[Histonet] Stain Dye Stability Article

2011-12-19 Thread Breeden, Sara
I've received permission to send a copy of the article from the
Biological Stain Commission on Stain  Dye Stability to those who asked.
If you are one of those several individuals who asked me to send you a
copy, please contact me directly and I'll attach and send to you ASAP.

 

Sally Breeden, HT(ASCP)

New Mexico Department of Agriculture

Veterinary Diagnostic Services

1101 Camino de Salud NE

Albuquerque, NM  87102

505-383-9278 (Histology Lab)

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] Raymond Lamb Microwriter I

2011-12-19 Thread Amy Self
Good Morning,

I am looking for some help from someone that has used the Raymond Lamb 
Microwriter I cassette writer. We currently have one (and yes we desperately 
need to upgrade) that gives us trouble daily. The cassettes do not want to 
release from the plastic tubing into the cassette holding arm. Then there are 
times the arm will continuously open and close and never drop a cassette down. 
If there is anyone out there that has this type cassette writer that could 
possibly help me out please contact me.  Thanks in advance for your help, Amy


Amy Self
Georgetown Hospital System
843-527-7179
NOTE:
 The information contained in this message may be privileged, confidential and 
protected from disclosure. If the reader of this message is not the intended 
recipient, or an employee or agent responsible for delivering this message to 
the intended recipient, you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. If you 
have received this communication in error, please notify us immediately by 
replying to this message and deleting it from your computer.
Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] Has anyone else noticed

2011-12-19 Thread Della Speranza, Vinnie
 
Wondering if anyone else has noticed that rinsing a wine glass with water will 
turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin 
staining solution) ??


Yes, with so little positive in the news these days, I am definitely 
anesthetizing myself this holiday season.  

Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974

 



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] RE: Has anyone else noticed

2011-12-19 Thread Edwards, Richard E.
Sounds to me  as  if you  are wasting a few too many drops there!!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della Speranza, 
Vinnie
Sent: 19 December 2011 15:24
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Has anyone else noticed

 
Wondering if anyone else has noticed that rinsing a wine glass with water will 
turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin 
staining solution) ??


Yes, with so little positive in the news these days, I am definitely 
anesthetizing myself this holiday season.  

Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974

 



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] Re: Histonet Digest, Vol 97, Issue 21

2011-12-19 Thread gayle callis
This same clone for Rat CD31 still sold by BD 
Biosciences/Pharmingen/Invitrogen, also Serotec, ABCAM and a  company called 
Research Diagnostics found in the IHC World protocol.   The latter protocol 
used Proteinase K digestion.   

Gayle Callis 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madeleine Huey
Sent: Sunday, December 18, 2011 9:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 21

Colleen,

Many years ago I used Pharmingen's  anti-Rat CD31, clone TLD-3A12 (I think, you 
need to check it out if they still sell it).

This antibody is not recommend for formalin fixed paraffin tissues, but it will 
work.

Following steps are critical;
1) Do not use HIER, use enzymatic digestion (need optimization with Trypsin or 
Proteinase K @ 37c.  Lot to Lot variation from manufacture)
2) Overnight Incubation @ RT, not 4C (need warmer  long time)
3) Do not over dilute the 1st ab (ie. 1:10 - 1:25)
4) Try use a more sensitive detection system (Leica Refine DAB system) Good 
Luck!
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor-Pathology, IPOX  Histology
El Camino Hospital
madelein...@elcaminohospital.org


On Sun, Dec 18, 2011 at 10:00 AM,
histonet-requ...@lists.utsouthwestern.edu wrote:
 Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

 To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu

 You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

 When replying, please edit your Subject line so it is more specific 
 than Re: Contents of Histonet digest...


 Today's Topics:

   1. Re: Histonet Digest, Vol 97, Issue 20 (Madeleine Huey)
   2. RE: CD31 for rat tissue (Patsy Ruegg)
   3. e-cadherin (Patsy Ruegg)
   4. RE:   mouse antiRat CD31 (gayle callis)


 --

 Message: 1
 Date: Sat, 17 Dec 2011 13:28:52 -0800
 From: Madeleine Huey madeleineh...@gmail.com
 Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 20
 To: histonet@lists.utsouthwestern.edu
 Message-ID:

 CAF2e4C+Xe45xiKcGK0D_4HcEN2kMbNT=l67w2rekln_unks...@mail.gmail.com
 Content-Type: text/plain; charset=UTF-8

 Magan,

 Your problem is very simple.  First of all, you do not need Harris 
 Hematoxylin after IHC, because Harris Hematoxylin is a regressive 
 hematoxylin.  What you need is a progressive hematoxylin, like Mayer, 
 Gill (I, II, III)  etc.

 Try this simple procedure after your DAB chromogen;
 1) Counterstain in Gill I (Sigma) or Mayer (American MasterTech) for
 0.5 - 1 min (longer if want darker counterstain, or use Gill II/III.
 Personal preferences)
 2) Wash off excess Hematoxylin with tap water (no need distilled 
 water, your experiment is done)
 3) Blue the Nuclei with PBS or TBS buffer (common buffers used by IHC)
 4) Wash with water
 5) Dehydrate  cover slip with permanent mounting

 You can write or call me if you still need help or have any questions.

 Madeleine Huey BS, HTL (ASCP) QIHC
 Supervisor-Pathology
 El Camino Hospital
 Mountain View, CA
 madelein...@elcaminohospital.org

 On Sat, Dec 17, 2011 at 10:00 AM,
 histonet-requ...@lists.utsouthwestern.edu wrote:
 Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

 To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu

 You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

 When replying, please edit your Subject line so it is more specific 
 than Re: Contents of Histonet digest...


 Today's Topics:

   1. 2% low melting agarose gel (awatan...@tgen.org)
   2. Re: 2% low melting agarose gel (Emily Sours)
   3. AUTO: Ramona Nelson is out of the office. (returning
  01/03/2012) (ramona_nel...@bd.com)
   4. Formalin cost account (Morken, Timothy)
   5. Looking for a used Biocare Decloaker (Sowmya Kedarnath)
   6. Re: Looking for a used Biocare Decloaker (Akemi Allison)
   7. CD31 for rat tissue (Colleen Forster)
   8. Re: CD31 for rat tissue (Lucie Guernsey)
   9. Diane Tokugawa/CA/KAIPERM is out of the office.
  (diane.tokug...@kp.org)
  10. paraffin recycler (Gudrun Lang)
  11. Re: paraffin recycler (Rene J Buesa)
  12. Re: DAB haematoxylin counterstain;too purple, 
 overpowering
  IHC (Maxim Peshkov)
  13. Re: New Lab (mequita praet)


 -
 -

 Message: 1
 Date: Fri, 16 Dec 2011 18:12:01 +
 From: awatan...@tgen.org
 Subject: [Histonet] 2% low melting agarose gel
 To: 

FW: [Histonet] Has anyone else noticed

2011-12-19 Thread Della Speranza, Vinnie



From: Pam Barker [mailto:rel...@earthlink.net]
Sent: Monday, December 19, 2011 10:57 AM
To: Della Speranza, Vinnie
Subject: RE: [Histonet] Has anyone else noticed

hahahahaha Vinnie!
A nice Cab is a great anesthetic, and your observation is a great reason to 
open a bottle!!  Who's with me?  Happy Holidays!!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
histonet-boun...@lists.utsouthwestern.edu
Sent: 12/19/2011 10:31 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Has anyone else noticed



Wondering if anyone else has noticed that rinsing a wine glass with water will 
turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin 
staining solution) ??


Yes, with so little positive in the news these days, I am definitely 
anesthetizing myself this holiday season.

Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974





___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] RE: Has anyone else noticed

2011-12-19 Thread Blazek, Linda
Boy, that was my thought!  

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards, 
Richard E.
Sent: Monday, December 19, 2011 10:41 AM
To: 'Della Speranza, Vinnie'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Has anyone else noticed

Sounds to me  as  if you  are wasting a few too many drops there!!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della Speranza, 
Vinnie
Sent: 19 December 2011 15:24
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Has anyone else noticed

 
Wondering if anyone else has noticed that rinsing a wine glass with water will 
turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin 
staining solution) ??


Yes, with so little positive in the news these days, I am definitely 
anesthetizing myself this holiday season.  

Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974

 



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] Raymond Lamb Microwriter I

2011-12-19 Thread Dustin Paul Campbell
Amy,

Unfortunately, Raymond Lamb was bought out by Thermo Fisher years ago and they 
no longer support existing cassette/Slide labelers. However, TBS will be happy 
to support you with any issues. We picked up the existing line of cassette and 
slide labelers from Thermo and we sell and support the product. Call the 
following number and ask for the service department 919-384-9393.

Hope this helps,


Dustin Campbell   
Project Manager - SHUR/Mark-TVT(tm) 

  Triangle Biomedical Sciences, Inc.
3014 Croasdaile Drive *  Durham   NC  27705  *  phone 919.384.9494 ext. 188  
* fax 919.384.9595
dcampb...@trianglebiomedical.com *  www.trianglebiomedical.com * TBS' 
Comprehensive Product Catalog 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self
Sent: Monday, December 19, 2011 9:39 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Raymond Lamb Microwriter I

Good Morning,

I am looking for some help from someone that has used the Raymond Lamb 
Microwriter I cassette writer. We currently have one (and yes we desperately 
need to upgrade) that gives us trouble daily. The cassettes do not want to 
release from the plastic tubing into the cassette holding arm. Then there are 
times the arm will continuously open and close and never drop a cassette down. 
If there is anyone out there that has this type cassette writer that could 
possibly help me out please contact me.  Thanks in advance for your help, Amy


Amy Self
Georgetown Hospital System
843-527-7179
NOTE:
 The information contained in this message may be privileged, confidential and 
protected from disclosure. If the reader of this message is not the intended 
recipient, or an employee or agent responsible for delivering this message to 
the intended recipient, you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. If you 
have received this communication in error, please notify us immediately by 
replying to this message and deleting it from your computer.
Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] Has anyone else noticed

2011-12-19 Thread Janice Mahoney

I have noticed it many many times.
Happy Holidays
Jan Mahoney, Omaha
 

 From: del...@musc.edu
 To: histonet@lists.utsouthwestern.edu
 Date: Mon, 19 Dec 2011 10:23:40 -0500
 Subject: [Histonet] Has anyone else noticed
 
 
 Wondering if anyone else has noticed that rinsing a wine glass with water 
 will turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin 
 staining solution) ??
 
 
 Yes, with so little positive in the news these days, I am definitely 
 anesthetizing myself this holiday season. 
 
 Vinnie Della Speranza, MS, HTL(ASCP)
 Manager for Anatomic Pathology Services
 Medical University of South Carolina
 165 Ashley Avenue MSC 908
 Charleston, SC 29425
 tel. 843-792-6353
 fax. 843-792-8974
 
 
 
 
 
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] Biocare Decloaker

2011-12-19 Thread Sheila Fonner
Would the person asking about a used Biocare Decloaker please contact me.
We have one that is like new. No longer use it since all retrieval is online
with automated stainer.

Would be willing to sell.

 

Thanks,

Sheila

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] Supervisor postion at UC San Francisco

2011-12-19 Thread Morken, Timothy
We are still looking for the right person for the Histology Supervisor postion 
at University of California San Francisco Medical Center. The job description 
is below with the careers website url. Search for histotechnologist to see 
the supervisor listing.

UCSF is soley a medical institution. Pathology has 25 residents and total of 60 
pathologists, including research in the medical school. We do exentive research 
support in the histology lab.

The Histology / Immunohistochemistry supervisor oversees general histology 
(tissue processing, embedding, sectioning, staining), specials stains and 
immunohistochemistry. The position does not cover grossing/frozen sections, 
transcription or cytology.

We work with 12 pathologists directly and many more indirectly. There are 12 
histotechnologists in the lab, the majority are ASCP-certified. The case load 
is 30,000 per year, 130,000 blocks/year (about 500+ per day average). Immuno 
does over 250 slides per day, up to 350. We are undergoing a transition to 
fully automated instrumentation.

The supervisor will be highly involved in process improvment, LEAN processes, 
planning implementation of a continuous processing schedule, selection of new 
euipment, including HE stainers, immuno stainers, tissue processors, etc, 
selection and implementation of specimen tracking barcoding system.

The major project in the next two years is the design and build-out of a new 
laboratory at a new location near the new Mission Bay hospital complex that is 
under construction.

This is a very busy place and never a dull moment. The staff is extremely 
dedicated and support from the Medical Directors is excellent as well.


If interested please apply at the website below and contact me with any 
questions at tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org

Tim Morken
Supervisor, Histology/IHC (soon to be supervisor Electron Microscopy - once 
this postion is filled!)


Histology, Immunohistochemistry Supervisor postion at University of California 
San Francisco Medical Center


https://careers.ucsfmedicalcenter.orghttps://careers.ucsfmedicalcenter.org/

Job ID 2651

At the University of California San Francisco Medical Center, teamwork and 
continual learning have maintained our top 10 ranking as one of  America's 
Best Hospitals by U.S.News  World Report. For you, UCSF Medical Center is 
where you will share and discover something new every day with benefits and 
rewards that will last a lifetime.

Job Summary:
Under general direction from the Medical Director, the incumbent serves as 
supervisor to 3 Lead Histotechnologists and 9 Histotechnologists.   In addition 
to supervisory duties, the incumbent serves as a technical expert providing 
direction to staff as well as performing all technical aspects of surgical 
histology and immunohistochemistry procedures.  As supervisor:  recruits, 
hires, trains, completes performance evaluations, and resolves employee issues. 
 Provides orientation, completes competency assessments, maintains staff 
schedules, training and compliance documentation.  Implements new tests and 
procedures as requested by Medical Directors.  Updates and maintains the lab 
manuals and annual reviews required for accreditation.  Integrates new 
technologies into the laboratory to include analyzing and selecting of new 
equipment that meets space constraints and operational needs.  Ensures that new 
equipment and associated validation and staff training are performed.  Ensures 
that all equipment is well-maintained.  Ensures that staff training and 
documentation are completed as required.  Ensures that QC documentation is 
complete and quality standards are maintained in the laboratory.  Advises and 
assists researchers planning research projects and determines the ability of 
the laboratory to accommodate research projects, updating the Medical Director 
as required.  Other duties as assigned.

Required Qualifications:
College degree in a biological science, chemistry or a related field or 
equivalent education and work experience experience as a histotechnologist in a 
comparable high-volume hospital histology laboratory within the last seven 
years, including senior-level experience.  Demonstrated high-volume, 
high-quality sectioning and staining skills.  Ability to organize and 
prioritize responsibilities and perform well under pressure to meet deadlines.  
Excellent interpersonal and communication skills.   ASCP certification:  HT 
licensed or eligible required (candidate will be expected to obtain 
certification within 1 year), HTL desirable.  Excellent interpersonal 
communication skills required.  Demonstrated ability to organize and prioritize 
responsibilities and perform well under pressure to meet deadlines required.  
Previous, recent supervisory experience in a Histology and /or Immunoperoxidase 
Laboratory required.  Must be experienced with information technology systems 
and the use of hardware and software in business and 

[Histonet] used seymour IHC printer

2011-12-19 Thread Patsy Ruegg
Does anyone have a used Seymour printer I could purchase as a back up?  I
think I can use my installation disc but it has one I would want that to.

 

Regards,

Patsy

 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email  mailto:pru...@ihctech.net pru...@ihctech.net
web site  http://www.ihctech.net www.ihctech.net

 


This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged  confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] Has anyone else noticed

2011-12-19 Thread Tony Henwood (SCHN)
I keep trying to verify this scientific discovery but everything is blurry by 
the time I get to the bottom of the bottle, Oh sorry I need to look in the 
glass?

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager  Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della Speranza, 
Vinnie
Sent: Tuesday, 20 December 2011 3:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: FW: [Histonet] Has anyone else noticed




From: Pam Barker [mailto:rel...@earthlink.net]
Sent: Monday, December 19, 2011 10:57 AM
To: Della Speranza, Vinnie
Subject: RE: [Histonet] Has anyone else noticed

hahahahaha Vinnie!
A nice Cab is a great anesthetic, and your observation is a great reason to 
open a bottle!!  Who's with me?  Happy Holidays!!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
histonet-boun...@lists.utsouthwestern.edu
Sent: 12/19/2011 10:31 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Has anyone else noticed



Wondering if anyone else has noticed that rinsing a wine glass with water will 
turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin 
staining solution) ??


Yes, with so little positive in the news these days, I am definitely 
anesthetizing myself this holiday season.

Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974





___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*
This email and any files transmitted with it are confidential and intended 
solely for the use of the individual or entity to whom they are addressed. If 
you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual 
sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been virus scanned and 
although no computer viruses were detected, The Childrens Hospital at Westmead 
accepts no liability for any consequential damage resulting from email 
containing computer viruses.
*

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


[Histonet] bacteria staining

2011-12-19 Thread mesruh turkekul
I would like to stain FFPE mouse intestine sections to demonstrate most of
the bacteria in the normal flora of the intestine.
Any suggestions for the simplest histological stain that will show most of
the bacteria?

Thanks,
Happy holidays!

Mesru
mskcc.org
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] bacteria staining

2011-12-19 Thread Tony Henwood (SCHN)
Mesru,

Use 1% aqueous Giemsa, stain dehydrated sections 5-10 minutes, dehydrate 
rapidly (either acetone, air dry or rapid alcohol dehydation), clear and mount

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager  Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of mesruh turkekul
Sent: Tuesday, 20 December 2011 9:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] bacteria staining

I would like to stain FFPE mouse intestine sections to demonstrate most of the 
bacteria in the normal flora of the intestine.
Any suggestions for the simplest histological stain that will show most of the 
bacteria?

Thanks,
Happy holidays!

Mesru
mskcc.org
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*
This email and any files transmitted with it are confidential and intended 
solely for the use of the individual or entity to whom they are addressed. If 
you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual 
sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been virus scanned and 
although no computer viruses were detected, The Childrens Hospital at Westmead 
accepts no liability for any consequential damage resulting from email 
containing computer viruses.
*

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] Has anyone else noticed

2011-12-19 Thread Della Speranza, Vinnie
Actually Tony, by the time you get to the bottom of the bottle, you probably no 
longer care what color it is ! 


Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974

 


-Original Message-
From: Tony Henwood (SCHN) [mailto:tony.henw...@health.nsw.gov.au] 
Sent: Monday, December 19, 2011 5:04 PM
To: Della Speranza, Vinnie; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Has anyone else noticed

I keep trying to verify this scientific discovery but everything is blurry by 
the time I get to the bottom of the bottle, Oh sorry I need to look in the 
glass?

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager  Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della Speranza, 
Vinnie
Sent: Tuesday, 20 December 2011 3:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: FW: [Histonet] Has anyone else noticed




From: Pam Barker [mailto:rel...@earthlink.net]
Sent: Monday, December 19, 2011 10:57 AM
To: Della Speranza, Vinnie
Subject: RE: [Histonet] Has anyone else noticed

hahahahaha Vinnie!
A nice Cab is a great anesthetic, and your observation is a great reason to 
open a bottle!!  Who's with me?  Happy Holidays!!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
histonet-boun...@lists.utsouthwestern.edu
Sent: 12/19/2011 10:31 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Has anyone else noticed



Wondering if anyone else has noticed that rinsing a wine glass with water will 
turn a nice cabernet sauvignon residue blue (reminiscient of hematoxylin 
staining solution) ??


Yes, with so little positive in the news these days, I am definitely 
anesthetizing myself this holiday season.

Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974





___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*
This email and any files transmitted with it are confidential and intended 
solely for the use of the individual or entity to whom they are addressed. If 
you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual 
sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been virus scanned and 
although no computer viruses were detected, The Childrens Hospital at Westmead 
accepts no liability for any consequential damage resulting from email 
containing computer viruses.
*

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Re: [Histonet] Has anyone else noticed

2011-12-19 Thread Joe Nocito


I wonder if it works with Scotch. Hmmm, seems like an experiment brewing.
JTT

- Original Message - 
From: Tony Henwood (SCHN) tony.henw...@health.nsw.gov.au
To: 'Della Speranza, Vinnie' del...@musc.edu; 
histonet@lists.utsouthwestern.edu

Sent: Monday, December 19, 2011 4:04 PM
Subject: RE: [Histonet] Has anyone else noticed


I keep trying to verify this scientific discovery but everything is blurry 
by the time I get to the bottom of the bottle, Oh sorry I need to look in 
the glass?


Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager  Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della 
Speranza, Vinnie

Sent: Tuesday, 20 December 2011 3:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: FW: [Histonet] Has anyone else noticed




From: Pam Barker [mailto:rel...@earthlink.net]
Sent: Monday, December 19, 2011 10:57 AM
To: Della Speranza, Vinnie
Subject: RE: [Histonet] Has anyone else noticed

hahahahaha Vinnie!
A nice Cab is a great anesthetic, and your observation is a great reason to 
open a bottle!!  Who's with me?  Happy Holidays!!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
histonet-boun...@lists.utsouthwestern.edu

Sent: 12/19/2011 10:31 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Has anyone else noticed



Wondering if anyone else has noticed that rinsing a wine glass with water 
will turn a nice cabernet sauvignon residue blue (reminiscient of 
hematoxylin staining solution) ??



Yes, with so little positive in the news these days, I am definitely 
anesthetizing myself this holiday season.


Vinnie Della Speranza, MS, HTL(ASCP)
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue MSC 908
Charleston, SC 29425
tel. 843-792-6353
fax. 843-792-8974





___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*
This email and any files transmitted with it are confidential and intended 
solely for the use of the individual or entity to whom they are addressed. 
If you are not the intended recipient, please delete it and notify the 
sender.


Views expressed in this message and any attachments are those of the 
individual sender, and are not necessarily the views of The Children's 
Hospital at Westmead


This note also confirms that this email message has been virus scanned and 
although no computer viruses were detected, The Childrens Hospital at 
Westmead accepts no liability for any consequential damage resulting from 
email containing computer viruses.

*

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 



___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet