Re: [Histonet] problem with brain sections falling off subbed slides
Good morning. I see you mention only part of your protocol so I am not sure if you are going directly to the stain? This procedure seems close to what you are working with, it has a few tips. Hope this helps. Procedure: 1. Mount frozen or vibratomesections on gelatin coated or positive charged plus slides. Air dry sections or bake slides on slide warmer overnight. 2. Place slides directly into 1:1 alcohol/chloroform overnight and then rehydrate through 100% and 95 % alcohol to distilled water. DON’T put frozen sections directly to water otherwise they will come off the slides.This is called de-fat step that will reduce background fat staining. 3. Stain in 0.1% cresyl violet solution for 5-10 minutes. Notes:Staining in warmed cresyl violet solution (warm up in 37-50 ºC oven) can improve penetration and enhancing even staining. It is particularly beneficial for thicker (30 um) sections. 4. Rinse quickly in distilled water. 5. Differentiate in 95% ethyl alcohol for 2-30 minutes and check microscopically for best result. 6. Dehydrate in 100% alcohol 2x5 min. 7. Clear in xylene 2x5 min. 8. Mount with permanent mountingmedium. http://www.ihcworld.com/_protocols/special_stains/nissl-frozen-section.htm Kim Donadio From: John Kiernan jkier...@uwo.ca To: Douglas M Burns dmbur...@gmail.com; histonet@lists.utsouthwestern.edu Sent: Wednesday, November 2, 2011 11:19 PM Subject: Re: [Histonet] problem with brain sections falling off subbed slides Gelatin alone is not an adhesive because it dissolves in water. Chrome alum-gelatin is an excellent adhesive for sections and costs almost nothing to make. Subbed slides are one coated with chrome gelatin, dried and stored. The following web link shows a 1999 publication giving the rationale and methods for a variety of section adhesives. http://publish.uwo.ca/~jkiernan/adhesivs.htm John Kiernan Anatomy,UWO London, Canada = = = On 02/11/11, Douglas M Burns dmbur...@gmail.com wrote: Hello, I am new to working with brain, and I am having a bad problem with both thin and thick rat brain sections sliding off of subbed slides. We tried commercial treated slides first, but then moved on to subbing them. The subbing was done with gelatin according to a printed protocol, and I can see the film on the dried slide. When kept horizontal they seem okay, but as soon as they are put into a slide holder for washing or anything, they begin to slide off or even move around. After a short while, many/most of mysteriously vanished (into the wash solution). Our slices are sliced by cryostat from a rat brain that has been fixed with 4% paraformaldehyde and 30% sucrose. The slice comes right off the cryostat, and then I m ove it onto a warm slide. (Perhaps they are not warm enough?? I have seen others blow on them to warm them.) Slides destined for Cresyl violet staining were dried for 3 days at room temperature before being moved. It is the Cresyl violet protocol that causes the most trouble, but I am seeing some problems with any type of large volume wash. At this point, I don't have enough experience with this specific tissue and our protocols to be able to tell for sure what is happening. Thus, I am entirely open to advice - any advice that might help with the problem. Thank you - Doug Burns, Kansas City ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] problem with brain sections falling off subbed slides
Apart from Dr. Kiernan's recommendation, you can try putting your mounted slides in fixative for a few minutes, we've found this to work with gelatin-only coated slides, or chrome-alum gelatin subbed slides with thick brain sections. If you're trying to rescue material already on slides, dip briefly in warm 1% gelatin, air dry, fix for a few minutes in 10% NBF, and proceed to counterstaining. --- Message: 14 Date: Wed, 2 Nov 2011 17:06:48 -0500 From: Douglas M Burns dmbur...@gmail.com Subject: [Histonet] problem with brain sections falling off subbed slides Hello, I am new to working with brain, and I am having a bad problem with both thin and thick rat brain sections sliding off of subbed slides... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Specimen Tracking for courier
I'm curious what methods other labs are using to track specimens that couriers pick up from clinic locations to ensure safe delivery to the lab. We currently utilize a logbook at the clinic site that the courier signs when they take the specimen but I have heard there are barcoded systems available out there as well. What works? What doesn't? Appreciate your feedback! Jenny mailgate.hinet.org made the following annotations - NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Specimen Tracking for courier
I would be interested in everyone responses. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manger X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bull, Jennifer L. Sent: Thursday, November 03, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Specimen Tracking for courier I'm curious what methods other labs are using to track specimens that couriers pick up from clinic locations to ensure safe delivery to the lab. We currently utilize a logbook at the clinic site that the courier signs when they take the specimen but I have heard there are barcoded systems available out there as well. What works? What doesn't? Appreciate your feedback! Jenny mailgate.hinet.org made the following annotations - NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Specimen Tracking for courier
Our lab as a whole uses the Gajema Specimen Transport And Tracking System for all courier deliveries Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager Nationwide Children's Hospital Columbus OH 43205 (614) 722 5450 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Thursday, November 03, 2011 11:13 AM To: 'Bull, Jennifer L.'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Specimen Tracking for courier I would be interested in everyone responses. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manger X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bull, Jennifer L. Sent: Thursday, November 03, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Specimen Tracking for courier I'm curious what methods other labs are using to track specimens that couriers pick up from clinic locations to ensure safe delivery to the lab. We currently utilize a logbook at the clinic site that the courier signs when they take the specimen but I have heard there are barcoded systems available out there as well. What works? What doesn't? Appreciate your feedback! Jenny mailgate.hinet.org made the following annotations - NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Specimen Tracking for courier
Jenny, I know Triangle Urology Associates in NC is using TBS' SHUR/Mark-TVT tracking and verification system and love it! If you would like additional info I will be happy to get it for you? Dustin Campbell -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Thursday, November 03, 2011 11:13 AM To: 'Bull, Jennifer L.'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Specimen Tracking for courier I would be interested in everyone responses. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manger X-Cell Laboratories e-mail cp...@x-celllab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bull, Jennifer L. Sent: Thursday, November 03, 2011 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Specimen Tracking for courier I'm curious what methods other labs are using to track specimens that couriers pick up from clinic locations to ensure safe delivery to the lab. We currently utilize a logbook at the clinic site that the courier signs when they take the specimen but I have heard there are barcoded systems available out there as well. What works? What doesn't? Appreciate your feedback! Jenny mailgate.hinet.org made the following annotations - NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Staffing to work ratios
Hi, I know this subject has been discussed before, but I'm having trouble finding the info I need from the archives. Would anyone know where I can find the work to staffing ratios for a Histology laboratory? Our volume was ~9750 surgical cases (2010). The majority of them are biopsies (GI, GYN, skin, etc) but we do some larger complex cases. We had 2569 billable IHC tests, 254 billable group I special stains, 722 billable group II special stains. #blocks: 23,380 # HE slides : 49,524 Thank you for your help! Stacy McLaughlin, HT(ASCP) Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton, MA 01060 (413)582-2019 stacy_mclaugh...@cooley-dickinson.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Names of Hospitals with Histology in Alabama
Fishing for some Names in Alabama that have Histology Labs? Thanks Donna Hunter HTII CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Training and Competency Assessment for HE Slide Review
Any suggestions for creating a Training and Competency (TC) Assessment for HE slide review? We currently QC all HE slides macroscopically and 15% microscopically. I would like to have all Histotechs trained and competent to QC HE slides. Has anyone gone thru this process? Thanks Diana Harris QC Method Development Technologist Royal Jubilee Hospital Victoria, BC Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Training and Competency Assessment for HE Slide Review
Any certified histologist has gone through this, but the NSH has good resources for this. Sent from my Verizon Wireless BlackBerry -Original Message- From: Harris Diana diana.har...@viha.ca Date: Thu, 3 Nov 2011 17:34:03 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Training and Competency Assessment for HE Slide Review Any suggestions for creating a Training and Competency (TC) Assessment for HE slide review? We currently QC all HE slides macroscopically and 15% microscopically. I would like to have all Histotechs trained and competent to QC HE slides. Has anyone gone thru this process? Thanks Diana Harris QC Method Development Technologist Royal Jubilee Hospital Victoria, BC Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Brain sections...
This works if you are doing traditional specials--never worked out if it affected IHC. We used it for autopsy brained -called it double cook--and you can't scratch the tissue off with a knife after they're handled like this. Dry your slides in low heat the way you always would. Then cool completely. Heat them again til the wax melts and the slide is up to the higher temp (about 10 minutes). Cool and handle as usual. Something about the expanding and contracting of going in and out of the heat twice 'snuggles' the tissue onto the slide. Cheryl Kerry, HT(ASCP) Full Staff Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Brain sections...
And I've always found that letting them air dry overnight before drying in the oven works well too. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Thursday, November 03, 2011 15:08 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Brain sections... This works if you are doing traditional specials--never worked out if it affected IHC. We used it for autopsy brained -called it double cook--and you can't scratch the tissue off with a knife after they're handled like this. Dry your slides in low heat the way you always would. Then cool completely. Heat them again til the wax melts and the slide is up to the higher temp (about 10 minutes). Cool and handle as usual. Something about the expanding and contracting of going in and out of the heat twice 'snuggles' the tissue onto the slide. Cheryl Kerry, HT(ASCP) Full Staff Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
