RE: [Histonet] Block Storage
This is a very sticky issue, when I setup inhouse labs I always present the argument that during inspections the lab that produces a report should have access to all test material which includes blocks and slides per the CAP checklist. Ann trying approaching it from that angle. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Angelo Sent: Tuesday, March 20, 2012 6:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they perform Technical component on if they do not perform the professional componentor should they have the facility performing the professional component store them? Who is ultimately responsible? Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: MSDS regarding waste alcohol, xylene, etc
We do keep minimum volumes of alcohol and xylene in the safety cabinet. But we can't order so much that some are stored on the floor outside the cabinet--keep just enough that you use in a reasonable time frame. We get alcohol in hospital, weekly, so it is not a problem. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -Original Message- From: Tony Henwood (SCHN) [mailto:tony.henw...@health.nsw.gov.au] Sent: Tuesday, March 20, 2012 6:36 PM To: Sherwood, Margaret; 'Troyer, Dean A.'; histonet@lists.utsouthwestern.edu Subject: RE: MSDS regarding waste alcohol, xylene, etc Good, though I can see a conflict of interest between the water authorities and fire safety authorities. One say keep and dispose of appropriately. The other says if you keep then store it in minimum volumes and in appropriate safety cabinets. Now to keep both happy (as well as yourself) - that could be difficult Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: Sherwood, Margaret [mailto:msherw...@partners.org] Sent: Wednesday, 21 March 2012 12:14 AM To: Tony Henwood (SCHN); 'Troyer, Dean A.'; histonet@lists.utsouthwestern.edu Subject: RE: MSDS regarding waste alcohol, xylene, etc I thought I would add my 2 cents to this discussion. Our hazardous waste is regulated by the State and the MWRA (Mass Water Resource Association). Basically, we cannot throw anything down the drains. Therefore, we collect our alcohols, etc., label with hazardous waste labels, and store in special satellite accumulaton areas (usually hoods). We have an outside waste company that collects our waste weekly. We cannot leave hazardous waste containers (i.e. alcohol or xylene, etc.)or supplies of such outside a safety cabinet. Therefore, we are not allowed to stockpile solutions. Not sure what the regs are in your area. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Monday, March 19, 2012 6:01 PM To: 'Troyer, Dean A.'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: MSDS regarding waste alcohol, xylene, etc I hope that these are stored in flammable cupboards/rooms and that maximum allowable volumes are adhered to (that will definitely upset them). As to waste liquids must be stored within sight of where they are generated- I hope that all safety storage requirements are adhered to since they must take precedence. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Troyer, Dean A. Sent: Tuesday, 20 March 2012 1:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MSDS regarding waste alcohol, xylene, etc We were considering ways to tidy up a hospital pathology grossing room where specimens are accessioned, grossed, and frozen sections performed. There is one tissue processor in the room. The room has only one doorway and has no windows. Several large plastic jugs of liquid waste (alcohols, xylene) are retained within the room for weekly pick up by a waste disposal service. It was suggested that the waste jugs take up valuable floor space and might be stored in an adjacent room, equally well ventilated, where additional tissue processors are located. One of our laboratory supervisors indicated that the waste jugs could not be stored outside the room because waste liquids must be stored within site of where they are generated. I believe the MSDS sheets/regulations were cited, but not sure. Is there experience with regulations that address this? It seems counterintuitive to store flammable liquids in a room such as this with a number of people there all day and evenings. Particularly if there is an option of storing them in an adjacent room with releatively fewer personnel. Dean Troyer ___ Histonet mailing list
[Histonet] Thermo Scientific Aquamount procedure?
I have a bottle of Thermo Scientific Aquamount and a path who is insisting that I start doing AEC chromogen on our immuno slides. We're still hand staining immunos, although we have a Benchmark Ultra now, the doctors are dragging their feet on reading the validation slides. Anyway, how exactly do I use the Aquamount? I seem to have lost the info sheet that came with it and can't find anything online. I used some aqueous mounting media ages ago and that involved just putting drops right on the slide and letting it harden. Or do I use it just like regular mountant, with a coverslip? Thanks so much for any help - Caitlin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Neurofilament - LFB combo -- problems
To anyone who performs a NF-LFB (+PAS) combo stain using a Ventana Benchmark: I have been trying to stain a nerve (cross and longitudinal section) and it keeps falling off of the slide after the neurofilament stain. It's been cut onto a charged slide and has been baked 20 minutes prior to IHC staining. After IHC staining, we have rinsed in Dawn-water per protocol; by then the longitudinal section is peeling off the slide. I tried skipping the Dawn rinse and have gone to straight 95% alcohol and 1 of 4 sections survived on the slide (others fell off). Tissue problem - the nerve is well myelinated and fatty. If I drop the water bath temp too low, the nerve doesn't flatten well; if the temp is too high, it's shredding and spreading. Has anyone out there had this problem and resolved it? Or do you have a protocol that might have avoided it completely? Any suggestions would be appreciated. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for Used cassette Printer
I am looking for a used VCP cassette printer from Leica/Surgipath. My print head finally went out. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Thermo Scientific Aquamount procedure?
Use it as a regular mountant and coverslip. René J. --- On Wed, 3/21/12, Caitlin Hesketh caithesk...@gmail.com wrote: From: Caitlin Hesketh caithesk...@gmail.com Subject: [Histonet] Thermo Scientific Aquamount procedure? To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 21, 2012, 9:08 AM I have a bottle of Thermo Scientific Aquamount and a path who is insisting that I start doing AEC chromogen on our immuno slides. We're still hand staining immunos, although we have a Benchmark Ultra now, the doctors are dragging their feet on reading the validation slides. Anyway, how exactly do I use the Aquamount? I seem to have lost the info sheet that came with it and can't find anything online. I used some aqueous mounting media ages ago and that involved just putting drops right on the slide and letting it harden. Or do I use it just like regular mountant, with a coverslip? Thanks so much for any help - Caitlin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Block Storage
Depending on the state regulations and the laboratory policies,blocks are stored during different periods of time before being discarded. I used to store them for 9 years. René J. --- On Wed, 3/21/12, Nails, Felton flna...@texaschildrens.org wrote: From: Nails, Felton flna...@texaschildrens.org Subject: RE: [Histonet] Block Storage To: 'Ann Angelo' thisis...@aol.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, March 21, 2012, 9:00 AM This is a very sticky issue, when I setup inhouse labs I always present the argument that during inspections the lab that produces a report should have access to all test material which includes blocks and slides per the CAP checklist. Ann trying approaching it from that angle. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Angelo Sent: Tuesday, March 20, 2012 6:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they perform Technical component on if they do not perform the professional componentor should they have the facility performing the professional component store them? Who is ultimately responsible? Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Water Quality
In regards to CAP's Lab General Checklist questions regardin= g water quality: Is deionized water consid= ered CLRW (Clinical Laboratory Reagent Water)? Is water testin= g necessary for a Histology Lab? If so, how do small labs test f= or resisitivity and perform microbiology cultures? Would anyone be willing to share their procedure for Water Quality for a small, private pathology lab? Thank= s, Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Water Quality
When you introduce the words reagent quality it means that a chemical manufacturer has done specific tests to assure that the water complies with such designation. Deionized water is usable for solutions preparations but the issue resides in the deionizer that has to be tested as to its performance. No small laboratory has the conditions required to perform microbiology cultures, nor it is really necessary to do under normal working histology conditions. If these are new CAP requirements it seems to me that CAP is going beyond reasonable requirements. René J. --- On Wed, 3/21/12, lau...@blufrogpath.com lau...@blufrogpath.com wrote: From: lau...@blufrogpath.com lau...@blufrogpath.com Subject: [Histonet] Water Quality To: Histonet post histonet@lists.utsouthwestern.edu Date: Wednesday, March 21, 2012, 10:27 AM In regards to CAP's Lab General Checklist questions regardin= g water quality: Is deionized water consid= ered CLRW (Clinical Laboratory Reagent Water)? Is water testin= g necessary for a Histology Lab? If so, how do small labs test f= or resisitivity and perform microbiology cultures? Would anyone be willing to share their procedure for Water Quality for a small, private pathology lab? Thank= s, Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Water Quality
Our DI tanks are provided and serviced by Seimens. They do the testing. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 21, 2012 9:35 AM To: Histonet post; lau...@blufrogpath.com Subject: Re: [Histonet] Water Quality When you introduce the words reagent quality it means that a chemical manufacturer has done specific tests to assure that the water complies with such designation. Deionized water is usable for solutions preparations but the issue resides in the deionizer that has to be tested as to its performance. No small laboratory has the conditions required to perform microbiology cultures, nor it is really necessary to do under normal working histology conditions. If these are new CAP requirements it seems to me that CAP is going beyond reasonable requirements. René J. --- On Wed, 3/21/12, lau...@blufrogpath.com lau...@blufrogpath.com wrote: From: lau...@blufrogpath.com lau...@blufrogpath.com Subject: [Histonet] Water Quality To: Histonet post histonet@lists.utsouthwestern.edu Date: Wednesday, March 21, 2012, 10:27 AM In regards to CAP's Lab General Checklist questions regardin= g water quality: Is deionized water consid= ered CLRW (Clinical Laboratory Reagent Water)? Is water testin= g necessary for a Histology Lab? If so, how do small labs test f= or resisitivity and perform microbiology cultures? Would anyone be willing to share their procedure for Water Quality for a small, private pathology lab? Thank= s, Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotechnologist (Lead) on the 3rd shift needed in Ft. Myers, FL
Allied Search Partners is currently looking for a qualified applicant for a Histotechnologist for a 3rd shift Lead position available in a Fort Myers, FL laboratory. Position: Histotechnologist Schedule: 3rd shift/full time permanent position Summary: Perform a variety of routine and specialized histology techniques and procedures. Embedding, Microtomy, Grossing, Processing, and HE staining Special Staining Equipment maintenance Requirements: FL Laboratory License Previous experience with automated IHC Technical and QC protocols AA or BS/BA degree in life science Benefits: Competitive salaries, Health, Dental, Life Disability insurances, a section 125 plan, a 401K, FSA, ESPP and relocation assistance. To Apply: Please send resume to bran...@alliedsearchpartners.com mailto:bran...@alliedsearchpartners.com -- *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with remove. Brannon Owens, Recruitment Manager LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.linkedin.com/pub/brannon-owens/28/528/823 Allied Search Partners T: 888.388.7571 ext. 106 F: 888.388.7572 www.alliedsearchpartners.com http://www.alliedsearchpartners.com/ Tell us about your experience with ASP by clicking on this link: http://ratepoint.com/tellus/82388 http://www.alliedsearchpartners.com/ This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] April fool's prank
Any ideal for April fool's prank? Tunde Ajibade BS, HTL(ASCP)QIHC CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Block Storage
Rene I think this question is eluding to a Physician owned lab and who is responsible for storing the blocks not how long they are retained? From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Wednesday, March 21, 2012 9:20 AM To: 'Ann Angelo'; histonet@lists.utsouthwestern.edu; Nails, Felton Subject: RE: [Histonet] Block Storage Depending on the state regulations and the laboratory policies,blocks are stored during different periods of time before being discarded. I used to store them for 9 years. René J. --- On Wed, 3/21/12, Nails, Felton flna...@texaschildrens.org wrote: From: Nails, Felton flna...@texaschildrens.org Subject: RE: [Histonet] Block Storage To: 'Ann Angelo' thisis...@aol.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, March 21, 2012, 9:00 AM This is a very sticky issue, when I setup inhouse labs I always present the argument that during inspections the lab that produces a report should have access to all test material which includes blocks and slides per the CAP checklist. Ann trying approaching it from that angle. -Original Message- From: histonet-boun...@lists.utsouthwestern.eduhttp://us.mc1621.mail.yahoo.com/mc/compose?to=histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.eduhttp://us.mc1621.mail.yahoo.com/mc/compose?to=histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Angelo Sent: Tuesday, March 20, 2012 6:58 PM To: histonet@lists.utsouthwestern.eduhttp://us.mc1621.mail.yahoo.com/mc/compose?to=histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they perform Technical component on if they do not perform the professional componentor should they have the facility performing the professional component store them? Who is ultimately responsible? Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.eduhttp://us.mc1621.mail.yahoo.com/mc/compose?to=Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.eduhttp://us.mc1621.mail.yahoo.com/mc/compose?to=Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Neurofilament - LFB combo -- problems
Karen writes: To anyone who performs a NF-LFB (+PAS) combo stain using a Ventana Benchmark: I have been trying to stain a nerve (cross and longitudinal section) and it keeps falling off of the slide after the neurofilament stain. It's been cut onto a charged slide and has been baked 20 minutes prior to IHC staining. After IHC staining, we have rinsed in Dawn-water per protocol; by then the longitudinal section is peeling off the slide. I tried skipping the Dawn rinse and have gone to straight 95% alcohol and 1 of 4 sections survived on the slide (others fell off). Tissue problem - the nerve is well myelinated and fatty. If I drop the water bath temp too low, the nerve doesn't flatten well; if the temp is too high, it's shredding and spreading. Has anyone out there had this problem and resolved it? Or do you have a protocol that might have avoided it completely? Any suggestions would be appreciated. Hi Karen, I don't know if any of this will help you, but here are a few suggestions. You can treat this tissue like we would brain or bone, after the water is drained off the section, lay the slides flat, tissue side up, on a slide warmer at lower temp, maybe 37-45 degrees C. You can try overnight and see if this helps. You can also try using Haupt's adhesive treated slides, they use this for plastic sections and bone and it tends to work pretty well. You can buy it commercially from Dorn and Hart, or you can make your own. The recipe is on the internet. Maybe use a combination of both? Try baking it a little longer too, 1-2 hours at 60 degrees if the above doesn't work. I know how frustrating this can be! Good luck. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Block Storage-another question
If the pod lab is in NJ and the reading lab is in NY, which guide lines do you follow. NYS requires us to save our blocks for 20 years. Due to storage issues, I would rather the pod lab store the blocks. Cindy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 21, 2012 10:20 AM To: 'Ann Angelo'; histonet@lists.utsouthwestern.edu; FeltonNails Subject: RE: [Histonet] Block Storage Depending on the state regulations and the laboratory policies,blocks are stored during different periods of time before being discarded. I used to store them for 9 years. René J. --- On Wed, 3/21/12, Nails, Felton flna...@texaschildrens.org wrote: From: Nails, Felton flna...@texaschildrens.org Subject: RE: [Histonet] Block Storage To: 'Ann Angelo' thisis...@aol.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, March 21, 2012, 9:00 AM This is a very sticky issue, when I setup inhouse labs I always present the argument that during inspections the lab that produces a report should have access to all test material which includes blocks and slides per the CAP checklist. Ann trying approaching it from that angle. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Angelo Sent: Tuesday, March 20, 2012 6:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they perform Technical component on if they do not perform the professional componentor should they have the facility performing the professional component store them? Who is ultimately responsible? Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] April fool's prank
I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's eyepieces. Vaseline on the coffee cup handles (don't all pathologists consume Mass Quantities of the stuff???). A tray of completely blank slides, all properly numbered for the day's work? Feed me some good ones, too. I'm down to my last week and I need to be remembered when I'm gone so they won't call me to do p.r.n.! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 100, Issue 27
I would be very careful when you say you need a CLIA certified tech to do gross (high complexity testing). You actually need someone who meets the CLIA requirements to perform High Complexity Testing. You can have a tech who is certified, but they may not meet the educational requirements. That person MUST have 60 semester hours, 24 of those in science, 6 hours must be Biology and 6 must be Chemistry and documented training in the area of high complexity testing. It has NOTHING to with certification, unfortunately. Kelly D. Boyd, BS, HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] commercial antibody diluent with carrier proteins SUGGESTIONS?
Hi all, I would like to get a commercially prepared antibody diluent with carrier proteins. I have been told Dako has a decent one but I am trying to find a different vendor. I see a few places with diluent but none that have background reducing substances/carrier protein type thing. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 pat...@gwumc.edu BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:pat...@gwumc.edu N:Madary;Joseph ORG:;Pathology TITLE:Senior Research Assistant TEL;PREF;FAX:202 994-5056 END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] April fool's prank
What a horrifying idea to put charcoal -or any material that produces dust- on a microscope. I like the blank slides idea though. Van: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] namens Breeden, Sara [sbree...@nmda.nmsu.edu] Verzonden: woensdag 21 maart 2012 16:27 Aan: Tunde Ajibade; histonet-boun...@lists.utsouthwestern.edu CC: histonet@lists.utsouthwestern.edu Onderwerp: RE: [Histonet] April fool's prank I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's eyepieces. Vaseline on the coffee cup handles (don't all pathologists consume Mass Quantities of the stuff???). A tray of completely blank slides, all properly numbered for the day's work? Feed me some good ones, too. I'm down to my last week and I need to be remembered when I'm gone so they won't call me to do p.r.n.! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] April fool's prank
While I'm new at histology, I am a master of pranks. Here's a list of some fun ones I've done to co-workers over that years...that haven't gotten me fired or killed yet ;) 1. fill their desk space with packing peanuts 2. If you have the time, post-it note their entire office, office wall, ceiling and desk with the multi colored post-it notes...it's pretty and funny 3. Find a dead keyboard in the IT department. Take it home, fill the areas around the keys with dirt and chia-pet seeds. Wait for them to sprout. Come in early in the morning and replace someones keyboard with this one (don't actually plug it into the computer) 4. Pop out all the keys on the keyboard that spell out the persons user name...hide them around their desk. 5. Wait for them to walk away, change their monitor settings, then change the language to something they don't know so they don't know how to get it back to normal. 6. If they hate the smell of bananas, your lotion, etc., put a peel, open bottle, whatever hidden behind their desk. 7. Tilt their desk up just slightly in the back so all their pens roll off, but it's not obvious that the desk has been lifted. Enjoy! Kara Lee Date: Wed, 21 Mar 2012 09:27:11 -0600 From: sbree...@nmda.nmsu.edu To: tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank CC: histonet@lists.utsouthwestern.edu I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's eyepieces. Vaseline on the coffee cup handles (don't all pathologists consume Mass Quantities of the stuff???). A tray of completely blank slides, all properly numbered for the day's work? Feed me some good ones, too. I'm down to my last week and I need to be remembered when I'm gone so they won't call me to do p.r.n.! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Block Storage-another question
I would think that if NYS is involved in anyway you have to hold those blocks for 20 years. When they inspect you do they inspect both labs? Do both labs hold a NYS permit? For cetain I would call NYS before you discard anything. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse [cp...@x-celllab.com] Sent: Wednesday, March 21, 2012 11:25 AM To: 'Rene J Buesa'; 'Ann Angelo'; histonet@lists.utsouthwestern.edu; 'FeltonNails' Subject: RE: [Histonet] Block Storage-another question If the pod lab is in NJ and the reading lab is in NY, which guide lines do you follow. NYS requires us to save our blocks for 20 years. Due to storage issues, I would rather the pod lab store the blocks. Cindy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 21, 2012 10:20 AM To: 'Ann Angelo'; histonet@lists.utsouthwestern.edu; FeltonNails Subject: RE: [Histonet] Block Storage Depending on the state regulations and the laboratory policies,blocks are stored during different periods of time before being discarded. I used to store them for 9 years. René J. --- On Wed, 3/21/12, Nails, Felton flna...@texaschildrens.org wrote: From: Nails, Felton flna...@texaschildrens.org Subject: RE: [Histonet] Block Storage To: 'Ann Angelo' thisis...@aol.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, March 21, 2012, 9:00 AM This is a very sticky issue, when I setup inhouse labs I always present the argument that during inspections the lab that produces a report should have access to all test material which includes blocks and slides per the CAP checklist. Ann trying approaching it from that angle. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Angelo Sent: Tuesday, March 20, 2012 6:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they perform Technical component on if they do not perform the professional componentor should they have the facility performing the professional component store them? Who is ultimately responsible? Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] April fool's prank
Please tell me you are kidding about all this stuff! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Wednesday, March 21, 2012 10:43 AM To: sbree...@nmda.nmsu.edu; tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank While I'm new at histology, I am a master of pranks. Here's a list of some fun ones I've done to co-workers over that years...that haven't gotten me fired or killed yet ;) 1. fill their desk space with packing peanuts 2. If you have the time, post-it note their entire office, office wall, ceiling and desk with the multi colored post-it notes...it's pretty and funny 3. Find a dead keyboard in the IT department. Take it home, fill the areas around the keys with dirt and chia-pet seeds. Wait for them to sprout. Come in early in the morning and replace someones keyboard with this one (don't actually plug it into the computer) 4. Pop out all the keys on the keyboard that spell out the persons user name...hide them around their desk. 5. Wait for them to walk away, change their monitor settings, then change the language to something they don't know so they don't know how to get it back to normal. 6. If they hate the smell of bananas, your lotion, etc., put a peel, open bottle, whatever hidden behind their desk. 7. Tilt their desk up just slightly in the back so all their pens roll off, but it's not obvious that the desk has been lifted. Enjoy! Kara Lee Date: Wed, 21 Mar 2012 09:27:11 -0600 From: sbree...@nmda.nmsu.edu To: tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank CC: histonet@lists.utsouthwestern.edu I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's eyepieces. Vaseline on the coffee cup handles (don't all pathologists consume Mass Quantities of the stuff???). A tray of completely blank slides, all properly numbered for the day's work? Feed me some good ones, too. I'm down to my last week and I need to be remembered when I'm gone so they won't call me to do p.r.n.! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] April fool's prank
There are a few I remember from the past: Telling the pathologists that the processor has been delayed or stopped running in the alcohols. If you ever want to see them go bonkers - that one will do it. Loosening or taking out of bulbs from microscopes - not a very nice thing to do but I've heard of it. Also make sure that the back up bulbs have been safely secured elsewhere. No I've not done this..but I'm told a pathologist or tech without their microscope can be pretty intense. There is always the sick call thing - but it has grown sort of old. Having a pathologist read a fake slide. We used an oak leaf - made out all the paperwork and all of it tied into 'woody' things. Had an unsuspecting green tech gross it in and so on. It's amazing what you can pull off on newbies. Put Vaseline on the microscope stage - slides will go 'slip sliddn' away as they try to adjust x/y positions. Tape or block (pennies sometimes work) their top center desk drawer shut - they can't open other drawers if that one won't open. (or am I dating myself). Replace sharp pencils and pens with dull pencils(stubs if possible) and pens that don't work or are difficult. There is always replacing their red pen ink with blue or black ink. A favorite is replacing a keyboard or loosening a connection on their computer or dictation machine. If it's a 'dead' keyboard they don't know what to do initially - if it's a wrong keyboard it's even more fun. PC vs. MAC in the beginning they don't even look they just don't know why it won't work. It's before they've had the chance for their 5th cup of coffee. For the techs - Setting the microtome to 0 on older ones or changing the auto to not advance. I had trouble with the automated ones but found I could rig it just right. If ever practice made perfect - this was one, because I learned alot on my own about automation there. Have fun and be creative. Lab work can be pretty intense - some good clean laughs break the intensity. On Wed, Mar 21, 2012 at 11:27 AM, Breeden, Sara sbree...@nmda.nmsu.eduwrote: I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's eyepieces. Vaseline on the coffee cup handles (don't all pathologists consume Mass Quantities of the stuff???). A tray of completely blank slides, all properly numbered for the day's work? Feed me some good ones, too. I'm down to my last week and I need to be remembered when I'm gone so they won't call me to do p.r.n.! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] April fool's prank
I second that. I hope you are kidding! Hm. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beckham, Sharon Sent: Wednesday, March 21, 2012 9:13 AM To: 'Kara Lee'; sbree...@nmda.nmsu.edu; tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank Please tell me you are kidding about all this stuff! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Wednesday, March 21, 2012 10:43 AM To: sbree...@nmda.nmsu.edu; tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank While I'm new at histology, I am a master of pranks. Here's a list of some fun ones I've done to co-workers over that years...that haven't gotten me fired or killed yet ;) 1. fill their desk space with packing peanuts 2. If you have the time, post-it note their entire office, office wall, ceiling and desk with the multi colored post-it notes...it's pretty and funny 3. Find a dead keyboard in the IT department. Take it home, fill the areas around the keys with dirt and chia-pet seeds. Wait for them to sprout. Come in early in the morning and replace someones keyboard with this one (don't actually plug it into the computer) 4. Pop out all the keys on the keyboard that spell out the persons user name...hide them around their desk. 5. Wait for them to walk away, change their monitor settings, then change the language to something they don't know so they don't know how to get it back to normal. 6. If they hate the smell of bananas, your lotion, etc., put a peel, open bottle, whatever hidden behind their desk. 7. Tilt their desk up just slightly in the back so all their pens roll off, but it's not obvious that the desk has been lifted. Enjoy! Kara Lee Date: Wed, 21 Mar 2012 09:27:11 -0600 From: sbree...@nmda.nmsu.edu To: tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank CC: histonet@lists.utsouthwestern.edu I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's eyepieces. Vaseline on the coffee cup handles (don't all pathologists consume Mass Quantities of the stuff???). A tray of completely blank slides, all properly numbered for the day's work? Feed me some good ones, too. I'm down to my last week and I need to be remembered when I'm gone so they won't call me to do p.r.n.! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] April fool's prank
Nope. a fun work environment breeds better workers. From: dstilli...@unipathdx.com To: s...@stowers.org; karabo...@hotmail.com; sbree...@nmda.nmsu.edu; tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu CC: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank Date: Wed, 21 Mar 2012 16:17:23 + I second that. I hope you are kidding! Hm. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beckham, Sharon Sent: Wednesday, March 21, 2012 9:13 AM To: 'Kara Lee'; sbree...@nmda.nmsu.edu; tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank Please tell me you are kidding about all this stuff! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Wednesday, March 21, 2012 10:43 AM To: sbree...@nmda.nmsu.edu; tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank While I'm new at histology, I am a master of pranks. Here's a list of some fun ones I've done to co-workers over that years...that haven't gotten me fired or killed yet ;) 1. fill their desk space with packing peanuts 2. If you have the time, post-it note their entire office, office wall, ceiling and desk with the multi colored post-it notes...it's pretty and funny 3. Find a dead keyboard in the IT department. Take it home, fill the areas around the keys with dirt and chia-pet seeds. Wait for them to sprout. Come in early in the morning and replace someones keyboard with this one (don't actually plug it into the computer) 4. Pop out all the keys on the keyboard that spell out the persons user name...hide them around their desk. 5. Wait for them to walk away, change their monitor settings, then change the language to something they don't know so they don't know how to get it back to normal. 6. If they hate the smell of bananas, your lotion, etc., put a peel, open bottle, whatever hidden behind their desk. 7. Tilt their desk up just slightly in the back so all their pens roll off, but it's not obvious that the desk has been lifted. Enjoy! Kara Lee Date: Wed, 21 Mar 2012 09:27:11 -0600 From: sbree...@nmda.nmsu.edu To: tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank CC: histonet@lists.utsouthwestern.edu I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's eyepieces. Vaseline on the coffee cup handles (don't all pathologists consume Mass Quantities of the stuff???). A tray of completely blank slides, all properly numbered for the day's work? Feed me some good ones, too. I'm down to my last week and I need to be remembered when I'm gone so they won't call me to do p.r.n.! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] April fool's prank
I'm with you Kara. No harm no foul. Lee Ann Baldridge IUSM Indpls., IN. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Wednesday, March 21, 2012 12:19 PM To: dstilli...@unipathdx.com; s...@stowers.org; sbree...@nmda.nmsu.edu; tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank Nope. a fun work environment breeds better workers. From: dstilli...@unipathdx.com To: s...@stowers.org; karabo...@hotmail.com; sbree...@nmda.nmsu.edu; tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu CC: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank Date: Wed, 21 Mar 2012 16:17:23 + I second that. I hope you are kidding! Hm. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beckham, Sharon Sent: Wednesday, March 21, 2012 9:13 AM To: 'Kara Lee'; sbree...@nmda.nmsu.edu; tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank Please tell me you are kidding about all this stuff! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Wednesday, March 21, 2012 10:43 AM To: sbree...@nmda.nmsu.edu; tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank While I'm new at histology, I am a master of pranks. Here's a list of some fun ones I've done to co-workers over that years...that haven't gotten me fired or killed yet ;) 1. fill their desk space with packing peanuts 2. If you have the time, post-it note their entire office, office wall, ceiling and desk with the multi colored post-it notes...it's pretty and funny 3. Find a dead keyboard in the IT department. Take it home, fill the areas around the keys with dirt and chia-pet seeds. Wait for them to sprout. Come in early in the morning and replace someones keyboard with this one (don't actually plug it into the computer) 4. Pop out all the keys on the keyboard that spell out the persons user name...hide them around their desk. 5. Wait for them to walk away, change their monitor settings, then change the language to something they don't know so they don't know how to get it back to normal. 6. If they hate the smell of bananas, your lotion, etc., put a peel, open bottle, whatever hidden behind their desk. 7. Tilt their desk up just slightly in the back so all their pens roll off, but it's not obvious that the desk has been lifted. Enjoy! Kara Lee Date: Wed, 21 Mar 2012 09:27:11 -0600 From: sbree...@nmda.nmsu.edu To: tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank CC: histonet@lists.utsouthwestern.edu I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's eyepieces. Vaseline on the coffee cup handles (don't all pathologists consume Mass Quantities of the stuff???). A tray of completely blank slides, all properly numbered for the day's work? Feed me some good ones, too. I'm down to my last week and I need to be remembered when I'm gone so they won't call me to do p.r.n.! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] April fool's prank
I'm loving it! Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beckham, Sharon Sent: Wednesday, March 21, 2012 12:13 PM To: 'Kara Lee'; sbree...@nmda.nmsu.edu; tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank Please tell me you are kidding about all this stuff! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Wednesday, March 21, 2012 10:43 AM To: sbree...@nmda.nmsu.edu; tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank While I'm new at histology, I am a master of pranks. Here's a list of some fun ones I've done to co-workers over that years...that haven't gotten me fired or killed yet ;) 1. fill their desk space with packing peanuts 2. If you have the time, post-it note their entire office, office wall, ceiling and desk with the multi colored post-it notes...it's pretty and funny 3. Find a dead keyboard in the IT department. Take it home, fill the areas around the keys with dirt and chia-pet seeds. Wait for them to sprout. Come in early in the morning and replace someones keyboard with this one (don't actually plug it into the computer) 4. Pop out all the keys on the keyboard that spell out the persons user name...hide them around their desk. 5. Wait for them to walk away, change their monitor settings, then change the language to something they don't know so they don't know how to get it back to normal. 6. If they hate the smell of bananas, your lotion, etc., put a peel, open bottle, whatever hidden behind their desk. 7. Tilt their desk up just slightly in the back so all their pens roll off, but it's not obvious that the desk has been lifted. Enjoy! Kara Lee Date: Wed, 21 Mar 2012 09:27:11 -0600 From: sbree...@nmda.nmsu.edu To: tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank CC: histonet@lists.utsouthwestern.edu I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's eyepieces. Vaseline on the coffee cup handles (don't all pathologists consume Mass Quantities of the stuff???). A tray of completely blank slides, all properly numbered for the day's work? Feed me some good ones, too. I'm down to my last week and I need to be remembered when I'm gone so they won't call me to do p.r.n.! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] April fool's prank
I guess everyone's idea of 'fun' can be different. A lot of these things sound like a lot of work to 'clean up'. Funny is different than making a mess to me--just 1 cent worth :-) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Wednesday, March 21, 2012 11:19 AM To: dstilli...@unipathdx.com; Beckham, Sharon; sbree...@nmda.nmsu.edu; tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank Nope. a fun work environment breeds better workers. From: dstilli...@unipathdx.com To: s...@stowers.org; karabo...@hotmail.com; sbree...@nmda.nmsu.edu; tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu CC: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank Date: Wed, 21 Mar 2012 16:17:23 + I second that. I hope you are kidding! Hm. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beckham, Sharon Sent: Wednesday, March 21, 2012 9:13 AM To: 'Kara Lee'; sbree...@nmda.nmsu.edu; tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank Please tell me you are kidding about all this stuff! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kara Lee Sent: Wednesday, March 21, 2012 10:43 AM To: sbree...@nmda.nmsu.edu; tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank While I'm new at histology, I am a master of pranks. Here's a list of some fun ones I've done to co-workers over that years...that haven't gotten me fired or killed yet ;) 1. fill their desk space with packing peanuts 2. If you have the time, post-it note their entire office, office wall, ceiling and desk with the multi colored post-it notes...it's pretty and funny 3. Find a dead keyboard in the IT department. Take it home, fill the areas around the keys with dirt and chia-pet seeds. Wait for them to sprout. Come in early in the morning and replace someones keyboard with this one (don't actually plug it into the computer) 4. Pop out all the keys on the keyboard that spell out the persons user name...hide them around their desk. 5. Wait for them to walk away, change their monitor settings, then change the language to something they don't know so they don't know how to get it back to normal. 6. If they hate the smell of bananas, your lotion, etc., put a peel, open bottle, whatever hidden behind their desk. 7. Tilt their desk up just slightly in the back so all their pens roll off, but it's not obvious that the desk has been lifted. Enjoy! Kara Lee Date: Wed, 21 Mar 2012 09:27:11 -0600 From: sbree...@nmda.nmsu.edu To: tajib...@echd.org; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] April fool's prank CC: histonet@lists.utsouthwestern.edu I always liked the M*A*S*H* one with the charcoal (?) on the 'scope's eyepieces. Vaseline on the coffee cup handles (don't all pathologists consume Mass Quantities of the stuff???). A tray of completely blank slides, all properly numbered for the day's work? Feed me some good ones, too. I'm down to my last week and I need to be remembered when I'm gone so they won't call me to do p.r.n.! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] commercial antibody diluent with carrier proteins SUGGESTIONS?
I like the DAKO antibody diluent. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2588 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joseph Madary Sent: Wednesday, March 21, 2012 8:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] commercial antibody diluent with carrier proteins SUGGESTIONS? Hi all, I would like to get a commercially prepared antibody diluent with carrier proteins. I have been told Dako has a decent one but I am trying to find a different vendor. I see a few places with diluent but none that have background reducing substances/carrier protein type thing. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 pat...@gwumc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Block Storage-another question
I know to hold them for 20 years, but where? At the lab where the blocks were processed or at the lab where the slides were read? Ann -Original Message- From: McMahon, Loralee A loralee_mcma...@urmc.rochester.edu To: Cynthia Pyse cp...@x-celllab.com; 'Rene J Buesa' rjbu...@yahoo.com; 'Ann Angelo' thisis...@aol.com; histonet histonet@lists.utsouthwestern.edu; 'FeltonNails' flna...@texaschildrens.org Sent: Wed, Mar 21, 2012 11:55 am Subject: RE: [Histonet] Block Storage-another question I would think that if NYS is involved in anyway you have to hold those blocks or 20 years. When they inspect you do they inspect both labs? Do both labs old a NYS permit? For cetain I would call NYS before you discard anything. Loralee McMahon, HTL (ASCP) mmunohistochemistry Supervisor trong Memorial Hospital epartment of Surgical Pathology 585) 275-7210 ___ rom: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] n Behalf Of Cynthia Pyse [cp...@x-celllab.com] ent: Wednesday, March 21, 2012 11:25 AM o: 'Rene J Buesa'; 'Ann Angelo'; histonet@lists.utsouthwestern.edu; FeltonNails' ubject: RE: [Histonet] Block Storage-another question If the pod lab is in NJ and the reading lab is in NY, which guide lines do ou follow. NYS requires us to save our blocks for 20 years. Due to storage ssues, I would rather the pod lab store the blocks. indy -Original Message- rom: histonet-boun...@lists.utsouthwestern.edu mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa ent: Wednesday, March 21, 2012 10:20 AM o: 'Ann Angelo'; histonet@lists.utsouthwestern.edu; FeltonNails ubject: RE: [Histonet] Block Storage Depending on the state regulations and the laboratory policies,blocks are tored during different periods of time before being discarded. I used to tore them for 9 years. ené J. --- On Wed, 3/21/12, Nails, Felton flna...@texaschildrens.org wrote: rom: Nails, Felton flna...@texaschildrens.org ubject: RE: [Histonet] Block Storage o: 'Ann Angelo' thisis...@aol.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu ate: Wednesday, March 21, 2012, 9:00 AM his is a very sticky issue, when I setup inhouse labs I always present the rgument that during inspections the lab that produces a report should have ccess to all test material which includes blocks and slides per the CAP hecklist. nn trying approaching it from that angle. -Original Message- rom: histonet-boun...@lists.utsouthwestern.edu mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Angelo ent: Tuesday, March 20, 2012 6:58 PM o: histonet@lists.utsouthwestern.edu ubject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they erform Technical component on if they do not perform the professional omponentor should they have the facility performing the professional omponent store them? Who is ultimately responsible? Ann __ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet __ ONFIDENTIALITY NOTICE: he information in this e-mail may be confidential and/or rivileged. If you are not the intended recipient or an uthorized representative of the intended recipient, you re hereby notified that any review, dissemination, or opying of this e-mail and its attachments, if any, or he information contained herein is prohibited. If you ave received this e-mail in error, please immediately otify the sender by return e-mail and delete this e-mail rom your computer system. Thank you. _ ___ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet __ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet __ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Block Storage-another question
Thank you for your input. We always go by the most strict regulating body which is NY and we save our blocks 20 years. Ann -Original Message- From: Cynthia Pyse cp...@x-celllab.com To: 'Rene J Buesa' rjbu...@yahoo.com; 'Ann Angelo' thisis...@aol.com; histonet histonet@lists.utsouthwestern.edu; 'FeltonNails' flna...@texaschildrens.org Sent: Wed, Mar 21, 2012 11:25 am Subject: RE: [Histonet] Block Storage-another question If the pod lab is in NJ and the reading lab is in NY, which guide lines do ou follow. NYS requires us to save our blocks for 20 years. Due to storage ssues, I would rather the pod lab store the blocks. indy -Original Message- rom: histonet-boun...@lists.utsouthwestern.edu mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa ent: Wednesday, March 21, 2012 10:20 AM o: 'Ann Angelo'; histonet@lists.utsouthwestern.edu; FeltonNails ubject: RE: [Histonet] Block Storage Depending on the state regulations and the laboratory policies,blocks are tored during different periods of time before being discarded. I used to tore them for 9 years. ené J. --- On Wed, 3/21/12, Nails, Felton flna...@texaschildrens.org wrote: rom: Nails, Felton flna...@texaschildrens.org ubject: RE: [Histonet] Block Storage o: 'Ann Angelo' thisis...@aol.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu ate: Wednesday, March 21, 2012, 9:00 AM his is a very sticky issue, when I setup inhouse labs I always present the rgument that during inspections the lab that produces a report should have ccess to all test material which includes blocks and slides per the CAP hecklist. nn trying approaching it from that angle. -Original Message- rom: histonet-boun...@lists.utsouthwestern.edu mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Angelo ent: Tuesday, March 20, 2012 6:58 PM o: histonet@lists.utsouthwestern.edu ubject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they erform Technical component on if they do not perform the professional omponentor should they have the facility performing the professional omponent store them? Who is ultimately responsible? Ann __ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet __ ONFIDENTIALITY NOTICE: he information in this e-mail may be confidential and/or rivileged. If you are not the intended recipient or an uthorized representative of the intended recipient, you re hereby notified that any review, dissemination, or opying of this e-mail and its attachments, if any, or he information contained herein is prohibited. If you ave received this e-mail in error, please immediately otify the sender by return e-mail and delete this e-mail rom your computer system. Thank you. _ ___ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet __ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fixatives for breast
What are the best fixatives for breast specimens? Also what the most commonly used fixatives out there? LaSalette ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Certified techs/high complexity testing
I would be very careful when you say you need a CLIA certified tech to do gross (high complexity testing). You actually need someone who meets the CLIA requirements to perform High Complexity Testing. You can have a tech who is certified, but they may not meet the educational requirements. That person MUST have 60 semester hours, 24 of those in science, 6 hours must be Biology and 6 must be Chemistry and documented training in the area of high complexity testing. It has NOTHING to with certification, unfortunately. Kelly D. Boyd, BS, HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: commercial antibody diluent with carrier proteins
I have used the Renaissance Background Reducing Diluent from Biocare Medical. It works great on human and rodent tissues. Biocare also had a wide selection of diluents and blocking reagents when used together can significantly reduce background and enhance staining intensity. Best Regards, Dave Investigative Pathology GlaxoSmithKline ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Certified techs/high complexity testing
Exactly, we are trying to educate our staff on this currently. It is important to understand because the regulatory bodies do focus on this issue. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd Sent: Wednesday, March 21, 2012 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Certified techs/high complexity testing I would be very careful when you say you need a CLIA certified tech to do gross (high complexity testing). You actually need someone who meets the CLIA requirements to perform High Complexity Testing. You can have a tech who is certified, but they may not meet the educational requirements. That person MUST have 60 semester hours, 24 of those in science, 6 hours must be Biology and 6 must be Chemistry and documented training in the area of high complexity testing. It has NOTHING to with certification, unfortunately. Kelly D. Boyd, BS, HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] April fool's prank
Amen!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryostat sectioning
Hi, We are searching for a fee for service lab experienced in cryostat sectioning of animal and human tissues. Any recommendation is appreciated. Thanks, Shu-Cheng Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Block Storage
Regardless where the blocks are generated, that private lab has to follow state regulations. That will be a legal matter related to any possible law suit after an alleged misdiagnosis. If the private lab has no available space, there are companies that can store blocks and slides for as long they are paid to retain them. René J. --- On Wed, 3/21/12, Nails, Felton flna...@texaschildrens.org wrote: From: Nails, Felton flna...@texaschildrens.org Subject: RE: [Histonet] Block Storage To: 'Rene J Buesa' rjbu...@yahoo.com, 'Ann Angelo' thisis...@aol.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, March 21, 2012, 11:19 AM Rene I think this question is eluding to a Physician owned lab and who is responsible for storing the blocks not how long they are retained? From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Wednesday, March 21, 2012 9:20 AM To: 'Ann Angelo'; histonet@lists.utsouthwestern.edu; Nails, Felton Subject: RE: [Histonet] Block Storage Depending on the state regulations and the laboratory policies,blocks are stored during different periods of time before being discarded. I used to store them for 9 years. René J. --- On Wed, 3/21/12, Nails, Felton flna...@texaschildrens.org wrote: From: Nails, Felton flna...@texaschildrens.org Subject: RE: [Histonet] Block Storage To: 'Ann Angelo' thisis...@aol.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, March 21, 2012, 9:00 AM This is a very sticky issue, when I setup inhouse labs I always present the argument that during inspections the lab that produces a report should have access to all test material which includes blocks and slides per the CAP checklist. Ann trying approaching it from that angle. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Angelo Sent: Tuesday, March 20, 2012 6:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they perform Technical component on if they do not perform the professional componentor should they have the facility performing the professional component store them? Who is ultimately responsible? Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Block Storage-another question
Both but especially the place where the diagnosis takes place. René J. --- On Wed, 3/21/12, Cynthia Pyse cp...@x-celllab.com wrote: From: Cynthia Pyse cp...@x-celllab.com Subject: RE: [Histonet] Block Storage-another question To: 'Rene J Buesa' rjbu...@yahoo.com, 'Ann Angelo' thisis...@aol.com, histonet@lists.utsouthwestern.edu, 'FeltonNails' flna...@texaschildrens.org Date: Wednesday, March 21, 2012, 11:25 AM If the pod lab is in NJ and the reading lab is in NY, which guide lines do you follow. NYS requires us to save our blocks for 20 years. Due to storage issues, I would rather the pod lab store the blocks. Cindy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, March 21, 2012 10:20 AM To: 'Ann Angelo'; histonet@lists.utsouthwestern.edu; FeltonNails Subject: RE: [Histonet] Block Storage Depending on the state regulations and the laboratory policies,blocks are stored during different periods of time before being discarded. I used to store them for 9 years. René J. --- On Wed, 3/21/12, Nails, Felton flna...@texaschildrens.org wrote: From: Nails, Felton flna...@texaschildrens.org Subject: RE: [Histonet] Block Storage To: 'Ann Angelo' thisis...@aol.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, March 21, 2012, 9:00 AM This is a very sticky issue, when I setup inhouse labs I always present the argument that during inspections the lab that produces a report should have access to all test material which includes blocks and slides per the CAP checklist. Ann trying approaching it from that angle. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Angelo Sent: Tuesday, March 20, 2012 6:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Storage Does anyone know if a laboratory in NJ is required to keep the blocks they perform Technical component on if they do not perform the professional componentor should they have the facility performing the professional component store them? Who is ultimately responsible? Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Fixatives for breast
Neutral buffered formalin for 36 hours at room temperature. René J. --- On Wed, 3/21/12, LaSalette Charette las.chare...@comcast.net wrote: From: LaSalette Charette las.chare...@comcast.net Subject: [Histonet] Fixatives for breast To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, March 21, 2012, 12:59 PM What are the best fixatives for breast specimens? Also what the most commonly used fixatives out there? LaSalette ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] antigen retrieval
To those with the Biocare intelliPATH system, Is the antigen retrieval part of the IHC automated or do you need to use the Biocare decloaker? Thankyou, Tom Truscott ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] commercial antibody diluent with carrier proteins SUGGESTIONS?
Joseph: ALL commercial antibody diluent carriers contain the same ingredients (bovine albumen, Tween 20 and sodium azide as preservative in pH7 PBS buffer). Every 100 mL of antibody diluent contains: Bovine albumen - 1 mL PBS pH7 buffer - 99 mL Tween 20 - 50 µL sodium azide --100 mg Why don't you prepare it yourself? That is what I used to do and saved a lot of money! René J. --- On Wed, 3/21/12, Joseph Madary pat...@gwumc.edu wrote: From: Joseph Madary pat...@gwumc.edu Subject: [Histonet] commercial antibody diluent with carrier proteins SUGGESTIONS? To: histonet@lists.utsouthwestern.edu Date: Wednesday, March 21, 2012, 11:27 AM Hi all, I would like to get a commercially prepared antibody diluent with carrier proteins. I have been told Dako has a decent one but I am trying to find a different vendor. I see a few places with diluent but none that have background reducing substances/carrier protein type thing. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 pat...@gwumc.edu -Inline Attachment Follows- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: antigen retrieval
Tim- Antigen retrieval is off-line... you need the decloaker or other device. Brett M. Connolly, Ph.D. Imaging Research Fellow Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: Wednesday, March 21, 2012 4:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antigen retrieval To those with the Biocare intelliPATH system, Is the antigen retrieval part of the IHC automated or do you need to use the Biocare decloaker? Thankyou, Tom Truscott ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Block Storage-another question
Fellow Histonet Subscribers, I have been noticing a LOT of misinformation being passed around this site, much coming from one respondent in particular. This is very troubling, as our position in healthcare, and the work we do, is so very critical to patient care and patient outcomes. I would hope that before responding to questions, people either know what they are talking about, or make it clear that it is opinion they are expressing, and not necessarily fact. Perhaps including some reference, or web link to information is practical at times. Here I have listed below responses to some questions that are outright false, and the information presented, if followed, in some instances may put a lab in jeopardy when inspected. It also appears that we have techs from marine biology centers, and plant histology labs answering questions pertaining to medicine. I am not sure how appropriate that is. This is, of course, just my opinion. *In Response to the question of where to store blocks this was an answer:* “Both but especially the place where the diagnosis takes place.” It is not possible to store blocks in BOTH locations. Why would you do that? And “especially the place where the diagnosis takes place” is incorrect. The logical place to keep the blocks is where the histology is performed. If space does not permit long term storage, rent a facility that is compliant with your state requirements for doing so. As far as time to keep blocks, CLIA and CAP have different requirements. Check with your State to see if they are even more stringent than CAP. Here is what CLIA, and CAP require: FROM CLIA: Sec. 493.1105 Standard: Retention requirements (a)(3)(ii) Blocks. Retain pathology specimen blocks for at least 2 years from the date of examination. FROM CAP: Retention of Laboratory Records and Materials: *Surgical Pathology (including bone marrows)* Wet tissue 2 weeks after final report Paraffin blocks 10 years Slides 10 years Reports 10 years *In Response to Water Quality this response was noted:* “No small laboratory has the conditions required to perform microbiology cultures” This is an inaccurate statement. Size of a lab, and ability to perform work in a sterile environment are not at all related. When responding to questions as important as breast fixation times, it would be helpful to include information like what I included immediately below – furthermore, as all of you know, breast tissue fixes differently depending upon several factors, especially fat content, and thickness of the sections. The question of best fixation time is not an answerable question – it is specific to the case itself. Is it cores we are talking about? Is it a dense fibrous lumpectomy? Is it a fatty mastectomy? There is no perfect answer to that question. *What are the changes made to minimum fixation times?* The minimum fixation time for HER2 has been clarified and we recommend that samples for HER2 testing be fixed a minimum of 6 hours. The original statement that smaller samples can be fixed for less than 6 hours is not supported by the literature. We recommend that sample for HER2 testing be fixed a minimum of 6 hours regardless of sample size. *WWhat about changes to maximum fixation times? The HER2 fixation time of 6-48 hours is not consistent with that of the ER/PgR fixation time of 6-72 hours.* We are unable to find evidence to support increasing the HER2 fixation time and therefore recommendations for fixation times in neutral buffered formalin are unchanged (6-48 hours for HER2 and 6-72 hours for ER/PgR). The data about the stability of ER and PgR at intervals of 48-72 hours suggest that changing this interval for HER2 testing will not result in adverse testing results. However, there is a lack of specific published studies for HER2 IHC that included specimens with low levels of HER2 expression that would be more vulnerable to fixation time changes. *What are the changes made to minimum fixation times?* The minimum fixation time for HER2 has been clarified and we recommend that samples for HER2 testing be fixed a minimum of 6 hours. The original statement that smaller samples can be fixed for less than 6 hours is not supported by the literature. We recommend that sample for HER2 testing be fixed a minimum of 6 hours regardless of sample size. On Wed, Mar 21, 2012 at 1:27 PM, Rene J Buesa rjbu...@yahoo.com wrote: Both but especially the place where the diagnosis takes place. René J. --- On Wed, 3/21/12, Cynthia Pyse cp...@x-celllab.com wrote: From: Cynthia Pyse cp...@x-celllab.com Subject: RE: [Histonet] Block Storage-another question To: 'Rene J Buesa' rjbu...@yahoo.com, 'Ann Angelo' thisis...@aol.com, histonet@lists.utsouthwestern.edu, 'FeltonNails' flna...@texaschildrens.org Date: Wednesday, March 21, 2012, 11:25 AM If the pod lab is in NJ and the reading lab is in NY, which guide lines do you follow. NYS requires us to save our blocks for
[Histonet] Job Opening orange county
We have a part-time histotech position available and anyone who is interested, please send me your resume by fax or email. The work days are Tuesday through Saturday starting at 5 am. Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA 92708 Fax: 714-755-2984 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: April fool's prank
Not to put a damper on your fun but something to think about. I have played my April fools pranks and also been at the other end on many occasions. Let us not forget in these high stress times that perceptions vary considerably and what one person considers as a harmless and amusing prank to them may be embarrassing or even mentally damaging to another. If you have seen Revenge of the Geeks with its team bullying you know what I mean. Please think long and hard before you start any pranks. Barry From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tunde Ajibade [tajib...@echd.org] Sent: Wednesday, March 21, 2012 10:16 AM To: 'histonet-boun...@lists.utsouthwestern.edu' Cc: histonet@lists.utsouthwestern.edu Subject: [Histonet] April fool's prank Any ideal for April fool's prank? Tunde Ajibade BS, HTL(ASCP)QIHC CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: april fools prank
I've been known to leave a fake spider in an embedder for the morning person.although it wasn't april fools just for fun it got a great reaction :) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
