[Histonet] Re:
Sexual problems are to be solved... http://ravera.todouy.com/bestsite.php?opage=20f0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Research microtomes
This post is actually on behalf of some interns that are working at my company over the summer. Their emails are included on this post, so I'm going to turn it over to them:) But here is their description of what they're doing: In answer to Jack's questions (I thought about replying myself, but didn't want to confuse anyone), we want to make a CEMA array (cutting edge matrix assembly array), which could be done with a variety of tissue types. The step that calls for the special microtome is when you cut the original blocks from the hospital -- meaning variable tissue depth. Though the article mentioned a microtome that could cut 50-2000, we are most interested in the range around 50-150 microns. Thanks, Adrienne From: Jack Ratliff ratliffj...@hotmail.com To: Adrienne Anderson rennie1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; bush...@rose-hulman.edu bush...@rose-hulman.edu; mlosbo...@gmail.com mlosbo...@gmail.com Sent: Wednesday, July 20, 2011 6:13 PM Subject: Re: [Histonet] Research microtomes Couple more questions. :) What is the tissue and the dimensions of the specimen? On Jul 20, 2011, at 4:57 PM, Adrienne Anderson rennie1...@yahoo.com wrote: Hi Jack, We're trying to cut just plain old FFPE blocks. From: Jack Ratliff ratliffj...@hotmail.com To: Adrienne Anderson rennie1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; bush...@rose-hulman.edu bush...@rose-hulman.edu; mlosbo...@gmail.com mlosbo...@gmail.com Sent: Wednesday, July 20, 2011 5:02 PM Subject: Re: [Histonet] Research microtomes What type of specimen are you trying to cut? What embedding media are you using? Jack On Jul 20, 2011, at 2:48 PM, Adrienne Anderson rennie1...@yahoo.com wrote: Hello Histo-land, I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! Thanks, Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Research microtomes
Thanks a lot for your response Adrienne! I think I may be able to help you with this. Is there a time we can talk off-line over the phone? My number is 317-281-1975. Best Regards, Jack On Jul 21, 2011, at 9:49 AM, Adrienne Anderson rennie1...@yahoo.com wrote: This post is actually on behalf of some interns that are working at my company over the summer. Their emails are included on this post, so I'm going to turn it over to them:) But here is their description of what they're doing: In answer to Jack's questions (I thought about replying myself, but didn't want to confuse anyone), we want to make a CEMA array (cutting edge matrix assembly array), which could be done with a variety of tissue types. The step that calls for the special microtome is when you cut the original blocks from the hospital -- meaning variable tissue depth. Though the article mentioned a microtome that could cut 50-2000, we are most interested in the range around 50-150 microns. Thanks, Adrienne From: Jack Ratliff ratliffj...@hotmail.com To: Adrienne Anderson rennie1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; bush...@rose-hulman.edu bush...@rose-hulman.edu; mlosbo...@gmail.com mlosbo...@gmail.com Sent: Wednesday, July 20, 2011 6:13 PM Subject: Re: [Histonet] Research microtomes Couple more questions. :) What is the tissue and the dimensions of the specimen? On Jul 20, 2011, at 4:57 PM, Adrienne Anderson rennie1...@yahoo.com wrote: Hi Jack, We're trying to cut just plain old FFPE blocks. From: Jack Ratliff ratliffj...@hotmail.com To: Adrienne Anderson rennie1...@yahoo.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; bush...@rose-hulman.edu bush...@rose-hulman.edu; mlosbo...@gmail.com mlosbo...@gmail.com Sent: Wednesday, July 20, 2011 5:02 PM Subject: Re: [Histonet] Research microtomes What type of specimen are you trying to cut? What embedding media are you using? Jack On Jul 20, 2011, at 2:48 PM, Adrienne Anderson rennie1...@yahoo.com wrote: Hello Histo-land, I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! Thanks, Adrienne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Small Slide Scanner
Hi all. Is anyone out there using one of the small, low volume slide scanners for use in frozen sections? We have looked at a couple of the larger scanners for other purposes but wanted to know how the smaller ones would work for our pathologists to review frozen slides from off-site facilities. Of course, a PA would gross the specimen at the facility, cut the slides and then load them into the scanner. Any thoughts? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fast Blue stabilization
Hi List, We are retrogradely labeling spinal motoneurons with Fast Blue, which works well. When we process our sections (60-µm) for immunohistochemical labeling, the Fast Blue signal becomes much weaker. We believe that the detergents we are using to facilitate antibody penetration are also allowing the Fast Blue to leak out of the cells. Is anyone aware of methods for stabilizing Fast Blue within the cells so it won't wash out during immunohistochemical processing? Jonathan Carp, Ph.D. Wadsworth Center New York State Dept. Health Albany, NY IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotechnologist positions at George Washington University
I am posting the following position announcements for Dr. Patricia Latham at GWU, in Washington, DC. If you are interested in the positions, please contact her directly: plat...@mfa.gwu.edu. Thank you! One position is funded for development of a histopathology core lab in the GW School of Biomedical Sciences. This lab will be expected to provide tissue processing, sections, routine and special stains and immunostaining to meet the needs of researchers at GWU, primarily in the Biomedical sciences, but all departments will be invited to submit tissues. This person will need to work independently to deliver excellent quality results in a timely manner from the get-go. The person should have good managerial skills, since there will be a need to maintain inventory and to keep track of the flow of specimens and charges. Ideally, the person would have an entreprenurial spirit since the success of the lab will determine its future existence. The position is assured for 2 years, but it could grow to full-time, if the lab does well. The second position is not available quite yet (but very soon) and this person must also be a qualified histotechnologist with experience. This is a contract position at half-time to provide a service to a Pharma project - now scheduled through May 2015. The work involved for the contract will be very limited to a select number of routine stains and one immunostain. However, the person will need to be entering data into an audit-trail database and to maintain meticulous records and interdepartmental communications on a limited but international scale. There will be significant time not involved in the contract work for research projects that I intend to pursue. It would be ideal for someone wanting to take an advanced degree or to get involved in biomedical research. This person will be more like a research assistant, except for the contract obligations. Esther C. Peters, Ph.D. Assistant Professor Department of Environmental Science Policy George Mason University Fairfax, VA 22030- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RPM on Cytocentrifuge for CSF
How long and at what RPM do you process your CSF cytocentrifuge specimens? Thank you. Marcia Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center 1415 Ross Ave El Centro, CA 92243 760-339-7267 760-482-5365(F) www.ecrmc.orghttp://www.ecrmc.org/ Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. Â ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] University of Pittsburg
Hi Histonet, Can someone from the University of Pittsburg immunohistochemistry department please send me an email. One of the pathologists here wanted me to find out what you all do for Hep C. Do you perform the immuno or other? Thank you in advance. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Veterinary Histology
Greetings, My name is Kevin and I've been working in a pathology / clinical lab setting for the past 8 yrs. I've recently heard about veterinary histology and the possibilities seem endless. My initial searches have not been very productive and I've even heard that certain states do not allow such studies Is this true ? if so, which ones...? I currently live in Tallahassee, Florida and will be re-locating east ( Jacksonville - Daytona Beach, Fl ) in the near future. Can anyone help enlighten me ? Any and all help would be greatly appreciated. God Bless, Kevin R. Evelyn KWB Pathology Assoc Tallahassee, Florida ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: RPM on Cytocentrifuge for CSF
500rpm for 5 minutes using the Shandon Cytospin 3 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcia Fisher Sent: Friday, 22 July 2011 4:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RPM on Cytocentrifuge for CSF How long and at what RPM do you process your CSF cytocentrifuge specimens? Thank you. Marcia Fisher Histology Supervisor/Lab Safety Officer El Centro Regional Medical Center 1415 Ross Ave El Centro, CA 92243 760-339-7267 760-482-5365(F) www.ecrmc.orghttp://www.ecrmc.org/ Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 0.5% acid alcohol
any one has the procedure to make 0.5% acid alcohol ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] 0.5% acid alcohol
Dorothy, Usually made up in 70% ethanol using Hydrochloric acid ie: Ethanol 70ml Water 30ml HCl (Concentrated) 0.5ml But a recipe should be available for your 0.5% acid alcohol (since we are assuming HCL and 70% ethanol is used - could be any other acid or absolute rather than 70%) What is it to be used for? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dorothy Glass Sent: Friday, 22 July 2011 1:49 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] 0.5% acid alcohol any one has the procedure to make 0.5% acid alcohol ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
