RE: [Histonet] P 16
Hi Phyllis, We are doing a double stain (Mib-1 - P16) on cervix biopsies in which the P16 antibody is coloured with AP. It works fine, our pathologists are quite fond of it. Almost every day we have a few slides. Willem Hoekert OLVG, Amsterdam The Netherlands Van: histonet-boun...@lists.utsouthwestern.edu namens Phyllis Thaxton Verzonden: wo 28-9-2011 21:14 Aan: Histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] P 16 Has anyone ever used P16 with AP Red detection on the Ventana Benchmark? Phyllis Jordan HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?
The argument against using toxic, yet recyclable xylene in favor of a more expensive, less efficacious xylene-substitute like Slide Bright in larger labs is not compelling. Aside from low volatility of Slide-Bright, what is gained in using a more expensive substitute whose toxicities are not well-known? The chemical composition differs, yet most MSDS warning are the same. The advantage for low volatility can be an advantage for small hole in the wall lab settings, like an office Mohs lab or frozen section lab in the OR suite where ventilation may be an issue. Unlike xylene where 75% recycling yield is norm , Slide-Bright can be recycled- how well, I don't know. I've requested specifics from B/R for a protocol and will forward that info later. However, if Slide-Bright is disposed like xylene it carries the same disposal cost. The company indicates Slide-Bright is a flammable aliphatic hydrocarbon which laden with paraffin may be disposed of down the drain with copious amounts of water, yet it is the lab directors job to ensure all local state and federal guidelines are followed. Aren't you defeating part of your purpose in working toward a safer lab and greener environment by dumping aliphatic hydrocarbons into your ground water? Steve Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 Slide-Bright MSDS follows: Revision Date: 6/1/2006 MATERIAL SAFETY DATA SHEET Conforms to 93/112/EC and ISO 11014-1 1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION PRODUCT NAME:OptiClear E PRODUCT NUMBER: OE-104 CHEMICAL NAMES/ DESCRIPTION: Aliphatic Hydrocarbon MANUFACTURER: National Diagnostics, Inc. 305 Patton Drive Atlanta, GA 30336 TELEPHONE NUMBER: (800) 526-3867 (404) 699-2121 EMERGENCY NUMBER: CHEMTREC (800) 424-9300 2. COMPOSITION / INFORMATION ON INGREDIENTS Component% Comp CAS #EINECS # TLV (units) Aliphatic Hydrocarbons EEC LABEL SYMBOL AND CLASSIFICATION R: 11-38 100 400 ppm Highly flammable. Irritating to skin. S: (2-) 16-23-24-62 Keep out of the reach of children. Keep away from sources of ignition - No Smoking. Do not breathe fumes. Avoid contact with the skin. If swallowed, do not induce vomiting: Seek medical advice immediately and show this container or label. 3. HAZARDS IDENTIFICATION APPEARANCE AND ODOR: Clear, colorless liquid EMERGENCY OVERVIEW - IMMEDIATE HAZARD HIGHLY FLAMMABLE. PRODUCT IS SLIGHTLY IRRITATING TO EYES (NO INJURY). HIGH VAPOR MAY CAUSE RESPIRATORY TRACT IRRITATION, HEADACHE, DIZZINESS, ANESTHESIA, DROWSINESS, UNCONSCIOUSNESS, OR DEATH. INGESTION: MINIMAL TOXICITY. ASPIRATION MAY LEAD TO PULMONARY INJURY AND DEATH. EMERGENCY OVERVIEW - CHRONIC HAZARD W ARNING NO CHRONIC HAZARDS SUSPECTED. POTENTIAL HEALTH EFFECTS INHALATION High vapor/aerosol concentrations (greater than approximately 1000 ppm) are irritating to the eyes and the respiratory tract, may cause headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other central nervous system effects, including death. INGESTION Minimal toxicity by ingestion, though small amounts of this product aspirated into the respiratory system durin ingestion or vomiting may cause mild to severe pulmonary injury, possibly progressing to death. SKIN Low order or toxicity. Frequent or prolonged contact may irritate and cause dermatitis. Skin contact may aggravate an existing dermatitis condition. EYES Slightly irritating but does not injure eye tissue. SIGNS AND SYMPTOMS 0F OVEREXPOSURE INHALATION Page 1 OptiClear E Headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other central nervous system effects, including death. INGESTION Minimal toxicity by ingestion. SKIN Dermatitis may occur with frequent or prolonged contact. EYES Product is only slightly irritating to eye tissue, non injurious. CARCINOGENICITY Not listed by NTP, IARC, or OSHA. MUTAGENICITY No information available. REPRODUCTIVE TOXICITY No information available. TERATOGENIC EFFECTS No information available. ROUTES OF ENTRY Inhalation or by skin contact. TARGET ORGAN STATEMENT No information available. 4. FIRST AID MEASURES INHALATION: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physicia INGESTION: Do not induce vomiting. If swallowed and the person is conscious, immediately give large amounts of water. Get medical attention. SKIN: Immediately flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Get medical attention. Wash clothing before reuse. Thoroughly clean shoes before reuse. EYES: Immediately flush eyes with plenty of water for at least fifteen minutes, lifting lower and upper eyelids occasionally. Get medical attention
RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?
Steve, I totally agree. NOTHING but water should go down the drain. Ruth N.I.H. -Original Message- From: Steve McClain [mailto:ste...@mcclainlab.com] Sent: Friday, September 30, 2011 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain? The argument against using toxic, yet recyclable xylene in favor of a more expensive, less efficacious xylene-substitute like Slide Bright in larger labs is not compelling. Aside from low volatility of Slide-Bright, what is gained in using a more expensive substitute whose toxicities are not well-known? The chemical composition differs, yet most MSDS warning are the same. The advantage for low volatility can be an advantage for small hole in the wall lab settings, like an office Mohs lab or frozen section lab in the OR suite where ventilation may be an issue. Unlike xylene where 75% recycling yield is norm , Slide-Bright can be recycled- how well, I don't know. I've requested specifics from B/R for a protocol and will forward that info later. However, if Slide-Bright is disposed like xylene it carries the same disposal cost. The company indicates Slide-Bright is a flammable aliphatic hydrocarbon which laden with paraffin may be disposed of down the drain with copious amounts of water, yet it is the lab directors job to ensure all local state and federal guidelines are followed. Aren't you defeating part of your purpose in working toward a safer lab and greener environment by dumping aliphatic hydrocarbons into your ground water? Steve Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 Slide-Bright MSDS follows: Revision Date: 6/1/2006 MATERIAL SAFETY DATA SHEET Conforms to 93/112/EC and ISO 11014-1 1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION PRODUCT NAME:OptiClear E PRODUCT NUMBER: OE-104 CHEMICAL NAMES/ DESCRIPTION: Aliphatic Hydrocarbon MANUFACTURER: National Diagnostics, Inc. 305 Patton Drive Atlanta, GA 30336 TELEPHONE NUMBER: (800) 526-3867 (404) 699-2121 EMERGENCY NUMBER: CHEMTREC (800) 424-9300 2. COMPOSITION / INFORMATION ON INGREDIENTS Component% Comp CAS #EINECS # TLV (units) Aliphatic Hydrocarbons EEC LABEL SYMBOL AND CLASSIFICATION R: 11-38 100 400 ppm Highly flammable. Irritating to skin. S: (2-) 16-23-24-62 Keep out of the reach of children. Keep away from sources of ignition - No Smoking. Do not breathe fumes. Avoid contact with the skin. If swallowed, do not induce vomiting: Seek medical advice immediately and show this container or label. 3. HAZARDS IDENTIFICATION APPEARANCE AND ODOR: Clear, colorless liquid EMERGENCY OVERVIEW - IMMEDIATE HAZARD HIGHLY FLAMMABLE. PRODUCT IS SLIGHTLY IRRITATING TO EYES (NO INJURY). HIGH VAPOR MAY CAUSE RESPIRATORY TRACT IRRITATION, HEADACHE, DIZZINESS, ANESTHESIA, DROWSINESS, UNCONSCIOUSNESS, OR DEATH. INGESTION: MINIMAL TOXICITY. ASPIRATION MAY LEAD TO PULMONARY INJURY AND DEATH. EMERGENCY OVERVIEW - CHRONIC HAZARD W ARNING NO CHRONIC HAZARDS SUSPECTED. POTENTIAL HEALTH EFFECTS INHALATION High vapor/aerosol concentrations (greater than approximately 1000 ppm) are irritating to the eyes and the respiratory tract, may cause headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other central nervous system effects, including death. INGESTION Minimal toxicity by ingestion, though small amounts of this product aspirated into the respiratory system durin ingestion or vomiting may cause mild to severe pulmonary injury, possibly progressing to death. SKIN Low order or toxicity. Frequent or prolonged contact may irritate and cause dermatitis. Skin contact may aggravate an existing dermatitis condition. EYES Slightly irritating but does not injure eye tissue. SIGNS AND SYMPTOMS 0F OVEREXPOSURE INHALATION Page 1 OptiClear E Headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other central nervous system effects, including death. INGESTION Minimal toxicity by ingestion. SKIN Dermatitis may occur with frequent or prolonged contact. EYES Product is only slightly irritating to eye tissue, non injurious. CARCINOGENICITY Not listed by NTP, IARC, or OSHA. MUTAGENICITY No information available. REPRODUCTIVE TOXICITY No information available. TERATOGENIC EFFECTS No information available. ROUTES OF ENTRY Inhalation or by skin contact. TARGET ORGAN STATEMENT No information available. 4. FIRST AID MEASURES INHALATION: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physicia INGESTION: Do not induce vomiting. If swallowed and the person is conscious, immediately give large amounts of water. Get medical attention.
RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?
What about Alcohol formalin from the processor? The diluted alcohol (100, 95, and 75%) and the alcohol from the cleaning cycle? Do you not pour that down the drain with water? And, what about the HE stainer...do you not pour the bluing, hematoxylin, eosin and acid alcohol down the drain? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth A (NIH/NIDCR) [E] Sent: Friday, September 30, 2011 9:06 AM To: Steve McClain; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain? Steve, I totally agree. NOTHING but water should go down the drain. Ruth N.I.H. -Original Message- From: Steve McClain [mailto:ste...@mcclainlab.com] Sent: Friday, September 30, 2011 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain? The argument against using toxic, yet recyclable xylene in favor of a more expensive, less efficacious xylene-substitute like Slide Bright in larger labs is not compelling. Aside from low volatility of Slide-Bright, what is gained in using a more expensive substitute whose toxicities are not well-known? The chemical composition differs, yet most MSDS warning are the same. The advantage for low volatility can be an advantage for small hole in the wall lab settings, like an office Mohs lab or frozen section lab in the OR suite where ventilation may be an issue. Unlike xylene where 75% recycling yield is norm , Slide-Bright can be recycled- how well, I don't know. I've requested specifics from B/R for a protocol and will forward that info later. However, if Slide-Bright is disposed like xylene it carries the same disposal cost. The company indicates Slide-Bright is a flammable aliphatic hydrocarbon which laden with paraffin may be disposed of down the drain with copious amounts of water, yet it is the lab directors job to ensure all local state and federal guidelines are followed. Aren't you defeating part of your purpose in working toward a safer lab and greener environment by dumping aliphatic hydrocarbons into your ground water? Steve Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 Slide-Bright MSDS follows: Revision Date: 6/1/2006 MATERIAL SAFETY DATA SHEET Conforms to 93/112/EC and ISO 11014-1 1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION PRODUCT NAME:OptiClear E PRODUCT NUMBER: OE-104 CHEMICAL NAMES/ DESCRIPTION: Aliphatic Hydrocarbon MANUFACTURER: National Diagnostics, Inc. 305 Patton Drive Atlanta, GA 30336 TELEPHONE NUMBER: (800) 526-3867 (404) 699-2121 EMERGENCY NUMBER: CHEMTREC (800) 424-9300 2. COMPOSITION / INFORMATION ON INGREDIENTS Component% Comp CAS #EINECS # TLV (units) Aliphatic Hydrocarbons EEC LABEL SYMBOL AND CLASSIFICATION R: 11-38 100 400 ppm Highly flammable. Irritating to skin. S: (2-) 16-23-24-62 Keep out of the reach of children. Keep away from sources of ignition - No Smoking. Do not breathe fumes. Avoid contact with the skin. If swallowed, do not induce vomiting: Seek medical advice immediately and show this container or label. 3. HAZARDS IDENTIFICATION APPEARANCE AND ODOR: Clear, colorless liquid EMERGENCY OVERVIEW - IMMEDIATE HAZARD HIGHLY FLAMMABLE. PRODUCT IS SLIGHTLY IRRITATING TO EYES (NO INJURY). HIGH VAPOR MAY CAUSE RESPIRATORY TRACT IRRITATION, HEADACHE, DIZZINESS, ANESTHESIA, DROWSINESS, UNCONSCIOUSNESS, OR DEATH. INGESTION: MINIMAL TOXICITY. ASPIRATION MAY LEAD TO PULMONARY INJURY AND DEATH. EMERGENCY OVERVIEW - CHRONIC HAZARD W ARNING NO CHRONIC HAZARDS SUSPECTED. POTENTIAL HEALTH EFFECTS INHALATION High vapor/aerosol concentrations (greater than approximately 1000 ppm) are irritating to the eyes and the respiratory tract, may cause headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other central nervous system effects, including death. INGESTION Minimal toxicity by ingestion, though small amounts of this product aspirated into the respiratory system durin ingestion or vomiting may cause mild to severe pulmonary injury, possibly progressing to death. SKIN Low order or toxicity. Frequent or prolonged contact may irritate and cause dermatitis. Skin contact may aggravate an existing dermatitis condition. EYES Slightly irritating but does not injure eye tissue. SIGNS AND SYMPTOMS 0F OVEREXPOSURE INHALATION Page 1 OptiClear E Headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other central nervous system effects, including death. INGESTION Minimal toxicity by ingestion. SKIN Dermatitis may occur
RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?
Amber: Actually, no we do not dump the items you listed down the drain. They get recycled appropriately. Joe -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Friday, September 30, 2011 9:25 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain? What about Alcohol formalin from the processor? The diluted alcohol (100, 95, and 75%) and the alcohol from the cleaning cycle? Do you not pour that down the drain with water? And, what about the HE stainer...do you not pour the bluing, hematoxylin, eosin and acid alcohol down the drain? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth A (NIH/NIDCR) [E] Sent: Friday, September 30, 2011 9:06 AM To: Steve McClain; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain? Steve, I totally agree. NOTHING but water should go down the drain. Ruth N.I.H. -Original Message- From: Steve McClain [mailto:ste...@mcclainlab.com] Sent: Friday, September 30, 2011 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain? The argument against using toxic, yet recyclable xylene in favor of a more expensive, less efficacious xylene-substitute like Slide Bright in larger labs is not compelling. Aside from low volatility of Slide-Bright, what is gained in using a more expensive substitute whose toxicities are not well-known? The chemical composition differs, yet most MSDS warning are the same. The advantage for low volatility can be an advantage for small hole in the wall lab settings, like an office Mohs lab or frozen section lab in the OR suite where ventilation may be an issue. Unlike xylene where 75% recycling yield is norm , Slide-Bright can be recycled- how well, I don't know. I've requested specifics from B/R for a protocol and will forward that info later. However, if Slide-Bright is disposed like xylene it carries the same disposal cost. The company indicates Slide-Bright is a flammable aliphatic hydrocarbon which laden with paraffin may be disposed of down the drain with copious amounts of water, yet it is the lab directors job to ensure all local state and federal guidelines are followed. Aren't you defeating part of your purpose in working toward a safer lab and greener environment by dumping aliphatic hydrocarbons into your ground water? Steve Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 Slide-Bright MSDS follows: Revision Date: 6/1/2006 MATERIAL SAFETY DATA SHEET Conforms to 93/112/EC and ISO 11014-1 1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION PRODUCT NAME:OptiClear E PRODUCT NUMBER: OE-104 CHEMICAL NAMES/ DESCRIPTION: Aliphatic Hydrocarbon MANUFACTURER: National Diagnostics, Inc. 305 Patton Drive Atlanta, GA 30336 TELEPHONE NUMBER: (800) 526-3867 (404) 699-2121 EMERGENCY NUMBER: CHEMTREC (800) 424-9300 2. COMPOSITION / INFORMATION ON INGREDIENTS Component% Comp CAS #EINECS # TLV (units) Aliphatic Hydrocarbons EEC LABEL SYMBOL AND CLASSIFICATION R: 11-38 100 400 ppm Highly flammable. Irritating to skin. S: (2-) 16-23-24-62 Keep out of the reach of children. Keep away from sources of ignition - No Smoking. Do not breathe fumes. Avoid contact with the skin. If swallowed, do not induce vomiting: Seek medical advice immediately and show this container or label. 3. HAZARDS IDENTIFICATION APPEARANCE AND ODOR: Clear, colorless liquid EMERGENCY OVERVIEW - IMMEDIATE HAZARD HIGHLY FLAMMABLE. PRODUCT IS SLIGHTLY IRRITATING TO EYES (NO INJURY). HIGH VAPOR MAY CAUSE RESPIRATORY TRACT IRRITATION, HEADACHE, DIZZINESS, ANESTHESIA, DROWSINESS, UNCONSCIOUSNESS, OR DEATH. INGESTION: MINIMAL TOXICITY. ASPIRATION MAY LEAD TO PULMONARY INJURY AND DEATH. EMERGENCY OVERVIEW - CHRONIC HAZARD W ARNING NO CHRONIC HAZARDS SUSPECTED. POTENTIAL HEALTH EFFECTS INHALATION High vapor/aerosol concentrations (greater than approximately 1000 ppm) are irritating to the eyes and the respiratory tract, may cause headaches, dizziness, anesthesia, drowsiness, unconsciousness, and other central nervous system effects, including death. INGESTION Minimal toxicity by ingestion, though small amounts of this product aspirated into the respiratory system durin ingestion or vomiting may cause mild to severe pulmonary injury, possibly progressing to death. SKIN Low order
[Histonet] Alignment tool
Who sells the microtome alignment tool that clamps onto the base of the microtome and also into the cassette holder to assure that all microtomes in the lab are in the same cutting plane? Do the bubble ones work as well? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain?
We collect EVERYTHING for recycling or disposal by our site-wide contractor http://www.cleanharbors.com/ Only environmentally approved hand soap and glassware cleansers are allowed down the drain, along with water. Brett M. Connolly, Ph.D. Molecular Imaging Team Leader Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Galbraith, Joe Sent: Friday, September 30, 2011 10:35 AM To: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain? Amber: Actually, no we do not dump the items you listed down the drain. They get recycled appropriately. Joe -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Friday, September 30, 2011 9:25 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain? What about Alcohol formalin from the processor? The diluted alcohol (100, 95, and 75%) and the alcohol from the cleaning cycle? Do you not pour that down the drain with water? And, what about the HE stainer...do you not pour the bluing, hematoxylin, eosin and acid alcohol down the drain? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yaskovich, Ruth A (NIH/NIDCR) [E] Sent: Friday, September 30, 2011 9:06 AM To: Steve McClain; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain? Steve, I totally agree. NOTHING but water should go down the drain. Ruth N.I.H. -Original Message- From: Steve McClain [mailto:ste...@mcclainlab.com] Sent: Friday, September 30, 2011 9:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 94, Issue 38 Xylene safe disposal vs. aliphatic hydrocarbons e.g., Slide Bright, down the drain? The argument against using toxic, yet recyclable xylene in favor of a more expensive, less efficacious xylene-substitute like Slide Bright in larger labs is not compelling. Aside from low volatility of Slide-Bright, what is gained in using a more expensive substitute whose toxicities are not well-known? The chemical composition differs, yet most MSDS warning are the same. The advantage for low volatility can be an advantage for small hole in the wall lab settings, like an office Mohs lab or frozen section lab in the OR suite where ventilation may be an issue. Unlike xylene where 75% recycling yield is norm , Slide-Bright can be recycled- how well, I don't know. I've requested specifics from B/R for a protocol and will forward that info later. However, if Slide-Bright is disposed like xylene it carries the same disposal cost. The company indicates Slide-Bright is a flammable aliphatic hydrocarbon which laden with paraffin may be disposed of down the drain with copious amounts of water, yet it is the lab directors job to ensure all local state and federal guidelines are followed. Aren't you defeating part of your purpose in working toward a safer lab and greener environment by dumping aliphatic hydrocarbons into your ground water? Steve Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 Slide-Bright MSDS follows: Revision Date: 6/1/2006 MATERIAL SAFETY DATA SHEET Conforms to 93/112/EC and ISO 11014-1 1. CHEMICAL PRODUCT AND COMPANY IDENTIFICATION PRODUCT NAME:OptiClear E PRODUCT NUMBER: OE-104 CHEMICAL NAMES/ DESCRIPTION: Aliphatic Hydrocarbon MANUFACTURER: National Diagnostics, Inc. 305 Patton Drive Atlanta, GA 30336 TELEPHONE NUMBER: (800) 526-3867 (404) 699-2121 EMERGENCY NUMBER: CHEMTREC (800) 424-9300 2. COMPOSITION / INFORMATION ON INGREDIENTS Component% Comp CAS #EINECS # TLV (units) Aliphatic Hydrocarbons EEC LABEL SYMBOL AND CLASSIFICATION R: 11-38 100 400 ppm Highly flammable. Irritating to skin. S: (2-) 16-23-24-62 Keep out of the reach of children. Keep away from sources of ignition - No Smoking. Do not breathe fumes. Avoid contact with the skin. If swallowed, do not induce vomiting: Seek medical advice immediately and show this container or label. 3. HAZARDS IDENTIFICATION APPEARANCE AND ODOR: Clear, colorless liquid EMERGENCY OVERVIEW - IMMEDIATE HAZARD HIGHLY FLAMMABLE. PRODUCT IS SLIGHTLY IRRITATING TO EYES (NO INJURY). HIGH VAPOR MAY CAUSE RESPIRATORY TRACT
RE: [Histonet] Alignment tool
Newcomer sells one Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jsjurc...@comcast.net Sent: Friday, September 30, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alignment tool Who sells the microtome alignment tool that clamps onto the base of the microtome and also into the cassette holder to assure that all microtomes in the lab are in the same cutting plane? Do the bubble ones work as well? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: KP Markers!
If you would like to obtain KP Markers, they are now being carried by Sensor Health, inc. in Cambridge, Ontario, Canada. If you have any questions you can contact Adam Harris at 888-777-7080 or 519-241-2194. You can check out their website at www.sensorhealth.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Background in FFPE glandular tissue sections
Mesruh: The easiest method to address such is a problem is to apply a serum-free (e.g. casein-based) protein blocking agent, which can be obtained from a variety of vendors. This technique is particularly effective when the reagent is allowed to incubate with the specimen for 5 to 10 minutes at RT, and then, rather than rinsing it off the slide with buffer, it is drained off or blown off; that way, when the primary antibody solution is applied (immediately after this removal step), the primary has to 'work through' the residual blocking agent, resulting in the binding of only high-avidity/affinity antibodies to the target antigen. Good Luck, Joe Myers, M.S., CT(ASCP)QIHC -- Message: 3 Date: Thu, 29 Sep 2011 13:10:58 -0400 From: mesruh turkekul turke...@gmail.com Subject: [Histonet] Background in FFPE glandular tissue sections during IHC To: _histonet@lists.utsouthwestern.edu_ (mailto:histonet@lists.utsouthwestern.edu) Dear Histonetters, When I do IHC on FFPE tissue sections. Most of time there is background due to trapping of IgG/detection reagents probably in the connective tissue fibers, mucins or other secretions of the glandular tissue. Especially prostate and mammary gland. Any tips to get rid of that kind of background? Do you know any treatment to block mucins or connective tissue fibers from nonspecifically and electrostatically attracting IgGs and detection reagents? Regards, Mesruh Turkekul mskcc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Alignment tool
I actually just got a flyer for one of those from American Mastertech that looked pretty neat? http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStylesitem=EQS05 Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jsjurc...@comcast.net Sent: Friday, September 30, 2011 9:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alignment tool Who sells the microtome alignment tool that clamps onto the base of the microtome and also into the cassette holder to assure that all microtomes in the lab are in the same cutting plane? Do the bubble ones work as well? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Alignment tool
So does TechOne biomedical Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Friday, September 30, 2011 9:45 AM To: jsjurc...@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Alignment tool Newcomer sells one Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jsjurc...@comcast.net Sent: Friday, September 30, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alignment tool Who sells the microtome alignment tool that clamps onto the base of the microtome and also into the cassette holder to assure that all microtomes in the lab are in the same cutting plane? Do the bubble ones work as well? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Control Tissue
Hi all. Does anyone have a resource for control blocks? We are in need of fungus controls and are trying to save the cost of buying slides. Thanks all. Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc Sheila, National Society for Histotechnology has a Tissue Control Bank, If you contact the person below she can help: Melinda A Hamilton HT (ASCP) NSH Tissue Control Bank Curator Melinda Sokol m.soko...@gmail.com Hope that help! Star Bullard HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Position Available @ NIH (Bethesda, MD)
Seeking highly skilled scientist or histologist who is well versed in IHC. This position is in the National Institute of Mental Health (NIMH) and thus requires prior neuroscience and non-human primate experience. Please note that we are NOT necessarily seeking ASCP histology certificated individuals and that prior related experience will be preferred over certification. The position will support research in the NIMH. The position will provide histology support service for the complete laboratory (has 5 sections within it). This person will be the go to person for histology and IHC within the lab and its 5 sections. This individual will be expected to function as a resource to all of the lab's not solely as a technician but rather as a histology/IHC expert to advise the teams with their histology/IHC needs. This will be a collaborative research effort and will not offer personal independent research. Non-human primate model is the principal concern. Experience with IHC, Fluoro-ruby and green, D2, BrDu, tyrosine hyroxylase, perfusion, brain removal and prep, and much more is desired. If this opportunity is not right for you I ask that you point me in the direction of someone who may be appropriately qualified - maybe a colleague of yours, or former boss, or collaborator. The opportunity is a fulltime, salaried, indefinite, contractor position with benefits. Qualified candidates please submit a current CV/resume (in MS Word format) to jbla...@astrixinc.com. Thanks - Jason Blaine jbla...@astrixinc.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histonet Digest, Vol 94, Issue 40 Nothing down the drain
Yes nothing down the drain (except maybe the lithium carbonate bluing). Hematoxylin goes into medical waste service. Used Eosin gets reused a 10-20 ml at a time into the first absolute on the processors as a marker of carryover (as the later alcohols turn red, we have a visual indicator of the need to change, also helps with embedding since collagen stains more than epithelium/epidermis). Alcohols after the de-waxing xylenes and from the processor purges go to the medical waste service. Other alcohols get recycled. All the Xylenes get recycled. Liquid Waste from the recycler goes to medical waste service. Paraffins go into the trash. Steve Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] washing the placenta
Dear all, Is there someone with experience in washing of placentae before sampling? Regards Dr An Eerdekens ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
