[Histonet] Opening in KC, Missouri
Full time or part time opening in Kansas City, Missouri for a busy Dermatopathology lab. Must be HT or HTL certified. Proficient in all aspects of routine histology. Grossing, coversliping, accessioning, microtonomy, and HE stains. Please call Tami at 816.584.8100 for immediate consideration. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Benchmark Ultra-Red Problems
We have been having an issue lately with a precipitate (possibly bacterial contaminant) using the Ultra View Red Detection Kit that is not showing up on stains using the Ultra View DAB Detection Kit. The red speckles appear on the tissue and also on the glass away from the tissue. We have alerted tech support, done several decontaminations and changed our water from purified to distilled. Has anyone experienced similar problems with the new formulation of the Ultra Red Detection kit or any other similar situations? Any thoughts on the subject would be greatly appreciated. Benchmark Ultra stainer I appreciate your input, Susan KDL Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana Benchmark Ultra-Red Problems
Have you tried using a different lot of the Ultra View Red... Ours has been working fine, so maybe you got a bad lot? Drew Sent from my iPhone On Aug 1, 2011, at 12:18 PM, Susan Foreman sfore...@labpath.com wrote: We have been having an issue lately with a precipitate (possibly bacterial contaminant) using the Ultra View Red Detection Kit that is not showing up on stains using the Ultra View DAB Detection Kit. The red speckles appear on the tissue and also on the glass away from the tissue. We have alerted tech support, done several decontaminations and changed our water from purified to distilled. Has anyone experienced similar problems with the new formulation of the Ultra Red Detection kit or any other similar situations? Any thoughts on the subject would be greatly appreciated. Benchmark Ultra stainer I appreciate your input, Susan KDL Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana Benchmark Ultra-Red Problems
We have tried both lots of the Ultra View Red -Original Message- From: Drew Meyer [mailto:41dm...@gmail.com] Sent: Monday, August 01, 2011 12:27 PM To: Susan Foreman Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana Benchmark Ultra-Red Problems Have you tried using a different lot of the Ultra View Red... Ours has been working fine, so maybe you got a bad lot? Drew Sent from my iPhone On Aug 1, 2011, at 12:18 PM, Susan Foreman sfore...@labpath.com wrote: We have been having an issue lately with a precipitate (possibly bacterial contaminant) using the Ultra View Red Detection Kit that is not showing up on stains using the Ultra View DAB Detection Kit. The red speckles appear on the tissue and also on the glass away from the tissue. We have alerted tech support, done several decontaminations and changed our water from purified to distilled. Has anyone experienced similar problems with the new formulation of the Ultra Red Detection kit or any other similar situations? Any thoughts on the subject would be greatly appreciated. Benchmark Ultra stainer I appreciate your input, Susan KDL Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC service ??
Can you tell me what kind of on-site technical service you are accustomed to receiving from Ventana (especially if you NOT in a big city so travel is required)? We have had a terrible time getting a Field service engineer into our lab this year and wonder if that is the norm for Ventana these days? For the Dako users - what kind of service are you getting? Thank you in advance Anna anna.in...@stmarygj.org CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana Benchmark Ultra-Red Problems
Is it appearing on every slide run with that detection regardless of the antibody? What are you using to remove the liquid coverslip? If it's dish-soap, did you just recently change brands or use one with a different formula? Drew Sent from my iPhone On Aug 1, 2011, at 12:24 PM, Susan Foreman sfore...@labpath.com wrote: We have tried both lots of the Ultra View Red -Original Message- From: Drew Meyer [mailto:41dm...@gmail.com] Sent: Monday, August 01, 2011 12:27 PM To: Susan Foreman Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana Benchmark Ultra-Red Problems Have you tried using a different lot of the Ultra View Red... Ours has been working fine, so maybe you got a bad lot? Drew Sent from my iPhone On Aug 1, 2011, at 12:18 PM, Susan Foreman sfore...@labpath.com wrote: We have been having an issue lately with a precipitate (possibly bacterial contaminant) using the Ultra View Red Detection Kit that is not showing up on stains using the Ultra View DAB Detection Kit. The red speckles appear on the tissue and also on the glass away from the tissue. We have alerted tech support, done several decontaminations and changed our water from purified to distilled. Has anyone experienced similar problems with the new formulation of the Ultra Red Detection kit or any other similar situations? Any thoughts on the subject would be greatly appreciated. Benchmark Ultra stainer I appreciate your input, Susan KDL Pathology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cresyl violet stain on 50 um mouse brain sections
Hi, we do this stain all the time in our lab. We only use gelatin coated slides. You can purchase them or you can coat them yourselves. Our sections were doing the same thing until we started using the gelatin coated slides. We no longer have that problem. I hope this helps! I remain yours truely, Candice Camille From: Michael, Susan micha...@janelia.hhmi.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Friday, July 29, 2011 8:11 AM Subject: [Histonet] Cresyl violet stain on 50 um mouse brain sections I am having trouble keeping my sections on the slides when I stain with cresyl violet. These are fixed frozen sections, 50 um, dried overnight on plus slides, overnight in 1 to 1 alcohol/chloroform, then rehydrated through 100, 95 ETOHs then to water. Into the cresyl violet solution, then 100% to 95% ETOH. Is it the water? Is it the slides? Any suggestions? Susan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mouse vs. Human cells
Hello Histo-hotties! Question: We are working with xenograft tumors in mice. The tumor cells are human in origin. I am trying to come up with some kind of stain that will stain all the mouse cells and none of the human cells. Just to be able to determine if any of the human tumor cells are in a normal looking say liver. I was thinking maybe Ki-67? It doesn't have to be any specific marker, just to be able to see a really blue mouse organ, and then if there are human cells of any type in the organ light up that one cell. Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Oops
That was backwards =) Don't stain mouse cells...stain human ones Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] C4d nuclear staining?
Has anyone ever noticed any nuclear staining with C4d in a renal biopsy? The control stains great, but the patient's tissue exhibits some nuclear staining in what look to be inflammatory cells. There has been a report of granular and some nuclear staining in lung transplants on structures beyond the vasculature but we haven't seen anything like this in a case of renal rejection. Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.NationwideChildrens.org One person with passion is better than forty people merely interested. ~ E.M. Forster - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thanks to Leica service
Quick note for those who may be interested. I received a message from Leica's service department offering to help with my blade holder issues. Hopefully we can get the problem resolved quickly. Thanks Leica ! Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
