Re: [Histonet] Looking for recommendations for a slide and cassette labeller system

2011-10-16 Thread Linda
Hi,
 
I use the Brady system, which has two dimension bar coding.  They also have a 
scan to print, which has a modem for wireless use.  The customer service is 
outstanding.  The price is pretty reasonable too.
 
 
Linda Dee, BGS, HT(ASCP)
 



From: Sheila Adey sa...@hotmail.ca
To: histonet@lists.utsouthwestern.edu
Sent: Saturday, October 15, 2011 7:06 PM
Subject: [Histonet] Looking for recommendations for a slide and cassette 
labeller system


Hello:We are looking into purchasing a slide and cassette labeller system. Any 
advice would be great. Also, if you know which system to stay away from, that 
would be appreciated aswell.ThanksSheila                         
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RE: [Histonet] (no subject)

2011-10-16 Thread Tony Henwood
Miha,

Are your muscles arising in saline?
Receive them dry or in cell culture media (eg Hanks).

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager  Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Miha Tesar
Sent: Saturday, 15 October 2011 7:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi!
I am working in Institute of Phatology Slovenia EU. Recently I have problems 
with muscle biopsy. There are a lots of artefact that I can not explain, like 
little holes in the tissue after the tissue is being frozen. We are using the 
isopentan and the liquid nitrogen for the freezing. Can enyone give me some 
ideas haw to avoid this artefact. How important is humidity in laboratory for 
the good results?
The next problem is that after I put the tissue on the microscope slides after 
coloring the GTK all the tissue gets wrinkeld. Iam using the poly L 
lisin microscope slides or the Immuno microscope slides.
Thx in advance!
Best regards Miha from SLO

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[Histonet] processing schedule for GI'S

2011-10-16 Thread anuradha shrivastava
Hello Histo gurus,
Could u please advice me on gi’s  processing schedule for Leica asp 300.
thanks in advance.
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Re: [Histonet] pouring of liquid nitrogen into a cryostat

2011-10-16 Thread Tony Reilly
Hi Louise
 
Not a very safe practice.  I have heard of people using a bag of dry ice 
pellets rested against the knife holder to lower the temperature of the knife, 
both for when the chamber is too warm or the tissue needs to be cut at a lower 
temperature, such as fat.
 
regards
Tony
 
 
 

Tony Reilly  B.App.Sc. , M.Sc.
Chief Scientist, Anatomical Pathology
Pathology Queensland-PA Laboratory
_
Clinical and Statewide Services Division| QueenslandHealth
 
Level 1, Building 15,Princess Alexandra Hospital
Ipswich Road,WOOLLOONGABBA  Qld4102
Ph: 07 3176 2412
Mob: 0402 139411
Fax: 07 3176 2930
Email: tony_rei...@health.qld.gov.au
Web:  www.health.qld.gov.au/qhcss/
 
 


 Louise Renton louise.ren...@gmail.com 10/15/2011 2:59 am 
bad idea - what about splash ups from the chamber? Very scary to contemplate

On Fri, Oct 14, 2011 at 6:36 PM, Kaye Ryan kr...@nfderm.com wrote:

 Hi Everyone,

 Has anyone ever heard of pouring liquid nitrogen into the chamber of a
 cryostat that has warmed up to help cool it down more quickly?  Does
 this do harm to the chamber or mechanisms of the cryostat?  Any
 information would be appreciated.



 Thanks

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-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel  fax)
073 5574456 (emergencies only)
Question: Are rhinos  overweight unicorns?
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[Histonet] Technovit 9100 New

2011-10-16 Thread Megan Silas
Hello,

Has anyone used Technovit 9100 New? If so, what company did you order
through - is there a distributor in the US? Also, have you found success
with this in embedding undecalcified bone?

Thanks for any and all advice!

Megan Silas

Megan Silas | megan.r.si...@gmail.com | 847-609-6336
University of Illinois at Urbana-Champaign | Bioengineering
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Re: [Histonet] Technovit 9100 New

2011-10-16 Thread Jack Ratliff
What is it you are trying to accomplish with staining? Can you tell us more 
about your project? I use Acrylosin SOFT Infiltration and Embedding solutions 
from Dorn and Hart Microedge (just up the road from you in Villa Park) for my 
thin 5 micron section and Acrylosin HARD for ground sections. Feel free to ask 
more questions as needed.


Jack


Senior Histologist, BioMimetic Therapeutics
Chairman, Hard Tissue Committee - National Society for Histotechnology



On Oct 16, 2011, at 10:47 PM, Megan Silas sil...@illinois.edu wrote:

 Hello,
 
 Has anyone used Technovit 9100 New? If so, what company did you order
 through - is there a distributor in the US? Also, have you found success
 with this in embedding undecalcified bone?
 
 Thanks for any and all advice!
 
 Megan Silas
 
 Megan Silas | megan.r.si...@gmail.com | 847-609-6336
 University of Illinois at Urbana-Champaign | Bioengineering
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