RE: [Histonet] Microtome draft shield
negative pressure will make the ribbons have a flying party. is it feassible to built a room within the lab with no pressure for sectioning? mg From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni [trathbo...@somerset-healthcare.com] Sent: Tuesday, July 19, 2011 9:11 AM To: 'Keri Colwell'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Microtome draft shield Haven't seen anything that specific, but you might want to look at the various biohazard splash guards. They are a clear Plexiglas, and they have a base to support them. You would be able to move them around (or have them mounted to the counter if desired), and they come in an assortment of angles and sizes. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Keri Colwell Sent: Monday, July 18, 2011 5:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome draft shield Hi Everyone, I work in a lab which is under negative pressure (air is continuously flowing in), and due to the layout of the rooms are microtomes are located next to two different doorways. We are looking for some sort of draft shield to place around each microtome and water bath that will reduce the effects of the airflow and personnel movements on our ribbons. Anyone have any suggestions as to who might sell such a thing? Thanks in advance! Keri Colwell Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640 | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colw...@inspection.gc.ca Telephone | Téléphone: 403-382-5500 Facsimile | Télécopieur: 403-382-5583 Government of Canada | Gouvernement du Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE protocol question
Hi Histonetters! I'm looking for thoughts on preferences/pros/cons between using a progressive and a regressive HE on routine daily work. Which hematoxylins do you prefer (commercially prepared), which eosin? Anyone have a tried and true protocol for each method? Thanks! Michelle Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Invitation to connect on LinkedIn
LinkedIn Thomas Huynh requested to add you as a connection on LinkedIn: -- David, I'd like to add you to my professional network on LinkedIn. - Thomas Accept invitation from Thomas Huynh http://www.linkedin.com/e/yvpgd1-gqjdaufb-1q/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I136816395_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYRejcScjwScP59bQRLsDpOqkRVbP8QcjcOe3oRcPcLrCBxbOYWrSlI/EML_comm_afe/ View invitation from Thomas Huynh http://www.linkedin.com/e/yvpgd1-gqjdaufb-1q/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I136816395_13/3cNnPkVcPoNe3oPckALqnpPbOYWrSlI/svi/ -- DID YOU KNOW LinkedIn can help you find the right service providers using recommendations from your trusted network? Using LinkedIn Services, you can take the risky guesswork out of selecting service providers by reading the recommendations of credible, trustworthy members of your network. http://www.linkedin.com/e/yvpgd1-gqjdaufb-1q/svp/inv-25/ -- (c) 2011, LinkedIn Corporation ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Wax disposal
Are the disposal issues over the Used paraffin because of chemicals like xylene in the wax Or is it the human tissue in the Wax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Processing: Blue Dye used to see small tissue
We use Mucicarmine placed in a small dropper bottle, it does not wash out in the processor and makes the tissues easy to see once embedded. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Recycled alcohol
You do not get 100% alcohol back from recycling, so it cannot be used as such in either the processor or stainer. Basically, you have to use it as 95%. That may be your problem!! This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NBF Recycled Alc
Hi all, We dump everything (and I mean EVERYTHING) down the drain. Formalin, waste from Benchmark, etc. We use 95% recycled alcohol for everything as well (stainer, making up reagents, etc), but that's the only reagent (other than clear-rite) that we use recycled. Our 100% alcohol is right from the bottle. We check our 95 also, and if it's not a 'true' 95, it gets dumped. Thanks Amy R. Holy Spirit Health System 503 N. 21st Street Camp Hill, PA 17011 Phone: 717-763-2124 Fax: 717-763-2947 www.hsh.org Attention: This Message is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination or copying of this message or the taking of any action in reliance on the contents of this message is strictly prohibited. If you have received this message in error, please notify us immediately and destroy the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PARAFFIN WASTE
Our paraffin is also hauled off with hazardous waste Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 0216, Fax 812-482-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ki67/mart 1 double stain
I have a protocol I will share from the benchmark lt for dual stains if you want to e-mail me directly! Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 0216, Fax 812-482-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Frozen eye problems
Hello Histoneters, I'm having problems getting a good section on paraformaldyehyde fixed adult mouse eyes frozen in TBS tissue freezing medium. One of the problems was the orentation of them. They were too close to the edge. I also, wanted to try a harder freezing medium, so I corrected oriented and used an OCT equivalent tissue freezing medium. They looked better, but still not as nice as I think they should be. My eye contact tells me that they should be cut at -20 for adult and -14 for neonatal. They have me set the knife angle at 0 to -2.5. I also tried cutting these as I would cut any other frozen issue, but I'm just pulling my hair out. Does anyone have any other suggestions that I might try? Your help would be greatly appreciated. Bobbie Boyce Histology Specialist III duPont Hospital for Children Wilmington, DE 302-651-6771 (Lab) 302-651-5010 (Fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Frozen eye problems
Try Neg-50 (Fisher, cat#22-110-617) instead of OCT, we don't have any problem with fresh-frozen mouse eyes. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: agleiber...@cbiolabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyce, Bobbie Sent: Monday, July 25, 2011 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen eye problems Hello Histoneters, I'm having problems getting a good section on paraformaldyehyde fixed adult mouse eyes frozen in TBS tissue freezing medium. One of the problems was the orentation of them. They were too close to the edge. I also, wanted to try a harder freezing medium, so I corrected oriented and used an OCT equivalent tissue freezing medium. They looked better, but still not as nice as I think they should be. My eye contact tells me that they should be cut at -20 for adult and -14 for neonatal. They have me set the knife angle at 0 to -2.5. I also tried cutting these as I would cut any other frozen issue, but I'm just pulling my hair out. Does anyone have any other suggestions that I might try? Your help would be greatly appreciated. Bobbie Boyce Histology Specialist III duPont Hospital for Children Wilmington, DE 302-651-6771 (Lab) 302-651-5010 (Fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE protocol question
Progressive hematoxylins usually take more time to stain and for routine work the schedule has to be very fine tuned because otherwise obtaining the ideal staining is quite difficult with a frequent tendency to the under staining. Regressive hematoxylins on the other hand, always over stain and then you can obtain the ideal staining by removing the hematoxylin with acid or aqueous solutions. They are faster and the results are more easy to control. For many years I always prepared my hematoxylins, but now there are commercial solutions of great quality and consistent results so it is better (although no more cost effective) to buy a reputable brand, develop a good protocol and stick to it. At the end I relied on Richard Allen hematoxylins. Eosin, on the other hand, are so easy to prepare that I always prepared mine. René J. --- On Mon, 7/25/11, histot...@imagesbyhopper.com histot...@imagesbyhopper.com wrote: From: histot...@imagesbyhopper.com histot...@imagesbyhopper.com Subject: [Histonet] HE protocol question To: Histonet@Lists. Utsouthwestern. Edu histonet@lists.utsouthwestern.edu Date: Monday, July 25, 2011, 7:29 AM Hi Histonetters! I'm looking for thoughts on preferences/pros/cons between using a progressive and a regressive HE on routine daily work. Which hematoxylins do you prefer (commercially prepared), which eosin? Anyone have a tried and true protocol for each method? Thanks! Michelle Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DS MART 1/ KI 67
FOR BENNCHMARK LT Procedure XT IHC DS UDAB-URED Depar Cell condition short 8 min 37 degrees/default Mart 1 32 min Amplify ultrawash aby denaturation 90 deg C 4 min 37 degrees/default Ki67 4min DS ultra wash counterstain Heme (we do 20 min on this our Path likes it dark) usually Heme is 4 min Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-482-0210, 0216, Fax 812-482-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Working Ventana Probe Protocols (EBER)
Hi Marc and Histonetters (especially those using XTs), I've been trying to work up VMS's new EBER probe with less than stellar results. I have some questions about the protocols you sent via Histonet back in early June. My questions/protocols are in red. You state the following: Depar 16 min We can only select depar without an incubation timedoes your instrument allow you to select a time? We are doing this on an XT, is this an instrument difference? What instrument are you running your ISH onUltra by chance? Enzyme Protease 2 for 4 min. We are using ISH Protease 2 / 12 mins. as that was our protocol with the old stuff. ISH (EBER) probe 4 min. Same Denature @ 85 degrees for 12 min. Same Hybe 1 hour What temperature are you hybridizing at? 3 stringency washes for 8 minutes each At what temperature? Blue detection for 20 minutes Same Counterstain for 4 min We use Nuclear Fast Red for 8 mins. Marc, are you using HybReady? Anyone else having had some success with the new EBER probe, reagents and software, feel free to comment. Of the 3 specimens I've run, known positive soft tissue, bm bx and cell block, the soft tissue and bm bx had some positivity with the EBER DNP and U6 DNP but the cell block was negative with both. There was also lots of blue smearing and haze over the slides. I'm afraid this optimization/validation could end up being very expensive even with our 3 control tissues picked up together on single slides. Thanks for everyone's help, Linda Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Working Ventana Probe Protocols (EBER)
Hi Linda, I had simliar trouble before I bought and added the extra HybReady to the protocol (there is one that comes with the detection kit, but you need two for good staining). It will clean up your stain and A LOT and remove those blue splotches that show up when you don't use it. Mark On Mon, Jul 25, 2011 at 9:39 AM, Sebree Linda A lseb...@uwhealth.orgwrote: Hi Marc and Histonetters (especially those using XTs), I've been trying to work up VMS's new EBER probe with less than stellar results. I have some questions about the protocols you sent via Histonet back in early June. My questions/protocols are in red. You state the following: Depar 16 min We can only select depar without an incubation timedoes your instrument allow you to select a time? We are doing this on an XT, is this an instrument difference? What instrument are you running your ISH onUltra by chance? Enzyme Protease 2 for 4 min. We are using ISH Protease 2 / 12 mins. as that was our protocol with the old stuff. ISH (EBER) probe 4 min. Same Denature @ 85 degrees for 12 min. Same Hybe 1 hour What temperature are you hybridizing at? 3 stringency washes for 8 minutes each At what temperature? Blue detection for 20 minutes Same Counterstain for 4 min We use Nuclear Fast Red for 8 mins. Marc, are you using HybReady? Anyone else having had some success with the new EBER probe, reagents and software, feel free to comment. Of the 3 specimens I've run, known positive soft tissue, bm bx and cell block, the soft tissue and bm bx had some positivity with the EBER DNP and U6 DNP but the cell block was negative with both. There was also lots of blue smearing and haze over the slides. I'm afraid this optimization/validation could end up being very expensive even with our 3 control tissues picked up together on single slides. Thanks for everyone's help, Linda Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job opening Prescott Arizona
Just wanted to add a few details. We are currently searching for a Cana date for our opening. We are located in Northern Arizona in the pines. We have two Campuses with the main campus in Prescott with the second campus located in Prescott Valley. We are a total of Approx. 187 beds combined. Please Check out our web site at YRMC.org to see our job opening and information about our Hospital. Prescott Az on the web to check out the area Arizona is full of history. We are located 1 1/2 hours north of Phoenix and the same to Flagstaff. Sedona is about 45 mins away and the Grand Canyon 3 hours. Contact me here for any questions and if interested fill out an application on the YRMC web site. Thanks for your time. Jeff Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cutting frozen decalcified mouse bones
Hi all, I had a very difficult time cutting my very precious triple transgenic frozen decalcified mouse bones this morning. As I cut into them, the sections scrunched up into a mess of OCT pretty much as soon as the blades hit the block. These were 4% PFA perfused and post-fixed for 24 hours, decalcified with 14% EDTA using weight loss/weight gain as an endpoint, cryoprotected overnight in 30% sucrose, embedded in OCT using liquid nitrogen cooled isopentane vertically (i.e. I was cutting from one growth plate to the other), and cut at 7 uM. Changing to a new blade didn't help. Changing the cutting temperature from -10C to -15C didn't help. Changing the cutting speed didn't help. I can't change the cutting angle without a special tool, and this isn't my device so I don't want to mess with it. The only way I could get reasonable sections was to use a tape transfer system, which seemed like overkill to me, but I need these sections ASAP. Ideas? I have a whole bunch more bones I need to cut over the next few weeks... Thanks, Adam ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] blurry tissue
I would like some thoughts on how to resolve some blurry looking tissue. We have had occasional tissue that looks blurry and not crisp for several weeks now. It is not all the cases only random tissues. The tissue is not on the same tissue processor either. We have 2 processors. The latest cases were a breast, some skins, and a prostate. I am not certain if this is happening on the tissue processor or in the stainer. It has been very humid in our lab so I have started running a dehumidifier in case there is water in the xylene. It is so hit and miss that I am puzzled. Any suggestions would be greatly appreciated. Thank you in advance for your thoughts. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Biogenex Xmatrx Infinity Stainer
Hi Everyone! I was wondering if anyone out there had and was using the Biogenex Xmatrx Infinity Stainer from Biogenex. We are considering buying one to do ISH and FISH on, but was wondering what other people had to say about it. Any feedback would be greatly appreciated! Thanks! Anna Hughes Merck Co., Inc. West Point, PA Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Formalin down the drain??
I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything (and I mean everything) from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, Hazardous materials in the histopathology laboratory by Janet Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Antibody suggestions for dog and cat
Could anyone suggest suituble antibodies for the following markers in dog and cat tissue: Vimentin, Pankeratin, CD3 and CD20, CD18, MelanA, Factor VIII Also could use a suggestion for C-kit in Dog tissue only. thanks Mark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cerner CoPath Plus
Anyone using this system, would you please contact me off line? I have questions regarding the PicsPlus component as well as I'm curious to see how everyone likes it. Thanks! j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Antibody suggestions for dog and cat
Hi Mark, Vimentin - Dako; M0725, clone V9 Pankeratin - Dako; M0821; clone MNF116 CD3 - LabVision; RB-9039; rabbit polyclonal CD20 - LabVision; RB-9013; rabbit polyclonal CD18 (canine) - Leukocyte Antigen Biology Lab (UC-Davis; Dr. Peter Moore); clone CA16.3C10 CD18 (feline) - Leukocyte Antigen Biology Lab (UC-Davis; Dr. Peter Moore); clone FE3.9F2 Melan A - Dako; M7195; clone A103 Factor VIII - Dako; A0082; rabbit polyclonal CD117 - Dako; A4502; rabbit polyclonal (not specific for dogs; tested successfully on cow and horse, also) Let me know if you need information on any other antibodies in question. I species-validate all of my IHC tests (100+). Jan Shivers Senior Scientist Histology/IHC/EM Section Head Pathology Teaching Program University of Minnesota Veterinary Diagnostic Laboratory 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu (Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.) - Original Message - From: Mark Tarango marktara...@gmail.com To: histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Sent: Monday, July 25, 2011 1:33 PM Subject: [Histonet] Antibody suggestions for dog and cat Could anyone suggest suituble antibodies for the following markers in dog and cat tissue: Vimentin, Pankeratin, CD3 and CD20, CD18, MelanA, Factor VIII Also could use a suggestion for C-kit in Dog tissue only. thanks Mark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] blurry tissue
What is the staining intensity like? Thanks, Nacaela Johnson, B.S. HTL (ASCP)CM Histotechnologist KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Monday, July 25, 2011 12:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] blurry tissue I would like some thoughts on how to resolve some blurry looking tissue. We have had occasional tissue that looks blurry and not crisp for several weeks now. It is not all the cases only random tissues. The tissue is not on the same tissue processor either. We have 2 processors. The latest cases were a breast, some skins, and a prostate. I am not certain if this is happening on the tissue processor or in the stainer. It has been very humid in our lab so I have started running a dehumidifier in case there is water in the xylene. It is so hit and miss that I am puzzled. Any suggestions would be greatly appreciated. Thank you in advance for your thoughts. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet /preThe contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.brOnly the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone./pre ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Antibody suggestions for dog and cat
Hello Mark We use the Ventana Benchmark for most of our IHC. We don't use all of the markers you listed, but and for our canine and feline tissue we do have great results with the following: Vimentin-Ventana Cytokeratin-Dako CD18-UC-Davis (Dr. Peter Moore) C-kit-Dako We worked hard to get the Melan A (Dako) working but had trouble with using a DAB detection kit..perhaps a red kit would be better. If you would like more info, feel free to contact me directly. Kathy Kathleen Jones Research Technologist Pathology/Microbiology AVC - UPEI (902)566-0595 Mark Tarango marktara...@gmail.com 25/07/2011 3:33 PM Could anyone suggest suituble antibodies for the following markers in dog and cat tissue: Vimentin, Pankeratin, CD3 and CD20, CD18, MelanA, Factor VIII Also could use a suggestion for C-kit in Dog tissue only. thanks Mark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for assistance in Southeast part of the country-
Hello- Is there anyone in the southeast part of the country with familiarity with the: Shandon Hypercenter XP tissue processor? Leica ST5050 IHC stainer (Jung Immunostainer)? I was looking for someone with experience with either of these that may be interested and able to pass along some knowledge to 'new' users of the instruments. Thank you in advance- Regards- Jon Arnold T: 1-800-745-2710 | F: 763-712-8724 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin down the drain??
Michael, Since this seems to come up somewhat regularly, if you do have a link to the federal laws which govern this, maybe you could share. I'm sure this would help labs get the support they need for proper disposal. Toni -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mtitf...@aol.com Sent: Monday, July 25, 2011 1:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything (and I mean everything) from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, Hazardous materials in the histopathology laboratory by Janet Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need a protocol for CD24 and CD133
Hi. Does anyone out there have a protocol for CD24 and CD133 that will work on mouse Xenograft tumors? Preferably a protocol that will work on Leica Bond, but any working protocol will do at this point. Thank you and have a good day. Dusko ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Biocare decloaker
We are trying to validate a Biocare decloaker and have found when we use 122 degrees for 30 seconds we get great signal, but distorted morphology. If we reduce to 90 degrees for 45 minutes, the signal is significantly decreased but the morphology is good. What protocols are you using? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin down the drain??
One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the American Revolution) Some states are more heavily regulated than others. California and Colorado come to mind immediately. Different organizations, locations and circumstances may allow for disposal of products that may be diluted to such a degree as to be negligable in the waste stream. Our institution generates 65,000 gallons of waste water daily, which allows us to make the dilution limits of anything that our histo lab could produce in a day. No laws are broken if I should pour xylene, formalin, alcohols or other common compounds that we might generate on even our busiest days into the waste stream. HOWEVER, while we may be allowed to do so by state and local regulations, we have decided it is not prudent to do so and so we collect, ship, neutralize or recycle most all that the histo lab generates. We do this at the lab level, with lab funding. It is the responsible thing to do, and we are morally and ethically bound to do so, but we are not outside the law if we do not. If your local municipal waste systems people give you the green light on dumping formalin down the drain. you are not breaking the law, federal or otherwise, in doing so. It is true that if you wish to affect things globally, one has to be responsible locally. Here is what my rant comes down to Make certain that you are meeting local standards for your chemical disposal or you may well be breaking the law. And a big thank you (from myself, my children, grandchildren and great-grand children and that lady who sells me the slurpee at the local convenience store) for anything anyone is doing above and beyond that. :)Rant is over... Have a nice day :) You cannot Like this rant on Facebook or follow this rant on Twitter. Bill -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mtitf...@aol.com Sent: Monday, July 25, 2011 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything (and I mean everything) from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, Hazardous materials in the histopathology laboratory by Janet Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin down the drain??
I may not be able to Like Bill's rant, but I second it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Monday, July 25, 2011 4:20 PM To: mtitf...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin down the drain?? One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the American Revolution) Some states are more heavily regulated than others. California and Colorado come to mind immediately. Different organizations, locations and circumstances may allow for disposal of products that may be diluted to such a degree as to be negligable in the waste stream. Our institution generates 65,000 gallons of waste water daily, which allows us to make the dilution limits of anything that our histo lab could produce in a day. No laws are broken if I should pour xylene, formalin, alcohols or other common compounds that we might generate on even our busiest days into the waste stream. HOWEVER, while we may be allowed to do so by state and local regulations, we have decided it is not prudent to do so and so we collect, ship, neutralize or recycle most all that the histo lab generates. We do this at the lab level, with lab funding. It is the responsible thing to do, and we are morally and ethically bound to do so, but we are not outside the law if we do not. If your local municipal waste systems people give you the green light on dumping formalin down the drain. you are not breaking the law, federal or otherwise, in doing so. It is true that if you wish to affect things globally, one has to be responsible locally. Here is what my rant comes down to Make certain that you are meeting local standards for your chemical disposal or you may well be breaking the law. And a big thank you (from myself, my children, grandchildren and great-grand children and that lady who sells me the slurpee at the local convenience store) for anything anyone is doing above and beyond that. :)Rant is over... Have a nice day :) You cannot Like this rant on Facebook or follow this rant on Twitter. Bill -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mtitf...@aol.com Sent: Monday, July 25, 2011 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything (and I mean everything) from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, Hazardous materials in the histopathology laboratory by Janet Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Where Can I Buy Pneumo Control Slides
Good Afternoon to All, I am getting pretty desperate to find a source for Pneumo control slides. I know they are hard to come by, but my favorite source Newcomer Supply does not have any and I ordered some from Sigma-Aldrich and now they are discontinued. Please Help! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Biocare decloaker
There is a huge difference between 122 degrees for 30 seconds and 90 degrees for 45 minutes. I would say you need to look at something here! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Monday, July 25, 2011 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare decloaker We are trying to validate a Biocare decloaker and have found when we use 122 degrees for 30 seconds we get great signal, but distorted morphology. If we reduce to 90 degrees for 45 minutes, the signal is significantly decreased but the morphology is good. What protocols are you using? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Biocare decloaker
As to the efficacy of retrieval, there is actually not that big of a difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent to 125 degrees for 30 seconds. My guess, based on limited information in the original email is that Diana needs a new gasket. Those should be replaced every 6 months. Will Chappell Sent from my iPhone On Jul 25, 2011, at 1:34 PM, Mike Pence mpe...@grhs.net wrote: There is a huge difference between 122 degrees for 30 seconds and 90 degrees for 45 minutes. I would say you need to look at something here! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Monday, July 25, 2011 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare decloaker We are trying to validate a Biocare decloaker and have found when we use 122 degrees for 30 seconds we get great signal, but distorted morphology. If we reduce to 90 degrees for 45 minutes, the signal is significantly decreased but the morphology is good. What protocols are you using? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Biocare decloaker
I wonder if a new gasket would help me.. I keep blowing mine! :) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William Sent: Monday, July 25, 2011 16:40 To: Mike Pence Cc: histonet@lists.utsouthwestern.edu; Diana McCaig Subject: Re: [Histonet] Biocare decloaker As to the efficacy of retrieval, there is actually not that big of a difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent to 125 degrees for 30 seconds. My guess, based on limited information in the original email is that Diana needs a new gasket. Those should be replaced every 6 months. Will Chappell Sent from my iPhone On Jul 25, 2011, at 1:34 PM, Mike Pence mpe...@grhs.net wrote: There is a huge difference between 122 degrees for 30 seconds and 90 degrees for 45 minutes. I would say you need to look at something here! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Monday, July 25, 2011 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare decloaker We are trying to validate a Biocare decloaker and have found when we use 122 degrees for 30 seconds we get great signal, but distorted morphology. If we reduce to 90 degrees for 45 minutes, the signal is significantly decreased but the morphology is good. What protocols are you using? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] blurry tissue
You just pointed out to the most likely causes: happening during staining, high humidity and water in the xylene. Try to take care of these issues and you will resolve the problem. René J. --- On Mon, 7/25/11, Carol Bryant cb...@lexclin.com wrote: From: Carol Bryant cb...@lexclin.com Subject: [Histonet] blurry tissue To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Monday, July 25, 2011, 1:42 PM I would like some thoughts on how to resolve some blurry looking tissue. We have had occasional tissue that looks blurry and not crisp for several weeks now. It is not all the cases only random tissues. The tissue is not on the same tissue processor either. We have 2 processors. The latest cases were a breast, some skins, and a prostate. I am not certain if this is happening on the tissue processor or in the stainer. It has been very humid in our lab so I have started running a dehumidifier in case there is water in the xylene. It is so hit and miss that I am puzzled. Any suggestions would be greatly appreciated. Thank you in advance for your thoughts. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Biocare decloaker
Ok that is a bigger problem and a very clear answer to your staining issue. Your decloaker should never blow a gasket. Something is wrong and you probably need service or a replacement. Your decloaker is probably getting too hot and over retrieving tissue. I suggest immediately calling Biocare technical support. You need this fixed! Will Chappell Sent from my iPhone On Jul 25, 2011, at 1:42 PM, Weems, Joyce jwe...@sjha.org wrote: I wonder if a new gasket would help me.. I keep blowing mine! :) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William Sent: Monday, July 25, 2011 16:40 To: Mike Pence Cc: histonet@lists.utsouthwestern.edu; Diana McCaig Subject: Re: [Histonet] Biocare decloaker As to the efficacy of retrieval, there is actually not that big of a difference. Biocare publishes that 90 degrees for 60 mins is roughly equivalent to 125 degrees for 30 seconds. My guess, based on limited information in the original email is that Diana needs a new gasket. Those should be replaced every 6 months. Will Chappell Sent from my iPhone On Jul 25, 2011, at 1:34 PM, Mike Pence mpe...@grhs.net wrote: There is a huge difference between 122 degrees for 30 seconds and 90 degrees for 45 minutes. I would say you need to look at something here! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Monday, July 25, 2011 2:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare decloaker We are trying to validate a Biocare decloaker and have found when we use 122 degrees for 30 seconds we get great signal, but distorted morphology. If we reduce to 90 degrees for 45 minutes, the signal is significantly decreased but the morphology is good. What protocols are you using? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin down the drain??
Your rant is interesting but wrong. OSHA (which is a FEDERAL agency) prohibits dumping ANY type of hazardous materials down the drain. I was also taken aback by Amy's posting. No, regardless of what your state law may or may not permit you to dump in the drain, you should not put some $avings over the well being of the environment and the drinking water of people. Formaldehyde is toxic and recently officially declared carcinogen. In the same way that frackting methods to obtain gas from shale has been deemed dangerous, equally dumping formaldehyde, xylene and any other chemical ought to be the source of concern. This in my rant! René J. --- On Mon, 7/25/11, O'Donnell, Bill billodonn...@catholichealth.net wrote: From: O'Donnell, Bill billodonn...@catholichealth.net Subject: RE: [Histonet] Formalin down the drain?? To: mtitf...@aol.com, histonet@lists.utsouthwestern.edu Date: Monday, July 25, 2011, 4:19 PM One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the American Revolution) Some states are more heavily regulated than others. California and Colorado come to mind immediately. Different organizations, locations and circumstances may allow for disposal of products that may be diluted to such a degree as to be negligable in the waste stream. Our institution generates 65,000 gallons of waste water daily, which allows us to make the dilution limits of anything that our histo lab could produce in a day. No laws are broken if I should pour xylene, formalin, alcohols or other common compounds that we might generate on even our busiest days into the waste stream. HOWEVER, while we may be allowed to do so by state and local regulations, we have decided it is not prudent to do so and so we collect, ship, neutralize or recycle most all that the histo lab generates. We do this at the lab level, with lab funding. It is the responsible thing to do, and we are morally and ethically bound to do so, but we are not outside the law if we do not. If your local municipal waste systems people give you the green light on dumping formalin down the drain. you are not breaking the law, federal or otherwise, in doing so. It is true that if you wish to affect things globally, one has to be responsible locally. Here is what my rant comes down to Make certain that you are meeting local standards for your chemical disposal or you may well be breaking the law. And a big thank you (from myself, my children, grandchildren and great-grand children and that lady who sells me the slurpee at the local convenience store) for anything anyone is doing above and beyond that. :)Rant is over... Have a nice day :) You cannot Like this rant on Facebook or follow this rant on Twitter. Bill -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mtitf...@aol.com Sent: Monday, July 25, 2011 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything (and I mean everything) from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, Hazardous materials in the histopathology laboratory by Janet Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin down the drain??
I totally agree, under any circumstances, whatever the State law defines, we should not dump formalin, xylene or alcohols down the drain. And even though California has very strict rules, it even seems to differ from county to county. In one of my past jobs we were allowed to dump the formalin down the drain (Orange County of all places), whereas in San Diego, that was absolutely prohibited. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Monday, July 25, 2011 1:20 PM To: mtitf...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin down the drain?? One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the American Revolution) Some states are more heavily regulated than others. California and Colorado come to mind immediately. Different organizations, locations and circumstances may allow for disposal of products that may be diluted to such a degree as to be negligable in the waste stream. Our institution generates 65,000 gallons of waste water daily, which allows us to make the dilution limits of anything that our histo lab could produce in a day. No laws are broken if I should pour xylene, formalin, alcohols or other common compounds that we might generate on even our busiest days into the waste stream. HOWEVER, while we may be allowed to do so by state and local regulations, we have decided it is not prudent to do so and so we collect, ship, neutralize or recycle most all that the histo lab generates. We do this at the lab level, with lab funding. It is the responsible thing to do, and we are morally and ethically bound to do so, but we are not outside the law if we do not. If your local municipal waste systems people give you the green light on dumping formalin down the drain. you are not breaking the law, federal or otherwise, in doing so. It is true that if you wish to affect things globally, one has to be responsible locally. Here is what my rant comes down to Make certain that you are meeting local standards for your chemical disposal or you may well be breaking the law. And a big thank you (from myself, my children, grandchildren and great-grand children and that lady who sells me the slurpee at the local convenience store) for anything anyone is doing above and beyond that. :)Rant is over... Have a nice day :) You cannot Like this rant on Facebook or follow this rant on Twitter. Bill -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mtitf...@aol.com Sent: Monday, July 25, 2011 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything (and I mean everything) from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, Hazardous materials in the histopathology laboratory by Janet Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Where Can I Buy Pneumo Control Slides
American MasterTech sells them: http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStylesite m=CSP015P -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of wanda.sm...@hcahealthcare.com Sent: Monday, July 25, 2011 3:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Where Can I Buy Pneumo Control Slides Good Afternoon to All, I am getting pretty desperate to find a source for Pneumo control slides. I know they are hard to come by, but my favorite source Newcomer Supply does not have any and I ordered some from Sigma-Aldrich and now they are discontinued. Please Help! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Formalin down the drain??
Indeed not only county to county but your local municipality can have specific regulations related to hazardous waste discard in the sewer since it is their waste treatment plant that takes the hit so to speak. Most small municipalities will adopt state or county regulations (whichever is stricter) but they can always write even stricter rules if they wish and that may well be the case in some larger metro areas. Good luck. Joe University of Iowa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Monday, July 25, 2011 3:56 PM To: 'O'Donnell, Bill'; mtitf...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin down the drain?? I totally agree, under any circumstances, whatever the State law defines, we should not dump formalin, xylene or alcohols down the drain. And even though California has very strict rules, it even seems to differ from county to county. In one of my past jobs we were allowed to dump the formalin down the drain (Orange County of all places), whereas in San Diego, that was absolutely prohibited. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 1896 Rutherford Road Carlsbad, CA 92008 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Monday, July 25, 2011 1:20 PM To: mtitf...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Formalin down the drain?? One should not automtically assume that laws are broken here. (Rant begins here) First of all, it is the States that set the limits of what can and cannot be dumped. All States must meet Federal standards,but States are free to determine how they do that. (It's one of the benefits of the American Revolution) Some states are more heavily regulated than others. California and Colorado come to mind immediately. Different organizations, locations and circumstances may allow for disposal of products that may be diluted to such a degree as to be negligable in the waste stream. Our institution generates 65,000 gallons of waste water daily, which allows us to make the dilution limits of anything that our histo lab could produce in a day. No laws are broken if I should pour xylene, formalin, alcohols or other common compounds that we might generate on even our busiest days into the waste stream. HOWEVER, while we may be allowed to do so by state and local regulations, we have decided it is not prudent to do so and so we collect, ship, neutralize or recycle most all that the histo lab generates. We do this at the lab level, with lab funding. It is the responsible thing to do, and we are morally and ethically bound to do so, but we are not outside the law if we do not. If your local municipal waste systems people give you the green light on dumping formalin down the drain. you are not breaking the law, federal or otherwise, in doing so. It is true that if you wish to affect things globally, one has to be responsible locally. Here is what my rant comes down to Make certain that you are meeting local standards for your chemical disposal or you may well be breaking the law. And a big thank you (from myself, my children, grandchildren and great-grand children and that lady who sells me the slurpee at the local convenience store) for anything anyone is doing above and beyond that. :)Rant is over... Have a nice day :) You cannot Like this rant on Facebook or follow this rant on Twitter. Bill -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mtitf...@aol.com Sent: Monday, July 25, 2011 12:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formalin down the drain?? I was a little distressed to read the message from Amy in Camp Hill, Pennsylvania declaring she dumps everything (and I mean everything) from her histology lab down the drain. There are a bunch of Federal Laws governing handling and disposal of chemicals used in the histology laboratory and she appears to be breaking several. The wastewater law limits how much formalin you can discard down the sink (and you cannot dilute as you go). The same law forbids disposal of organic solvents like xylene, or solutions containing organic solvents. Local laws in Pennsylvania may be more strict. I recommend to Amy that she purchases a book like, Hazardous materials in the histopathology laboratory by Janet Richard Dapson and read the whole thing cover to cover! Michael Titford Pathology USA Mobile AL USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list
[Histonet] RE: Histonet Digest, Vol 92, Issue 33
This sounds to me like a processing issue, especially with the types of tissues you describe. Are the tissue sections you are referring to very thick? Maybe the tissue is too big for the amount of time in the different processing stations. I have also seen this happen when the tissue has been left to dry out before the clinician puts it into the formalin. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico -- Message: 2 Date: Mon, 25 Jul 2011 13:42:25 -0400 From: Carol Bryant cb...@lexclin.com Subject: [Histonet] blurry tissue To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 50DA0C6B72976B4AB3A0FCA04CC73DBF141CC36F8E@EXCHANGESB Content-Type: text/plain; charset=us-ascii I would like some thoughts on how to resolve some blurry looking tissue. We have had occasional tissue that looks blurry and not crisp for several weeks now. It is not all the cases only random tissues. The tissue is not on the same tissue processor either. We have 2 processors. The latest cases were a breast, some skins, and a prostate. I am not certain if this is happening on the tissue processor or in the stainer. It has been very humid in our lab so I have started running a dehumidifier in case there is water in the xylene. It is so hit and miss that I am puzzled. Any suggestions would be greatly appreciated. Thank you in advance for your thoughts. Carol Bryant, CT (ASCP) Cytology/Histology Manager Pathology Services Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Where Can I Buy Pneumo Control Slides
American Master Tech. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of wanda.sm...@hcahealthcare.com Sent: Monday, July 25, 2011 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Where Can I Buy Pneumo Control Slides Good Afternoon to All, I am getting pretty desperate to find a source for Pneumo control slides. I know they are hard to come by, but my favorite source Newcomer Supply does not have any and I ordered some from Sigma-Aldrich and now they are discontinued. Please Help! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Where Can I Buy Pneumo Control Slides
Statlab sells pneumo controls and they are in human tissues. Thanks Debbie Siena HT(ASCP)QIHC Technical Manager | StatLab Medical Products 407 Interchange St. | McKinney, TX 75071 Direct: 972-436-1010 x229 | Fax: 972-436-1369 dsi...@statlab.com | www.statlab.com - Original Message - From: Laurie Colbert [mailto:laurie.colb...@huntingtonhospital.com] Sent: Monday, July 25, 2011 04:21 PM To: wanda.sm...@hcahealthcare.com wanda.sm...@hcahealthcare.com; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Where Can I Buy Pneumo Control Slides American Master Tech. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of wanda.sm...@hcahealthcare.com Sent: Monday, July 25, 2011 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Where Can I Buy Pneumo Control Slides Good Afternoon to All, I am getting pretty desperate to find a source for Pneumo control slides. I know they are hard to come by, but my favorite source Newcomer Supply does not have any and I ordered some from Sigma-Aldrich and now they are discontinued. Please Help! Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE protocol question
Hi Michelle I have used both and my experience either is suitable for a good HE if they are used appropriately. For regressive staining you just need to optimise the time of haematoxylin staining with the concentration and time of the differentiation step. In a previous position I used Mayer's which is a progressive stain. We stained for 4 minutes in our HE protocol however to get the best staining we gave it a whiff of differentiation (1 min with 0.025% Acid alcohol) This was not to differentiate the nuclear staining but we found to get optimal nuclear staining the time required would result in some tissue types adopting a very pale blue haze in the background and removing this provided much better differential eosin staining. For counterstaining special stains and IHC the Mayer's was by far the best I have used. 1 minute would provide delicate nuclear staining with a clear background. This stain would not interfere with any other staining including nuclear staining for ER, PR, TTF1 etc. Good bluing was achieved simply with a good wash in our quite basic tap water. So I would recommend either for HE but progressive for a counterstain. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory _ Clinical and Statewide Services Division| QueenslandHealth Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ histot...@imagesbyhopper.com histot...@imagesbyhopper.com 7/25/2011 9:29 pm Hi Histonetters! I'm looking for thoughts on preferences/pros/cons between using a progressive and a regressive HE on routine daily work. Which hematoxylins do you prefer (commercially prepared), which eosin? Anyone have a tried and true protocol for each method? Thanks! Michelle Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] How hot is too hot for storing tissue blocks?
Hi folks, We were hoping someone could recommend a temperature range acceptable for storing tissue blocks? Also do you folks use a regular thermometer or a temperature chart recorder (records on graph) to record room temps? We are going to move to a new location and need help with the fine details. Thanks in advance, Hugh Hawaii ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
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