[Histonet] Job opening Gaithersburg,MD

[Lila Adams]

Anyone interested in Histology job?

 

CV Path Institute, Inc is a laboratory based in Gaithersburg, MD focused
primarily on cardiovascular medical research and pre-clinical studies seeks
a full-time Histotechnician. Candidate must have expertise in embedding,
microtomy, routine and special staining In paraffin sections. HT (ASCP)
certification or HT eligible is preferred. Medical insurance coverage and
retirement package include as benefit. Please send resume/CV with salary
requirements to dh...@cvpath.org .

 

Lila R. Adams

IHC Research Lab

CVPath Institute, Inc

19 Firstfield Road

Gaithersburg, MD 20878

301-208-3570 ext 138

 

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[Histonet] 2012 Region II Symposium 26-28 Apr.2012, Williamsburg, VA

[Lila Adams]

Hello to Histonetters ,

 

Just wanted to let you know there's a Region II Symposium on 26-28 April
2012 in Williamsburg, Virginia. There will be a choice of 18 to 19
workshops/lectures being offered during the three days. Fees are $50.00 for
Thursday, $100.00 for Friday, $80.00 for Saturday or $200.00 for all three
days. CEU's will be offered for most workshops and lectures. Go to the
nsh.org/calendar and click on  Region II Symposium for the entire brochure.
If you have questions concerning registration contact Michelle Hart
302-733-4709 or Elaine Perkins 302-733-3659. The symposium fees include
continental breakfast, breaks, lunch and a reception on Friday 27th April
2012. Come join in the Fun!

 

 

Lila R. Adams

IHC Research Lab

CVPath Institute, Inc

19 Firstfield Road

Gaithersburg, MD 20878

301-208-3570 ext 138

 

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RE: [Histonet] Thank you

[Patsy Ruegg]

The best markers I have ever used are called KP Markers, they were off the
market for a while, but they are back and we get them from Mercedes Medical,
just got a new batch and they are wonderful just like the old KP markers, we
won't have anything else in the lab.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock
Sent: Wednesday, April 11, 2012 2:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Thank you

Hi Everyone:

I want to thank everyone who gave me advice concerning my cassette 
labeling problem.
I am trying out three different types of markers pens: StatLab, Leica, 
and NewComer Supply Histo-tec brands.
We will also make sure to let the ink dry before putting the cassettes 
into formalin.
If we still have a problem, we will experiment with a different cassette.
Thanks again.

Tim Wheelock
Harvard Brain Bank
McLean Hospital
Belmont, MA
617-855-3592

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Re: [Histonet] Sections of poly(caprolactone) based scaffolds washing off the slide

[Richard Thibault]

Sorry, for clarification I meant the PCL isn't* adherent to the slide and
way too* harsh

On Wed, Apr 11, 2012 at 16:41,  wrote:

> Dear Histonet,
>
> Sorry in advance for the long email.  I would like to ask for advice
> regarding some of my sections rinsing off my slides.  I generate composite
> tissue engineered electrospun poly(caprolactone) (PCL) and cell-generated
> extracellular matrix (ECM) scaffolds. With larger amount of ECM coated
> around the PCL, I have no issues of retaining the entire section.  However,
> with minimal ECM coating, the PCL portion of the section is easily washed
> off.  The PCL portion of the scaffold is a porous electrospun random fiber
> mesh with fiber diameters of 10 microns.  The method I?ve tried is detailed
> below.
>
> 1. Fix the composite scaffold in 2.5% glutaraldehyde for 45 minutes.
> 2. Soak in Histoprep overnight at 4 C to ensure distribution of the
> Histoprep throughout the pores of the scaffold.
> 3. Embed the scaffold and freeze into blocks.
> 4. Let the embedded scaffold sit in the -80 C freezer overnight then
> transfer to the -20 C freezer. (this helps with the sectioning of our
> scaffolds, better integration of the scaffold with the rest of the
> Histoprep)
> 5. Cryosection into 5 micron sections and place onto superfrost plus
> slides.
>
> After this I run into problems.  I?ve kept the slides at -20 C and let
> them warm to room temp before staining with picrosirus red or Safranin O
> etc. as well as baking them on the slide warmer at 45 C for 1 day and up to
> a week.  I?ve also tried using superfrost excell slides, poly(L-lysine)
> coated slides, unaltered glass slides, and silanized slides (I didn?t
> expect it to work since the embedding medium is hydrophilic and rightly so,
> the entire section just beaded up destroying the section).  Each of these
> processes resulted in the PCL portion of the scaffold rinsing off within 2
> or 3 steps of the stain except for the 7 day baking step, which retained
> parts but not all of the PCL portion.
>
> For the staining procedure, I outline the section with a Dako pen, then
> drip the water, stains, etc. into the outline but not on the scaffold
> itself using a transfer pipet until everything is covered.  To remove the
> liquid, I use a pipettor set to 200 microliters to aspirate, again away
> from the section/scaffold, but since the PCL is adherent to the slide, it
> gets aspirated off along with the water.  Using an autostainer is way to
> harsh for these scaffolds, so I reverted to careful manual staining.
>
> Additionally, due to the properties of the PCL, I can?t embed using
> paraffin since the melting point of PCL is 60 C and PCL dissolves in
> xylene, chloroform, acetone, DMF, and THF.  I also can?t use gelatin coated
> slides since I need to stain for the collagen in the ECM coating.
>
> Does anyone have any insight to help retain the PCL portion of the
> scaffolds?  Steps I could take, different slides that may work better?  I
> do know that PCL becomes hydrophilic if treated with a high normal NaOH
> (i.e. the ester bonds in the polymer breaks down to carboxylic groups), but
> I?m not sure if that could work, since if I perform the NaOH treatment
> before sectioning only the outside of the fibers will become hydrophilic
> and if I do it after sectioning, it wouldn?t affect the bottom portion of
> the section.  This could also damage the ECM as well.  I can upload
> pictures of before and after staining if it would help. I would appreciate
> any help you could suggest.
>
> Thanks,
>
> Rich
>
>
>
> __**_
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> http://lists.utsouthwestern.**edu/mailman/listinfo/histonet
>
>
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[Histonet] Sections of poly(caprolactone) based scaffolds washing off the slide

[rat1]

Dear Histonet,

Sorry in advance for the long email.  I would like to ask for advice  
regarding some of my sections rinsing off my slides.  I generate  
composite tissue engineered electrospun poly(caprolactone) (PCL) and  
cell-generated extracellular matrix (ECM) scaffolds. With larger  
amount of ECM coated around the PCL, I have no issues of retaining the  
entire section.  However, with minimal ECM coating, the PCL portion of  
the section is easily washed off.  The PCL portion of the scaffold is  
a porous electrospun random fiber mesh with fiber diameters of 10  
microns.  The method I?ve tried is detailed below.


1. Fix the composite scaffold in 2.5% glutaraldehyde for 45 minutes.
2. Soak in Histoprep overnight at 4 C to ensure distribution of the  
Histoprep throughout the pores of the scaffold.

3. Embed the scaffold and freeze into blocks.
4. Let the embedded scaffold sit in the -80 C freezer overnight then  
transfer to the -20 C freezer. (this helps with the sectioning of our  
scaffolds, better integration of the scaffold with the rest of the  
Histoprep)

5. Cryosection into 5 micron sections and place onto superfrost plus slides.

After this I run into problems.  I?ve kept the slides at -20 C and let  
them warm to room temp before staining with picrosirus red or Safranin  
O etc. as well as baking them on the slide warmer at 45 C for 1 day  
and up to a week.  I?ve also tried using superfrost excell slides,  
poly(L-lysine) coated slides, unaltered glass slides, and silanized  
slides (I didn?t expect it to work since the embedding medium is  
hydrophilic and rightly so, the entire section just beaded up  
destroying the section).  Each of these processes resulted in the PCL  
portion of the scaffold rinsing off within 2 or 3 steps of the stain  
except for the 7 day baking step, which retained parts but not all of  
the PCL portion.


For the staining procedure, I outline the section with a Dako pen,  
then drip the water, stains, etc. into the outline but not on the  
scaffold itself using a transfer pipet until everything is covered.   
To remove the liquid, I use a pipettor set to 200 microliters to  
aspirate, again away from the section/scaffold, but since the PCL is  
adherent to the slide, it gets aspirated off along with the water.   
Using an autostainer is way to harsh for these scaffolds, so I  
reverted to careful manual staining.


Additionally, due to the properties of the PCL, I can?t embed using  
paraffin since the melting point of PCL is 60 C and PCL dissolves in  
xylene, chloroform, acetone, DMF, and THF.  I also can?t use gelatin  
coated slides since I need to stain for the collagen in the ECM coating.


Does anyone have any insight to help retain the PCL portion of the  
scaffolds?  Steps I could take, different slides that may work better?  
 I do know that PCL becomes hydrophilic if treated with a high normal  
NaOH (i.e. the ester bonds in the polymer breaks down to carboxylic  
groups), but I?m not sure if that could work, since if I perform the  
NaOH treatment before sectioning only the outside of the fibers will  
become hydrophilic and if I do it after sectioning, it wouldn?t affect  
the bottom portion of the section.  This could also damage the ECM as  
well.  I can upload pictures of before and after staining if it would  
help. I would appreciate any help you could suggest.


Thanks,

Rich



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[Histonet] Thank you

[Tim Wheelock]

Hi Everyone:

I want to thank everyone who gave me advice concerning my cassette 
labeling problem.
I am trying out three different types of markers pens: StatLab, Leica, 
and NewComer Supply Histo-tec brands.
We will also make sure to let the ink dry before putting the cassettes 
into formalin.

If we still have a problem, we will experiment with a different cassette.
Thanks again.

Tim Wheelock
Harvard Brain Bank
McLean Hospital
Belmont, MA
617-855-3592

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Re: [Histonet] Looking for protzoal antibodies

[Mehmet Fatih BOZKURT]

Hello, there is antibody of neospora caninum in VMRD  http://www.vmrd.com/
.. I didnt use but i have heard that is good working.

for sarcocyst I can send blocks by regular mail. But Imho no need IHC for
sarcocyst.

On Wed, Apr 11, 2012 at 11:10 PM, Cynthia Hutchinson wrote:

> Hello All,
>
> Does anyone know of a source for clean Neospora caninum and/or Sarcocystis
> antibodies?
> Also, does anyone have any positive control tissue for toxo, neo, and/or
> sarco?
>
> Thank you,
> Cindy
>
> Cynthia Tily Hutchinson
> Rsch Asst IV
> Pathobiology
> Coll of Vet Med
> Auburn University
> Ph: 334-844-7020
> Fax: 334-844-2652
>
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>



-- 
Mehmet Fatih BOZKURT, DVM, PhD
Afyon Kocatepe University
Faculty of Veterinary Medicine
Department of Pathology
03030, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-109
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Re: [Histonet] Looking for protzoal antibodies

[Jan Shivers]

I get my Neospora caninum from VMRD; it's a goat polyclonal, but you can
dilute it way out so there's no background (currently using 1:24,000).

I may have a Toxo positive block for you.

Jan Shivers
IHC/Histo/EM Section Head
Veterinary Diagnostic Lab
UMN College of Veterinary Medicine
St. Paul, MN

On Wed, Apr 11, 2012 at 3:10 PM, Cynthia Hutchinson wrote:

> Hello All,
>
> Does anyone know of a source for clean Neospora caninum and/or Sarcocystis
> antibodies?
> Also, does anyone have any positive control tissue for toxo, neo, and/or
> sarco?
>
> Thank you,
> Cindy
>
> Cynthia Tily Hutchinson
> Rsch Asst IV
> Pathobiology
> Coll of Vet Med
> Auburn University
> Ph: 334-844-7020
> Fax: 334-844-2652
>
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>
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[Histonet] Looking for protzoal antibodies

[Cynthia Hutchinson]

Hello All,

Does anyone know of a source for clean Neospora caninum and/or Sarcocystis 
antibodies?
Also, does anyone have any positive control tissue for toxo, neo, and/or sarco?

Thank you,
Cindy

Cynthia Tily Hutchinson
Rsch Asst IV
Pathobiology
Coll of Vet Med
Auburn University
Ph: 334-844-7020
Fax: 334-844-2652

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[Histonet] HIPAA audit

[Lester Raff MD]

Hello All:

 

Do any of you have experience with internal HIPAA audits or monitoring,
as required by CAP checklist question Gen.41303?  We have a HIPAA policy
but have never done an audit.

 

 

 

Lester J. Raff, MD

Medical Director

UroPartners Laboratory

2225 Enterprise Dr. Suite 2511

Westchester, Il 60154

Tel 708.486.0076

Fax 708.492.0203

 

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RE: [Histonet] Slide disposal

[Wanda.Smith]

We package ours up to be incinerated.  Not so much because of biohazards, but 
our patient's names are on all the slides.
Thanks,
Wanda

WANDA G. SMITH, HTL(ASCP)HT 
Pathology Supervisor 
TRIDENT MEDICAL CENTER 
9330 Medical Plaza Drive 
Charleston, SC  29406 
843-847-4586 
843-847-4296 fax 

This email and any files transmitted with it may contain PRIVILEGED or 
CONFIDENTIAL information and may be read or used only by the intended 
recipient. If you are not the intended recipient of the email or any of its 
attachments, please be advised that you have received this email in error and 
that any use, dissemination, distribution, forwarding, printing, or copying of 
this email or any attached files is strictly prohibited. If you have received 
this email in error, please immediately purge it and all attachments and notify 
the sender by reply email or contact the sender at the number listed.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
indytreeg...@sbcglobal.net
Sent: Wednesday, April 11, 2012 10:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide disposal

Can anyone tell me the proper way to dispose of slides?  Are they considered 
biohazardous waste? 
 
Thanks so much, 
Lyn
 
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[Histonet] CSH 2012 Symposium-San Diego CA

[dusko trajkovic]

“Come Ride the Waves of Innovation”
 
This year’s meeting will be at the Bahia resort hotel on Mission Bay.  2 Blocks 
from the Ocean.  We are Presently lining up an outstanding list of lecturers.  
Classes will be held on 2 paddle wheel boats.  Take a look at the hotel website 
and start planning now.  Details and registration will be posted soon on the 
Society website.
 
http://www.bahiahotel.com/
http://www.californiahistology.org/events.html
 
The 2012 Symposium/Convention will be held in May in San Diego, CA.
When: May 3 - 6, 2012
Hotel Information:
Bahia Resort Hotel
998 West Mission Bay Drive, San Diego CA 858.488.0551 Reserve your room now at: 
https://shop.evanshotels.com/bahia_groups/casocf1205093.html
 
Note: To receive the group rate of $109.00 per night please mention CSH when 
registering with the hotel. The cutoff date for the group rate is April 3, 
2012.  We have already met our room number minimum, but more rooms are 
available 
at the discount rate.  Register and book you room soon.
 
We still have a few vendor tables available, but they are filling fast.
 
 
James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation
Tel    858-332-4647
Fax   858-812-1915
jwat...@gnf.org
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[Histonet] Aetna in-house

[Nicole Tatum]

I have reviewed my letter from Aetna and it reads," This change is
consistent with the Center for Medicare & Medicaid Services recongnition
of CAP as an approved accreditation organization for non-hospital anatomic
pathology testing."

I have called Medicare and they state that they have made no changes and
CLIA is the only enitity they require to recieve reimbursment for path
services reguardless of location. If that was the case I would definitly
have to get my CAP. Because if Medicare does, it you know they will all
follow suit.

So, to any smaller lab. Medicare has not changed to CAP and CLIA remains
the only certification you need.


Nicole Tatum HT ASCP


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Re: [Histonet] In House Labs in WSJ

[Nicole Tatum]

Agreed , but the idea in recent health care has been early detection. So
as technology increased, more diagnostic test were ordered. But, that is
not only pathology, its micro, radiology, ultrasound, chemistry, etc.
These early test did drive cost up, but also saved cost. Its kinda no win.
If a radical tumor was detected early it could be removed by surgery and
the patient could possibly still live a healthy cancer free life. If not
detected, the patient could suffer through chemo and expensive radiation,
and expensive hospice. Leaving the family devistated with medical bills
and the loss of a loved one that a simple diagnostic test could have
detected. Also, there is a huge problem with the malpractice suits in our
country. If the pateint did suffer or die because a simple test was not
ordered that could save their life the physician is held responsible and
sued and could possibly lose his license and career. If less test are the
answer to cutting cost to our rising health deficit, then doctors should
be more protected when they make choices not to order tests that could
save your life. Despite cost, in court they will argue it was a "simple"
inexpensive test that could have saved his or her life. The physician is
charged with protecting a patients health and he needs tool to do that.
Tools that are being taken because they are unaffordable. We must learn
how to manage our resources at every level. I for one would be devistated
if I had cervical cancer because my OB did not submit a specimen when my
pap came back as abnormal. I would be willing to pay the path fee out of
pocket to have an answer. But, that's also part of the problem. People do
not want to pay for services they recieve. But, they have a really nice
flat screen and iphone. This economic crisis is a result of the public and
health professional and gas prices, etc. We must stick together and come
up with ways to still use diagnostic test effectively. They do save lives
and save money, maybe on a small scale compared to those who are not
diagnosed with any condition. Our current health care model has been based
on detection and prevention. It will have to change for our industry to
survive. Resouces will have to be rationed but I fear it is being given
the title of over-utilization instead. As current tests decrease and
physician are pushed to order less; I fear there will be an increase of
misdiagnosises and an increase in malpractice suits. Its becomming scarry
out there.. This change will effect each one of us.

Nicole Tatum HT ASCP





 On 4/10/2012 5:33 PM, Kim Donadio wrote:
>>> Less screening = fewer biopsies = less revenue = less prostate cancers
>>> caught early = more deaths to prostate cancers.
>> Would you not agree?
>
> No. There is very good scientific evidence that screening does not
> increase survival rates but it does drive up costs and unnecessary
> surgery and related complications.
> I can send the papers from NEJM if you like.
>
> Geoff
>
>>
>> And for all those advocating closure of private labs, do you also feel
>> the same way about private pathologist owned labs who reep the benefits
>> of getting all the out PT work from affiliated physicians while they
>> also get a fee to serve as medical directors of hospital labs and get
>> the pc portion of hospital work of which they can order as many test
>> they want so they get the pc portion while the hospital gets the tc and
>> all the big bills associated with doing the test making it hard on tax
>> payer as well because so much in a hospital is already subsidize by the
>> gov.
>>
>> Is what you really want is to have all pathologist as employees of the
>> hospitals? And have the hospital bill global.
>>
>> And a few walmart like reference labs
>>
>> I'm just curious as to the exact position of some on here.
>>
>> Thanks
>>
>> Kim
>> Sent from my iPhone
>>
>> On Apr 10, 2012, at 2:39 PM, "Morken,
>> Timothy"  wrote:
>>
>>> Not surprising since our health care system is biased to pay for tests
>>> and treatments, not results. On top of this there are serious questions
>>> as to whether the PSA screening that leads to biopsies is useful in the
>>> long term. There is a recommendation out there to stop PSA screening
>>> for most men since it is largely  non-specific. That test is what leads
>>> to the biopsies. Less screening = fewer biopsies = less revenue.
>>>
>>> Tim Morken
>>>
>>>
>>>
>>> -Original Message-
>>> From: histonet-boun...@lists.utsouthwestern.edu
>>> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel
>>> Schneider
>>> Sent: Tuesday, April 10, 2012 11:22 AM
>>> To: Histonet
>>> Subject: [Histonet] In House Labs in WSJ
>>>
>>> The Wall Street Journal served up a timely article for us.
>>> You'll see both sides of the argument below. One side is right.
>>>
>>> DLS
>>>
>>> HEALTH INDUSTRY
>>> April 9, 2012, 7:22 p.m. ET
>>> Prostate-Test Fees Challenged
>>>
>>> By CHRISTOPHER WEAVER
>>> Doctors in urology groups that profit from tests

[Histonet] Slide disposal

[indytreegers]

Can anyone tell me the proper way to dispose of slides?  Are they considered 
biohazardous waste? 
 
Thanks so much, 
Lyn
 
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Re: [Histonet] New to paraffin cutting - seeking advice

[Marc DeCarlo]

Merissa,

I can't help with your paraffin needs, but have a suggestion for your
plastic.  Try Weigerts Hematoxylin.  It's available commercially through
several vendors and in my experience stains just like what your looking for.

If you need help just contact me.

Marc

On Monday, April 9, 2012, M.O. wrote:

> Hello everyone! I have had such wonderful feedback regarding plastic
> embedded specimens and now I am moving onto paraffin.  The majority of the
> specimens I am going to be cutting are paraffin embedded decal rabbit
> femurs.  I am using a Leica RM2255 and there is an option for retraction
> (after each section) and blade angle.  I need to practice a lot because
> right now my sections are crinkly even though I have my samples on ice, but
> hopefully practice makes perfect!
>
> So I want to ask you all a few questions regarding settings and technique
> and one about H&E staining.  What angle should the blade be at - I have it
> set at 0 for the moment.  What about the retraction option - I have it set
> at 15um.  The sections are being cut at 4um.
>
> When the blade has pieces of paraffin on it, do I need to clean that off?
> How do I remove this paraffin because I don't want to dull the blade? If
> not, does that impact the section quality and should I be moving to a
> different area of the blade?
>
> I am looking into flotation baths that are relatively inexpensive.  In
> particular, I am looking for a simple bath with a glass pyrex dish.  Do you
> have any suggestions on where to purchase one from?
>
> Lastly, I am going back to H&E staining of plastic embedded undecalcified
> bone, specifically hematoxylin, for a brief moment.  When I use a clearing
> solution I know that I am trying to destain a bit from the undecalcified
> bone to make a lighter stain, but does this also significantly destain the
> nuclei?  Right now, I need to leave the sample staining in Harris's
> hematoxylin for more than 4 mins to get the nuclei stained nicely, but I
> need to destain because the section is too dark. I just don't want to
> destain the nuclei too much, but want a lighter stain on the undecal bone.
>
> Again, thank you all for your support and advice, it is much appreciated!
>
> Sincerely,
> Merissa
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Re: [Histonet] In House Labs in WSJ

[Geoff McAuliffe]

On 4/10/2012 5:33 PM, Kim Donadio wrote:

Less screening = fewer biopsies = less revenue = less prostate cancers caught 
early = more deaths to prostate cancers.

Would you not agree?


No. There is very good scientific evidence that screening does not 
increase survival rates but it does drive up costs and unnecessary 
surgery and related complications.

I can send the papers from NEJM if you like.

Geoff



And for all those advocating closure of private labs, do you also feel the same 
way about private pathologist owned labs who reep the benefits of getting all 
the out PT work from affiliated physicians while they also get a fee to serve 
as medical directors of hospital labs and get the pc portion of hospital work 
of which they can order as many test they want so they get the pc portion while 
the hospital gets the tc and all the big bills associated with doing the test 
making it hard on tax payer as well because so much in a hospital is already 
subsidize by the gov.

Is what you really want is to have all pathologist as employees of the 
hospitals? And have the hospital bill global.

And a few walmart like reference labs

I'm just curious as to the exact position of some on here.

Thanks

Kim
Sent from my iPhone

On Apr 10, 2012, at 2:39 PM, "Morken, Timothy"  
wrote:


Not surprising since our health care system is biased to pay for tests and 
treatments, not results. On top of this there are serious questions as to 
whether the PSA screening that leads to biopsies is useful in the long term. 
There is a recommendation out there to stop PSA screening for most men since it 
is largely  non-specific. That test is what leads to the biopsies. Less 
screening = fewer biopsies = less revenue.

Tim Morken



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Schneider
Sent: Tuesday, April 10, 2012 11:22 AM
To: Histonet
Subject: [Histonet] In House Labs in WSJ

The Wall Street Journal served up a timely article for us.
You'll see both sides of the argument below. One side is right.

DLS

HEALTH INDUSTRY
April 9, 2012, 7:22 p.m. ET
Prostate-Test Fees Challenged

By CHRISTOPHER WEAVER
Doctors in urology groups that profit from tests for prostate cancer order more 
of them than doctors who send samples to independent laboratories, according to 
a study Monday in the journal Health Affairs.

The study found that doctors' practices that do their own lab work bill the 
federal Medicare program for analyzing 72% more prostate tissue samples per 
biopsy while detecting fewer cases of cancer than counterparts who send 
specimens to outside labs.

Hiring pathologists boosts revenue for a practice and creates a potential 
incentive to increase the number of tests ordered, said Jean Mitchell, a 
Georgetown University economist and author of the study.

That fewer cancers were detected-21% versus 35% for those sent to external labs, 
according to the study-suggests "financial incentives"
may play a role in decisions to order the tests, Ms. Mitchell said.

Some urologists said the research doesn't necessarily indicate financial 
motives. Urologists in larger group practices that have in-house pathologists 
may be more aggressive in testing because they seek to catch cancer earlier, 
said Steven Schlossberg, a Yale urologist who heads a health-policy panel for 
the American Urological Association and wasn't involved in the research. Also, 
Dr. Schlossberg noted, the figures, which cover 36,261 biopsies from 2005 
through 2007, are five years old.

The study was financed by the College of American Pathologists and the American 
Clinical Laboratory Association. It is the last salvo in a turf war between 
laboratory companies and physician groups that have opened their own labs to 
conduct tests.

Regulators and economists scrutinizing the growing costs of health care have 
targeted a range of related activities by doctors, known as self-referrals.

Although a set of 1990s-era laws, named for their proponent, Rep. Pete Stark 
(D., Calif.), ban doctors from referring patients to most companies in which 
they have a financial interest, urology groups can enter the pathology business 
because of an exemption for certain services performed within physicians' 
offices. The pathologists and other groups are lobbying Congress to end the 
exemption.

At issue in the study is a quirk of billing for lab procedures. Labs get paid 
based on the number of jars used to hold specimens from a prostate biopsy. 
Doctors can choose to put several specimens in one jar or put each in its own 
jar, potentially boosting lab fees, which averaged about $104 a jar in 2010, 
according to the study.

Urologists in practices with in-house pathologists sent 11.4 jars per biopsy 
for testing versus 5.9 jars per biopsy for other doctors in 2005.


Some doctors say that separating the samples can help them better map any 
cancer.

In addition, urologists in 

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[Histonet] RE: Flammable cabinets

[Podawiltz, Thomas]

Depends of the amount the cabinet is rated for. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Tuesday, April 10, 2012 4:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Flammable cabinets


Can anybody explain how much alcohol or other flammables we can store in a 
flammable cabinet in a room?  I have read the CAP guidelines and am still 
confused.  Do the CAP guidelines  only have to do with stored reagents outside 
of a flammable cabinet?
What am I missing?

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046



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