[Histonet] Tuberculosis positive tissue
Hi everyone, was wondering what procedure you have when you find out after the fact that the tissue is positive for TB. What Decontamination procedures do you perform? Also what about documentation? Any help would be appreciated. Thank you Michele Carr Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Tuberculosis positive tissue
If your in a hospital: I've always had it verified with the microbiology department if it hasn't been done already(if not then still the following) Then it needs to be reported to your local department of health and the CDC since it is a reportable disease. I'm sure others can expand on this but this is what I've had to do. Kim Donadio Sent from my iPhone On Jan 20, 2012, at 6:47 AM, Michele Email michelecar...@yahoo.com wrote: Hi everyone, was wondering what procedure you have when you find out after the fact that the tissue is positive for TB. What Decontamination procedures do you perform? Also what about documentation? Any help would be appreciated. Thank you Michele Carr Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Galectin 3 and Tripsin
Hi Histonet, Wondering if anyone out there would be willing to share a company and protocol for Galectin 3 and Trypsin IHC's on paraffin embedded tissue. Thanks in advance. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome Reicher-jung 2030
I am looking to buy 2 of this microtome in very good condition please. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Lab Supervisor College Of Veterinary Medicine Texas AM University Phone (979) 845-3177 Fax (979) 458-3499 lbustama...@cvm.tamu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] K/L bone marrow
-Original Message- Date: Wed, 18 Jan 2012 08:29:48 -0500 From: Clare Thornton cthorn...@dahlchase.com Subject: [Histonet] K/L bone marrow To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: c9d78ffc9d668b4cbea4405f84697504f819b6f...@iris.dahlchase.net Content-Type: text/plain; charset=us-ascii Does anyone have a Kappa/Lambda bone marrow ISH protocol for use on the Ventana Benchmark XT? thanks! Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com -Reply- Hi Clare, Sorry for the delay. Yes, I have a successful Kappa / Lambda ISH protocol for Bone Marrow on the Ventana Benchmark XT. First, the software that you will need to have installed is called XT ISH Open Probes ChromogenicV3. This protocol will use the iView BLUE+ DETECTION and with a RED STAIN II counterstain. Before I get to the protocol, I'll fill you in on the Kappa and Lambda probes. They do NOT arrive in a dispenser. They each arrive in 4 pre-diluted vials, so you will need to fill a user-fillable dispenser. All four pre-diluted vials are mixed together into one user-fillable dispenser. Apparently, Ventana is required to package each separately. Additionally, you will need to run a control. This control will confirm the presence of non-degradated RNA. The control I used was U6, and the protocol is identical to the Kappa or Lambda except for the selected ISH probe. By the way, the selected ISH probe will likely be ISH PROBE 1 or ISH PROBE 2 because the user-fillable dispenser will not have the proper commercial bar code. One last thing, I was concerned initially about Bone Marrow adhesion to the charged slides, so I ran a comparison of 2 leading brands. The clear winner was Superfrost Plus distributed by Cardinal Health. Finally, the following is a successful protocol summary. 1 Baking [Selected] 2 Warmup Slide to [69 Deg C], and Incubate for [20 Minutes] ( Baking ) 3 Deparaffinization [Selected] 4 Standard [Selected] 5 Warmup Slide to [69 Deg C], and Incubate for 4 Minutes ( Deparaffinization ) 6 Enzyme [Selected] 7 Apply Coverslip, One Drop of [ISH-PROTEASE 2] ( Enzyme ), and Incubate for [8 Minutes] 8 Probe [Selected] 9 Probe Auto Dispense [Selected] 10 1 Drop of Probe Dispensed [Selected] 11 Apply One Drop of [ISH Probe 1] ( ISH Probe ), Apply Coverslip, and Incubate for 4 Minutes 12 Denature [Selected] 13 Warmup Slide to [75 Deg C], and Incubate for [4 Minutes] ( Denaturation ) 14 Hybridization [Selected] 15 Warmup Slide to [44 Deg C], and Incubate for 4 Minutes ( Hybridization ) 16 Incubate for [1 Hour] ( Hybridization ) 17 Stringency Washes [Selected] 18 Stringency Wash #1 [Selected] 19 Warmup Slide to [60 Deg C], and Incubate for [8 Minutes] ( Stringency Wash #1 ) 20 Stringency Wash #2 [Selected] 21 Incubate for [8 Minutes] ( Stringency Wash #2 ) 22 Stringency Wash #3 [Selected] 23 Incubate for [8 Minutes] ( Stringency Wash #3 ) 24 Detection Kit [Selected] 25 Blue Detection [Selected] 26 Incubate for [32 Minutes] ( Substrate ) 27 Counterstain [Selected] 28 Apply One Drop of [Red Stain II] ( Counterstain ), Apply Coverslip, and Incubate for [4 Minutes] 29 Post Run LCS Application [Selected] Christopher Lanigan Research Technologist Molecular Pathology Cleveland Clinic Foundation 9500 Euclid Avenue L3-127 Cleveland, OH 44195 === Please consider the environment before printing this e-mail Cleveland Clinic is ranked one of the top hospitals in America by U.S.News World Report (2010). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Correct web address for FSH
Sorry guys, I posted the wrong link o the Florida Society for Histotechnology website. The web address is www.fshgroup.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: slide file storage to dry slides
Yeah. I want this median too. Thanks for heads up Kim Sent from my iPhone On Jan 19, 2012, at 10:25 AM, dkb...@chs.net wrote: Joyce, Interesting! What methodology are using to remove the coverslip and with what difficulty? I may be interested in changing to this medium. Are you using this same medium with Non-gyn Cytology and have you had any bleeding problems? Also we do not use Xylene. We use a substitute. Thanks! Debbie Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Weems, Joyce jwe...@sjha.org Sent by: histonet-boun...@lists.utsouthwestern.edu 01/19/2012 09:58 AM To Sebree Linda A lseb...@uwhealth.org, Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu cc Subject [Histonet] RE: slide file storage to dry slides We use fast dry mounting media from ThermoFisher Scientific - Item# 22 050 102 - that doesn't need extra drying. File the next day with no sticking.. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Thursday, January 19, 2012 09:26 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] slide file storage to dry slides Good morning all, We've recently switched from film coverslipping back to glass and therefore need to thoroughly dry our slides before permanent filing. I recall, in my first histology job30 + years ago, that we used metal stacking slide files that you could put an insert into the drawers that looked like a non-stretchy spring. The wires of this spring held the slides apart to dry, then they could be filed without the spring when they were completely dry. Anyone know if that product still exists? Or does anyone have a better solution for drying slides while still keeping them in order? Thanks for the assist, Linda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] slide file storage to dry slides
I've seen this a couple ways. Metal trays put in slide drying oven on low temp overnight. Low or you will ruin some labels . I've also seen these nice wooden boxes that hold the metal slide trays. You put them in it , it's like a rack . Put them in order. I think they hold about 1000 slides. You just leave these there they will be your most recent. By the time it's full. Put half up and then continue rotating after that. I'd check with fisher maybe Nite nite Kim Sent from my iPhone On Jan 19, 2012, at 9:25 AM, Sebree Linda A lseb...@uwhealth.org wrote: Good morning all, We've recently switched from film coverslipping back to glass and therefore need to thoroughly dry our slides before permanent filing. I recall, in my first histology job30 + years ago, that we used metal stacking slide files that you could put an insert into the drawers that looked like a non-stretchy spring. The wires of this spring held the slides apart to dry, then they could be filed without the spring when they were completely dry. Anyone know if that product still exists? Or does anyone have a better solution for drying slides while still keeping them in order? Thanks for the assist, Linda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
