[Histonet] Tlr 9 immuno
Hi All, Can somebody help me with ordering a TLR 9 antibody, which is working on mouse tissue, formalin fixed and paraffin embedded. Including the procedure and the name of the company. Greetings, Henk van de Kant Utrecht Institute of Pharmaceutical Sciences Faculty of Sciences, Utrecht University visiting address: Universiteitsweg 99, room 2.213584CG Utrecht, the Netherlands mailing address: PO Box 80.195, 3508TB Utrecht The Netherlands ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] decal [sic] question
funny, I thought a decal was a sort of transfer for decorating things with - like guitars and hot rods - what others call stickers hard to trademark a word like that! On Tue, Oct 4, 2011 at 5:38 PM, Bob Richmond rsrichm...@gmail.com wrote: Bernice Frederick HTL (ASCP), Senior Research Tech at the Pathology Core Facility of the Robert. H. Lurie Cancer Center at Northwestern University in Chicago notes Ann Preece states acid decal uses aqueous solutions of either formic, nitric, or trichloroacetic acid. Other methods mentioned are Ion-exchange resin, electrical ionization and chelation. The histo bible! You've got to be almost as geezer as me to remember when Ann Preece's A Manual for Histologic Technicians was the histo bible. I was fortunate to be able to purloin a pristine (no stain spills) copy of the third edition (1972) from the wreckage of an old histology lab about 20 years ago. Indeed, Patsy Ruegg! Decal is a trademark of the Decal Chemical Corporation and should not be used generically for decalcifying solutions. See decal-bone.com Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ann Preece (was decal [sic] question)
I have both a 1959 and 1972 copy of Preece's book! Both were left by the previous Histology Manager. What a treasurer for me. I also used Preece when studying for the registry. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Bob Richmond rsrichm...@gmail.com Sent by: histonet-boun...@lists.utsouthwestern.edu 10/04/2011 11:39 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Ann Preece (was decal [sic] question) Bernice Frederick HTL (ASCP), Senior Research Tech at the Pathology Core Facility of the Robert. H. Lurie Cancer Center at Northwestern University in Chicago notes Ann Preece states acid decal uses aqueous solutions of either formic, nitric, or trichloroacetic acid. Other methods mentioned are Ion-exchange resin, electrical ionization and chelation. The histo bible! You've got to be almost as geezer as me to remember when Ann Preece's A Manual for Histologic Technicians was the histo bible. I was fortunate to be able to purloin a pristine (no stain spills) copy of the third edition (1972) from the wreckage of an old histology lab about 20 years ago. Indeed, Patsy Ruegg! Decal is a trademark of the Decal Chemical Corporation and should not be used generically for decalcifying solutions. See decal-bone.com Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Paraffin sections for molecular assays
Hi! For our KRAS-testing we clean the microtome with LTK008, especially surfaces that come in contact with the sections. We use one-way-toothpicks, single packed, to pick the slide from the blade and give it directly into a sterile collection tube. 1-10 sections are collected depending on the dimensions of the tumorarea. In this way we don't have to deal with to-clean or not-to-clean water in the waterbath, brushes, forceps etc. Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Skin punch biopsy
Hi! I have 2mm skin punch biopsies that I need to process, but it has been a very long time since I have done this and don't really remember the times/station. These biopsies have been cut in half so they are extremely small - In other words, I am only planning on processing 1/2 of the biopsy punch. What do you guys recommend for a processing schedule? Thanks! Anna Hughes Anna C. Hughes Merck Co., Inc. anna_hug...@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Labvision Autostainer
Good afternoon fellow Histotechs! We have been using our Labvision Autostainer for 5 or so years now, and love the open system. Recently, we had a rep from Fisher come and do a PM on it, because we noticed the amount of reagent in some bottles was not changing at all, while others were running out very quickly. Today I took off a run of slides, and not one drop of DAB had been used. The stainer does not alarm, but its obviously not drawing up solution in some cases. Mr. Fisher rep said it tested fine. Has anyone else experienced this? We would greatly appreciate any insight or advice. Thanks so much! Laura Jones The Histochicks @ Sharon Regional Health System Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Isotype specific polymers
Hi everyone, I'm curious if there is such a thing as isotype specific polymers (ie will react with mouse IgG2a but not IgG1). Does anyone make such a thing? Thanks, Katy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4%
Ok I want to know the diferrence between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same thing and in the book from Frieda Carson it says that but my supervisor swears they are different chemicals. In the laboratory she has used 10% neutral buffered formalin all the time, but when we ran out of it we were sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB formaldehyde 3.7 to 4% once again. She says that 10% NBF is more concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are going to putrefied if they are put in that solution. Am I wrong or is she wrong? thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4%
Oh dear. Yes they are the same. Sent from my iPhone On Oct 5, 2011, at 4:03 PM, Jenny Vega histotech...@gmail.com wrote: Ok I want to know the diferrence between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same thing and in the book from Frieda Carson it says that but my supervisor swears they are different chemicals. In the laboratory she has used 10% neutral buffered formalin all the time, but when we ran out of it we were sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB formaldehyde 3.7 to 4% once again. She says that 10% NBF is more concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are going to putrefied if they are put in that solution. Am I wrong or is she wrong? thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4%
concentrated formaldehyde is 37-40% w/v so 10% formalin is 3.7-4% w/v.same thing.. yes..oh dear!! From: cha...@yahoo.com Date: Wed, 5 Oct 2011 16:05:31 -0700 To: histotech...@gmail.com Subject: Re: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% CC: histonet@lists.utsouthwestern.edu Oh dear. Yes they are the same. Sent from my iPhone On Oct 5, 2011, at 4:03 PM, Jenny Vega histotech...@gmail.com wrote: Ok I want to know the diferrence between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same thing and in the book from Frieda Carson it says that but my supervisor swears they are different chemicals. In the laboratory she has used 10% neutral buffered formalin all the time, but when we ran out of it we were sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB formaldehyde 3.7 to 4% once again. She says that 10% NBF is more concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are going to putrefied if they are put in that solution. Am I wrong or is she wrong? thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4%
Jenni, we do not oh dear you, in fact we congratulate you for coming here to get the answers you need. Many of us lament the state of affairs in histology today. Formaldehyde can not exist as a liquid. 100% Formaldehyde is a gas. To use it in histology we purchase it as formalin which has a concentration of 37% to a max of 40% formaldehyde. We then make 10% formalin which is 3.7% to 4% formaldehyde. Incidentally I have worked with a pathologist who used nothing but 4% zinc formalin very successfully (actually better penetration than 10%NBF) -- invalidating the initial premise of your supervisor. Will Sent from my iPhone On Oct 5, 2011, at 4:51 PM, Rosie Scrimizzi rosie_scrimi...@hotmail.com wrote: concentrated formaldehyde is 37-40% w/v so 10% formalin is 3.7-4% w/v.same thing.. yes..oh dear!! From: cha...@yahoo.com Date: Wed, 5 Oct 2011 16:05:31 -0700 To: histotech...@gmail.com Subject: Re: [Histonet] Difference between Neutral Buffered Formalin 10% andNB formaldehyde 3.7 to 4% CC: histonet@lists.utsouthwestern.edu Oh dear. Yes they are the same. Sent from my iPhone On Oct 5, 2011, at 4:03 PM, Jenny Vega histotech...@gmail.com wrote: Ok I want to know the diferrence between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same thing and in the book from Frieda Carson it says that but my supervisor swears they are different chemicals. In the laboratory she has used 10% neutral buffered formalin all the time, but when we ran out of it we were sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB formaldehyde 3.7 to 4% once again. She says that 10% NBF is more concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are going to putrefied if they are put in that solution. Am I wrong or is she wrong? thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hello everyone,After being home from the NSH for a few weeks I have been pondering an issue that I think bears discussion on the histonet.There have been several papers published regarding floaters and the amount determined to come from traditional staining buckets. There was also a poster presented at the NSH this year on the subject.When I approached several vendors of HE stainers about this issue. The answers were surprisingly pretty much the same. It is not an issue! Now I understand how one company can make this claim as their stainer uses fresh stain on each slide. The explanations from the other companies were insulting and just plain did not make sense to me. I was told by a Histo tech vendor that All Histo techs know that floaters come from the water bath. Well, she was talking to a histo tech and I know for a fact that floaters come from a variety of places. I have seen them from the doctor's office or procedure room to the stainer and every step in between. Sometimes if the floater is in the block it is very difficult to determine where it originated. We can however eliminate the water bath and stainer as the origin in these cases. One company told me that the design of the solution bottle eliminated floaters because floaters float and their stainer draws solutions from the bottom of the bottle. I have probably changed thousands of staining dishes during my 40+ year career (yes, I am old) and I have seen lots of little pieces of tissue at the bottom of the staining dishes. So, no, not all floaters float. I would love to hear feedback from others on this. I guess I would appreciate feedback about the floater issue as well as how a few vendors can make such claims and expect Histology techs to buy it. I really felt that a few comments were insulting to our profession and to the knowledge and expertise we possess. JanOmaha ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Difference between Neutral Buffered Formalin 10% andNB formaldehyde 3.7 to 4%
I considered mentioning the gas state of formaldehyde, and its saturation potential in aqueous solution, so I am glad to see this was posted.. However I was hesitant to reply right away b/c I too was sad that you were given this misinformation, especially since it is so easy to look up and verify. Good for you in not accepting an answer that did not seem correct and seeking more info! Sent from my Verizon Wireless BlackBerry -Original Message- From: William cha...@yahoo.com Date: Wed, 5 Oct 2011 23:59:25 To: rosie_scrimi...@hotmail.com Cc: histonet@lists.utsouthwestern.edu; histotech...@gmail.com; histotech...@gmail.com Subject: Re: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% Jenni, we do not oh dear you, in fact we congratulate you for coming here to get the answers you need. Many of us lament the state of affairs in histology today. Formaldehyde can not exist as a liquid. 100% Formaldehyde is a gas. To use it in histology we purchase it as formalin which has a concentration of 37% to a max of 40% formaldehyde. We then make 10% formalin which is 3.7% to 4% formaldehyde. Incidentally I have worked with a pathologist who used nothing but 4% zinc formalin very successfully (actually better penetration than 10%NBF) -- invalidating the initial premise of your supervisor. Will Sent from my iPhone On Oct 5, 2011, at 4:51 PM, Rosie Scrimizzi rosie_scrimi...@hotmail.com wrote: concentrated formaldehyde is 37-40% w/v so 10% formalin is 3.7-4% w/v.same thing.. yes..oh dear!! From: cha...@yahoo.com Date: Wed, 5 Oct 2011 16:05:31 -0700 To: histotech...@gmail.com Subject: Re: [Histonet] Difference between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% CC: histonet@lists.utsouthwestern.edu Oh dear. Yes they are the same. Sent from my iPhone On Oct 5, 2011, at 4:03 PM, Jenny Vega histotech...@gmail.com wrote: Ok I want to know the diferrence between Neutral Buffered Formalin 10% and NB formaldehyde 3.7 to 4% . I was taugh at college that they were the same thing and in the book from Frieda Carson it says that but my supervisor swears they are different chemicals. In the laboratory she has used 10% neutral buffered formalin all the time, but when we ran out of it we were sent NB formaldehyde 3.7 to 4 %she sent it back and we were sent NB formaldehyde 3.7 to 4% once again. She says that 10% NBF is more concentrated and pure than 3.7 to 4 % formaldehyde and that the tissues are going to putrefied if they are put in that solution. Am I wrong or is she wrong? thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
