Re: [Histonet] Regarding Bone-Implant Contact Percentage
Hi how are you hoping to quantitate? do you have a software programme? Our latest paper on titanium implants (in press) measure BIC expressed as a percentage of implant surface examined. On Mon, Feb 13, 2012 at 1:57 PM, ragamouni sravanthi ragamouni.sravan...@gmail.com wrote: Hai,... Can any one help me with how to calculate percentage of bone-implant contact with implants in bone sections. Please can you anyone give me detailed explanation and the required equation.Ineed it in urgency. With Regards R.Sravanthi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] H. pylori
Happy Valentines Day! Our lab is looking into ordering H. pylori now and I was wondering what clone, or whose antibody everyone is using. As always, thanks for your responses. I know I can always count on this group to offer opinions and suggestions :) Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: H. pylori
I use BioCare Monoclonal Antibody with great results. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, February 14, 2012 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. pylori Happy Valentines Day! Our lab is looking into ordering H. pylori now and I was wondering what clone, or whose antibody everyone is using. As always, thanks for your responses. I know I can always count on this group to offer opinions and suggestions :) Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] new billing
can someone explain the new billing for the antibodies on blocks? not sure what they mean. can you just bill one antibody per block even tho you may do three or four? thanks so much anita dudley providence hosp mobile alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] new billing
You can charge for each antibody on each specimen. You cannot charge for the same antibody on multiple blocks of the same specimen. e.g. Sentinel node - Melan A, S100, HMB45 A1 - A4 = 3 charges not 12. Hope this makes sense.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Tuesday, February 14, 2012 10:30 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] new billing can someone explain the new billing for the antibodies on blocks? not sure what they mean. can you just bill one antibody per block even tho you may do three or four? thanks so much anita dudley providence hosp mobile alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] new billing
Now I am confused. Did you mean to state same antibody on multiple slides of the same specimen rather than multiple blocks of the same specimen? I am asking because of questions from breast cancer patients who have difficulty getting coverage from their insurance companies when multiple lymph nodes are submitted from a single surgery. Thanks, Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, February 14, 2012 8:36 AM To: anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new billing You can charge for each antibody on each specimen. You cannot charge for the same antibody on multiple blocks of the same specimen. e.g. Sentinel node - Melan A, S100, HMB45 A1 - A4 = 3 charges not 12. Hope this makes sense.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Tuesday, February 14, 2012 10:30 To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Subject: [Histonet] new billing can someone explain the new billing for the antibodies on blocks? not sure what they mean. can you just bill one antibody per block even tho you may do three or four? thanks so much anita dudley providence hosp mobile alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: H. pylori
Dako concentrate on the Ventana XT. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, February 14, 2012 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. pylori Happy Valentines Day! Our lab is looking into ordering H. pylori now and I was wondering what clone, or whose antibody everyone is using. As always, thanks for your responses. I know I can always count on this group to offer opinions and suggestions :) Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] new billing
Same antibody on multiple blocks of same specimen was what I was referring to. For the past couple of years, we could charge per block. Now it is per specimen. However, it is still per block for special stains. Just to be sure that is clear... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax From: Goins, Tresa [mailto:tgo...@mt.gov] Sent: Tuesday, February 14, 2012 10:48 To: Weems, Joyce; anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new billing Now I am confused. Did you mean to state same antibody on multiple slides of the same specimen rather than multiple blocks of the same specimen? I am asking because of questions from breast cancer patients who have difficulty getting coverage from their insurance companies when multiple lymph nodes are submitted from a single surgery. Thanks, Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, February 14, 2012 8:36 AM To: anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new billing You can charge for each antibody on each specimen. You cannot charge for the same antibody on multiple blocks of the same specimen. e.g. Sentinel node - Melan A, S100, HMB45 A1 - A4 = 3 charges not 12. Hope this makes sense.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Tuesday, February 14, 2012 10:30 To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Subject: [Histonet] new billing can someone explain the new billing for the antibodies on blocks? not sure what they mean. can you just bill one antibody per block even tho you may do three or four? thanks so much anita dudley providence hosp mobile alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Histology Job Opportunities
Hi Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Histology Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Histology Job Opportunities (HT or HTL): * CA - Southern - Histotech * FL - Treasure Coast - Histotech * IN - Histotech * Maine - Histotech * NY - Westchester - Histotech (experienced) * NC - Histotech 3rd shift (experienced) * NY - Western - Histotech * NY - NYC - Histotech * NJ - Histotech * NYC - Pathology Manager (commercial background) * NYC - Histology Supervisor * NY - Long Island - Cytotech * NJ - Cytotech * NY - Westchester - Cyto Prep Technician * OH - Histotech/ Histology Supervisor * PA - Histotech If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, Alisha Dynan, Founder K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 P: (617) 692-2949 F: (617) 507-8009 ali...@ka-recruiting.com www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] good source for APES to make silane treated slides?
Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 pat...@gwumc.edu BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:pat...@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] new billing Codes
Hi Histonetters, I have a great idea. You can come to Georgia for our meeting, our own Joyce Weems will be presenting a workshop on CPT coding at our meeting. The Georgia Society for Histotechnology - Region III meeting will be held at Callaway Gardens, Pine Mountain GA April 13-15th,. The Deadline for receiving the discounted hotel rate is March 13th, so act now. Remember The Mountain Creek Inn fills up fast during the spring which is golfing season here in Georgia. Making reservations with a credit card will hold your room, they will not apply the rate until you actually check in. Call now 1-800-225-5292 and use the GSH group # 78K711 to get the discounted room rate of $109. There are other rooming option rates in the program which can be found at http://www.histosearch.com/gsh/symposium.html. Also register early for the fantastic workshops GSH has lined up, like Joyce's and earn your CEUs. GSH has planned a great awards luncheon on Saturday as well as a dinner on Saturday night, details are in the program. Payment is easy with PayPal. You do not have to have an account with them to use this service and it is secure. Make payment by going to www.PayPal.com and send payment to the email address on the registration form. Plan to attend, bring the family for a vacation, Callaway Gardens is a wonderful family experience. If you have any questions please contact GSH President - Mike Ayers at lmay...@charter.net, GSH Vice President and Exhibit Liaison - Wanda Simons at wandr...@att.net or myself powell...@mercer.edu. Please pass this invitation to any who may not get this email. Come to Georgia and experience Histotechnology - Southern Style -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, February 14, 2012 10:55 AM To: Goins, Tresa; anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new billing Same antibody on multiple blocks of same specimen was what I was referring to. For the past couple of years, we could charge per block. Now it is per specimen. However, it is still per block for special stains. Just to be sure that is clear... Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax From: Goins, Tresa [mailto:tgo...@mt.gov] Sent: Tuesday, February 14, 2012 10:48 To: Weems, Joyce; anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new billing Now I am confused. Did you mean to state same antibody on multiple slides of the same specimen rather than multiple blocks of the same specimen? I am asking because of questions from breast cancer patients who have difficulty getting coverage from their insurance companies when multiple lymph nodes are submitted from a single surgery. Thanks, Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Tuesday, February 14, 2012 8:36 AM To: anita dudley; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] new billing You can charge for each antibody on each specimen. You cannot charge for the same antibody on multiple blocks of the same specimen. e.g. Sentinel node - Melan A, S100, HMB45 A1 - A4 = 3 charges not 12. Hope this makes sense.. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Tuesday, February 14, 2012 10:30 To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Subject: [Histonet] new billing can someone explain the new billing for the antibodies on blocks? not sure what they mean. can you just bill one antibody per block even tho you may do three or four? thanks so much anita dudley providence hosp mobile alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hight throughput IHC
Is anyone doing any type of high throughput IHC testing? If so I would love to pick your brain. Andrea T. Hooper, PhD andreahoo...@rocketmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] good source for APES to make silane treated slides?
Sigma Chemical, catalog number A3648 Geoff On 2/14/2012 11:18 AM, Joseph Madary wrote: Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 pat...@gwumc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] wrinkles
Dear histonet members First I wish every one a happy valentine day!!! At our hospital the lab temperature is not controlled. In summer it is very hot and in winter it is cold. When I trim the paraffin embedded tissue my sections are faultless . Now because it is winter I have a problem having wrinkles in the sectionsafter collecting them from the water bath. Does anyone recommend me to buy slide drying hotplate for the slides?especially for the IHC slides, if you recommend me to buy it at what temperature should I use it and how it would effect the baking time afterward? Thanks Wassan/Histotechnican Shorsh General Hospital North of Iraq ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hey histo gurus: Just a little twist for you. We are planning on doing GMS and PASD on frozen skin sections. Is there any change in staining protocol for paraffin vs frozen. We plan on fixing in ETOH. I have a co-worker saying that we don't need to use diastase on the PASD because there are already active enzymes in the tissue as it is fresh. He also said that we don't need to heat the silver for the GMS. Not sure why. These changes don't seem right to me. But I have been wrong before (Yes, I admit it). Thanks for all the wisdom out there floating in the electrons. We are staining for fungus BTW. Claire ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] H. pylori
Keep in mind that many of the commercially-available monoclonal antibodies to H. pylori do not label Helicobacter helilmannii, often seen in children, especially those with exposure to animals. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Rathborne, Toni trathbo...@somerset-healthcare.com 2/14/2012 10:21 AM Happy Valentines Day! Our lab is looking into ordering H. pylori now and I was wondering what clone, or whose antibody everyone is using. As always, thanks for your responses. I know I can always count on this group to offer opinions and suggestions :) Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC and bone implants
Del, I have a few questions for you to better understand your problem. 1) Are you talking about decalcified paraffin sections or undecalcified resin embedded specimens? 2) Is the implant washing off or both the bone and implant? 3) What type of implant/material is it? 4) If decalcified and paraffin embedded, what temperature and how long do you melt your specimens to the slide on the slide warmer? 5) If undecalcified and resin embedded, what type of resin and how thick are your sections? 6) Lastly, what step do you notice that the section or implant becomes detached from the slide? Please forgive me for the list of questions, but a little more information will help to pinpoint the problem and help get straight to the best corrective action. Best Regards, Jack Jack Ratliff Chairman, Hard Tissue Committee - National Society for Histotechnology Senior Histologist, Biomimetic Therapeutics, Inc. From: dphill...@vetmed.lsu.edu To: Histonet@lists.utsouthwestern.edu Date: Mon, 13 Feb 2012 09:53:15 -0600 CC: Subject: [Histonet] IHC and bone implants Does anyone have any suggestions for staining bone implants? I am having trouble with wash offs. I use charged slides and they are still washing off. Thanks Del ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
About not using diastase for PASD because there are active enzymes in the tissue: that is wrong because those are the same enzymes active in any tissue just before it is fixed. Also remember that you are going to fix with EthOL. The whole issue behind the PASD (as you well know) is to compare the results of the PAS with and without diastase, so your co-worker is wrong. As to not heating the silver for GMS I do see any reason to not doing that. If you do not heat it you will get something different to GMS. Perhaps your co-worker is fearful that the section may peel-off because of the heat but, other than that, there is no reason. René J. --- On Tue, 2/14/12, Ingles Claire cing...@uwhealth.org wrote: From: Ingles Claire cing...@uwhealth.org Subject: [Histonet] (no subject) To: histonet@lists.utsouthwestern.edu Date: Tuesday, February 14, 2012, 2:22 PM Hey histo gurus: Just a little twist for you. We are planning on doing GMS and PASD on frozen skin sections. Is there any change in staining protocol for paraffin vs frozen. We plan on fixing in ETOH. I have a co-worker saying that we don't need to use diastase on the PASD because there are already active enzymes in the tissue as it is fresh. He also said that we don't need to heat the silver for the GMS. Not sure why. These changes don't seem right to me. But I have been wrong before (Yes, I admit it). Thanks for all the wisdom out there floating in the electrons. We are staining for fungus BTW. Claire ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] wrinkles
If you consider that it\\your lab is cold now in winter, have you checked out the temperature in the water bath? Perhaps it is too cold to allow the sections to expand. If the water bath keeps its temperature (about 45ºC) the room temperature is irrelevant. René J. --- On Tue, 2/14/12, wassan alkadhumi w_alkadh...@yahoo.com wrote: From: wassan alkadhumi w_alkadh...@yahoo.com Subject: [Histonet] wrinkles To: h n histonet@lists.utsouthwestern.edu Date: Tuesday, February 14, 2012, 1:44 PM Dear histonet members First I wish every one a happy valentine day!!! At our hospital the lab temperature is not controlled. In summer it is very hot and in winter it is cold. When I trim the paraffin embedded tissue my sections are faultless . Now because it is winter I have a problem having wrinkles in the sectionsafter collecting them from the water bath. Does anyone recommend me to buy slide drying hotplate for the slides?especially for the IHC slides, if you recommend me to buy it at what temperature should I use it and how it would effect the baking time afterward? Thanks Wassan/Histotechnican Shorsh General Hospital North of Iraq ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] QA by pathologists
Akemi Allison asks: What % QA do most pathologists do internally? I'm trying to update my AP Manual. My last CLIA inspection, they told me to put in my AP Manual that it is done and get a binder labeled discrepancies. If there are any... As far as I know, there is no percentage requirement like the burdensome and useless 10% rescreen on Pap smears. In my travels I've seen: One three-pathologist group did 100% review before sign-out. At least two services I've seen did a 10% review, cases chosen at random. This seemed really pointless. One service did a 10% review, with the originating pathologist choosing the cases to be reviewed. This fairly productive. One five-pathologist service meticulously documented informal consultation, both in the report and on a separate piece of paper. They aimed at, and got, a 2.8% review. In a multiple pathologist practice, I think this was the most productive method I ever saw. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New DNA Probes for Kappa and Lambda
In the past we have used a cocktail DNA probe (ISH) for Kappa and Lambda. Has anybody had any experience validating and setting up the new probes from Ventana which we have to mix the four probes together and form our own cocktail? Please share with me your thoughts. I am seriously considering sending this test out because of the infrequency, time, ASR hassle, costs of separate detection kits, etc. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
The reason for using diastase is to remove the glycogen from the tissues. AND if you do not heat the methenamine silver solution for the GMS it will take forever to stain, in fact all you will get is background spots. This I can verify, have you ever forgot to turn on the water bath before staining? - several hours later - nuthin!! Who ever is giving you the advice I would wonder about their histotechnology education and training (in fact have they had any training at all?). Do not listen to them. Extrapolate from cytology. GMS and DiPAS have been done on cytology smears fixed in ethanol as well as air-dried for years see: Loughman NT. Pneumocystis carinii: rapid diagnosis with the microwave oven Acta Cytologica. 33(3):416-7, 1989 May-Jun. PINTOZZI, R. L. Modified Grocott's methenamine silver nitrate method for quick staining of Pneumocystis carinii J Clin Pathol 1978 31: 803-805 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, 15 February 2012 6:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hey histo gurus: Just a little twist for you. We are planning on doing GMS and PASD on frozen skin sections. Is there any change in staining protocol for paraffin vs frozen. We plan on fixing in ETOH. I have a co-worker saying that we don't need to use diastase on the PASD because there are already active enzymes in the tissue as it is fresh. He also said that we don't need to heat the silver for the GMS. Not sure why. These changes don't seem right to me. But I have been wrong before (Yes, I admit it). Thanks for all the wisdom out there floating in the electrons. We are staining for fungus BTW. Claire ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] endogeneous peroxidase blocking
Morning All Posing this question for a colleague. Suggestions for immunohistochemistry blocking for endogeneous peroxidase probably caused by red blood cells in mouse lung tissue. Using 1% hydrogen peroxide/dist.water for 15mins and still getting non specific staining. Thank you Marilyn Medical School UCT Dept of Surgery J50/26 or 30 Surgical research. OMB Groote Schuur Hospital Observatory 021-4066227 ### UNIVERSITY OF CAPE TOWN This e-mail is subject to the UCT ICT policies and e-mail disclaimer published on our website at http://www.uct.ac.za/about/policies/emaildisclaimer/ or obtainable from +27 21 650 9111. This e-mail is intended only for the person(s) to whom it is addressed. If the e-mail has reached you in error, please notify the author. If you are not the intended recipient of the e-mail you may not use, disclose, copy, redirect or print the content. If this e-mail is not related to the business of UCT it is sent by the sender in the sender's individual capacity. ### ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
