RE: [Histonet] Macrophage marker in sheep for IHC
Thank you to everyone who has replied and given suggestions. I now have some ideas to help me start and will update and acknowledge once again once I make some progress. Best regards Vivek -Original Message- From: Jan Shivers [mailto:shive...@umn.edu] Sent: 24 June 2011 14:37 To: Vivek Saroha; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Macrophage marker in sheep for IHC CD68 - clone KP1 has not worked on sheep (also not dogs, cows, horses, and chickens) in my experience. I have found it to stain primates well (and rare cells in pigs and cats). Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Vivek Saroha vivek.sar...@nottingham.ac.uk To: histonet@lists.utsouthwestern.edu Sent: Friday, June 24, 2011 4:05 AM Subject: [Histonet] Macrophage marker in sheep for IHC Hi there, I am very new to basic sciences research and am planning an experiment to quantify and compare adipose tissue macrophage infiltration in sheep model using immunohistochemistry. I am specifically interested in CD11c as a marker of M1 activation of Macrophages. I have been advised that it is not easy and previous effort by my predecessors have not been very successful in this! From the general google, histonet/histosearch and pubmed search. I have made a list of potential candidates: 1.Clones PG M1 and Clone KP1 (Source : Histonet : http://www.histosearch.com/histonet/Nov03A/RE.HistonetSeepmacrophage.html) 2.EMB11, a mouse anti-human CD68 mAb (Dako, Carpenteria, CA) (source: published paper: http://www.ncbi.nlm.nih.gov/pubmed/16460804 ) 1. Another CD68 antibody Source: http://www.ingentaconnect.com/content/ocean/ajra/2011/0025/0002/art00024) 1. Iba1 (ionized calcium binding adaptor molecule 1) is a 17-kDa EF hand protein that is specifically expressed in macrophages/microglia and is upregulated during the activation of these cells.[Wikipedia] (source: I do not remember where I found this one from but Abcam has several antibodies none of which are predicted for sheep) I am writing to ask for suggestions about which one to go for and any other tips about searching for antibodies or performing IHC in paraffin fixed adipose tissue/liver. Any ideas will be gratefully appreciated as I am very new and isolated in this aspect. Best wishes Vivek Dr Vivek Saroha Clinical lecturer in Child health University of Nottingham This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham. This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Tissue Shriveling in Paraffin
You could have got the tissue wet or to hydrated. Sometimes after you face the block if you leave it on the ice to long and let moisture get into the tissue then cut the slide, it looks ok at the time, but once the moisture gets back out of the tissue it will start to look concave. If you are going to be leaving your blocks on the ice for a long time just put a 4X4 or paper towel under the block, this helps. Good luck =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle Aono Sent: Friday, June 24, 2011 8:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Shriveling in Paraffin I was cutting some bone/joint tissue and noticed that the cartilaginous portion was concave/indented, instead of flush with the rest of the block surface. Even as I continued to cut that portion always seemed a little sunken into the block face and all the sections crumbled. I didn't seal the block after I was done and when I came back the next day the entire tissue sample was shriveled and pulling away from the paraffin. I'm new, but in the few bone sections I've done I've never had this happen! Any ideas? ~Shelly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: HistoGel
You can also use agar. It does the same thing and is cheaper =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Milne, Katy Sent: Friday, June 24, 2011 12:27 PM To: 'histonet@lists.utsouthwestern.edu'; 'mjdess...@wvhcs.org' Subject: [Histonet] RE: HistoGel We use histogel a lot in our lab. It's a research lab and we use it for a few purposes - pelleting cultured cells then creating multi-culture TMAs for testing antibodies and also pelleting cells from ascites and pleural effusions. Has also been used to process really small samples that could have been lost in the processor through the cassettes. Works quite well. The researchers just put the samples in histogel and give it to me in formalin then I process it as I would regular tissue. Cuts very well too. Katy Message: 3 Date: Fri, 24 Jun 2011 12:26:46 -0400 From: Dessoye, Michael J mjdess...@wvhcs.org Subject: [Histonet] HistoGel To: histonet@lists.utsouthwestern.edu Message-ID: e2547e1cd0ee324488a2940994571efa0401f...@wvhcs-exchange.wvhcs.com Content-Type: text/plain; charset=iso-8859-1 Hello, Does anyone out there have any experience with HistoGel? It's Richard Allan/Thermo Fisher. They claim that you can embed scant tissues in the gel and then process, embed, and cut as usual. Just wondering how it works in the real world Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] bluing
Just add a little bit more ammonia hydroxide to the water =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, June 24, 2011 2:21 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] bluing Looking to change my bluing step in the HE process to obtain a bluer (less purple) hue to the nuclear detail. What is everyone using in their bluing step?? Thanks for all of your ideas!! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: Re: [Histonet] How many tissues an histo tech is suppose to cutper
Another solution...get out of clinical and go into research =) There are no quotas or slide per second expectations in the research world =) So sorry you are having such a bad time with your job. HT's are not a dime a dozen and usually it is fairly easy to find a better job. Not to mention research pays almost double the clinical world!! Good Luck!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, June 26, 2011 9:43 AM To: histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: Fw: Re: [Histonet] How many tissues an histo tech is suppose to cutper --- Joanne: Read the attachment so you can have an idea about productivity ranges and averages in different tasks in the lab. If you embedded 214 blocks and cut 148 in 5.5 hours, using the averages I provided, embedding should have taken 3.5 hours and cutting 6.2 hours which means you worked 1.76 times FASTER than the expected average. René J. --- On Sat, 6/25/11, Joanne joanne0...@comcast.net wrote: From: Joanne joanne0...@comcast.net Subject: [Histonet] How many tissues an histo tech is suppose to cut per To: histonet@lists.utsouthwestern.edu Date: Saturday, June 25, 2011, 7:07 PM i am quite serious in my presentation and request for advice. i too thought this goal was/is ridiculous to expect/ask for from someone so new and to attain in 6 months or less. last monday i embedded 214 blocks and cut 148 between 5am and 10:30am (we had almost 600 cassettes to share among 3 people) . . . .for someone so very new i thought this pretty good . . . please note: most days aren't as hectic. :) what is an average though for blocks/minute? what is meant by set sum per block? ---keeping in mind i am new to this field. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Full Time HT position in Oklahoma City Ok
POSITION REQUIREMENTS: EDUCATION: High school diploma or GED preferred. CERTIFICATION, LICENSURE, BONDING: Certified as an HT or HLT by the American Society of Clinical Pathologists (ASCP) – or – other nationally recognized certifying agency acceptable to the Laboratory Director – or – experience acceptable to the Laboratory Director. EXPERIENCE: Two years of satisfactory histology experience. SIGN ON BONUS AVAILABLE FOR QUALIFIED CANDIDATES. Outstanding benefits package including generous paid time off. Apply online at www.saintsok.comhttp://www.saintsok.com, Ad# 10762, or contact Anna King at (405) 272-6105 for more information. Thank you! Anna King HR Recruiter St. Anthony Hospital (405) 272-6105 - phone (405) 272-6781 - fax [https://mobile.ssmhc.com/owa/attachment.ashx?id=RgBMsXdcX9YUQLcxZSN9Shu5BwByj4F8AhEOSY2AOnw7DGJIAAUpEpPHAAByj4F8AhEOSY2AOnw7DGJIAAaNIGfxAAAJattcnt=1attid0=EACRQxAfu%2f2MRrpel0TNLrK5] Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] How many tissues...
To add to the recent discussion about how many blocks can be cut per hour, the College of Medical Laboratory Technologists of Ontario published Practice Guidelines for Medical Laboratory Technologists Practising in Histology fairly recently, in 2008, which may be of use in this context. The practice guidelines are intended to support, not replace, the exercise of professional judgment by medical laboratory technologists practising in histology...and are maintained...in consultation with CMLTO members and stakeholders. The usual workplace variables are taken into account in the guideline, but at least there are some daily ranges presented which may help Joanne in her quest for a reasonable goal. Link follows: http://tinyurl.com/histology-guidelines http://tinyurl.com/histology-guidelines Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] One block per minute
It seems like this argument has been going on as long as the histonet = has been here for us. Just because some folks in histoland can cut 60 block= s per minute does not mean that should be the minimum standard, esp for a n= ew tech, come on that is just dangerous to make someone that nervous. = When we purchase a car that states it gets 23 mpg city and 31 mpg on the hi= ghway, those are in perfect conditions when all the stars align, going down= hill in neutral. These so called intutive managers who expect someone= to cut 60 blocks per hour clearly never had a pack of blades that took thr= ee minutes to get a blade to slide out, never had a dull blade, no interrup= tions, no fire alarms,no call for levels, decals, only had perfect processi= ng, perfect soak etc. In 30 plus years I have heard so many people tell me = they can do this, but have never witnessed such an incredible feat, day in = day out. Nick(Rocky) Madary, HT/HTL(ASCP)QIHC Jun 26, 2011 12:01:08 PM, = histonet@lists.utsouthwestern.edu wrote: Send Histonet= mailing list submissions to histonet@lists.utsouthwestern.edu To= subscribe or unsubscribe via the World Wide Web, visit http://lists.uts= outhwestern.edu/mailman/listinfo/histonet or, via email, send a message = with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lis= ts.utsouthwestern.edu When replying, please edit your Subject line s= o it is more specific than Re: Contents of Histonet digest... = BRToday's Topics: 1. for phd offer (mani kandan) 2. Leica Bond f= or IHCs (Nancy Schmitt) 3. Re: Histonet Digest, Vol 91, Issue 34 (Amos B= rooks) 4. How many tissues an histo tech is suppose to cut per (Joanne) BR5. RE: How many tissues an histo tech is suppose to cut per (Rittman,= Barry R) 6. How many tissues an histo tech is suppose to cut per (Joann= e) 7. Re: How many tissues an histo tech is suppose to cut per (histo= t...@imagesbyhopper.com) 8. RE: How many tissues an histo tech is suppos= e to cut per (Thomas Jasper) 9. RE: Leica Bond for IHCs (Houston, Ron= ald) 10. RE: How many tissues an histo tech is suppose to cut per (Th= omas Jasper) 11. Re: How many tissues an histo tech is suppose to cut pe= r (Victoria Baker) 12. Re: AL state meeting? (David Kemler) 13. RE= : How many tissues an histo tech is suppose to cut per (WILLIAM DESALVO) 14. Re: How many tissues an histo tech is suppose to cut per (histot= e...@imagesbyhopper.com) 15. RE: Leica Bond for IHCs (Horn, Hazel V) 1= 6. Re: How many tissues an histo tech is suppose to cut per (Rene J Bues= a) 17. Fw: Re: [Histonet] How many tissues an histo tech is suppose t= o cut per (Rene J Buesa) 18. Sakura auto TEC and the Leica cassette prin= ter (Denise Mattingly) - - Message: 1 Date: Sat, 25 Jun 2011 2= 3:06:06 +0530 (IST) From: mani kandan Subject= : [Histonet] for phd offer To: histonet@lists.utsouthwestern.edu Mess= age-ID: 1309023366.93666.yahoomailclas...@web94702.mail.in2.yahoo.co= m Content-Type: text/plain; charset=iso-8859-1 Hai hist= onetters, n= bsp;i am a master of science graduate working in stemcell research,looking for a phd offer or RA offer, currently= i am working on cell derived from bone enosteal and central region cells. = i am looking for research offer related to this field. i am looking for fav= ourable reply. thank u. M.Manikandan, Researcher, Stemcell uni= t, King Saud university, Riyadh,KSA +966552012697 -= - Message: 2 Date: Sat, 25 Jun 2011 17:49:21 = + From: Nancy Schmitt Subject: [Histon= et] Leica Bond for IHCs To: histonet@lists.utsouthwestern.edu Message-ID: 906B4DA90ED1DB4DB6C7E9= 4d7cee6c36790...@peitha.wad.pa-ucl.com Content-Type: text/plain; cha= rset=us-ascii We are very happy with our BOND and technical supp= ort has been great for any and all questions/issues. Nancy Schmitt H= T,MLT(ASCP) Dubuque, IA -- Message= : 16 Date: Sat, 25 Jun 2011 12:00:44 -0400 From: Sheila Adey Subject: [Histonet] Leica Bond for IHCs To: Message-ID: Content-Type: text/plain; charset=iso-8859-1 Hello= netters: Looking for opinions on the Leica Bond immuno stainer please. Thanks. Sheila NOTICE: This email may contain legal= ly privileged information. The information is for the use of only the in= tended recipient(s) even if
RE: [Histonet] How many tissues an histo tech is suppose to cut per
The only time I have seen techs cut that fast was when there was a team - one cuts, the other picks up the section and labels the slides. These were Aussies and Kiwis I worked with overseas and that is how they work in some places. BTW, they also stand at the bench and put the microtome sideways in order to work faster (pull the ribbon off, throw it on the waterbath and go on to the next block. Otherwise, forget it, it is not possible and still keep quality and sanity intact. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Interesting article on a Healthcare Camp to educate children on the healthcare professions.
Hi Histonetters, I found this article on a healthcare camp to educate kids on the healthcare professions: http://billingsgazette.com/news/state-and-regional/wyoming/article_06923 0e4-c77e-5b4d-95e0-bd9741f0595f.html Have a great day! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histo tech is suppose to cut per
At one place I worked we teamed like this and it was so efficient! It also helped with error reduction. The main problem I see for Joanne is the getting up to answer the phone, fielding questions regarding send-out cases, and other slight cutting interruptions. No one can predict how long these interruptions would take. Joanne, I think I would ask for uninterrupted time in the beginning to hone your cutting skills. As your confidence builds, so will your speed. And so will the time to refocus after an interruption. I rarely cut these days and when I do I am slower than slow. Best wishes, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, June 27, 2011 11:15 To: 'Joanne'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per The only time I have seen techs cut that fast was when there was a team - one cuts, the other picks up the section and labels the slides. These were Aussies and Kiwis I worked with overseas and that is how they work in some places. BTW, they also stand at the bench and put the microtome sideways in order to work faster (pull the ribbon off, throw it on the waterbath and go on to the next block. Otherwise, forget it, it is not possible and still keep quality and sanity intact. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histogel Protocol FROZEN Histonet Digest, Vol 91, Issue 34
Katy (or anyone else who has used Histogel for FROZEN samples), I am using Histogel mixed with OCT to create a frozen cell pellet. I was successful with doing a single pellet but now I am being asked to do multiple and have found hit some challenges. I have never done a TMA but that sounds like a great option. We are a research lab but using the Cell Pellet for a normalizing control of IF intensity (for computational analysis normalization for lung tissue) and one of the largest challenges is retaining cell density while still trying to cryoprotect and freeze well with the OCT surrounding it. Has anyone worked out a method for frozen cell pellets to embedded with multiple cell lines? Most of what I have found has been from FFPE, any suggestions would be appreciated. Thank you! Kathy Kathy M. Bonness, PhD. 251-533-2661 kathy.bonn...@utsouthwestern.edu http://www.linkedin.com/pub/kathy-bonness/6/1a1/931 UTSW Dallas, TX Green Center for Computational Systems Biology Department of Pharmacology Altschuler/Wu Lab (ND9.214) From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Saturday, June 25, 2011 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 91, Issue 34 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: HistoGel (Milne, Katy) 2. Histogel Problem (Amos Brooks) 3. RE: Histogel Problem (Delossantos_Roseann) 4. bluing (Webb, Dorothy L) 5. Re: bluing (Rene J Buesa) 6. RE: bluing (Harrison, Sandra C.) 7. blades (Webb, Dorothy L) 8. Re: blades (Sean McBride) 9. Re: blades (Esther C Peters) 10. Re: blades (Victoria Baker) 11. Re: blades (Jennifer MacDonald) 12. HT Position - Irvine, CA (Eric Velazquez) 13. Re: HT Position - Irvine, CA (Lee Peggy Wenk) 14. Contents of Histonet digest (Aurea Marquez) 15. Re: bluing (Lee Peggy Wenk) 16. Leica Bond for IHCs (Sheila Adey) 17. RE: Bluing (gayle callis) -- Message: 1 Date: Fri, 24 Jun 2011 10:27:09 -0700 From: Milne, Katy kmi...@bccancer.bc.ca Subject: [Histonet] RE: HistoGel To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu,'mjdess...@wvhcs.org' mjdess...@wvhcs.org Message-ID: 3feff18ff4e1914a9ab7d8498591be8610cf058...@vexccr02.phsabc.ehcnet.ca Content-Type: text/plain; charset=us-ascii We use histogel a lot in our lab. It's a research lab and we use it for a few purposes - pelleting cultured cells then creating multi-culture TMAs for testing antibodies and also pelleting cells from ascites and pleural effusions. Has also been used to process really small samples that could have been lost in the processor through the cassettes. Works quite well. The researchers just put the samples in histogel and give it to me in formalin then I process it as I would regular tissue. Cuts very well too. Katy Message: 3 Date: Fri, 24 Jun 2011 12:26:46 -0400 From: Dessoye, Michael J mjdess...@wvhcs.org Subject: [Histonet] HistoGel To: histonet@lists.utsouthwestern.edu Message-ID: e2547e1cd0ee324488a2940994571efa0401f...@wvhcs-exchange.wvhcs.com Content-Type: text/plain; charset=iso-8859-1 Hello, Does anyone out there have any experience with HistoGel? It's Richard Allan/Thermo Fisher. They claim that you can embed scant tissues in the gel and then process, embed, and cut as usual. Just wondering how it works in the real world Michael J. Dessoye, M.S. | Histology Supervisor | Wyoming Valley Health Care System | mjdess...@wvhcs.org mailto:mjdess...@wvhcs.org | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1485 | Fax: 570-552-1526 -- Message: 2 Date: Fri, 24 Jun 2011 13:29:09 -0400 From: Amos Brooks amosbro...@gmail.com Subject: [Histonet] Histogel Problem To: histonet@lists.utsouthwestern.edu Message-ID: BANLkTinbTG=qcs3peuf8zfa3bz2wqrt...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi, I have a problem with some blocks that were prepared with Histogel. I was hoping someone else might have had a similar problem and figured it out. I took a photo of the blocks that were mads and put them in a Picassa album here:
RE: [Histonet] How many tissues an histo tech is suppose to cut per
Unless you are in some remote area that there aren't any other facilities around, I would look for a new job! I don't think your age should have any bearing on finding one. If you were close to me I'd hire you. Working under that kind of condition is unacceptable in my opinion. It promotes errors and that isn't what we are all about. Those blocks are our patients. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histo tech is suppose to cut per
I agree with Tim. I have been in the field for over 20 years, and this is an unrealistic goal even for a veteran. Your manager obviously has no experience or understanding of your job in order to set that type of goal for you. It would be possible if the blocks are already chilled, the slides pre-labeled, and you have a helper to load the slides in the racks and onto the stainer. Even then there will be some sacrifice of quality for quantity. Just my two cents. Lori -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Monday, June 27, 2011 8:15 AM To: 'Joanne'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per The only time I have seen techs cut that fast was when there was a team - one cuts, the other picks up the section and labels the slides. These were Aussies and Kiwis I worked with overseas and that is how they work in some places. BTW, they also stand at the bench and put the microtome sideways in order to work faster (pull the ribbon off, throw it on the waterbath and go on to the next block. Otherwise, forget it, it is not possible and still keep quality and sanity intact. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histogel
Histogel is indeed expensive, and often not permitted by Management for that reason. Preparing your own agar is a bit tricky - you really need a hot plate and a magnetic stirrer, unlikely items in a histology lab. (2% or 3% dry agar, in water.) Back before hospital microbiology turned into the black box it is today, you used to be able to walk across the hall and pick up a tube of trypticase soy agar (TSA - I think it was 3% agar with some stuff in it to make bugs grow) and use that. One way to use agar is for the pathologist to pour out some melted agar on a glass slide or metal ruler, and embed small specimens in it so they stay oriented - works great for temporal artery biopsies and vasectomy specimens - time consuming. A blood bank heating block is useful for keeping tubes of agar melted at the gross desk. Whether you use Histogel or some other agar, it's very important not to commit any valuable specimens to it until you're sure it works in your system. I like to carve out some pseudo-biopsies from a normal mucosa in a colon resection specimen and run them. - I've seen some disasters when this precaution wasn't taken. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histo tech is suppose to cut per
I second this motion!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, June 27, 2011 12:10 PM To: 'Joanne'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per Unless you are in some remote area that there aren't any other facilities around, I would look for a new job! I don't think your age should have any bearing on finding one. If you were close to me I'd hire you. Working under that kind of condition is unacceptable in my opinion. It promotes errors and that isn't what we are all about. Those blocks are our patients. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: Re: [Histonet] How many tissues an histo tech is suppose to cutper
As a manager I would never expect that kind of turn around. I think it's impossible to achieve. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's WaySlot 820 Little Rock, AR 72202 phone 501.364.4240 fax501.364.3155 visit us on the web at:www.archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: Monday, June 27, 2011 9:38 AM To: rjbu...@yahoo.com; histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: RE: Re: [Histonet] How many tissues an histo tech is suppose to cutper Another solution...get out of clinical and go into research =) There are no quotas or slide per second expectations in the research world =) So sorry you are having such a bad time with your job. HT's are not a dime a dozen and usually it is fairly easy to find a better job. Not to mention research pays almost double the clinical world!! Good Luck!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, June 26, 2011 9:43 AM To: histonet-requ...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: Fw: Re: [Histonet] How many tissues an histo tech is suppose to cutper --- Joanne: Read the attachment so you can have an idea about productivity ranges and averages in different tasks in the lab. If you embedded 214 blocks and cut 148 in 5.5 hours, using the averages I provided, embedding should have taken 3.5 hours and cutting 6.2 hours which means you worked 1.76 times FASTER than the expected average. René J. --- On Sat, 6/25/11, Joanne joanne0...@comcast.net wrote: From: Joanne joanne0...@comcast.net Subject: [Histonet] How many tissues an histo tech is suppose to cut per To: histonet@lists.utsouthwestern.edu Date: Saturday, June 25, 2011, 7:07 PM i am quite serious in my presentation and request for advice. i too thought this goal was/is ridiculous to expect/ask for from someone so new and to attain in 6 months or less. last monday i embedded 214 blocks and cut 148 between 5am and 10:30am (we had almost 600 cassettes to share among 3 people) . . . .for someone so very new i thought this pretty good . . . please note: most days aren't as hectic. :) what is an average though for blocks/minute? what is meant by set sum per block? ---keeping in mind i am new to this field. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Oncotype
I was wandering how many of you out there are using oncotype dx. for some of your breast patients? And what is your handling process for this test? Thanks in advance, Amy Amy Self Georgetown Hospital System NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Oncotype
We do this testing for oncology. They pay for the shipping via fedex. The oncologist fills out the requsition and the lab pulls the block , packages it and ships it. Andrea On 2011-06-27, at 2:10 PM, Amy Self as...@georgetownhospitalsystem.org wrote: I was wandering how many of you out there are using oncotype dx. for some of your breast patients? And what is your handling process for this test? Thanks in advance, Amy Amy Self Georgetown Hospital System NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Oncotype
When I was working in the lab, we sent specimens for Oncotype. They perform. 21 gene assay to predict the chance of recurrence of the cancer within 10 years. You can either send the tissue block or slides (depending on your send out policy). The testing can have an impact on the patient's tratment. Sherene - Original Message - From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Mon Jun 27 14:10:59 2011 Subject: [Histonet] Oncotype I was wandering how many of you out there are using oncotype dx. for some of your breast patients? And what is your handling process for this test? Thanks in advance, Amy Amy Self Georgetown Hospital System NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How many tissues an histo tech is suppose to cut per
I have known one or two people who can attain this rate. However with them, it is not consistent, especially if there are any interruptions. I would take a couple of articles, especially some of Rene Buesa's authoritative articles to your management and encourage them to set realistic expectations. If your manager has any experience in histology, ask them to show you how to do it, (put them on the spot to try to meet their own goals). Good luck. On Mon, Jun 27, 2011 at 10:51 AM, sgoe...@mirnarx.com wrote: I second this motion!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Monday, June 27, 2011 12:10 PM To: 'Joanne'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How many tissues an histo tech is suppose to cut per Unless you are in some remote area that there aren't any other facilities around, I would look for a new job! I don't think your age should have any bearing on finding one. If you were close to me I'd hire you. Working under that kind of condition is unacceptable in my opinion. It promotes errors and that isn't what we are all about. Those blocks are our patients. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histo tech is suppose to cut per
The proper way to do this would be to time each tech in your lab as to how many blocks they can cut in one hour. Take everyones number of blocks and do an average for your lab. The average for my lab is 2.15 minutes per block. I compiled these numbers in an effort to determine what our labor costs are, not to set an expectation for productivity. I have some people who cut really fast and some who cut slow. I am curious as to how your management came up with these numbers and are any of them histotechs?? Christie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Sent: Saturday, June 25, 2011 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How many tissues an histo tech is suppose to cut per i've only been working 2 months. although older, i am new as a histotech (graduated in may 2010, found a job in april 2011). seems management is setting a goal of a block per minute as far as cutting goes for me. i have until october to attain this goal. this minute for cutting is to include facing, writing out slides, cutting, and putting tray into symphony stainer (not to mention getting up to answer the phone, fielding questions regarding send-out cases, and other slight cutting interruptions). this seems an extreme, possibly unattainable goal. i'm up for a challenge at age 53, but any advice would be SWONDERFUL :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Austin PRN
Hello all =) Since I just recently got married and still own a house we do not live in, I am finding myself in the pickle of 2 houses and not enough income...Does anyone know of any kind of part time histology jobs in Austin that I could go to either early until around 7am or late after 5pm? Or if not histology specific, I could log specimens for intake or something...I just can't bring myself to ask the question...would you like fries with that? with a college education and 10 years of histology experience...I'm doing ok, I just want my fluff back =) Thanks ya'll!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Invitation to connect on LinkedIn
LinkedIn Thomas Huynh requested to add you as a connection on LinkedIn: -- David, I'd like to add you to my professional network on LinkedIn. - Thomas Accept invitation from Thomas Huynh http://www.linkedin.com/e/yvpgd1-gpfyh2hc-1e/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I124609207_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYTc38Vc3oQcz59bQRLsDpOqkRVbP8QcjcOe3oRcPcLrCBxbOYWrSlI/EML_comm_afe/ View invitation from Thomas Huynh http://www.linkedin.com/e/yvpgd1-gpfyh2hc-1e/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I124609207_13/3cNnPsMczAMdzgOckALqnpPbOYWrSlI/svi/ -- DID YOU KNOW LinkedIn can help you find the right service providers using recommendations from your trusted network? Using LinkedIn Services, you can take the risky guesswork out of selecting service providers by reading the recommendations of credible, trustworthy members of your network. http://www.linkedin.com/e/yvpgd1-gpfyh2hc-1e/svp/inv-25/ -- (c) 2011, LinkedIn Corporation ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Flk2 / Flt3 / CD135 on mouse bone
Hi all, One of my fellow graduate students is trying to perform immunofluorescence on PFA fixed, EDTA decalcified mouse bone for Flk2 (aka Flt3 or CD135). So far, he hasn't had any luck on either paraffin embedded or frozen sections using HIER. Has anyone done this successfully? I know the ideal way to perform IF on bone is using a tape-transfer system, but, alas, we don't quite have that working yet. Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Flk2 / Flt3 / CD135 on mouse bone
Adam/his fellow grad student, I could be somewhat off not being up to date with current literature. Sorry. But used to look for Flk2/Flt3/CD135 in murine bone marrow and not the bone itself. In immature hematopoeitic progenitor cells. Along with looking in thymus, etc. But for something like femurs, I removed the bone marrow, and there are some slick ways to do it without disrupting the core architecture too much. Could take it out and obviously flow (cytometry) the cells. But could also get out a fairly intact core and freeze it, or paraffin process/section it or glycol methacrylate section it and worry very little about the minute amount of trabecular bone in murine bone marrow. Depending on age, etc of course. So unless for the specific needs of the project call for looking actually for staining within the cortical bone, I'd take that problem out of the picture by getting the bone marrow out and then not having to worry about decalcification and tape transfer and things like that. Makes IHC staining a lot easier in my experience but perhaps there are those out there who have worked out whole, intact mouse bone CD135 staining. Ray Ray Koelling PhenoPath Labs Seattle WA - Original Message - From: Adam . anonwu...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Monday, June 27, 2011 5:49:23 PM Subject: [Histonet] Flk2 / Flt3 / CD135 on mouse bone Hi all, One of my fellow graduate students is trying to perform immunofluorescence on PFA fixed, EDTA decalcified mouse bone for Flk2 (aka Flt3 or CD135). So far, he hasn't had any luck on either paraffin embedded or frozen sections using HIER. Has anyone done this successfully? I know the ideal way to perform IF on bone is using a tape-transfer system, but, alas, we don't quite have that working yet. Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Flk2 / Flt3 / CD135 on mouse bone
Hi Ray, That is a great idea. We are indeed looking for Flk2 expression on hematopoietic progenitors cells, but alas, we are looking at these cells in relationship to bone elements such as osteoblasts, so we need to do it on intact bone. Adam On Mon, Jun 27, 2011 at 8:43 PM, koelli...@comcast.net wrote: Adam/his fellow grad student, I could be somewhat off not being up to date with current literature. Sorry. But used to look for Flk2/Flt3/CD135 in murine bone marrow and not the bone itself. In immature hematopoeitic progenitor cells. Along with looking in thymus, etc. But for something like femurs, I *removed* the bone marrow, and there are some slick ways to do it without disrupting the core architecture too much. Could take it out and obviously flow (cytometry) the cells. But could also get out a fairly intact core and freeze it, or paraffin process/section it or glycol methacrylate section it and worry very little about the minute amount of trabecular bone in murine bone marrow. Depending on age, etc of course. So unless for the specific needs of the project call for looking actually for staining within the cortical bone, I'd take that problem out of the picture by getting the bone marrow out and then not having to worry about decalcification and tape transfer and things like that. Makes IHC staining a lot easier in my experience but perhaps there are those out there who have worked out whole, intact mouse bone CD135 staining. Ray Ray Koelling PhenoPath Labs Seattle WA -- *From: *Adam . anonwu...@gmail.com *To: *histonet@lists.utsouthwestern.edu *Sent: *Monday, June 27, 2011 5:49:23 PM *Subject: *[Histonet] Flk2 / Flt3 / CD135 on mouse bone Hi all, One of my fellow graduate students is trying to perform immunofluorescence on PFA fixed, EDTA decalcified mouse bone for Flk2 (aka Flt3 or CD135). So far, he hasn't had any luck on either paraffin embedded or frozen sections using HIER. Has anyone done this successfully? I know the ideal way to perform IF on bone is using a tape-transfer system, but, alas, we don't quite have that working yet. Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
