Re: AW: [Histonet] Quick Drying Mounting Media
Have you tried drying them under a blow dryer? That's how we used to dry our slides before we sent them out. It was a commercially available slide dryer. Michelle Sent from my iPhone On Jul 14, 2011, at 3:57 AM, Gudrun Lang gu.l...@gmx.at wrote: I think it's more depending on the organic solvent used before coverslipping. We use butyl acetate in the last trogh in the stainer and in the coverslipper. This reagent dries/ evaporates very fast. So after 15 min there is hardly a smell of it and we deliver our slides immediately. We use Pertex as coverslipping medium. Gudrun Histolab, Akh Linz, Austria -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Katelin Lester Gesendet: Mittwoch, 13. Juli 2011 19:42 An: Histonet Betreff: [Histonet] Quick Drying Mounting Media Hi histonet, We have a brand new Leica CV5030 Coverslipper and I am looking for the quickest drying media available. We are currently using Micromount by Surgipath/Leica and it is not drying fast enough. Any suggestions are appreciated as we have to package our slides for shipping and the media is either sticking to the bubble wrap or creating circles on the slides from the pressure of the bubble wrap. -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] And now, IHC playing cards!
Do you have to buy anything to get them? Or do you just go to their website? A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Wed, Jul 13, 2011 at 3:18 PM, mtitf...@aol.com wrote: Those of you that like freebies (like Dako mousepads, Thermo pens and Sigma coffee mugs) will be amused to learn that Cell Marque are giving away packs of playing cards. Each card shows a section stained with a different antobody (one of theirs of course, together with the catalogue number). No, I don't have shares in Cell Marque, but I am continually amused by the different methods companies in all walks of life use to advertise their products. Some are, well, just original! Michael Titford Pathology USA Mobile AL USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] And now, IHC playing cards!
Hello again! I went to their site and can't find anything...hopefully I didn't miss it!! Emily A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Wed, Jul 13, 2011 at 3:18 PM, mtitf...@aol.com wrote: Those of you that like freebies (like Dako mousepads, Thermo pens and Sigma coffee mugs) will be amused to learn that Cell Marque are giving away packs of playing cards. Each card shows a section stained with a different antobody (one of theirs of course, together with the catalogue number). No, I don't have shares in Cell Marque, but I am continually amused by the different methods companies in all walks of life use to advertise their products. Some are, well, just original! Michael Titford Pathology USA Mobile AL USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Antigen retrieval survey
Citrate buffer René J. --- On Wed, 7/13/11, Andrea T. Hooper andreahoo...@rocketmail.com wrote: From: Andrea T. Hooper andreahoo...@rocketmail.com Subject: [Histonet] Antigen retrieval survey To: Histonet histonet@lists.utsouthwestern.edu Date: Wednesday, July 13, 2011, 11:10 PM Hi All, I am doing a survey and will be happy to compile results and share if folks will respond! What is your favorite antigen retrieval method and/or panel? Buffer Source/composition Temperature Device Thanks, Andrea ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 92, Issue 18
On all stomach biopsies. Tim - Original Message - From: histonet-requ...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Wednesday, July 13, 2011 10:45 PM Subject: Histonet Digest, Vol 92, Issue 18 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Quick Drying Mounting Media (Katelin Lester) 2. Re: Quick Drying Mounting Media (Katelin Lester) 3. IHC for H. pylori (Joanne Clark) 4. Re: IHC for H. pylori (Patrick Laurie) 5. QC vs QA (H R) 6. RE: QC vs QA (Amber McKenzie) 7. Re: IHC for H. pylori (Richard Cartun) 8. Re: IHC for H. pylori (Mark Tarango) 9. RELIA Hot Management Job Alert Anatomic Pathology Lab Manager for top Lab in Austin, TX (Pam Barker) 10. Nbldsu (Paul Scholder) 11. And now, IHC playing cards! (mtitf...@aol.com) 12. Re: (Denise Piontek) 13. Question - Anatomic Pathology Manager (Richard Cartun) 14. Tzank Smears (Amy Self) 15. SlideMate Procedure (Laurie Colbert) 16. RE: IHC for H. pylori (Fawn Bomar) 17. Reference labs doing Fast Myosin IHC stain (Sebree Linda A) 18. Re: QC vs QA (SHANE NELSON) 19. Bone Marrow Biopsies (Heather Cooper) 20. out of office (marilyn.a.we...@kp.org) 21. Antigen retrieval survey (Andrea T. Hooper) 22. Re: confocal questions: mixing fluorescent dyes with proteins (Andrea T. Hooper) 23. Re: In search of a cryostat... (Andrea T. Hooper) 24. Anti-RFP (Andrea T. Hooper) -- Message: 1 Date: Wed, 13 Jul 2011 10:42:25 -0700 From: Katelin Lester katelin09...@gmail.com Subject: [Histonet] Quick Drying Mounting Media To: Histonet histonet@lists.utsouthwestern.edu Message-ID: CAOEzYbS9j+_CT5UWZ5Hh9CiXT8wEXCyqLDuiNCrYij2=wat...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi histonet, We have a brand new Leica CV5030 Coverslipper and I am looking for the quickest drying media available. We are currently using Micromount by Surgipath/Leica and it is not drying fast enough. Any suggestions are appreciated as we have to package our slides for shipping and the media is either sticking to the bubble wrap or creating circles on the slides from the pressure of the bubble wrap. -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 -- Message: 2 Date: Wed, 13 Jul 2011 11:14:01 -0700 From: Katelin Lester katelin09...@gmail.com Subject: Re: [Histonet] Quick Drying Mounting Media To: Marcia Funk fu...@mercyhealth.com, Histonet histonet@lists.utsouthwestern.edu Message-ID: caoezybtbrtaisapoc9mvl7mz7ruawq2ohqmcbszmvoeytoo...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 We are shipping them out same day. At this point, if I can not find a quicker drying media I will have to delay the shipments by one day. What temperature do you dry them at? On Wed, Jul 13, 2011 at 10:50 AM, Marcia Funk fu...@mercyhealth.com wrote: Katelin- do you ship the dame day ? We dry our slides in a oven daily and file ship the next day . seems to work Marcia Marcia Funk Histology Laboratory Mercy Medical Center North Iowa Mason City, IA, 50401 641-428-7907 Katelin Lester katelin09...@gmail.com 7/13/2011 12:42 PM Hi histonet, We have a brand new Leica CV5030 Coverslipper and I am looking for the quickest drying media available. We are currently using Micromount by Surgipath/Leica and it is not drying fast enough. Any suggestions are appreciated as we have to package our slides for shipping and the media is either sticking to the bubble wrap or creating circles on the slides from the pressure of the bubble wrap. -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 -- Message: 3 Date: Wed, 13 Jul 2011 18:22:17 + From: Joanne Clark jcl...@pcnm.com Subject: [Histonet] IHC for H. pylori To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 0494a7d4e8cc254ea2fb81464982e3786e6...@s10maild001n1.sh10.lan Content-Type: text/plain; charset=us-ascii Hi All, was wondering how everyone does their IHC for H. pylori. Do you automatically run it on all biopsies of the
RE: [Histonet] Antigen retrieval survey
I hate to be pessimistic, but different clones of monoclonal antibodies can have different optimum retrieval requirements, not to mention tissue type, source species, etc. What I do believe however, is that enzymatic retrieval is under-sold - no equipment purchase is necessary so you will not see companies advocating their use. Nearly half of the antibodies we use are optimally retrieved with enzymes (pronase, trypsin, pepsin) rather than heat retrieval. Tresa Goins Veterinary Diagnostic Lab South 19th and Lincoln Bozeman, MT 59718 406-994-6353 - phone 406-994-6344 - fax Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea T. Hooper Sent: Wednesday, July 13, 2011 9:10 PM To: Histonet Subject: [Histonet] Antigen retrieval survey Hi All, I am doing a survey and will be happy to compile results and share if folks will respond! What is your favorite antigen retrieval method and/or panel? Buffer Source/composition Temperature Device Thanks, Andrea ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] tissue processing problem
We have several tissue blocks with cells that have shrunken. The pathologist called them shrink o cytes. The tissue affected is mostly small gastric polyps. This sounds like a dehydration issue. Any suggestions would be greatly appreciated. Thank you, Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Position Alert - Field Histology Southern CA
Good Morning Everyone, New Position Alert!! One of our retained Cancer Diagnostics clients has opened up an Field Technical Support Opportunity based in Southern CA. This position is perfect for a Histotech looking to get out of the lab and into the field. Field Support Specialist - IHC The primary responsibility of this role will be to provide in-field technical applications support for current and next generation range of products. The FSS will install/set-up instrumentation in customer laboratories, perform demonstrations and maintain demonstration equipment in a clean and operational manner. The FSS will also train customers on the use of instrumentation. Conducting in-field post purchase applications support and performing instrument validations will be a key responsibility of this position. Position offers: Competitive Base Salary + Bonus/Commission + $700/Month for a Car Allowance and Gas Card, Cell Phone, Laptop, 401k, Full Benefits Open due to Promotion!! Please send your resumes to m...@personifysearch.com, or call me at 800.875.6188 ext. 103 Also, if you know of anyone who may be interested please do not hesitate to forward this e-mail, and have them reach out to me. Have a great day! Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Antigen Retrieval
Citrate buffer is my first port of call. Jim Reilly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] tissue processing problem
You are right, it is most likely a dehydration issue. Try to start with 70EthOL and increase gradually until 100EthOL René J. --- On Thu, 7/14/11, Carol Bryant cb...@lexclin.com wrote: From: Carol Bryant cb...@lexclin.com Subject: [Histonet] tissue processing problem To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Thursday, July 14, 2011, 10:58 AM We have several tissue blocks with cells that have shrunken. The pathologist called them shrink o cytes. The tissue affected is mostly small gastric polyps. This sounds like a dehydration issue. Any suggestions would be greatly appreciated. Thank you, Carol Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Antigen retrieval survey
96 degree water bath for half an hour in either citrate buffer (pH 6) or Tris-HCl (pH 9), depending on which gives better retrieval for that antibody. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrea T. Hooper Sent: Wednesday, July 13, 2011 11:10 PM To: Histonet Subject: [Histonet] Antigen retrieval survey Hi All, I am doing a survey and will be happy to compile results and share if folks will respond! What is your favorite antigen retrieval method and/or panel? Buffer Source/composition Temperature Device Thanks, Andrea ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Autoclave water
Hello all, We are having an issue with water (Millipore filtered and tap is the same problem). We put the water into glass pyrex jars (the kind with the orange lid) and autoclave. After they are done there are tiny little naked eye visible particles of stuff floating in the water. It shimmers and almost looks like small shards of glass? We bought new jars and it seemed to be fine for awhile, but the particles are back...any ideas?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: IHC for H. pylori
The three pathology services I've worked in in the last few yers that did immunohistochemical staining for Helicobacter pylori did it on all gastric biopsy specimens. Usually R/O H.p. is on the requisition. I prefer a blue stain (Giemsa or Diff-Quik II or equivalent) if I have one slide a day to read, IHC if I have ten. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Autoclave water
Same with deionized, filtered water? /daniel On Thu, Jul 14, 2011 at 5:51 PM, sdys...@mirnarx.com wrote: Hello all, We are having an issue with water (Millipore filtered and tap is the same problem). We put the water into glass pyrex jars (the kind with the orange lid) and autoclave. After they are done there are tiny little naked eye visible particles of stuff floating in the water. It shimmers and almost looks like small shards of glass? We bought new jars and it seemed to be fine for awhile, but the particles are back...any ideas?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Autoclave water
Have you tried glass lids? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Sjolander Sent: Thursday, July 14, 2011 12:51 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Autoclave water Same with deionized, filtered water? /daniel On Thu, Jul 14, 2011 at 5:51 PM, sdys...@mirnarx.com wrote: Hello all, We are having an issue with water (Millipore filtered and tap is the same problem). We put the water into glass pyrex jars (the kind with the orange lid) and autoclave. After they are done there are tiny little naked eye visible particles of stuff floating in the water. It shimmers and almost looks like small shards of glass? We bought new jars and it seemed to be fine for awhile, but the particles are back...any ideas?? Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] And now, IHC playing cards!
I emailed my rep and asked her to send me some. I'm studying for the QIHC and told her it would be a good study tool. Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Thursday, July 14, 2011 8:46 AM To: mtitf...@aol.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] And now, IHC playing cards! Do you have to buy anything to get them? Or do you just go to their website? A great book should leave you with many experiences, and slightly exhausted. You should live several lives while reading it. -William Styron On Wed, Jul 13, 2011 at 3:18 PM, mtitf...@aol.com wrote: Those of you that like freebies (like Dako mousepads, Thermo pens and Sigma coffee mugs) will be amused to learn that Cell Marque are giving away packs of playing cards. Each card shows a section stained with a different antobody (one of theirs of course, together with the catalogue number). No, I don't have shares in Cell Marque, but I am continually amused by the different methods companies in all walks of life use to advertise their products. Some are, well, just original! Michael Titford Pathology USA Mobile AL USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet /preThe contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.brOnly the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone./pre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lab humidity range
We're prepping for a Big Inspection (I could tell you who but then I'd have to enter the Witness Protection Program). The Inspecting Organization will want me to cite a range for HUMIDITY in the lab. Because this not only hinges on our weather (we're in Monsoon Season right now and the lab humidity is 47% as I write but is normally between 17-20%), but also MUST be based on any of the histology equipment that may be affected by humidity in any way. Realizing that we all work in different environments and usually have little or no control over the HVAC system, what did you decide upon as your HUMIDITY RANGE for the SOP under which you operate? If there are no specific equipment requirements, I will be able to give a range of 0-100% without the necessity for a Corrective Action Report. If I am too specific and give too narrow a range, I may end up filling out a Corrective Action Report for days when I'm out of range. Please give me as much detail as you are able, without being required to join me in hiding. Your input will be rewarded (don't ask). Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] P16
What are folks using for P16? Thanks for your help- Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TTF-1
Is anyone using the SPT24 clone for TTF-1 using the Ultraview platform on the Benchmark XT? Our pathologists have read this clone is supposed to be more sensitive than Ventana's 8G7G3/1 clone Thank you Anna anna.in...@stmarygj.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] TTF-1
My lab uses the SPT24 clone for TTF-1. Let me know if you'd like any protocol info. Mark On Thu, Jul 14, 2011 at 11:47 AM, Inman, Anna anna.in...@stmarygj.orgwrote: Is anyone using the SPT24 clone for TTF-1 using the Ultraview platform on the Benchmark XT? Our pathologists have read this clone is supposed to be more sensitive than Ventana's 8G7G3/1 clone Thank you Anna anna.in...@stmarygj.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Lab humidity range
We chose 10 - 90% as our range after reading what few instrument manuals even addressed the issue. I can only wish we could get to 17% humidity here in NC. Martha Ward, MT (ASCP) QIHC Assistant Manager Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, July 14, 2011 2:23 PM To: Histonet@lists.utsouthwestern.edu Cc: Hanosh, Timothy J.; Jillson, Gregory Subject: [Histonet] Lab humidity range We're prepping for a Big Inspection (I could tell you who but then I'd have to enter the Witness Protection Program). The Inspecting Organization will want me to cite a range for HUMIDITY in the lab. Because this not only hinges on our weather (we're in Monsoon Season right now and the lab humidity is 47% as I write but is normally between 17-20%), but also MUST be based on any of the histology equipment that may be affected by humidity in any way. Realizing that we all work in different environments and usually have little or no control over the HVAC system, what did you decide upon as your HUMIDITY RANGE for the SOP under which you operate? If there are no specific equipment requirements, I will be able to give a range of 0-100% without the necessity for a Corrective Action Report. If I am too specific and give too narrow a range, I may end up filling out a Corrective Action Report for days when I'm out of range. Please give me as much detail as you are able, without being required to join me in hiding. Your input will be rewarded (don't ask). Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Humidididity Ranges
I love your replies because it gives me quite a range in order to avoid Incarceration, but I need to know THE CRITERIA (specific equipment operating parameters, for example?) you used to come to that range. I'm walkin' the plank here and I'm getting close to the end... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histobath revised
We continue to use the Histobath, but the istrument is no longer being produced. I suggest you search the instruent resellers for a unit. We have had to move to the Hacker-Bright Clini RF, a floor model and , of course, the cost is significantly higher. William DeSalvo, B.S., HTL(ASCP) Date: Tue, 12 Jul 2011 19:16:59 -0500 From: techon...@comcast.net To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histobath revised Hey again. I was not refering to a tissue flotation bath. The Histobath was a product sold by Shandon. It had a chamber filled with isopentane or alcohol and was used for quick freezing tissue for a cryostat. Sorry if there was any confusion. Thanks Matt -- Matthew Mincer Tech One Biomedical Services 159 N Marion Street, PMB163 Oak Park, IL 60301 (708) 383-6040 X 10 fax (708) 383-6045 cell (708) 822-3738 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
