[Histonet] M1 phenotype marker
Hi Has anyone used anti - CCK7 to differentiate M1 from M2 macrophages and what were your results like? I have a paper and am thinking of getting this antibody but would like to know if anyone else has used anti -CCK7. It will be used on rat tissue. Many thanks Helen Helen Ilsley helen.ils...@uct.ac.za Cardiovascular Research Unit Cape Heart Centre Anzio Road, Observatory, UCT 021-406 6472 021-448 5935 (fax) ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Non-Gyn Cytology Specimen Staining
Good Morning Histonetters, I am looking to speak to someone that can help me with some questions that I have about non-gyn cytology specimen staining. We only do non-gyns in house and we stain I would say about 90% of them separate because of the type of specimen it is. We have been staining the bronchial specimens together and now they are getting cross-contaminated. We do direct smears on our cytology specimens. I am looking to see how other facilities are processing and staining their non-gyn specimens. I have many questions so if you would send me your telephone number and what would be a good time to contact you I will give you a call. Thanks in advance for your help, Amy Amy Self Georgetown Hospital System Georgetown, SC 843-527-7179 (p) NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RISH's Carnival Mardi Gras Conference 3-24-2012
Hi Everyone, The Rhode Island Society for Histotechnology will be hosting their Carnival Mardi Gras Conference on Saturday March 24 2012 at Dave Buster's in the Providence Place Mall, Providence, RI. This is a one day conference providing 7 CEU's. Freida Carson is our guest speaker! To register and for more details please visit RISH's Mardi Gras 2012 page on our website at www.rihisto.org FREE RISH membership is included with conference registration! Laissez les bons temps rouler Nancy Heath, HT (ASCP) President - Rhode Island Society for Histotechnology presid...@rihisto.org phone: 401-444-3246 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology, IHC, Grossing, Cytoprep Job Openings in New York
Allied Search Partners has been retained for the following searches. We have openings in Histology, IHC, Grossing, and Cytoprep. Please forward this along to anyone who you know that would be interested in any of the following positions. We do offer a referral bonus. 1. Please email a copy of updated resume to meli...@alliedsearchpartners.com mailto:bran...@alliedsearchpartners.com for a full job description. We have the following positions available: 1. Immunohistochemistry (IHC) Histotech (2 positions available) LOCATION: Port Chester, NY area DEPARTMENT SCHEDULE: Position #1: Monday-Friday 5pm-1:30am Position #2: Monday-Friday 9pm-5:30am 1. Histotech LOCATION: New York, NY SCHEDULE DEPARTMENT: Monday-Friday 12am (midnight)-8am 1. Cytopreparatory Technician LOCATION: Port Chester, NY area SCHEDULE DEPARTMENT: Monday-Friday 10am-6:30am 1. Grossing Technician LOCATION: Port Chester, NY area SCHEDULE DEPARTMENT Monday-Friday Overnight Shift/3rd shift (exact hours are not determined) Monday-Friday Second Shift (exact hours are not determined) -- Melissa Phelan, President Laboratory Staffing Allied Search Partners http://www.linkedin.com/in/melissaphelan http://www.linkedin.com/in/melissaphelan P: 888-388-7571 F: 888-388-7572 C: 407-697-1175 www.alliedsearchpartners.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Reg: Tissue is hard to cut after processing
Arun, It looks like you are overprocessing your tissues. Uterus and prostate are tissues that are often hard to cut and your schedule for processing may be adding to your difficulties. Unless the pieces are very large and thick (and you can't do anything about that), cut down on the time, eliminate the heat and vacuum in your alcohols and xylenes but not the paraffins. You might also want to eliminate one of the 100% alcohols and one of the xylenes or try a xylene substitute like Clear-Rite 3, which is a more gentle clearing agent. If you eliminated one of these you could add another 70% or 80% alcohol before the 95% to help wash out the formalin. Right now you are really drying out your tissues and making them harder. If your tissues aren't totally fixed you may need the time in Formalin but if they are well fixed you could cut these times also. You probably don't need 4 hrs in paraffin at 63 degrees either. I'd cut the time down and the temp. to at most 60 degrees. I usually go 3-4 degrees above the melting point of the paraffin. Andi Grantham On Jan 25, 2012, at 6:25 AM, Arun Jyothi S.P wrote: Dear all, We are having a hard time in cutting blocks. All our blocks especially uterus, prostate chips etc became very hard after processing, we cant even trim the blocks its very hard. we use SLEE MTM automated tissue processor and our schedule is 1, Formalin- 1hr - ambient - 40 degree 2, Formalin- 2 hr - vacuum - 40 degree 3, 70% ethanol - 1hr - vacuum - Room temp 4, 95% ethanol - 1hr - vacuum - Room temp 5 and 6- 100% ethanol- 1hr each - vacuum - room temp 7, 100% ethanol - 1.30 hr - vacuum - room temp 8, 9 and 10 - Xylene- 1 hr each - vacuum - 30, 35, 40 degree respectively 11,12,13 and 14 - paraffin wax - 1 hr each - vacuum - 63 degree for all. We use Merck paraffin wax of 56 degree M.P Is there any problem with tissue processing schedule or something else is wrong? We are in a big problem really expecting some valuable suggestion and advices. with regards ARUN JYOTHI S.P. Histotechnologist United Laboratories Co. http://www.unitedlabs.com.kw/ Kuwait ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Interview Questions
Okay, My People - I will be one of the interviewers for locating my replacement). I've not been this fortunate before and I do know there are questions one cannot ask so that's not an issue. What I'd like to know is what I SHOULD ask. This position is fairly straightforward - basic veterinary histology with nothing significantly challenging (but with that potential). What would YOU want to know about a candidate that would convince you that this person was The One? I need questions with meat to them. Your suggestions will be much-ly appreciated. Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Interview Questions
I don't have any ideas but I am sorry to hear you are leaving. Good Luck with your future. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara [sbree...@nmda.nmsu.edu] Sent: Wednesday, January 25, 2012 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Interview Questions Okay, My People - I will be one of the interviewers for locating my replacement). I've not been this fortunate before and I do know there are questions one cannot ask so that's not an issue. What I'd like to know is what I SHOULD ask. This position is fairly straightforward - basic veterinary histology with nothing significantly challenging (but with that potential). What would YOU want to know about a candidate that would convince you that this person was The One? I need questions with meat to them. Your suggestions will be much-ly appreciated. Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Interview Questions
I had a great question asked of me when I was interviewed for my current position: Tell me of a time that you had to rescue some tissue; what happened to it that ruined it and what did you do to fix it? A great question for a histologist. People with experience in the field should have several stories related to this question (especially in research, when you have PIs collecting tissue for you that often have no clue what they are doing)!!! Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA From: Burton, Lynn lynn.bur...@illinois.gov To: Breeden, Sara sbree...@nmda.nmsu.edu; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, January 25, 2012 10:42 AM Subject: [Histonet] RE: Interview Questions I don't have any ideas but I am sorry to hear you are leaving. Good Luck with your future. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara [sbree...@nmda.nmsu.edu] Sent: Wednesday, January 25, 2012 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Interview Questions Okay, My People - I will be one of the interviewers for locating my replacement). I've not been this fortunate before and I do know there are questions one cannot ask so that's not an issue. What I'd like to know is what I SHOULD ask. This position is fairly straightforward - basic veterinary histology with nothing significantly challenging (but with that potential). What would YOU want to know about a candidate that would convince you that this person was The One? I need questions with meat to them. Your suggestions will be much-ly appreciated. Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] neutrophil marker for FFPE mouse tissue
Hello! What antibodies are people using to stain for neutrophils in FFPE mouse tissue? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Interview Questions
First of all, DON'T ASSUME ANYTHING. Ask questions about every aspect of the position. Let them know what they will be responsible for. Look for desired qualities ie: detail oriented, high work standard, team worker, flexible, multitasker, critical thinker, acceptable to constructive criticism, good verbal communication, etc. Interview Questions I use are: Are you proficient with frozen sections? Are you willing to work over occasionally to perform frozen sections? What are your interest or hobbies? Where are your professional goals. Where do you see yourself in 5 years. I always ask one critical thinking question about processing to test their knowledge. Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical Center I 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: 804-765-5582 l dkb...@chs.net Breeden, Sara sbree...@nmda.nmsu.edu Sent by: histonet-boun...@lists.utsouthwestern.edu 01/25/2012 10:41 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Interview Questions Okay, My People - I will be one of the interviewers for locating my replacement). I've not been this fortunate before and I do know there are questions one cannot ask so that's not an issue. What I'd like to know is what I SHOULD ask. This position is fairly straightforward - basic veterinary histology with nothing significantly challenging (but with that potential). What would YOU want to know about a candidate that would convince you that this person was The One? I need questions with meat to them. Your suggestions will be much-ly appreciated. Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Interview Questions
Here are some idea starters..http://www.negotiations.com/articles/top-interview-questions/ maybe you can adapt it to your particular job role, the culture and people that you were working with directly, or what you found most challenging/frustrating/inspiring? How interesting to be interviewing your replacement!Joelle Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver Date: Wed, 25 Jan 2012 08:37:29 -0700 From: sbree...@nmda.nmsu.edu To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Interview Questions Okay, My People - I will be one of the interviewers for locating my replacement). I've not been this fortunate before and I do know there are questions one cannot ask so that's not an issue. What I'd like to know is what I SHOULD ask. This position is fairly straightforward - basic veterinary histology with nothing significantly challenging (but with that potential). What would YOU want to know about a candidate that would convince you that this person was The One? I need questions with meat to them. Your suggestions will be much-ly appreciated. Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Antibody for canine IgG
I'm looking for an antibody that works for canine IgG, to be used for FFPE tissue IHC, and which should react with both IgG1 and IgG2. Ideally mouse monoclonal. Does not have to be specific to dog, as long as it reacts with it adequately in IHC. Any recommendations ? _ Jean-Martin Lapointe AccelLAB Inc ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Interview Questions
So far, I am TOTALLY impressed and so grateful for your suggestions. And here's why... did I ever tell anyone out there what the FIRST question I was asked by the pathologist at my interview? It was. (wait for it) How do you feel about personal phone calls?. Un-freakin' believable. I sure don't want someone to remember ME that way!!! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Solutions - Hot Histology Job Alert Here are my newest and most exciting opportunities. Take a look!!
Hi Histonetters!! How are you doing today? I have a few new positions to run by you. These are with some great companies that are offering excellent compensation, benefits and relocation/sign on bonuse. If you are interested contact me at mailto:rel...@earthlink.net rel...@earthlink.net or toll free at 866-607-3542. Here is the list: =Lead Histotech - GI - Kissimmee, FL - Florida license required. This is an in office pathology lab and you would be the sole practitioner. =Night Shift Histology Supervisor - Miami, FL Florida tech or supervisor license is required - opportunity for advancement!! =Histology Tech - Day Shift New York NYS license required. positions in Long Island, Suffern and Rochester. =Histology Tech - Strong Dermpath - Lancaster, PA =Grossing Histotech - Nights, Wallingford, CT =Grossing Histotech - Nights Chattanooga, TN I also need a NY licensed Cytotech for a lab on Long Island - If you know anybody over in the cyto department that might be interested I do pay a referral bonus of $500.00 :) Have a great Hump Day!! Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: mailto:rel...@earthlink.net rel...@earthlink.net http://www.facebook.comPamBarkerRELIA www.facebook.comPamBarkerRELIA http://www.linkedin.com/reliasolutions www.linkedin.com/reliasolutions http://www.myspace.com/pamatrelia www.myspace.com/pamatrelia http://www.twitter.com/pamatrelia www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Oil red O versus Sudan 4
Hi Sarah Actually, There is no micotmy involved. We biopsy the aorta. Clean the surrounding fat, cut it down the middle with a blade. And then we stain with Sudan 4. The aorta is mounted whole on a wax plate. we hydrate the tissue with PBS before we stain. This worked fine with the Sudan 4. Should I discontinue hydrating with PBS and use water instead with the Oil Red O. Also with the Oil red O, we were rinsing them with isopranol before we stained them. Could this be the problem? I remain yours truely, Candice Camille From: Sarah Dysart sdys...@mirnarx.com To: Candice Smoots candice_cami...@yahoo.com Sent: Wednesday, January 25, 2012 10:32 AM Subject: RE: [Histonet] Oil red O versus Sudan 4 I do oil red a lot. You are doing it on frozen sections right? What are you coverslipping with? Need a few more details of the protocol and I might be able to help =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Candice Smoots Sent: Wednesday, January 25, 2012 10:21 AM To: Histonet Subject: [Histonet] Oil red O versus Sudan 4 Hi Histonetters I am staining the plaques in aorta. I perfuse my animal with pbs before I biospy the aorta. I then pin the aorta down onto a wax plate and then i stain the inside of the aorta for my plaques. This seems to work well with the Sudan 4 but not so much with the Oil red o. The person who was doing the sudan 4 is no longer here and we have plenty of Oil red o. My thinking was that it should stain the same but I am not getting results with the Oil red o. My question is what is the difference and what should I do differently with the Oil red o? Thanks so much for your help! I remain yours truely, Candice Camille ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Oil red O versus Sudan 4
That would be great! Thanks I remain yours truely, Candice Camille From: Elizabeth Chlipala l...@premierlab.com To: 'Candice Smoots' candice_cami...@yahoo.com; Histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, January 25, 2012 10:43 AM Subject: RE: [Histonet] Oil red O versus Sudan 4 Candice Most of the references regarding this technique use Sudan IV, that's what we use here. We use Oil Red O for the Aortic Root Sections but not for the En Face Analysis of the Aorta. I have the Sudan IV procedure we use here if you would like it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Candice Smoots Sent: Wednesday, January 25, 2012 9:21 AM To: Histonet Subject: [Histonet] Oil red O versus Sudan 4 Hi Histonetters I am staining the plaques in aorta. I perfuse my animal with pbs before I biospy the aorta. I then pin the aorta down onto a wax plate and then i stain the inside of the aorta for my plaques. This seems to work well with the Sudan 4 but not so much with the Oil red o. The person who was doing the sudan 4 is no longer here and we have plenty of Oil red o. My thinking was that it should stain the same but I am not getting results with the Oil red o. My question is what is the difference and what should I do differently with the Oil red o? Thanks so much for your help! I remain yours truely, Candice Camille ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Interview Questions
Hi Sally, I think I would ask if they were familiar with the terms bovine, porcine, ovine, caprine, equine, ect. Then ask if they would process bovine tissue differently than mouse tissues. Ask what kind of problems they might expect to encounter doing IHC and immunofluorescence on animal tissue. If you handle legal cases, ask questions about tissue identity and chain of evidence. Ask questions on lab safety and chemical waste disposal. Good luck, Tom Truscott -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, January 25, 2012 7:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Interview Questions Okay, My People - I will be one of the interviewers for locating my replacement). I've not been this fortunate before and I do know there are questions one cannot ask so that's not an issue. What I'd like to know is what I SHOULD ask. This position is fairly straightforward - basic veterinary histology with nothing significantly challenging (but with that potential). What would YOU want to know about a candidate that would convince you that this person was The One? I need questions with meat to them. Your suggestions will be much-ly appreciated. Gracias! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Interview Questions
It would seem that questions like How do you feel about cannibalism? might also be out but might be far more helpful; than phone questions. On the serious side, when I was much younger I hired a person who was able to answer all the right histo questions and so I hired him. He turned out to be a poser, who, shortly after I fired him showed up at a local university with a lab coat that listed him as Dr. He had indeed worked in a histo lab, but as a lab assistant, and so the the understanding of what a histologist does was well rehearsed. (BTW, it topok me about two weeks to catch on, though the more experienced techs in the department figured it out almost right away) To be fair, it was during a time in hiring history when HR departments were not willing to give useful reference data and there were only a handful of questions they would even ask when checking. None of them were particularly useful or telling. For inistance, they would not ask if the person was an histo tech, but would simply ask, did he indeed work at your institution? The place where I worked required little or nothing for proof of experience. There was no background check either. Today, however, reference checking is a lot easier and more reliable. I guess my point here is that a good reference check needs to be done as well weeding them out by histo questions. I'm sure your HR folks will do a fine job of this. Also, once you have determined that they actually have the skills, or a realistic potential of gaining them, questions concerning dynamics of interaction are appropriate, though may lead to wrong impressions in the mind of the applicant. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, January 25, 2012 10:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Interview Questions So far, I am TOTALLY impressed and so grateful for your suggestions. And here's why... did I ever tell anyone out there what the FIRST question I was asked by the pathologist at my interview? It was. (wait for it) How do you feel about personal phone calls?. Un-freakin' believable. I sure don't want someone to remember ME that way!!! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Interview Questions
Good point about personality questions. I have often had this experience, where I was leaving the meeting wondering about the place from too much time spent on this sort of thing. However, I think that some line of questioning for this information is good to try to see if you can learn a little about everyone's general temperment - though I do concede this is difficult in such a staged interaction as an interview. Sometimes people have knowledge and technical skills, but are very confrontational, poor communicators, or have other attributes which make them a bad fit for any particular organization, and sometimes these things end up sinking the ship so to speak as far as the employee-employer relationship, even when skills, reference or credentials are there. Everything about an interview is pretty much a calculated risk I suppose. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver Date: Wed, 25 Jan 2012 10:19:05 -0700 From: billodonn...@catholichealth.net To: sbree...@nmda.nmsu.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Interview Questions CC: It would seem that questions like How do you feel about cannibalism? might also be out but might be far more helpful; than phone questions. On the serious side, when I was much younger I hired a person who was able to answer all the right histo questions and so I hired him. He turned out to be a poser, who, shortly after I fired him showed up at a local university with a lab coat that listed him as Dr. He had indeed worked in a histo lab, but as a lab assistant, and so the the understanding of what a histologist does was well rehearsed. (BTW, it topok me about two weeks to catch on, though the more experienced techs in the department figured it out almost right away) To be fair, it was during a time in hiring history when HR departments were not willing to give useful reference data and there were only a handful of questions they would even ask when checking. None of them were particularly useful or telling. For inistance, they would not ask if the person was an histo tech, but would simply ask, did he indeed work at your institution? The place where I worked required little or nothing for proof of experience. There was no background check either. Today, however, reference checking is a lot easier and more reliable. I guess my point here is that a good reference check needs to be done as well weeding them out by histo questions. I'm sure your HR folks will do a fine job of this. Also, once you have determined that they actually have the skills, or a realistic potential of gaining them, questions concerning dynamics of interaction are appropriate, though may lead to wrong impressions in the mind of the applicant. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, January 25, 2012 10:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Interview Questions So far, I am TOTALLY impressed and so grateful for your suggestions. And here's why... did I ever tell anyone out there what the FIRST question I was asked by the pathologist at my interview? It was. (wait for it) How do you feel about personal phone calls?. Un-freakin' believable. I sure don't want someone to remember ME that way!!! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Interview Questions
If your replacement will be doing actual histology, will your institution permit the applicant to embed and cut? Can you sit down at a multi-head scope and review slides with them? What will the person be responsible for? Do they have experience with all of these tasks? What would they do in a crisis situation (you can make up one yourself that would be plausible). People who volunteer in their personal lives, may do the same at work. Ask how they juggle their schedule though, if there is a lot going on in their personal lives. Be careful with how you ask these questions though. Your HR department should be able to give you guidance in how to phrase things. Good luck. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, January 25, 2012 12:19 PM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Interview Questions It would seem that questions like How do you feel about cannibalism? might also be out but might be far more helpful; than phone questions. On the serious side, when I was much younger I hired a person who was able to answer all the right histo questions and so I hired him. He turned out to be a poser, who, shortly after I fired him showed up at a local university with a lab coat that listed him as Dr. He had indeed worked in a histo lab, but as a lab assistant, and so the the understanding of what a histologist does was well rehearsed. (BTW, it topok me about two weeks to catch on, though the more experienced techs in the department figured it out almost right away) To be fair, it was during a time in hiring history when HR departments were not willing to give useful reference data and there were only a handful of questions they would even ask when checking. None of them were particularly useful or telling. For inistance, they would not ask if the person was an histo tech, but would simply ask, did he indeed work at your institution? The place where I worked required little or nothing for proof of experience. There was no background check either. Today, however, reference checking is a lot easier and more reliable. I guess my point here is that a good reference check needs to be done as well weeding them out by histo questions. I'm sure your HR folks will do a fine job of this. Also, once you have determined that they actually have the skills, or a realistic potential of gaining them, questions concerning dynamics of interaction are appropriate, though may lead to wrong impressions in the mind of the applicant. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, January 25, 2012 10:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Interview Questions So far, I am TOTALLY impressed and so grateful for your suggestions. And here's why... did I ever tell anyone out there what the FIRST question I was asked by the pathologist at my interview? It was. (wait for it) How do you feel about personal phone calls?. Un-freakin' believable. I sure don't want someone to remember ME that way!!! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical
RE: [Histonet] Interview Questions
How did you answer?! Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver Date: Wed, 25 Jan 2012 09:52:08 -0700 From: sbree...@nmda.nmsu.edu To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Interview Questions So far, I am TOTALLY impressed and so grateful for your suggestions. And here's why... did I ever tell anyone out there what the FIRST question I was asked by the pathologist at my interview? It was. (wait for it) How do you feel about personal phone calls?. Un-freakin' believable. I sure don't want someone to remember ME that way!!! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Interview Questions
Love this! I always want to do demonstration during technical interviews, but usually get shot down from managers and argued with in general, as in people don't feel that they should have to prove they can do histology. This perception, I never got, because I always saw it as in a job interview-in what other situation are you more trying to prove or impress with your knowledge, attitude, skills and experience? If you do bench work, you can tell in just a few minutes of observation much more information than you could get with quite a few questions. To be fair, I take into account nervousness, being closely observed, and lack of familiarity with equipment etc. I don't know, I think its fair if those are important skills to the position/role. Was not sure if Sara's job was mostly technical though, so thought I might keep it general. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver From: trathbo...@somerset-healthcare.com To: billodonn...@catholichealth.net; sbree...@nmda.nmsu.edu; histonet@lists.utsouthwestern.edu Date: Wed, 25 Jan 2012 17:47:01 + Subject: RE: [Histonet] Interview Questions CC: If your replacement will be doing actual histology, will your institution permit the applicant to embed and cut? Can you sit down at a multi-head scope and review slides with them? What will the person be responsible for? Do they have experience with all of these tasks? What would they do in a crisis situation (you can make up one yourself that would be plausible). People who volunteer in their personal lives, may do the same at work. Ask how they juggle their schedule though, if there is a lot going on in their personal lives. Be careful with how you ask these questions though. Your HR department should be able to give you guidance in how to phrase things. Good luck. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, January 25, 2012 12:19 PM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Interview Questions It would seem that questions like How do you feel about cannibalism? might also be out but might be far more helpful; than phone questions. On the serious side, when I was much younger I hired a person who was able to answer all the right histo questions and so I hired him. He turned out to be a poser, who, shortly after I fired him showed up at a local university with a lab coat that listed him as Dr. He had indeed worked in a histo lab, but as a lab assistant, and so the the understanding of what a histologist does was well rehearsed. (BTW, it topok me about two weeks to catch on, though the more experienced techs in the department figured it out almost right away) To be fair, it was during a time in hiring history when HR departments were not willing to give useful reference data and there were only a handful of questions they would even ask when checking. None of them were particularly useful or telling. For inistance, they would not ask if the person was an histo tech, but would simply ask, did he indeed work at your institution? The place where I worked required little or nothing for proof of experience. There was no background check either. Today, however, reference checking is a lot easier and more reliable. I guess my point here is that a good reference check needs to be done as well weeding them out by histo questions. I'm sure your HR folks will do a fine job of this. Also, once you have determined that they actually have the skills, or a realistic potential of gaining them, questions concerning dynamics of interaction are appropriate, though may lead to wrong impressions in the mind of the applicant. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, January 25, 2012 10:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Interview Questions So far, I am TOTALLY impressed and so grateful for your suggestions. And here's why... did I ever tell anyone out there what the FIRST question I was asked by the pathologist at my interview? It was. (wait for it) How do you feel about personal phone calls?. Un-freakin' believable. I sure don't want someone to remember ME that way!!! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud NE Albuquerque, NM 87102 505-383-9278 (Histology Lab) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain
RE: [Histonet] Interview Questions
I guess someone could get hurt. I had to stop someone once, either they were unbelievably nervous, or had not used a microtome in QUITE some time, and I thought they might hurt themselves. I stopped the activity, but legalities might prevail, a consent could suffice to cover for this maybe. Probation is good, if it is enforced. Have seen people that I was not sure how they made it through that period, so I guess the weight that is given varies. All in all, a challenge to locate, recruit, screen, hire and retain good people! Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver From: powell...@mercer.edu To: louise.ren...@gmail.com Date: Wed, 25 Jan 2012 13:15:48 -0500 Subject: RE: [Histonet] Interview Questions CC: histonet@lists.utsouthwestern.edu Make them sign a non-liability clause before doing the test? You need to know if they can do the work before hiring, not after, nervous or not, and not how well they answer questions. sp -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Louise Renton Sent: Wednesday, January 25, 2012 1:09 PM To: Histonet Subject: Re: [Histonet] Interview Questions Just to be devil's advocate here.. asking a person to prove their skills - what happens, if through nervousness, or being unfamiliar with the equipment, they injure themselves. Where does the liability lie? Rather ask questions regarding cutting speed, way in which tissue is embedded etc, and review the person's skill during an agreed probationary period. If they are not what u expected, then you can get rid of them. ... On Wed, Jan 25, 2012 at 8:02 PM, joelle weaver joellewea...@hotmail.comwrote: Love this! I always want to do demonstration during technical interviews, but usually get shot down from managers and argued with in general, as in people don't feel that they should have to prove they can do histology. This perception, I never got, because I always saw it as in a job interview-in what other situation are you more trying to prove or impress with your knowledge, attitude, skills and experience? If you do bench work, you can tell in just a few minutes of observation much more information than you could get with quite a few questions. To be fair, I take into account nervousness, being closely observed, and lack of familiarity with equipment etc. I don't know, I think its fair if those are important skills to the position/role. Was not sure if Sara's job was mostly technical though, so thought I might keep it general. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver From: trathbo...@somerset-healthcare.com To: billodonn...@catholichealth.net; sbree...@nmda.nmsu.edu; histonet@lists.utsouthwestern.edu Date: Wed, 25 Jan 2012 17:47:01 + Subject: RE: [Histonet] Interview Questions CC: If your replacement will be doing actual histology, will your institution permit the applicant to embed and cut? Can you sit down at a multi-head scope and review slides with them? What will the person be responsible for? Do they have experience with all of these tasks? What would they do in a crisis situation (you can make up one yourself that would be plausible). People who volunteer in their personal lives, may do the same at work. Ask how they juggle their schedule though, if there is a lot going on in their personal lives. Be careful with how you ask these questions though. Your HR department should be able to give you guidance in how to phrase things. Good luck. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, January 25, 2012 12:19 PM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Interview Questions It would seem that questions like How do you feel about cannibalism? might also be out but might be far more helpful; than phone questions. On the serious side, when I was much younger I hired a person who was able to answer all the right histo questions and so I hired him. He turned out to be a poser, who, shortly after I fired him showed up at a local university with a lab coat that listed him as Dr. He had indeed worked in a histo lab, but as a lab assistant, and so the the understanding of what a histologist does was well rehearsed. (BTW, it topok me about two weeks to catch on, though the more experienced techs in the department figured it out almost right away) To be fair, it was during a time in hiring history when HR departments were not willing to give useful reference data and there were only a handful of questions they would even ask when checking. None of them were particularly useful or telling. For inistance, they would not
[Histonet] Re: Interview questions
Sally, My answer to that question is I *love* personal phone calls! I just think they are inappropriate in a workplace and should be minimized whenever possible. Do not ask yes or no questions. Keep them open ended. Don't ignore red flags that arise, even if you can't explain why it's a cause for concern. Sometimes you just have to go with your gut. Ask them what they think they can contribute that is unique from the other candidates you are interviewing. Ask them to describe their perfect job. You can get a lot of information from those two questions, more even than the answers at face value. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H Pylori Control
Good Afternoon everyone, My institution is about to start running H Pylori for another lab. They microwave process their tissue. Would there be anyone out there who microwave processes and would have one or two blocks of H Pylori positive control tissue they would be willing to let us have? Thanks in advance, Rae Ann Staskiewicz Methodist Medical Center of Illinois Peoria, IL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
http://lizziethealienchild.com/wp-content/plugins/extended-comment-options/tndkrno.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Xylene and Preggers
Human resources ultimately represents the company . A pregnant employee should be wearing ppe when handling xylene. MSDS forms are accessible to all employees, just a click away on the computer. I can understand wanting to keep some things private until you are ready to communicate them, but when you work with chemicals such as xylene and you are pregnant making sure you are wearing the proper PPE and the air quality meets OSHA standards comes first. I don't think the information communicated by HR to your supervisors should become common knowledge, but there is an obligation to protect the employee as well as the employer, Rena Fail On Mon, Jan 23, 2012 at 12:26 PM, Sarah Dysart sdys...@mirnarx.com wrote: Hey all, so 2 things... A. Does anyone have anything saying that .75% (yes less than 1) is an acceptable exposure limit for a pregnant person and B. Does HR have the right to tell people that you are pregnant after you ask them questions (ie. Your manager, and all the way up??) Thanks Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Interview Questions
Your company will have to invest a lot of money to hire the person you choose. Background check and physical to start with. Then a training period. If you could have known during practical session that the applicant would not measure up to the needs of the department, you will save yourself time (for training), and HR (financial). Also, if the person gave up a job to take yours, and was terminated after the probationary period, that leaves them without a job too. Not a good scenario for either side. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Wednesday, January 25, 2012 1:16 PM To: Louise Renton Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Interview Questions Make them sign a non-liability clause before doing the test? You need to know if they can do the work before hiring, not after, nervous or not, and not how well they answer questions. sp -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Louise Renton Sent: Wednesday, January 25, 2012 1:09 PM To: Histonet Subject: Re: [Histonet] Interview Questions Just to be devil's advocate here.. asking a person to prove their skills - what happens, if through nervousness, or being unfamiliar with the equipment, they injure themselves. Where does the liability lie? Rather ask questions regarding cutting speed, way in which tissue is embedded etc, and review the person's skill during an agreed probationary period. If they are not what u expected, then you can get rid of them. ... On Wed, Jan 25, 2012 at 8:02 PM, joelle weaver joellewea...@hotmail.comwrote: Love this! I always want to do demonstration during technical interviews, but usually get shot down from managers and argued with in general, as in people don't feel that they should have to prove they can do histology. This perception, I never got, because I always saw it as in a job interview-in what other situation are you more trying to prove or impress with your knowledge, attitude, skills and experience? If you do bench work, you can tell in just a few minutes of observation much more information than you could get with quite a few questions. To be fair, I take into account nervousness, being closely observed, and lack of familiarity with equipment etc. I don't know, I think its fair if those are important skills to the position/role. Was not sure if Sara's job was mostly technical though, so thought I might keep it general. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver From: trathbo...@somerset-healthcare.com To: billodonn...@catholichealth.net; sbree...@nmda.nmsu.edu; histonet@lists.utsouthwestern.edu Date: Wed, 25 Jan 2012 17:47:01 + Subject: RE: [Histonet] Interview Questions CC: If your replacement will be doing actual histology, will your institution permit the applicant to embed and cut? Can you sit down at a multi-head scope and review slides with them? What will the person be responsible for? Do they have experience with all of these tasks? What would they do in a crisis situation (you can make up one yourself that would be plausible). People who volunteer in their personal lives, may do the same at work. Ask how they juggle their schedule though, if there is a lot going on in their personal lives. Be careful with how you ask these questions though. Your HR department should be able to give you guidance in how to phrase things. Good luck. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, January 25, 2012 12:19 PM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Interview Questions It would seem that questions like How do you feel about cannibalism? might also be out but might be far more helpful; than phone questions. On the serious side, when I was much younger I hired a person who was able to answer all the right histo questions and so I hired him. He turned out to be a poser, who, shortly after I fired him showed up at a local university with a lab coat that listed him as Dr. He had indeed worked in a histo lab, but as a lab assistant, and so the the understanding of what a histologist does was well rehearsed. (BTW, it topok me about two weeks to catch on, though the more experienced techs in the department figured it out almost right away) To be fair, it was during a time in hiring history when HR departments were not willing to give useful reference data and there were only a handful of questions they would even ask when checking. None of them were particularly useful or telling. For inistance, they would not ask if the person was an
[Histonet] peroxidase blocking in whole tissues
Hello histonet, I am working on a whole-mount Pecam immunostain on mouse embryo hearts. During the peroxidase blocking step (1% H2O2 in PBS) there was visible vigorous bubbling in the tissue which caused the heart to swell up. I will try a lower concentration of H2O2 and also prepare the solution in MeOH which I have read is more gentle on tissues with high endogenous peroxidase activity. My questions are 1) Why is MeOH more gentle than H2O2 in aqueous solutions? and 2) Is there anyone out there in histoland who has done this experiment and can recommend a protocol? Thank you, Andrea Marion Graduate Student University of Illinois at Chicago ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] overfixation of GI biopsies?
Hi to all- I am looking through documentation and some say there is no such thing as overfixation - some say there is . Does overfixation only pertain to IHC? We are seeing some trouble with GI biopsies on Mon. morn - this is after they have been in formalin for 24 hours - any GI specialists out there? Thanks so much Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 98, Issue 32
Also, to help cut the tissues, I face at room temperature (very slowly), then I place my uterus/prostate blocks on ice with lots of water, then I cut them last. This helps to allow the blocks to soak up the water and soften some, plus allows nice thin sections. I do agree with Andi that the processing procedure is too long with too many 100% alcohols. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org -- Message: 16 Date: Wed, 25 Jan 2012 07:22:07 -0800 From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu Subject: Re: [Histonet] Reg: Tissue is hard to cut after processing Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: d5cbd3e4-5447-4f24-964c-19bf8144e...@email.arizona.edu Content-Type: text/plain; charset=us-ascii Arun, It looks like you are overprocessing your tissues. Uterus and prostate are tissues that are often hard to cut and your schedule for processing may be adding to your difficulties. Unless the pieces are very large and thick (and you can't do anything about that), cut down on the time, eliminate the heat and vacuum in your alcohols and xylenes but not the paraffins. You might also want to eliminate one of the 100% alcohols and one of the xylenes or try a xylene substitute like Clear-Rite 3, which is a more gentle clearing agent. If you eliminated one of these you could add another 70% or 80% alcohol before the 95% to help wash out the formalin. Right now you are really drying out your tissues and making them harder. If your tissues aren't totally fixed you may need the time in Formalin but if they are well fixed you could cut these times also. You probably don't need 4 hrs in paraffin at 63 degrees either. I'd cut the time down and the temp. to at most 60 degrees. I usually go 3-4 degrees above the melting point of the paraffin. Andi Grantham On Jan 25, 2012, at 6:25 AM, Arun Jyothi S.P wrote: Dear all, We are having a hard time in cutting blocks. All our blocks especially uterus, prostate chips etc became very hard after processing, we cant even trim the blocks its very hard. we use SLEE MTM automated tissue processor and our schedule is 1, Formalin- 1hr - ambient - 40 degree 2, Formalin- 2 hr - vacuum - 40 degree 3, 70% ethanol - 1hr - vacuum - Room temp 4, 95% ethanol - 1hr - vacuum - Room temp 5 and 6- 100% ethanol- 1hr each - vacuum - room temp 7, 100% ethanol - 1.30 hr - vacuum - room temp 8, 9 and 10 - Xylene- 1 hr each - vacuum - 30, 35, 40 degree respectively 11,12,13 and 14 - paraffin wax - 1 hr each - vacuum - 63 degree for all. We use Merck paraffin wax of 56 degree M.P Is there any problem with tissue processing schedule or something else is wrong? We are in a big problem really expecting some valuable suggestion and advices. with regards ARUN JYOTHI S.P. Histotechnologist United Laboratories Co. http://www.unitedlabs.com.kw/ Kuwait ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] interview questions
I think it is great that you are using the histonet this way to ask probing questions. SInce they are replacing you, I would get a feel for how they would do your job by telling them the general taskings and letting them tell you what they know about all of the taskings. I have also found that some people can really answer questions well but then when they are asked to perform, there seems to be some, um confusement. Yes confusement, my own werd. Seriously if I interview anyone again, I will see if I can ask them to mock going through some of the taks, like without an actual blade or sample mock setting up to cut, embed, stain, maybe even some simple computer tasks. ANy chance you can overlap with the replacement? Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8196 pat...@gwumc.edu BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:pat...@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] oct to paraffin
If you can spare some nbf the best thing to do would be to place the oct block right into NBF and use that as the wash and then move onto a fresh change of NBF. I would avoid straight water. NBF has enough water in it to rinse off the NBF, 1 or 2 changee for 15 minutes each is more than enough and a safe bet. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8196 pat...@gwumc.edu BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:pat...@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Signal amplification
What kits/vendors are people using for their CSA/tyramide amplification protocols? Thanks in advance! Andrea On Jan 25, 2012, at 3:45 PM, Mayer,Toysha N tnma...@mdanderson.org wrote: Also, to help cut the tissues, I face at room temperature (very slowly), then I place my uterus/prostate blocks on ice with lots of water, then I cut them last. This helps to allow the blocks to soak up the water and soften some, plus allows nice thin sections. I do agree with Andi that the processing procedure is too long with too many 100% alcohols. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org -- Message: 16 Date: Wed, 25 Jan 2012 07:22:07 -0800 From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu Subject: Re: [Histonet] Reg: Tissue is hard to cut after processing Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: d5cbd3e4-5447-4f24-964c-19bf8144e...@email.arizona.edu Content-Type: text/plain; charset=us-ascii Arun, It looks like you are overprocessing your tissues. Uterus and prostate are tissues that are often hard to cut and your schedule for processing may be adding to your difficulties. Unless the pieces are very large and thick (and you can't do anything about that), cut down on the time, eliminate the heat and vacuum in your alcohols and xylenes but not the paraffins. You might also want to eliminate one of the 100% alcohols and one of the xylenes or try a xylene substitute like Clear-Rite 3, which is a more gentle clearing agent. If you eliminated one of these you could add another 70% or 80% alcohol before the 95% to help wash out the formalin. Right now you are really drying out your tissues and making them harder. If your tissues aren't totally fixed you may need the time in Formalin but if they are well fixed you could cut these times also. You probably don't need 4 hrs in paraffin at 63 degrees either. I'd cut the time down and the temp. to at most 60 degrees. I usually go 3-4 degrees above the melting point of the paraffin. Andi Grantham On Jan 25, 2012, at 6:25 AM, Arun Jyothi S.P wrote: Dear all, We are having a hard time in cutting blocks. All our blocks especially uterus, prostate chips etc became very hard after processing, we cant even trim the blocks its very hard. we use SLEE MTM automated tissue processor and our schedule is 1, Formalin- 1hr - ambient - 40 degree 2, Formalin- 2 hr - vacuum - 40 degree 3, 70% ethanol - 1hr - vacuum - Room temp 4, 95% ethanol - 1hr - vacuum - Room temp 5 and 6- 100% ethanol- 1hr each - vacuum - room temp 7, 100% ethanol - 1.30 hr - vacuum - room temp 8, 9 and 10 - Xylene- 1 hr each - vacuum - 30, 35, 40 degree respectively 11,12,13 and 14 - paraffin wax - 1 hr each - vacuum - 63 degree for all. We use Merck paraffin wax of 56 degree M.P Is there any problem with tissue processing schedule or something else is wrong? We are in a big problem really expecting some valuable suggestion and advices. with regards ARUN JYOTHI S.P. Histotechnologist United Laboratories Co. http://www.unitedlabs.com.kw/ Kuwait ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Interview Questions
I used to give a 10 question test on general histology. I also had the expected answers written down and on my copy. Was accused once of being a racist. What saved me was having the answers in front of me. The person didn't get one answer correct. I had a couple of embedding questions, some cutting, special stains, immunos and some QC questions. I gave the interviewee the test while I was reviewing their resume. I would also see what their facial expressions were too. I had one person tell me they didn't do specials or immunos and didn't like embedding either. When I asked if they liked filing blocks and slides, they really would rather have a lab aide do it. This person didn't have to finish the test. Too make matters worse, she wore a denim miniskirt to boot. Just my three cents Joe - Original Message - From: joelle weaver joellewea...@hotmail.com To: trathbo...@somerset-healthcare.com; billodonn...@catholichealth.net; sbree...@nmda.nmsu.edu; Histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, January 25, 2012 12:02 PM Subject: RE: [Histonet] Interview Questions Love this! I always want to do demonstration during technical interviews, but usually get shot down from managers and argued with in general, as in people don't feel that they should have to prove they can do histology. This perception, I never got, because I always saw it as in a job interview-in what other situation are you more trying to prove or impress with your knowledge, attitude, skills and experience? If you do bench work, you can tell in just a few minutes of observation much more information than you could get with quite a few questions. To be fair, I take into account nervousness, being closely observed, and lack of familiarity with equipment etc. I don't know, I think its fair if those are important skills to the position/role. Was not sure if Sara's job was mostly technical though, so thought I might keep it general. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver From: trathbo...@somerset-healthcare.com To: billodonn...@catholichealth.net; sbree...@nmda.nmsu.edu; histonet@lists.utsouthwestern.edu Date: Wed, 25 Jan 2012 17:47:01 + Subject: RE: [Histonet] Interview Questions CC: If your replacement will be doing actual histology, will your institution permit the applicant to embed and cut? Can you sit down at a multi-head scope and review slides with them? What will the person be responsible for? Do they have experience with all of these tasks? What would they do in a crisis situation (you can make up one yourself that would be plausible). People who volunteer in their personal lives, may do the same at work. Ask how they juggle their schedule though, if there is a lot going on in their personal lives. Be careful with how you ask these questions though. Your HR department should be able to give you guidance in how to phrase things. Good luck. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, January 25, 2012 12:19 PM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Interview Questions It would seem that questions like How do you feel about cannibalism? might also be out but might be far more helpful; than phone questions. On the serious side, when I was much younger I hired a person who was able to answer all the right histo questions and so I hired him. He turned out to be a poser, who, shortly after I fired him showed up at a local university with a lab coat that listed him as Dr. He had indeed worked in a histo lab, but as a lab assistant, and so the the understanding of what a histologist does was well rehearsed. (BTW, it topok me about two weeks to catch on, though the more experienced techs in the department figured it out almost right away) To be fair, it was during a time in hiring history when HR departments were not willing to give useful reference data and there were only a handful of questions they would even ask when checking. None of them were particularly useful or telling. For inistance, they would not ask if the person was an histo tech, but would simply ask, did he indeed work at your institution? The place where I worked required little or nothing for proof of experience. There was no background check either. Today, however, reference checking is a lot easier and more reliable. I guess my point here is that a good reference check needs to be done as well weeding them out by histo questions. I'm sure your HR folks will do a fine job of this. Also, once you have determined that they actually have the skills, or a realistic potential of gaining them, questions concerning dynamics of interaction are appropriate, though may lead to wrong impressions in the mind of the applicant. -Original Message- From:
Re: [Histonet] Interview Questions
A long time ago, I worked in a lab where we had a manual dexterity test that we gave to all applicants for medical technologist or histotechnologist positions. It was designed by psychologists to test hand-eye coordination, spatial orientation, fine motor skills, and (to a certain degree) reasoning skills. Our HR department also signed off on use of the test. We found that doing well on the test did not predict an employee with good skills, but doing poorly on the test pointed out those who would never be able to cope in our laboratory. I'll dig through some old files and see if I can find more info on this test. Two questions I have always asked: 1. Describe the characteristics of the best supervisor/manager for whom you have worked. 2. Describe the characteristics of the worst supervisor/manager for whom you have worked. (For new graduates, I substitute professor for manager.) One applicant, who seemed to have all of the technical skills, described his worst manager as one who sounded just like me: hands-on, involved in day-to-day operations of the lab, picky about being to work on time, perfectionist. I knew right then that we would not be a fit. Good luck with your search! Eric Hoy === Eric S. Hoy, Ph.D., SI(ASCP) Clinical Associate Professor Department of Medical Laboratory Sciences The University of Texas Southwestern Medical Center Dallas, Texas Email: eric@utsouthwestern.edu === On 1/25/12 12:09 PM, Louise Renton louise.ren...@gmail.com wrote: Just to be devil's advocate here.. asking a person to prove their skills - what happens, if through nervousness, or being unfamiliar with the equipment, they injure themselves. Where does the liability lie? Rather ask questions regarding cutting speed, way in which tissue is embedded etc, and review the person's skill during an agreed probationary period. If they are not what u expected, then you can get rid of them. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Interview Questions
This is certainly an interesting thread and I generally hate to get into these ever but I still can't figure out one thing and never have over all these years in pathology. What other endeavor in life and job seeking is an on-the-spot demo that you can do something required at a job interview? Does a lawyer have to go into a courtroom for 5 minutes and show he/she can say I object? Does a sanitation worker have to go round the block once and show he/she can empty 9 cans in 5 minutes? Does a doctor need to show he/she can use a stethoscope? Does a bricklayer have to show he/she can lay 20 bricks in 2 minutes? Or fail the interview? Does a med tech have to show they can stain 6 tubes with CD4 and CD 8 and successfully put them on a flow cytometer? Does an actuary have to show they can really add 100 4-digit numbers on a calculator without a mistake? Does a grocery bagger boy /girl have to show they can put x number of items in 3 bags? Does a Pathologist have to show they know how to turn on a microscope and look through it? Does a peanut counter have to show they can count peanuts? I just can't get into my mind the necessity of someone having to cut to show they can cut? What other profession does this at an interview? Now certainly you can come up with scenarios where it might be important to find out. A brand new histotech whose only cut 3 blocks in their life. A tech from the deepest, darkest nether regions of the earth where you cannot check on their background. But a tech whose has been working cutting the last 3 or 7 or 15 years and you've verified with a previous company that is exactly what they did; how will them cutting for 10 minutes further stratify them into yes or no categories. If 2 potential techs cut and one finishes in 9 minutes and one in 10 minutes, is that a true qualifier or disqualifier of what they can do cutting? There are a myriad of things I'd love to know and always ask; personality, job knowledge, wants, desires, needs, ambitions, etc, etc, etc. My blood pressure skyrockets when I give blood because I HATE anyone sticking a needle in me. But I have a really needed blood type. Should nervousness each time disqualify me. This still boggles my mind about what is being accomplished with cutting during an interview? Ray Seattle, WA - Original Message - From: joelle weaver joellewea...@hotmail.com To: trathbo...@somerset-healthcare.com, billodonn...@catholichealth.net, sbree...@nmda.nmsu.edu, Histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, January 25, 2012 10:02:39 AM Subject: RE: [Histonet] Interview Questions Love this! I always want to do demonstration during technical interviews, but usually get shot down from managers and argued with in general, as in people don't feel that they should have to prove they can do histology. This perception, I never got, because I always saw it as in a job interview-in what other situation are you more trying to prove or impress with your knowledge, attitude, skills and experience? If you do bench work, you can tell in just a few minutes of observation much more information than you could get with quite a few questions. To be fair, I take into account nervousness, being closely observed, and lack of familiarity with equipment etc. I don't know, I think its fair if those are important skills to the position/role. Was not sure if Sara's job was mostly technical though, so thought I might keep it general. Joelle Weaver MAOM, (HTL) ASCP http://www.linkedin.com/in/joelleweaver From: trathbo...@somerset-healthcare.com To: billodonn...@catholichealth.net; sbree...@nmda.nmsu.edu; histonet@lists.utsouthwestern.edu Date: Wed, 25 Jan 2012 17:47:01 + Subject: RE: [Histonet] Interview Questions CC: If your replacement will be doing actual histology, will your institution permit the applicant to embed and cut? Can you sit down at a multi-head scope and review slides with them? What will the person be responsible for? Do they have experience with all of these tasks? What would they do in a crisis situation (you can make up one yourself that would be plausible). People who volunteer in their personal lives, may do the same at work. Ask how they juggle their schedule though, if there is a lot going on in their personal lives. Be careful with how you ask these questions though. Your HR department should be able to give you guidance in how to phrase things. Good luck. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Wednesday, January 25, 2012 12:19 PM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Interview Questions It would seem that questions like How do you feel about cannibalism? might also be out but might be far more helpful; than phone questions. On the serious side, when I was
Re: [Histonet] Interview Questions
Not upset in the least. Just posting my own questions and doubts within the parameters of the situation. When the Chinese philosopher who fell asleep under a tree and dreamt he was a butterfly and then spent the rest of his life asking if he was a human who fell asleep under a tree and dreamt he was a butterfly or was really a butterfly dreaming he was a human who fell asleep under a tree who? Wouldn't say he at all took offense to the situation; pondering, reflecting and just asking a question. Ray Seattle Sent from my Bedroom Wireless Laptop - Original Message - From: joelle weaver joellewea...@hotmail.com To: koelli...@comcast.net Sent: Wednesday, January 25, 2012 7:26:37 PM Subject: Re: [Histonet] Interview Questions Well I am sorry that you took such offense, but some jobs do have say words/minute typing for example. I guess the variation in qualified individuals leads me to not be upset to be asked to demonstrate tasks within the assigned duties. I think maybe you have simplifed a bit too. I think all those professions,such as attorneys have to do much more than you indicate_sorry this upset you Sent from my Verizon Wireless BlackBerry -Original Message- From: koelli...@comcast.net Date: Thu, 26 Jan 2012 02:59:49 To: joellewea...@hotmail.com Cc: trathbo...@somerset-healthcare.com; billodonn...@catholichealth.net; sbree...@nmda.nmsu.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Interview Questions This is certainly an interesting thread and I generally hate to get into these ever but I still can't figure out one thing and never have over all these years in pathology. What other endeavor in life and job seeking is an on-the-spot demo that you can do something required at a job interview? Does a lawyer have to go into a courtroom for 5 minutes and show he/she can say I object? Does a sanitation worker have to go round the block once and show he/she can empty 9 cans in 5 minutes? Does a doctor need to show he/she can use a stethoscope? Does a bricklayer have to show he/she can lay 20 bricks in 2 minutes? Or fail the interview? Does a med tech have to show they can stain 6 tubes with CD4 and CD 8 and successfully put them on a flow cytometer? Does an actuary have to show they can really add 100 4-digit numbers on a calculator without a mistake? Does a grocery bagger boy /girl have to show they can put x number of items in 3 bags? Does a Pathologist have to show they know how to turn on a microscope and look through it? Does a peanut counter have to show they can count peanuts? I just can't get into my mind the necessity of someone having to cut to show they can cut? What other profession does this at an interview? Now certainly you can come up with scenarios where it might be important to find out. A brand new histotech whose only cut 3 blocks in their life. A tech from the deepest, darkest nether regions of the earth where you cannot check on their background. But a tech whose has been working cutting the last 3 or 7 or 15 years and you've verified with a previous company that is exactly what they did; how will them cutting for 10 minutes further stratify them into yes or no categories. If 2 potential techs cut and one finishes in 9 minutes and one in 10 minutes, is that a true qualifier or disqualifier of what they can do cutting? There are a myriad of things I'd love to know and always ask; personality, job knowledge, wants, desires, needs, ambitions, etc, etc, etc. My blood pressure skyrockets when I give blood because I HATE anyone sticking a needle in me. But I have a really needed blood type. Should nervousness each time disqualify me. This still boggles my mind about what is being accomplished with cutting during an interview? Ray Seattle, WA From: joelle weaver joellewea...@hotmail.com To: trathbo...@somerset-healthcare.com, billodonn...@catholichealth.net, sbree...@nmda.nmsu.edu, Histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, January 25, 2012 10:02:39 AM Subject: RE: [Histonet] Interview Questions Love this! I always want to do demonstration during technical interviews, but usually get shot down from managers and argued with in general, as in people don't feel that they should have to prove they can do histology. This perception, I never got, because I always saw it as in a job interview-in what other situation are you more trying to prove or impress with your knowledge, attitude, skills and experience? If you do bench work, you can tell in just a few minutes of observation much more information than you could get with quite a few questions. To be fair, I take into account nervousness, being closely observed, and lack of familiarity with equipment etc. I don't know, I think its fair if those are important skills to the position/role. Was not sure if Sara's job was mostly technical though, so thought I might keep it general. Joelle
Re: [Histonet] Interview Questions
My most hated question in interviews is where do you see yourself in 5 years?answer - in your job asking stupid questions! On Thu, Jan 26, 2012 at 5:48 AM, koelli...@comcast.net wrote: Not upset in the least. Just posting my own questions and doubts within the parameters of the situation. When the Chinese philosopher who fell asleep under a tree and dreamt he was a butterfly and then spent the rest of his life asking if he was a human who fell asleep under a tree and dreamt he was a butterfly or was really a butterfly dreaming he was a human who fell asleep under a tree who? Wouldn't say he at all took offense to the situation; pondering, reflecting and just asking a question. Ray Seattle Sent from my Bedroom Wireless Laptop - Original Message - From: joelle weaver joellewea...@hotmail.com To: koelli...@comcast.net Sent: Wednesday, January 25, 2012 7:26:37 PM Subject: Re: [Histonet] Interview Questions Well I am sorry that you took such offense, but some jobs do have say words/minute typing for example. I guess the variation in qualified individuals leads me to not be upset to be asked to demonstrate tasks within the assigned duties. I think maybe you have simplifed a bit too. I think all those professions,such as attorneys have to do much more than you indicate_sorry this upset you Sent from my Verizon Wireless BlackBerry -Original Message- From: koelli...@comcast.net Date: Thu, 26 Jan 2012 02:59:49 To: joellewea...@hotmail.com Cc: trathbo...@somerset-healthcare.com; billodonn...@catholichealth.net; sbree...@nmda.nmsu.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Interview Questions This is certainly an interesting thread and I generally hate to get into these ever but I still can't figure out one thing and never have over all these years in pathology. What other endeavor in life and job seeking is an on-the-spot demo that you can do something required at a job interview? Does a lawyer have to go into a courtroom for 5 minutes and show he/she can say I object? Does a sanitation worker have to go round the block once and show he/she can empty 9 cans in 5 minutes? Does a doctor need to show he/she can use a stethoscope? Does a bricklayer have to show he/she can lay 20 bricks in 2 minutes? Or fail the interview? Does a med tech have to show they can stain 6 tubes with CD4 and CD 8 and successfully put them on a flow cytometer? Does an actuary have to show they can really add 100 4-digit numbers on a calculator without a mistake? Does a grocery bagger boy /girl have to show they can put x number of items in 3 bags? Does a Pathologist have to show they know how to turn on a microscope and look through it? Does a peanut counter have to show they can count peanuts? I just can't get into my mind the necessity of someone having to cut to show they can cut? What other profession does this at an interview? Now certainly you can come up with scenarios where it might be important to find out. A brand new histotech whose only cut 3 blocks in their life. A tech from the deepest, darkest nether regions of the earth where you cannot check on their background. But a tech whose has been working cutting the last 3 or 7 or 15 years and you've verified with a previous company that is exactly what they did; how will them cutting for 10 minutes further stratify them into yes or no categories. If 2 potential techs cut and one finishes in 9 minutes and one in 10 minutes, is that a true qualifier or disqualifier of what they can do cutting? There are a myriad of things I'd love to know and always ask; personality, job knowledge, wants, desires, needs, ambitions, etc, etc, etc. My blood pressure skyrockets when I give blood because I HATE anyone sticking a needle in me. But I have a really needed blood type. Should nervousness each time disqualify me. This still boggles my mind about what is being accomplished with cutting during an interview? Ray Seattle, WA From: joelle weaver joellewea...@hotmail.com To: trathbo...@somerset-healthcare.com, billodonn...@catholichealth.net, sbree...@nmda.nmsu.edu, Histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, January 25, 2012 10:02:39 AM Subject: RE: [Histonet] Interview Questions Love this! I always want to do demonstration during technical interviews, but usually get shot down from managers and argued with in general, as in people don't feel that they should have to prove they can do histology. This perception, I never got, because I always saw it as in a job interview-in what other situation are you more trying to prove or impress with your knowledge, attitude, skills and experience? If you do bench work, you can tell in just a few minutes of observation much more information than you could get with quite a few questions. To be fair, I take into account nervousness, being closely observed, and lack of familiarity with equipment
