[Histonet] Histology Technician Position in Santa Monica, Calif.
New GI Lab in Santa Monica California seeks registered HT. Experienced in all histology functions including some grossing experience. QA, QC experience preferred and must work well independently. For more detailed information please e-mail me at the address below. THANK YOU, PATTI RUBEN-NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonr...@verizon.net CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 909-841-9761. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] VIP PROGRAMMING
HI, EVERYONE QUESTION: WITH THE VIP SAKURA WHEN PROGRAMMING FOR A WKEND IS THE END DAY/TIME 3/00:00 OR 2/00:00 AND THEN FOR A 3 DAY WKEND (LIKE MEMORIAL DAY) IS THE END DAY/TIME 4/00:00 OR 3/00:00. JUST WANT TO MAKE SURE. THANK YOU FOR ALL THE HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] VIP PROGRAMMING
Kristy, 00 is today friday 01 tomorrow sat 02 the day after tommorrowsun 03 three days from today mon 04 four days from todaY TUES so for a regular weekend you would want the end time to be on day 03 at what ever time you set. this will end monday morning For a three days weekend or holiday you would want your end time to be 04 at what ever time you set. this will end tuesday morning. Hope this helps, Nicole Tatum, HT ASCP HI, EVERYONE QUESTION: WITH THE VIP SAKURA WHEN PROGRAMMING FOR A WKEND IS THE END DAY/TIME 3/00:00 OR 2/00:00 AND THEN FOR A 3 DAY WKEND (LIKE MEMORIAL DAY) IS THE END DAY/TIME 4/00:00 OR 3/00:00. JUST WANT TO MAKE SURE. THANK YOU FOR ALL THE HELP. KRISTY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Glass Slides
I put mine in the sharps box, unless I have alot then I put in cardboard hazard box. Nicole Tatum, HT ASCP I have a lot of slides that I have precut for stains. These slides are only labeled with the accession numbers and were not used as any part of the patient diagnosis. I was wondering how other labs were disposing of these slides. Thanks, Travis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology PI
Happy Friday Histonetters, Hope everyone has a great weekend while I pull some thoughts together about histology PI studies. I am looking for any help possible from the histonet that can help me with a Performance Improvement Plan. I am now required to come up with two studies a year and have no idea what to do or how to go about doing it. So I am seeking much needed help in the PI area for histology. Thanks in advance for your help. Amy Self Georgetown Hospital System Dept. of Pathology NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histology PI
We are required to do 3, a pre-analytic, analytic, and post-analytic. So from that you can do things like specimen id (whether received from the OR that way, or the cassettes mislabeled during grossing, or the slides mislabeled). You can compare % of cytology/histology cases correlated, TAT with regard to routine cases or autopsy, FS TAT, repeated stains, the list is endless. I would begin where ever you have some issues you would like to improve on, or if you are a CAP accredited facility use their standards. If you are able to retrieve the information for the last 6 months, I would use that as a baseline. Look for small changes, and work to gradually improve. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Friday, September 23, 2011 11:00 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Histology PI Happy Friday Histonetters, Hope everyone has a great weekend while I pull some thoughts together about histology PI studies. I am looking for any help possible from the histonet that can help me with a Performance Improvement Plan. I am now required to come up with two studies a year and have no idea what to do or how to go about doing it. So I am seeking much needed help in the PI area for histology. Thanks in advance for your help. Amy Self Georgetown Hospital System Dept. of Pathology NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Milestone Logos
I was able to see the Logos in Italy and it is a very nice piece of equipment, very robust and with many safety features. For its price it is a much better investment than the Xpress 50 and produces the same quality of processing with the same mixed technology (microwave and convection). For those used to manually advance the tissues between two stations, it will be the perfect instrument to deliver a throughput technology in small batches. René J. --- On Thu, 9/22/11, Shelly Coker sccrsh...@yahoo.com wrote: From: Shelly Coker sccrsh...@yahoo.com Subject: [Histonet] Milestone Logos To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Thursday, September 22, 2011, 9:50 PM I would love to hear some feedback from individuals that went to NSH and saw the new Milestone Logos. We are considering adding a new automated processor to our lab due to an increase in volume over the past few years and this is one of the options. Background info: we already use a microwave processor with great success, we just need something a bit more automated. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histology PI
Whatever study you are going to do for your PI program has to do with some mistake or defect you had previously detected. Lets say that one of your FS took over the 20 minutes required by CAP. You should have taken action in finding out what happened. Then you should have followed up with such action. Now you could look back to your numbers and determine if after you took the action to correct the problem, that has happened again or not. This could be one. On the same like of thought you could do other studies on some other problems detected in your lab that caused a PI action. René J. --- On Fri, 9/23/11, Amy Self as...@georgetownhospitalsystem.org wrote: From: Amy Self as...@georgetownhospitalsystem.org Subject: [Histonet] Histology PI To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Date: Friday, September 23, 2011, 11:00 AM Happy Friday Histonetters, Hope everyone has a great weekend while I pull some thoughts together about histology PI studies. I am looking for any help possible from the histonet that can help me with a Performance Improvement Plan. I am now required to come up with two studies a year and have no idea what to do or how to go about doing it. So I am seeking much needed help in the PI area for histology. Thanks in advance for your help. Amy Self Georgetown Hospital System Dept. of Pathology NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Thermo Shandon Histobath
Matt Mincer at Tech One Biomedical Services In Oak Park IL notes: We are releasing a version of the Histobath in the next few months. In fact, we had the beta at out booth at NSH and are implementing several of the suggestions we received there. I hope that one of those suggestions was to try to get people to use a non-flammable fluorcarbon instead of dangerous methylbutane and acetone. I've posted several notes about - archived on Histosearch, if you haven't read them already. I'd appreciate being kept up to date about this new product. Bob Richmond rsrichm...@gmail.com Samurai Pathologist Knoxville TN *** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?
Hello, I am a newbie to this type of work. We have flash frozen tissue in OCT (Optimal Cutting Temperature) compound for some unique experiments that we need to carry out. We want to use a AO-860 sliding microtome to cut large slabs of OCT embedded tissue. Does anyone have any advice on how this would work reproducibly and reliably on the freezing stage. Best, Francis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Thermo Shandon Histobath
Thanks to Matt Mincer and Terri Bishop for sending me information about Histochill. I'm glad they're promoting the 3M fluorocarbon solvent as an alternative to methylbutane and acetone. You can download the PDF with I think the same information - Google histochill. Anybody know what this item costs? Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Myeloblasts
Can anyone tell me what is a good Ab to use for staining myeloblasts in bone marrow specimens? I would appreciate any input. Thanks Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?
Hi Francis, I don't know how thick your sections will be, but in general the AO-860 (fantastic microtome by the way) is not designed to cut fresh tissue. Certainly the relatively thick sections brain sections (50-200 micron) won't stand up very well in my experience. How thick are you trying to go and what's the tissue. It might make a difference. Caroline On Sep 23, 2011, at 1:05 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Message: 4 Date: Fri, 23 Sep 2011 12:03:03 -0400 From: Francis OBrien francisobrien2...@gmail.com Subject: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue? To: histonet@lists.utsouthwestern.edu Message-ID: cakmfspfpqdo1ebmg_bcfskvajzplcevytdkq2hvp_dzkkr8...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hello, I am a newbie to this type of work. We have flash frozen tissue in OCT (Optimal Cutting Temperature) compound for some unique experiments that we need to carry out. We want to use a AO-860 sliding microtome to cut large slabs of OCT embedded tissue. Does anyone have any advice on how this would work reproducibly and reliably on the freezing stage. Best, Francis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?
Unless you have means to maintain the frozen tissue frozen, you will not be able to do it because the tissue will thaw and sectioning will be impossible. René J. --- On Fri, 9/23/11, Francis OBrien francisobrien2...@gmail.com wrote: From: Francis OBrien francisobrien2...@gmail.com Subject: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue? To: histonet@lists.utsouthwestern.edu Date: Friday, September 23, 2011, 12:03 PM Hello, I am a newbie to this type of work. We have flash frozen tissue in OCT (Optimal Cutting Temperature) compound for some unique experiments that we need to carry out. We want to use a AO-860 sliding microtome to cut large slabs of OCT embedded tissue. Does anyone have any advice on how this would work reproducibly and reliably on the freezing stage. Best, Francis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?
I would be using the freezing stage with dry ice. On Fri, Sep 23, 2011 at 3:33 PM, Rene J Buesa rjbu...@yahoo.com wrote: Unless you have means to maintain the frozen tissue frozen, you will not be able to do it because the tissue will thaw and sectioning will be impossible. René J. --- On *Fri, 9/23/11, Francis OBrien francisobrien2...@gmail.com* wrote: From: Francis OBrien francisobrien2...@gmail.com Subject: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue? To: histonet@lists.utsouthwestern.edu Date: Friday, September 23, 2011, 12:03 PM Hello, I am a newbie to this type of work. We have flash frozen tissue in OCT (Optimal Cutting Temperature) compound for some unique experiments that we need to carry out. We want to use a AO-860 sliding microtome to cut large slabs of OCT embedded tissue. Does anyone have any advice on how this would work reproducibly and reliably on the freezing stage. Best, Francis ___ Histonet mailing list Histonet@lists.utsouthwestern.eduhttp://us.mc657.mail.yahoo.com/mc/compose?to=Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Regards, Francis Research graduate, New York School of Medicine New York ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] STAIN PRECIPITATE
Hi Histonetters I have the Ventana xt, H pylori from cell marque and my pathologists says there is too much stain precipitate and was wondering if I could do something about it my protocol is mild cc1 standard ABY H pylori 32 min ultra wash and counterstains Any help is appreciated Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] STAIN PRECIPITATE
We've been seeing high background staining on abt 10% of our HPs as well. We use Cellmarques predilute on the XT as well. Been happening for a couple months. Sara Baldwin/mhhcc.org sbald...@mhhcc.org 9/23/2011 3:57 PM Hi Histonetters I have the Ventana xt, H pylori from cell marque and my pathologists says there is too much stain precipitate and was wondering if I could do something about it my protocol is mild cc1 standard ABY H pylori 32 min ultra wash and counterstains Any help is appreciated Thanks Pathology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 Confidential information, Authorized use only. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Plastic embedding in dallas area
Does anybody in Dallas,TX area are working on plastic embedding that are charging them. Please let me know because I have a student from UTA who are working on fiber optic on nerve tissue that wants to do plastic embedding. Our facility does not work on outside samples unless we are in collaboration. Thank you for your help. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue?
Hi, Got a walk in freezer? Really, cutting large slabs of OCT embedded material is just not possible on a sliding microtome unless you keep that microtome in a -20 freezer. You could cut small blocks on it by mounting the OCT on a large chuck and surrounding it with dry ice. This will really only work with small blocks though, since the center of the larger slabs will not be in direct contact with the ice and will melt. You would be better off using paraffin embedded material. Find a good priest because I anticipate you'll be doing a lot of cursing. Good luck, Amos On Fri, Sep 23, 2011 at 1:00 PM, histonet-requ...@lists.utsouthwestern.eduwrote: Message: 4 Date: Fri, 23 Sep 2011 12:03:03 -0400 From: Francis OBrien francisobrien2...@gmail.com Subject: [Histonet] AO-860 Sliding Microtome + OCT Embedded Tissue? To: histonet@lists.utsouthwestern.edu Message-ID: cakmfspfpqdo1ebmg_bcfskvajzplcevytdkq2hvp_dzkkr8...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hello, I am a newbie to this type of work. We have flash frozen tissue in OCT (Optimal Cutting Temperature) compound for some unique experiments that we need to carry out. We want to use a AO-860 sliding microtome to cut large slabs of OCT embedded tissue. Does anyone have any advice on how this would work reproducibly and reliably on the freezing stage. Best, Francis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Predicting antibody IHC activity
Hi All, I am wondering if anyone knows of any particular publications or studies that have examined manners in which one can predict whether an antibody in a large panel may work in IHC. Let's say you are faced with 50-100 Abs and you need to determine which works in IHC, but all you know is binding affinity, activity in western, ELISA etc ... what are the best factors about an antibody that correlate to or predict FFPE IHC activity? If anyone has done these studies or has an opinion I would appreciate it. Best, Andrea Hooper ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
