RE: [Histonet] MSDS info
Your suppliers are required by law to supply msds and they would no doubt be please to do so. However the Sigma website is great as you can search all their reagents and click on MSDS and download a PDF file of the MSDS. http://www.sigmaaldrich.com/ This hyperlink should take you to their site. Regards Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dianne E. Holmes Sent: 27 May 2011 17:48 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] MSDS info HELP !!! I am having to list all my chemicals in the lab for the Safety dept. Several have no MSDS sheet with them? (been here longer than I have!) Where can I obtain this info to put in my records? Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] a Perfect Red
Dear All, Thank you all for your contributions to my request for information about A Perfect Red, Yes I should have searched it on the Net. Just didn't think! Anyway I shall be ordering it and will enjoy reading it. Thank you all a million. Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] back up power for VIPs?
Hello Sheila, We are using the Smart-UPS 2200 from APC. We are also on a dedicated emergency line through our facility. We are running the Sakura VIP 5. I cannot give you any other specifics about the UPS since I can't get to the back of it but it was recommended and installed by our biomed people. Seems to work fine. That model has been discontinued but this is the recommended replacement... APC Smart-UPS 2200VA USB Serial 120V More Images APC Smart-UPS, 1980 Watts / 2200 VA,Input 120V / Output 120V, Interface Port DB-9 RS-232, SmartSlot Includes: CD with software, Smart http://www.apc.com/products/resource/include/techspec_index.cfm?base_sku=SUA2200 Hope this helps... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Friday, May 27, 2011 9:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back up power for VIPs? Hello everyone: We are experiencing lots of quick power outages at work that keeps setting the processors into power outage alarms. Can anyone recommend a back up power product. I was told we can't put them on battery back up b/c of their power requirements??? They are on emergency power. Thanks for your help in advance. :) Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histologic Technician 2 Job Opening at University of Washington
Histologic Technician 2 Job Requisition 72777 University of Washington Department of Comparative Medicine The department's Histology and Imaging Core is currently seeking a full-time temporary employee for a histologic technician 2 position. The candidate will be working as part of a close-knit team supporting research projects from various sources within and external to the university. You can access the University of Washington Careers site by going to: http://www.washington.edu/admin/hr/jobs/apl/index.html If you are an Internal Applicant click the UW Employee Login link. If you are an External Applicant click the External Applicant Login link. Type 72777 in the Req # box to reach the full job description. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Help with Masson's Trichrome - not working on cryo sections
Hello, I am completely stumped by a problem we¹ve been having with our Masson¹s Trichrome. It isn¹t working properly on cryo sections. We developed our protocol several years ago and it has been a standard in our lab without any trouble, until about four months ago when it just stopped working on frozen sections. It still works fine on paraffin sections. You can see images at this link: http://www.med.umn.edu/lhi/prod/groups/med/@pub/@med/@dom/@lhi/documents/con tent/med_content_340494.pdf What seems to be happening is that the beibrich scarlet + acid fuchsin reagent is either not staining the muscle tissue, or is washing out of the tissue. The aniline blue is still staining the connective tissue very well (it stopped working, too, but we solved by reducing time in water rinse following stain). My Masson¹s protocol is very standard essentially straight out of the Armed Forces Institute of Pathology Manual (p. 94). I fix my cryosections in 10% NBF x 30 mins. before starting the protocol. I¹ve made sure my formalin is fresh and properly stored, with no white precipitate. I¹ve tried the Bouin¹s mordant step at both 56C and room temp overnight. I¹ve reduced my distilled water rinses following the biebrich scarlet and aniline blue steps to just a quick dip, thinking maybe I was de-staining too much before setting the stains with acetic acid. I¹ve re-made all solutions, checked all components and pH levels. I haven¹t done anything different with my frozen tissues, and I¹ve tried several different freshly cut tissues. Has anyone seen this before and have any tips on what is going wrong? Your help is greatly appreciated! -- Jennifer L. Fricton, B.S. Scientist, Lab Manager Lillehei Heart Institute Histology Microscopy Core Facility University of Minnesota School of Medicine, Division of Cardiology 312 Church Street SE 4-290 Nils Hasselmo Hall Minneapolis, MN 55455 Lab: 612-626-3090 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HOT New Histology Opportunity in Baltimore and DC!!
Histonet, We are currently searching for a histology laboratory professional who would be interested in working in the Field for a global manufacturer of Histology and IHC equipment. The position offers a very strong Base Salary + Bonus + Car Allowance + Full Benefits. This position does not require previous field experience and is perfect for a histotech who is interested in opportunities outside of the lab. Please contact me directly at m...@personifysearch.com to learn more. Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com *Field Support Specialist* *The Company:* Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Microscopy, Confocal Laser Scanning Microscopy, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. *Primary Responsibilities:* The primary responsibility of this role will be to provide in-field technical applications support for current and next generation range of products. The FSS will install/set-up instrumentation in customer laboratories, perform demonstrations and maintain demonstration equipment in a clean and operational manner. The FSS will also train customers on the use of instrumentation. Conducting in-field post purchase applications support and performing instrument validations will be a key responsibility of this position. Additional Responsibilities: - Build an in-depth understanding of new product technologies and their applications in a diagnostic environment - Contribute to the strategic planning process by providing technical data on instrumentation and reagents - Conduct post-purchase reagent order management - Be the customer liaison by providing scientific and laboratory expertise for in-field installations, evaluations, training and trouble-shooting - Provide customer support for remote problem solving - Design and perform experiments to investigate and solve tough technical applications problems ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] INFAR-1 KGF antibodies
Hello, I am looking for an antibody to use for INFAR-1 (interferon alpha receptor) to use on fresh frozen mouse tissue sections. If anyone can recommend any antibodies and the fixation used it would be much appreciated. Best regards, James ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re-using modified Davidson's solution
Hi All, Can one re-use modified Davidson's solution for fixing eyes and testes, or has it lost its potency/efficacy after being used previously? Can you rejuvenate it by adding more acetic acid? (how much?) I appreciate any helpful comments. Thank you, Robin Robin R. Dean, Ph.D. Senior Scientist Study Director Comparative Biosciences, Inc. 786 Lucerne Dr. Sunnyvale, CA (408) 738-8060 robin_d...@compbio.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Help with Masson's Trichrome - not working on cryo sections
Jennifer, Looking at the images, is it possible that the slides were air-dried for too long prior to staining? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Fricton Sent: Wednesday, 1 June 2011 2:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help with Masson's Trichrome - not working on cryo sections Hello, I am completely stumped by a problem we¹ve been having with our Masson¹s Trichrome. It isn¹t working properly on cryo sections. We developed our protocol several years ago and it has been a standard in our lab without any trouble, until about four months ago when it just stopped working on frozen sections. It still works fine on paraffin sections. You can see images at this link: http://www.med.umn.edu/lhi/prod/groups/med/@pub/@med/@dom/@lhi/documents/con tent/med_content_340494.pdf What seems to be happening is that the beibrich scarlet + acid fuchsin reagent is either not staining the muscle tissue, or is washing out of the tissue. The aniline blue is still staining the connective tissue very well (it stopped working, too, but we solved by reducing time in water rinse following stain). My Masson¹s protocol is very standard essentially straight out of the Armed Forces Institute of Pathology Manual (p. 94). I fix my cryosections in 10% NBF x 30 mins. before starting the protocol. I¹ve made sure my formalin is fresh and properly stored, with no white precipitate. I¹ve tried the Bouin¹s mordant step at both 56C and room temp overnight. I¹ve reduced my distilled water rinses following the biebrich scarlet and aniline blue steps to just a quick dip, thinking maybe I was de-staining too much before setting the stains with acetic acid. I¹ve re-made all solutions, checked all components and pH levels. I haven¹t done anything different with my frozen tissues, and I¹ve tried several different freshly cut tissues. Has anyone seen this before and have any tips on what is going wrong? Your help is greatly appreciated! -- Jennifer L. Fricton, B.S. Scientist, Lab Manager Lillehei Heart Institute Histology Microscopy Core Facility University of Minnesota School of Medicine, Division of Cardiology 312 Church Street SE 4-290 Nils Hasselmo Hall Minneapolis, MN 55455 Lab: 612-626-3090 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
