the line, it's a little out of my budget. I need to have the ability to have several different label formats, some of our hospital clients like labels with little info, some like more info. So I want to be able to modify a slide label based on each clients desire and then save it to the database as specific for that dr. Anyone out there have a product that you're just over the top impressed with, produce and customer service combined? Thanks, Curt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recycled Xylene
If you are using a good cracking recycling instrument the recycled xylene = 100% xylene and there cannot be any differences in behavior against pure-unused-mew xylene. That is what I always found for more than 15 years. René J. From: Marshall, Kimberly K kkmarsh...@anthc.org To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 25, 2011 9:20 AM Subject: [Histonet] Recycled Xylene Hello in Histo land. I know it is a subject brought up over and over again but I need to get the opinion of my fellow Histo techs on processing tissue with recycled Xylene. Yes I know it saves money and is better for the earth, but is the quality of the tissue the same??? Coverslipping and clearing slides with it I can see being ok, but processing with it??? It is not 100% after recycling. I could use any thought on the subject. Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dayton Ohio Histotech Needed
Hey All A fulltime Histotech position is open at Dayton Children's Hospital in Dayton Ohio. We are a small hospital. We have one part time, one full time (that could be you) and myself. We process about 5000 cases a year, we average about 15-40 blocks a day. This is Dayton Children's Hospital, good benefits, not a whole lot of stress. If you're looking for a great place with a great group of people give me a call, or call HR at 937-641-8090 and ask for Dan Krauss. Or just apply online at http://www.childrensdayton.org/cms/careers/index.html If you want more specifics you can call me at 641-3000 ext 8229. Please no Headhunters, we are not allowed to use employment agencies, thanks. Matt Chase Supervisor of Pathology NOTICE: The information contained in this e-mail and any accompanying documents or files is intended for the sole use of the recipient to whom it is addressed, and may contain information that is privileged, confidential, and prohibited from disclosure under applicable law. If you are not the intended recipient, or authorized to receive this on behalf of the recipient, you are hereby notified that any review, use, disclosure, copying, or distribution is prohibited and possibly a violation of federal/state law or regulations. If you received this information in error, please notify The Children's Medical Center of Dayton immediately via telephone at (937) 641-5293, or via electronic mail cmcconfidential...@childrensdayton.org and promptly destroy the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recycled Xylene
Very true because if you notice the label on a purchased bottle of xylene it says Xylenes. Your recycled product should be pure xylene and thus a higher purity than what you started with. Our lab has been recycling since the mid '90's. We no longer process with xylene but we still have it in the lab for various things. Hope this helps. On Wed, May 25, 2011 at 11:11 AM, Rene J Buesa rjbu...@yahoo.com wrote: If you are using a good cracking recycling instrument the recycled xylene = 100% xylene and there cannot be any differences in behavior against pure-unused-mew xylene. That is what I always found for more than 15 years. René J. From: Marshall, Kimberly K kkmarsh...@anthc.org To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 25, 2011 9:20 AM Subject: [Histonet] Recycled Xylene Hello in Histo land. I know it is a subject brought up over and over again but I need to get the opinion of my fellow Histo techs on processing tissue with recycled Xylene. Yes I know it saves money and is better for the earth, but is the quality of the tissue the same??? Coverslipping and clearing slides with it I can see being ok, but processing with it??? It is not 100% after recycling. I could use any thought on the subject. Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recycled Xylene
The word xylenes in a pure xylene bottle means it contains a mixture of ORTHO-; META-; and PARA-xylene (3 different xylene molecular configurations), hence the title xylenes. After you distill your used xylene, you will probably end with a very similar proportion of the 3 molecules and it will be xylenes also. René J. From: Jennifer Campbell campbe...@muhlbauerlab.com To: Rene J Buesa rjbu...@yahoo.com Cc: Marshall, Kimberly K kkmarsh...@anthc.org; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, May 25, 2011 11:38 AM Subject: Re: [Histonet] Recycled Xylene Very true because if you notice the label on a purchased bottle of xylene it says Xylenes. Your recycled product should be pure xylene and thus a higher purity than what you started with. Our lab has been recycling since the mid '90's. We no longer process with xylene but we still have it in the lab for various things. Hope this helps. On Wed, May 25, 2011 at 11:11 AM, Rene J Buesa rjbu...@yahoo.com wrote: If you are using a good cracking recycling instrument the recycled xylene = 100% xylene and there cannot be any differences in behavior against pure-unused-mew xylene. That is what I always found for more than 15 years. René J. From: Marshall, Kimberly K kkmarsh...@anthc.org To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 25, 2011 9:20 AM Subject: [Histonet] Recycled Xylene Hello in Histo land. I know it is a subject brought up over and over again but I need to get the opinion of my fellow Histo techs on processing tissue with recycled Xylene. Yes I know it saves money and is better for the earth, but is the quality of the tissue the same??? Coverslipping and clearing slides with it I can see being ok, but processing with it??? It is not 100% after recycling. I could use any thought on the subject. Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histology Technologist 2 Job Opening at Northwestern University, Chicago Campus
Hi Fellow Histonetters, The ad I posted is not from a job placement agency. Please follow the Northwestern University HR link and navigation instructions I posted in the original ad to fill out the job application and send your resume. Thanks, Donna Donna J. Emge, ASCP-HT Mouse Histology and Phenotyping Laboratory Manager Northwestern University Olson Pavilion 8-333 710 North Fairbanks Court Chicago, IL 60611 d-e...@northwestern.edu 312-503-2679 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Unlabeled specimens
What are your procedures for rejection and correction when you receive either a surgical or Nongyn specimen that has incomplete information on the requisitions or the container is not labeled properly? Do you send it back to the provider's office? Have them come to your lab to make the corrections? Throw away the specimen? Tom THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Unlabeled specimens
Hi Tom, Consider how long it would take to send it back to get a response. Some clients may be far away. If that Non-gyn needs to be processed, we would call them, document the information and contacts, and politely remind them to fill out the forms. Yes, this can be time consuming. In these days of barcoding and specimen tracking, nothing is thrown out, and the majority of rejected specimens get sent back to the physician/group. Sometimes they don't come back, but that's their prerogative. Hugh-Hawaii From: Podawiltz, Thomas tpodawi...@lrgh.org To: 'Histonet@lists.utsouthwestern.edu' Histonet@lists.utsouthwestern.edu Sent: Wednesday, May 25, 2011 1:34 PM Subject: [Histonet] Unlabeled specimens What are your procedures for rejection and correction when you receive either a surgical or Nongyn specimen that has incomplete information on the requisitions or the container is not labeled properly? Do you send it back to the provider's office? Have them come to your lab to make the corrections? Throw away the specimen? Tom THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Diane Tokugawa/CA/KAIPERM is out of the office.
I will be out of the office starting 05/24/2011 and will not return until 05/31/2011. Note: For Cytology issues, please call Molly (day) at 8-421-5487 or Eric (eve) at 8-421-5405, For Histology issues, please call the general histology lab 8-421- 5408, Mario 8-421-4961 (day), Kiran 8-421-5404 (late afternoon/eve) or Wanda Lau for Cyto/Histo issues 8-421-5426.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] bone marrow biopsy
Hi, I have pasted the procedure below. Please be aware that this comes with the caveat that it is a very slow process compared to the strong acid solutions that we are most accustomed to. If there is a diagnostic need to expedite the results, then this may not be the best option. Incidentally just so no one thinks I'm wise enough to come up with this on my own, here is the reference (with original references at the end of that link). http://www.ihcworld.com/_protocols/histology/decalcification_solution.htm I am wise enough to give credit where it is due :-) Good luck, Amos 10% EDTA (pH7.4), or 10% EDTA/TRIS-HCl (pH 7.4), or 10% EDTA with 0.07% (w/v) Glycerol (pH 7.4) * * EDTA is a chelating agent, and it can be made 10% solution with distilled water, pH 7.4. This is also the preferred solution for decalcifying bone material for transmission electron microscopy. Specimens can be decalcified in this solution over several days up to several weeks in a refrigerator at 4 C, depending on degree of mineralization and size of specimen. The fresh solution is changed several times or once a week. After decalcification, samples can be routinely processed and embedded in paraffin. Several studies have shown that EDTA decalcified bone material preserves DNA better, and preferable for ISH analysis, and TUNEL staining. It is also suitable for most of immunohistochemical staining protocols. Message: 12 Date: Wed, 25 May 2011 12:26:52 + From: Diana Martinez-Longoria dmlongo...@ecrmc.org Subject: [Histonet] bone marrow biopsy To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID: 12b71261212be94bb9fb7735484b9fa1014...@exmbx01.ecrmc.ci.el-centro.ca.us Content-Type: text/plain; charset=us-ascii Hello Histoland, I read on Histonet that some of you guys use EDTA for bone marrow core bx's. I was wondering what is the protocol? It will help us a lot. Thanks in advance! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] validating the Leica Peloris
Our lab is in the process of validating the Leica Peloris II tissue processor, is there anyone out there who has the Peloris II? How did you validate? how many runs, and what type of tissue did you use? Thank you for your in put. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Available for sale
(1) Mopec MB100 Grossing Station http://media2.mopec.com/media/pdf/MBSeriesGrossingStationBrochure.pdf excellent condition Mark Sofferman, President American ReSource Medical 324 West Englewood Avenue Teaneck, NJ 07666 P: 201.833.1550 F: 201.833.1575 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
