[Histonet] AFB stains, shiny water artifact, in resource limited countries
Hi, I am volunteering in a 300 bed Kenya hospital for a month (Kijabe), and we have a problem with numerous shiny red artifacts, some of which are the shape and size of TB (which are very distracting), apparently caused by processing with 70% alcohol. Does anyone know a way to eliminate these while still using 70% isopropryl, which is apparently the only economically feasible processing reagent available here? Would it help to soak the blocks in 95% before cutting (we have small amounts of 95% available). Thanks very much in advance! Alice Alice Neumann MD Western Wyoming Pathology PC Pinnacle Pathology PC 9423 West Kentucky Place Lakewood, CO 80226 Phone: 303-989-3098 24 Hour Cell Phone: 307-413-4092 alineum...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Grimelius stain on gut + Chromogranin
Hi All, Just wanted to know if anyone had any suggestions on how I could improve the sensitivity of this stain as my last effort was very weak, also does anyone have any good methods for a chromogranin on mouse tissue. Kind Regards Sara - Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that we can arrange for proper delivery, and then please delete the message from your inbox. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PRN Needed in Little Rock
Good Morning, University of Arkansas for Medical Sciences is looking for a PRN Histologist (must be HT registered) for days with an occasional possible night shift. Please contact me with your information to be considered. E-mail is preferred as I am rarely in my office. This will be an ongoing position on an as needed basis for a minimum of one year. Best Regards, Pamela A Marcum AP Histology/Gross Room Supervisor 4301 W Markham Street Little Rock AR Office: 501-686-5341 Fax: 501-686-7151 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica CV 5030
Micromount (Surgipath- now a part of Leica). 21 gauge needle. No problem. Make sure volume set is correct for the media. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 09, 2011 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica CV 5030 Anyone using the Lieca CV 5030, what mounting medium and what size needle are you using? Thanks, Linda Our Vision: To be the #1 choice for all your GI services Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 396-2623 Email: lbla...@digestivespecialists.commailto:lbla...@digestivespecialists.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] question on H pylori
We do either IHC or a Modified Steiner, depending on the Pathologist's preference. We switched to Newcomer Supply's Steiner-Chapman Modified Silver Stain Kit about a year ago, because it eliminated the use of Uranyl Nitrate. We have been delighted with the consistency of the stain. Sandy Harrison, HTL (ASCP) Anatomical and Surgical Pathology Supervisor, VAMC Minneapolis 612-467-2449 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa Sent: Tuesday, June 07, 2011 1:12 PM To: histonet@lists.utsouthwestern.edu; 'histonet-requ...@lists.utsouthwestern.edu' Subject: [Histonet] question on H pylori I was just curious what everyone is using for standard of care regarding H = Pylori..is everyone doing IHC or are you doing a Giemsa? Thanks, Lisa Lisa M. Van Valkenberg, B.S., HT- ASCP Histology Manager 2300 Children's Plaza Chicago, IL 60614 773-868-8949 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] question on H pylori
We did diif -quik or Alcian Yellow. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Friday, June 10, 2011 9:10 AM To: Setlak, Lisa; histonet@lists.utsouthwestern.edu; histonet-requ...@lists.utsouthwestern.edu Subject: RE: [Histonet] question on H pylori We do either IHC or a Modified Steiner, depending on the Pathologist's preference. We switched to Newcomer Supply's Steiner-Chapman Modified Silver Stain Kit about a year ago, because it eliminated the use of Uranyl Nitrate. We have been delighted with the consistency of the stain. Sandy Harrison, HTL (ASCP) Anatomical and Surgical Pathology Supervisor, VAMC Minneapolis 612-467-2449 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa Sent: Tuesday, June 07, 2011 1:12 PM To: histonet@lists.utsouthwestern.edu; 'histonet-requ...@lists.utsouthwestern.edu' Subject: [Histonet] question on H pylori I was just curious what everyone is using for standard of care regarding H = Pylori..is everyone doing IHC or are you doing a Giemsa? Thanks, Lisa Lisa M. Van Valkenberg, B.S., HT- ASCP Histology Manager 2300 Children's Plaza Chicago, IL 60614 773-868-8949 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Grimelius protocol for small animals in NY
Diane: Under separate cover I am sending you the Grimelius protocol I used (even when I never worked in New York and even when that is of no relevance!). René J. From: diane.cr...@amcny.org diane.cr...@amcny.org To: histonet@lists.utsouthwestern.edu Sent: Thursday, June 9, 2011 6:43 PM Subject: [Histonet] Grimelius protocol for small animals in NY Hello All, I am fairly new to the world of special stains. My specialty is immunohistochemistry. But from what I understand, our lab and many other animal labs in New York have been unsuccessful in getting the Grimelius stain to work. Does anybody from New York have a working protocol? Thanks! Diane Craft Pathology Department Animal Medical Center 510 East 62nd St New York NY 10065-8314 212-329-8675 (phone) 212-759-5878 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] BRDU
Yes, I am also using pH 6 HIER with 10mM sodium citrate, 0.05% Tween-20. I do 20 minutes at 95 degree C to retrieve BrdU on mouse embryo tissues. I use Abcam ab6326. Their product sheet states that you may used heat for antigen retrieval. The BrdU epitope needs to be revealed by denaturing the double-stranded DNA - and you can do that with an enzyme, acid, or heat - so if you are already doing HIER, the acid/enzyme treatment can be unnecessary (and may even damage the tissue/other antigens you are interested in). Andrea Marion Graduate Student University of Illinois at Chicago I used pH 6 HEIR instead of HCl/enzyme for BrdU - it works very well on mouse and monkey intestine. Did not switch to HCl after that. But now I prefer to use EdU instead of BrdU (Click-iT kit from Invitrogen). It is more expensive, but much more sensitive, fast, easy to use and well compatible with all range of antigens(for multi-color fluorescence). Unfortunately, there are no enzymatic reaction for EdU - only fluorescence. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: AGleiberman @t cbiolabs.com -Original Message- From: histonet-bounces @t lists.utsouthwestern.edu [mailto:histonet-bounces @t lists.utsouthwestern.edu] On Behalf Of C B Sent: Wednesday, June 08, 2011 8:37 PM To: Histonet @t lists.utsouthwestern.edu Subject: [Histonet] BRDU Is anyone using ph6 HEIR instead of HCl/enzyme digestion for BRDU staining? I've read 2 manufacturer datasheets that recommend pH 6 HEIR. I thought the routine pretreatment was HCl/enzyme digestion. We have FFPE rat intestine to stain. Any feeback is appreciated. Cindy Baranowski, HT SJTRI Atlanta, GA ___ Histonet mailing list Histonet @t lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Re:Peloris users
I'm wondering which baskets were used (the ones with the spacers?) and which cassettes (the ones with the solid sides, or slotted?). Does the problem occur without using these recommended items? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Setlak, Lisa Sent: Tuesday, June 07, 2011 1:46 PM To: 'Seguin, Suzanne'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Re:Peloris users Wow, I feel like I'm listening to myself speak. We have the machine for about the same amount of time as yourself and yes we have had tissue contamination. I can't say it's super bad but we've had several cases recently where as before we rarely ever had it. Unfortunately I'm not sure what causes it and I think it got blamed on the grossing person or embedder in our cases. I'm interested to see what everyone has to say about this. Lisa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Seguin, Suzanne Sent: Tuesday, June 07, 2011 12:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:Peloris users Hello, We have been using this tissue processor for 1 year now. During the last 3-6 months, we have experienced an unreasonable number of cases reported with tissue contaminant in paraffin blocks. We use regular tissue cassettes as well as the biopsy and micromesh style. While using this processor I have also witnessed tissue fragment into smaller pieces while processing. Because the Peloris uses a relatively large magnetic stirrer, I'm wondering if this could be a cause for the contaminant. Has anyone else experienced this? Any thoughts, suggestions? Thanks Sue Charge Technologist - Anatomic Pathology Sudbury Regional Hospital 41 Ramsey Lake Road, Sudbury, ON P3E 5J1 (Tel (705) 523-7100 ext. 1209 Fax (705) 675-4776 sseg...@hrsrh.on.ca The information contained in this e-mail and document(s) attached are for the exclusive use of the addressee and may contain confidential, privileged and non-disclosable information. If the recipient of this e-mail is not the addressee, such recipient is strictly prohibited from reading, photocopying, distributing or otherwise using this e-mail or its content in any way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processing schedule for fatty tissues
Hello Everyone: I am looking for a processing schedule for fatty specimens. Could anyone share theirs? Thanks in Advance. :) Sheila Bluewater Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Processing schedule for fatty tissues
Please respond to everyone. I would like to have the information also. Thank you, Carol -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey Sent: Friday, June 10, 2011 4:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing schedule for fatty tissues Hello Everyone: I am looking for a processing schedule for fatty specimens. Could anyone share theirs? Thanks in Advance. :) Sheila Bluewater Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Processing schedule for fatty tissues
The best approach is to increase the fixation time to make sure that the tissues are really fixed. Then cut the dehydration times of the lower alcohols by 20% and add that 20% of saved time as increase in the clearing agent (supposedly xylene or alkane substitute) times. René J. From: Sheila Adey sa...@hotmail.ca To: histonet@lists.utsouthwestern.edu Sent: Friday, June 10, 2011 4:22 PM Subject: [Histonet] Processing schedule for fatty tissues Hello Everyone: I am looking for a processing schedule for fatty specimens. Could anyone share theirs? Thanks in Advance. :) Sheila Bluewater Health ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Grimelius stain
Sara- We stained many tissues for chromogranin and found that the Churukian stain worked a lot better than the Grimelius. It double impregnates the cells with the silver. Directions are in Carson's book, but if you don't have it, I can send them to you. Cheryl Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark Ave. Baton Rouge, LA 70820 (225) 772-2865 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
