[Histonet] MiTF
Happy Holidays Histoland, Does anyone out there have a good working protocol on the Ventana Ultra for MiTF. I have tried several but the docs still aren't happy with it. Could you share any info you may have regarding vendor, system, protocol, etc.? Much thanks! Sheila, HT (ASCP) KDL Pathology Knoxville, TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech position
We are currently recruiting a histotech for our small independent pathology lab located in South Carolina. We offer a competitive salary, health insurance, sick leave, paid vacation, holidays, 401K/profit sharing. The hours are 5am-1pm. The job would include but not limited to embedding, cutting, staining, and knowledge of immunohistochemistry. Please call or email me if you are interested. Thanks! Deborah Taylor, MS, CT(ASCP) Customer Relations/Lab Manager Marlboro Chesterfield Pathology, PC 672 Hwy 9 West Bennettsville, SC 29512 Phone: 843-479-2402 Fax: 843-479-6609 Note: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anone else is unauthorized. If you are not the intended recipient, any disclosure copying or distribution of the message or any action or omission taken by you in reliance on it, is prohibitied and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Coverslip Sealant
Hi there, Does anybody know of a good alternative to nail polish for sealing round coverslips (or any shape, for that matter!). Ideally I am looking for a solution that is both an aqueous mount media and sealant, if such a beast exists... Thanks! Gil PS: Happy New Year! -- Guillermo Palchik Ph.D. Candidate - Interdisciplinary Program in Neuroscience Georgetown University Medical Center Research Building Room W 217 3970 Reservoir Rd. NW, Washington, DC 20007 Lab: 202-687-7825 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC on Bones
Hello Histonet, Wondering what those of you that do IHC on bones do to ensure that the tissue remains on the slide during processing? Thanks, Sara McCabe HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IHC on Bones
Sara If you can you want to stick with an enzyme digestion such as proteinase K or another enzyme. If the antibody requires HIER and will not work with enzyme digestion then modify your retreival time and temp, we use 70C for 2 hours and find that this works for most antibodies. Some antibodies that require HIER may not work with the longer/lower temp methods. Good Luck Liz From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McCabe, Sara J. [sjmcc...@drmc.org] Sent: Friday, December 30, 2011 11:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on Bones Hello Histonet, Wondering what those of you that do IHC on bones do to ensure that the tissue remains on the slide during processing? Thanks, Sara McCabe HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC on Bones
pray. McCabe, Sara J. sjmcc...@drmc.org 12/30/2011 1:02 PM Hello Histonet, Wondering what those of you that do IHC on bones do to ensure that the tissue remains on the slide during processing? Thanks, Sara McCabe HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IHC on Bones
I have place the deparaffinized slides into 10% neutral buffered formalin for about 30 minutes before the heat retrieval. Somehow this seems to work You can also lower the temperature of your retrieval and lengthen the time. Good Luck. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McCabe, Sara J. [sjmcc...@drmc.org] Sent: Friday, December 30, 2011 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on Bones Hello Histonet, Wondering what those of you that do IHC on bones do to ensure that the tissue remains on the slide during processing? Thanks, Sara McCabe HT(ASCP) DuBois Regional Medical Center This email and any attached files are confidential and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of the company. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NCCI policy update
Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC cocktail stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: NCCI policy update
Oh yes, we are very aware and quite upset at the change! Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, December 30, 2011 1:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] NCCI policy update Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC cocktail stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Coverslip Sealant
ProLong Gold (Invitrogen) Montina J. Van Meter, HT (ASCP) Lab Manager Dept. of Autonomic Neuroscience Pennington Biomedical Research Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Guillermo Palchik Sent: Friday, December 30, 2011 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coverslip Sealant Hi there, Does anybody know of a good alternative to nail polish for sealing round coverslips (or any shape, for that matter!). Ideally I am looking for a solution that is both an aqueous mount media and sealant, if such a beast exists... Thanks! Gil PS: Happy New Year! -- Guillermo Palchik Ph.D. Candidate - Interdisciplinary Program in Neuroscience Georgetown University Medical Center Research Building Room W 217 3970 Reservoir Rd. NW, Washington, DC 20007 Lab: 202-687-7825 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: NCCI policy update
Where can I get the article to show my billing dept/pathologists? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Friday, December 30, 2011 1:13 PM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: NCCI policy update Oh yes, we are very aware and quite upset at the change! Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, December 30, 2011 1:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] NCCI policy update Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC cocktail stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] NCCI policy update
I would expect the trend to go toward specimen and not block, perhaps even case and not specimen. It comes down to health care cost reduction and health-care reform which are turning out to be the same thing. I don' care who's holding the wheel, so long as gov't is doing the driving, we can expect this sort of thing. - Have a great new year - Bill -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, December 30, 2011 12:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] NCCI policy update Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC cocktail stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents is intended solely for the named addressee(s) and contains confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: NCCI policy update
Wow. What made them change their minds? There are many times when we stain different blocks of a single specimen and only one lights up. I, too, would be interested in an official document stating the change. Thanks! Michelle -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Friday, December 30, 2011 3:07 PM To: Martha Ward-Pathology; Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: NCCI policy update Where can I get the article to show my billing dept/pathologists? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Friday, December 30, 2011 1:13 PM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: NCCI policy update Oh yes, we are very aware and quite upset at the change! Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, December 30, 2011 1:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] NCCI policy update Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC cocktail stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: 12/30/11 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: NCCI policy update
Don't you think we havea hand in this reporting out this CPT code so much,, I would expect this to be bundled here soon in a DRG for pathology services,, that's where it is headed. Sent from my iPad On Dec 30, 2011, at 2:22 PM, histot...@imagesbyhopper.com histot...@imagesbyhopper.com wrote: Wow. What made them change their minds? There are many times when we stain different blocks of a single specimen and only one lights up. I, too, would be interested in an official document stating the change. Thanks! Michelle -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Friday, December 30, 2011 3:07 PM To: Martha Ward-Pathology; Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: NCCI policy update Where can I get the article to show my billing dept/pathologists? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Friday, December 30, 2011 1:13 PM To: Webb, Dorothy L; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: NCCI policy update Oh yes, we are very aware and quite upset at the change! Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Friday, December 30, 2011 1:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] NCCI policy update Is everyone aware that beginning 1/1/12, we can no longer bill for each block regarding IHC billing, only one unit of billing for each part type no matter how many blocks are stained? Also IHC cocktail stains, such as PIN4 must now be billed as one unit even though multiple antibodies are reported out. Kind of a surprising reversal of the policy set in motion 10/1/2009. SPECIMEN becomes the unit of service rather than block(s) for IHC codes 88342, 88360, and 88361. Happy New Year to everyone out there. May 2012 find you happiness and health! Dorothy Webb, HT Regions Histology TS 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - No virus found in this message. Checked by AVG - www.avg.com Version: 2012.0.1901 / Virus Database: 2109/4712 - Release Date: 12/30/11 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
