[Histonet] unstained slides
Hello Histonetters, Recently we have seen an increase number of unstained slides requested from outside facilities. We do not release our blocks, so unstained slides are the only option for any facility to obtain our tissue. Currently we do not charge for these slides, but I am rethinking that policy. What is everyone doing about unstained slide requests? Are you charging for the slides? How much per slide? Any information would be helpful. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 Ext. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] unstained slides
Cindy: If these outside facilities are commercial labs doing proprietary testing, many of them will pay a set fee to your laboratory for preparing the slides. This needs to be negotiated with the commercial lab rep, but may be in the $40-50 range Lester J. Raff, MD Medical Director UroPartners Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel 708.486.0076 Fax 708.492.0203 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, August 17, 2011 7:02 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] unstained slides Hello Histonetters, Recently we have seen an increase number of unstained slides requested from outside facilities. We do not release our blocks, so unstained slides are the only option for any facility to obtain our tissue. Currently we do not charge for these slides, but I am rethinking that policy. What is everyone doing about unstained slide requests? Are you charging for the slides? How much per slide? Any information would be helpful. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories 716-250-9235 Ext. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Creative Waste Solutions
Does anyone know of a distributor of Creative Waste Solutions products? ___ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. ___ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Creative Waste Solutions
I may be wrong about this, but I don't think Rex has anyone else distributing his stuff yet. However, some of the stuff they sell is merely products that they relabel to distribute themselves. So, it depends on what specific products you're talking about. Drew Sent from my iPad On Aug 17, 2011, at 9:42 AM, Anita Buchiane abuchi...@bmhvt.org wrote: Does anyone know of a distributor of Creative Waste Solutions products? ___ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. ___ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Creative Waste Solutions
They sell there own supplies. We were just introduced to them by an independent salesperson. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anita Buchiane Sent: Wednesday, August 17, 2011 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Creative Waste Solutions Does anyone know of a distributor of Creative Waste Solutions products? ___ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. ___ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Creative Waste Solutions
Thank-You all for your responses. -Original Message- From: Sherwood, Margaret [mailto:msherw...@partners.org] Sent: Wednesday, August 17, 2011 10:07 AM To: Anita Buchiane; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Creative Waste Solutions They sell there own supplies. We were just introduced to them by an independent salesperson. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anita Buchiane Sent: Wednesday, August 17, 2011 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Creative Waste Solutions Does anyone know of a distributor of Creative Waste Solutions products? ___ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. ___ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. ___ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ordering special stain kits and control slides
Hi Histonetters, I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa. I do know the various vendors but wanted your input on who you use and why if possible. I also need to order HP control slides, same question.. Thanks in advance!! Jill Cox, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ordering special stain kits and control slides
Polyscientific RD have very good kits and great technical back up. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jill Cox Sent: Wednesday, August 17, 2011 9:16 AM To: Histonet@Lists. Edu Subject: [Histonet] Ordering special stain kits and control slides Hi Histonetters, I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa. I do know the various vendors but wanted your input on who you use and why if possible. I also need to order HP control slides, same question.. Thanks in advance!! Jill Cox, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ordering special stain kits and control slides
We use Poly Scientific www.PolyRnD.com Great support from their technical staff, timely deliveries, good quality. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jill Cox Sent: Wednesday, August 17, 2011 10:16 AM To: Histonet@Lists. Edu Subject: [Histonet] Ordering special stain kits and control slides Hi Histonetters, I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa. I do know the various vendors but wanted your input on who you use and why if possible. I also need to order HP control slides, same question.. Thanks in advance!! Jill Cox, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ordering special stain kits and control slides
We also use Poly Scientific. Lisa Children's Memorial Hospital Chg., IL -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, August 17, 2011 9:22 AM To: 'Jill Cox'; Histonet@Lists. Edu Subject: RE: [Histonet] Ordering special stain kits and control slides We use Poly Scientific www.PolyRnD.com Great support from their technical staff, timely deliveries, good quality. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jill Cox Sent: Wednesday, August 17, 2011 10:16 AM To: Histonet@Lists. Edu Subject: [Histonet] Ordering special stain kits and control slides Hi Histonetters, I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa. I do know the various vendors but wanted your input on who you use and why if possible. I also need to order HP control slides, same question.. Thanks in advance!! Jill Cox, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] aqueous mounting media
Depending on the staining you can use different mounting media. If it is only histological staining just mount the slides in 80% glycerol prepared in water or compatible buffer for the staining (whatever mounting media you use first test if it is compatable with your staining). If you have fluorescence staining then you may need to use anti-fade media. Most anti-fade media contain so called free radical (singlet oxygen species) scavenger chemicals that ara not compatible with all fluorescentdyes. You have to always test if the anti-fade mounting media is compatible with fluorescent dye you are using. Alternatively you may use Mowiol 4-88 from Calbiochem. Mowoil does not contain any free radical scavenger chemicals and prevents photobleaching, it compatible with almost all fluorescent dyes. Best, Mesruh Turkekul MSc, HTL(ASCP)QIHC turke...@mskcc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ordering special stain kits and control slides
I'm on the west coast and mainly use American Master Tech. They also have many histology products and great technical support. I also use Medical Chemical Corporation. Their prices are very good, and I can always get personalized assistance from Andy Rocha - he has bailed me out on several occasions. I'm not sure what control slides they carry, if any. Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jill Cox Sent: Wednesday, August 17, 2011 7:16 AM To: Histonet@Lists. Edu Subject: [Histonet] Ordering special stain kits and control slides Hi Histonetters, I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa. I do know the various vendors but wanted your input on who you use and why if possible. I also need to order HP control slides, same question.. Thanks in advance!! Jill Cox, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ordering special stain kits and control slides
Another vote for PolyScientific RD but Newcomer is also good and they also control slides. Andi Grantham On Aug 17, 2011, at 7:21 AM, Rathborne, Toni wrote: We use Poly Scientific www.PolyRnD.com Great support from their technical staff, timely deliveries, good quality. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jill Cox Sent: Wednesday, August 17, 2011 10:16 AM To: Histonet@Lists. Edu Subject: [Histonet] Ordering special stain kits and control slides Hi Histonetters, I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa. I do know the various vendors but wanted your input on who you use and why if possible. I also need to order HP control slides, same question.. Thanks in advance!! Jill Cox, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ATT1..txt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ordering special stain kits and control slides
I also use Poly Scientific and Newcomer with great success. But occasionally I use American Mastertech when Newcomer and PS don't have what I need. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Wednesday, August 17, 2011 10:46 AM Cc: Histonet@Lists. Edu Subject: Re: [Histonet] Ordering special stain kits and control slides Another vote for PolyScientific RD but Newcomer is also good and they also control slides. Andi Grantham On Aug 17, 2011, at 7:21 AM, Rathborne, Toni wrote: We use Poly Scientific www.PolyRnD.com Great support from their technical staff, timely deliveries, good quality. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jill Cox Sent: Wednesday, August 17, 2011 10:16 AM To: Histonet@Lists. Edu Subject: [Histonet] Ordering special stain kits and control slides Hi Histonetters, I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa. I do know the various vendors but wanted your input on who you use and why if possible. I also need to order HP control slides, same question.. Thanks in advance!! Jill Cox, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ATT1..txt ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Frozen Tissue Protocol
Hi everyone, Yesterday I asked for help with modifying my protocols to stain HSVI, HSVII, and Zoster. I appreciate everyone's help. Now I am back for more info. I deleted the depar. and cell conditioning steps and I got specific staining with little background. There was just one problem.the pathologist says that the leukocytes are reacting with the alk. phos on all of the slides. Does anyone know if a blocking step would help in this case? Just a reminder - I am using the Ventana Ultra platform with Ultraview Red Detection. Thanks again Histoland. You never let me down! J Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ordering special stain kits and control slides
Ditto for Poly Scientific. We are a research pathology lab and have used Poly Scientific exclusively for years. Their reagents are of excellent quality, their tech support great and turnaround from ordering to delivery is timely. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jill Cox Sent: Wednesday, August 17, 2011 10:16 AM To: Histonet@Lists. Edu Subject: [Histonet] Ordering special stain kits and control slides Hi Histonetters, I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa. I do know the various vendors but wanted your input on who you use and why if possible. I also need to order HP control slides, same question.. Thanks in advance!! Jill Cox, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] VALIDATION
Thanks for all of those who replied I have enough samples and I hope I forwarded them to all wanted a sample!! Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Creative Waste solutions
If I'm not mistaken You can find Rex's unit through Creative Waste Solutions (1-888-795-8300), Mercedes Medical, or American Master Tech Scientific. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Frozen Tissue Protocol
Paula, Where would I insert this step in my protocol if I am staining on the Ventana Ultra? Can I do it by hand after the slides are finished? Thanks for your help. Sheila From: Paula Pierce [mailto:cont...@excaliburpathology.com] Sent: Wednesday, August 17, 2011 11:14 AM To: Sheila Fonner Subject: Re: [Histonet] Frozen Tissue Protocol You are seeing endogenous phosphatase, not endogenous peroxidase. Use 10% acetic acid for 1-2 minutes. Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 631 N Broadway Moore, OK 73160 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com http://www.excaliburpathology.com/ _ From: Sheila Fonner sfon...@labpath.com To: histonet@lists.utsouthwestern.edu Sent: Wed, August 17, 2011 9:45:18 AM Subject: [Histonet] Frozen Tissue Protocol Hi everyone, Yesterday I asked for help with modifying my protocols to stain HSVI, HSVII, and Zoster. I appreciate everyone's help. Now I am back for more info. I deleted the depar. and cell conditioning steps and I got specific staining with little background. There was just one problem.the pathologist says that the leukocytes are reacting with the alk. phos on all of the slides. Does anyone know if a blocking step would help in this case? Just a reminder - I am using the Ventana Ultra platform with Ultraview Red Detection. Thanks again Histoland. You never let me down! J Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Opinions on Digital Pathology
Hello, I am interested in getting in touch with some pathology professionals to get their opinions on the current and future uses of digital pathology. Those interested please email me at: tngu...@kaiserassociates.com with your contact information. The conversation will last about 15 minutes and will cover current developments and current opinions on key attributes of the systems. Thanks! Tieuvi Nguyen / Kaiser Associates, Inc. M +1 202 506 0775 / tngu...@kaiserassociates.com 1201 Connecticut Ave NW, 12th Floor / Washington, DC 20036 USA www.kaiserassociates.com___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Opinions on Digital Pathology
There is lots of information regarding digital pathology on the DPA website - Digital Pathology Association - http://digitalpathologyassociation.org/ and there are groups in Linked In too. http://www.linkedin.com/groups?gid=163106trk=myg_ugrp_ovr http://www.linkedin.com/groups?gid=3362959trk=myg_ugrp_ovr You can contact me if you want to discuss Digital Pathology Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tieuvi Nguyen Sent: Wednesday, August 17, 2011 11:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Opinions on Digital Pathology Hello, I am interested in getting in touch with some pathology professionals to get their opinions on the current and future uses of digital pathology. Those interested please email me at: tngu...@kaiserassociates.com with your contact information. The conversation will last about 15 minutes and will cover current developments and current opinions on key attributes of the systems. Thanks! Tieuvi Nguyen / Kaiser Associates, Inc. M +1 202 506 0775 / tngu...@kaiserassociates.com 1201 Connecticut Ave NW, 12th Floor / Washington, DC 20036 USA www.kaiserassociates.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] pathology tracking systems
Hi, I was wondering if anyone is currently using or has had any experience with tracking systems for histology/gross room/pathology (such as Vantage from Ventana, or Lab Lion) and what your thoughts are on them? Thanks, Lisa V. Histology Manager Children's Memorial Hospital Chg., IL 773-868-8949 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Details about Masson Trichrome Stain !
Wang Lian MD, Medical School of Nanjing University in China, asks whether Biebrich Scarlet is Acid Red 66 or Acid Red 73. According to R.D. Lillie (both Histologic Technic 3rd ed. and Conn's Biological Stains 9th ed) states that the 2nd edition of the Colour Index (a rare book I have never seen a copy of) refers to Biebrich scarlet as Acid Red 66. Bob Richmond Samurai Pathologist Knoxville, Tennessee ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] melanin counterstaining
Good morning, I have heavily pigmented melanocytic cells and trying to differentiate between melanin pigment and chromogen (DAB) by using Azure B. After IHC with DAB I incubate slides for 30-45 min in the working solution of: * 4ml of a 25mg/ml aqueous solution of Azure B * 3.4ml of 0.1M acetic acid * 600µl of 0.1M sodium acetate * 27ml of DI water * 5ml acetone Then water wash and hematoxylin counter-stain. By the end all melanin supposed to turn blue-green, but I still see some remained brown melanin. My question is: I would like to increase green color, so the concentration of what reagent I have to increase? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: melanin counterstaining
You can try a simple Schmorl's stain - which stains melanin. You can find it in a Sheehan/Shrapchek Steven K. Olsen Chief Operating Officer, Director of Anatomic Pathology Molecular Pathology Laboratory Network, Inc. 250 E. Broadway, Maryville, TN 37804 P 865.380.9746 ext.187 C 865.978.0321 F 865.380.9191 http://www.MPLNET.com/ http://www.Geneuity.com/ Confidentiality Notice The information in this communication is confidential and intended for the exclusive use of the designated addressee. If you are not the addressee, any disclosure, reproduction or distribution of this communication and/or any attachments is strictly prohibited. If you are a covered entity and entitled to the information please notify us if there are exposures to the information contained in this communication as required by HIPAA and HITEC. If you received this communication in error, please notify the sender immediately. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Wednesday, August 17, 2011 2:51 PM To: histonet@lists.utsouthwestern.edu; histonet-requ...@lists.utsouthwestern.edu Subject: [Histonet] melanin counterstaining Good morning, I have heavily pigmented melanocytic cells and trying to differentiate between melanin pigment and chromogen (DAB) by using Azure B. After IHC with DAB I incubate slides for 30-45 min in the working solution of: * 4ml of a 25mg/ml aqueous solution of Azure B * 3.4ml of 0.1M acetic acid * 600µl of 0.1M sodium acetate * 27ml of DI water * 5ml acetone Then water wash and hematoxylin counter-stain. By the end all melanin supposed to turn blue-green, but I still see some remained brown melanin. My question is: I would like to increase green color, so the concentration of what reagent I have to increase? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: melanin counterstaining
I am not sure what others do, but I do not run my Azure B slides through alcohol because I find the alcohol diminishes the intensity of the green. I air dry, clear, then coverslip. Diane Craft Pathology Department Animal Medical Center 510 East 62nd St New York NY 10065-8314 212-329-8675 (phone) 212-759-5878 (fax) Steve Olsen sol...@mplnet.com Sent by: histonet-boun...@lists.utsouthwestern.edu 08/17/2011 02:56 PM To Margaryan, Naira nmargar...@childrensmemorial.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu cc Subject [Histonet] RE: melanin counterstaining You can try a simple Schmorl's stain - which stains melanin. You can find it in a Sheehan/Shrapchek Steven K. Olsen Chief Operating Officer, Director of Anatomic Pathology Molecular Pathology Laboratory Network, Inc. 250 E. Broadway, Maryville, TN 37804 P 865.380.9746 ext.187 C 865.978.0321 F 865.380.9191 http://www.MPLNET.com/ http://www.Geneuity.com/ Confidentiality Notice The information in this communication is confidential and intended for the exclusive use of the designated addressee. If you are not the addressee, any disclosure, reproduction or distribution of this communication and/or any attachments is strictly prohibited. If you are a covered entity and entitled to the information please notify us if there are exposures to the information contained in this communication as required by HIPAA and HITEC. If you received this communication in error, please notify the sender immediately. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Wednesday, August 17, 2011 2:51 PM To: histonet@lists.utsouthwestern.edu; histonet-requ...@lists.utsouthwestern.edu Subject: [Histonet] melanin counterstaining Good morning, I have heavily pigmented melanocytic cells and trying to differentiate between melanin pigment and chromogen (DAB) by using Azure B. After IHC with DAB I incubate slides for 30-45 min in the working solution of: * 4ml of a 25mg/ml aqueous solution of Azure B * 3.4ml of 0.1M acetic acid * 600µl of 0.1M sodium acetate * 27ml of DI water * 5ml acetone Then water wash and hematoxylin counter-stain. By the end all melanin supposed to turn blue-green, but I still see some remained brown melanin. My question is: I would like to increase green color, so the concentration of what reagent I have to increase? Thanks in advance, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ordering special stain kits and control slides
I also had great results with American Mastertech's stain kits and agree that their customer service is great. I've ordered HP control slides from Mercedes Medical and Newcomer Supply and have had good results with both of those as well. -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 On Wed, Aug 17, 2011 at 7:15 AM, Jill Cox jco...@yahoo.com wrote: Hi Histonetters, I need to order special stain kits for PAS, Alcian Blue and Wright Giemsa. I do know the various vendors but wanted your input on who you use and why if possible. I also need to order HP control slides, same question.. Thanks in advance!! Jill Cox, HT ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] T-bet antibody
Dear Colleagues, Has somebody results with an anti mouse T-bet antibody, who is working fine on paraffin sections and formaline fixed. Best Regards, Henk van de Kant ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
