Re: [Histonet] Leica CV5030 coverslipper issues
If you are using slides with labels (esp. Ventana's), don't think the upgrade will fix it. We tear our hair out most days of the week with our 2 CV5030s. I wish Ventana would just make a coverslipper to use with their labels or change their labels to something thinner. Symphony takes too long to use as a coverslipper. histot...@imagesbyhopper.com histot...@imagesbyhopper.com 5/21/2012 3:30 PM We have the CV5030 and have had *many* issues. I have a graveyard of the composite racks with broken ears on them. The machine has broken racks and slides, thrown coverslips, dropped slides and we kept being told that we just needed adjustments. Later we were told that a new electronics board upgrade would fix the issue. My understanding is that the upgraded board is approximately $4000! I love Leica products, but this particular unit has not lived up to their reputation. These issues began almost immediately after purchase and continue 5 years later. We keep getting it repaired, but I have told the repair company, it was my position that Leica should have stepped up and replaced the board free of charge given all the issues we have endured! Michelle On May 21, 2012, at 3:00 PM, Horn, Hazel V hor...@archildrens.org wrote: We have a CV5030 coverslipper and we have had a few issues but Leica has been quick to resolve them. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hor...@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, May 21, 2012 1:15 PM To: 'Linda'; histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: RE: [Histonet] Leica CV5030 coverslipper issues We've had some issues that were mostly due to operator errors and lack of housekeeping, but they have been very good about correcting the problems. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Monday, May 21, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: [Histonet] Leica CV5030 coverslipper issues Hello Everyone, I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February. I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. I have emailed Leica several times now requesting a new coverslipper. If I have this many issues at three months what is it going to do in a year? The tech service support person has been great with fixing all of the issues. This is my reply I received from the President, North America, Jack Kenny- We do not believe that it is appropriate at this point to replace this system. We will continute to monitor the situation but not upgrade at this time. I am not looking for an upgrade I would just like a new coverslipper that works. Has anyone else had problems with Leica not replacing defective equipment? Please let me know. How did you resolve the issue? Thank you in advance, Linda Dee, BGS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original
Re: [Histonet] Leica CV5030 coverslipper issues
We do have the Ventana labeled slides, but coverslip them by hand. The issues we have are with standard, non-labeled slides In fairness to both Leica and the repair company we use, it has been a couple of months since we have had a real problem. Not sure if that is saying a lot, just wanted to be fair. Michelle On May 22, 2012, at 5:40 AM, Angela Bitting akbitt...@geisinger.edu wrote: If you are using slides with labels (esp. Ventana's), don't think the upgrade will fix it. We tear our hair out most days of the week with our 2 CV5030s. I wish Ventana would just make a coverslipper to use with their labels or change their labels to something thinner. Symphony takes too long to use as a coverslipper. histot...@imagesbyhopper.com histot...@imagesbyhopper.com 5/21/2012 3:30 PM We have the CV5030 and have had *many* issues. I have a graveyard of the composite racks with broken ears on them. The machine has broken racks and slides, thrown coverslips, dropped slides and we kept being told that we just needed adjustments. Later we were told that a new electronics board upgrade would fix the issue. My understanding is that the upgraded board is approximately $4000! I love Leica products, but this particular unit has not lived up to their reputation. These issues began almost immediately after purchase and continue 5 years later. We keep getting it repaired, but I have told the repair company, it was my position that Leica should have stepped up and replaced the board free of charge given all the issues we have endured! Michelle On May 21, 2012, at 3:00 PM, Horn, Hazel V hor...@archildrens.org wrote: We have a CV5030 coverslipper and we have had a few issues but Leica has been quick to resolve them. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hor...@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, May 21, 2012 1:15 PM To: 'Linda'; histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: RE: [Histonet] Leica CV5030 coverslipper issues We've had some issues that were mostly due to operator errors and lack of housekeeping, but they have been very good about correcting the problems. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Sent: Monday, May 21, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Cc: kristy.han...@leica-microsystems.com; jack.ke...@leica-microsystems.com; paul.raimo...@leica-microsystems.com Subject: [Histonet] Leica CV5030 coverslipper issues Hello Everyone, I purchase a brand new Leica CV5030 coverslipper, which I received at the beginning of February. I have had non- stop issues with this coverslipper from it throwing slides, coverslips and now the sensor not working properly. I have emailed Leica several times now requesting a new coverslipper. If I have this many issues at three months what is it going to do in a year? The tech service support person has been great with fixing all of the issues. This is my reply I received from the President, North America, Jack Kenny- We do not believe that it is appropriate at this point to replace this system. We will continute to monitor the situation but not upgrade at this time. I am not looking for an upgrade I would just like a new coverslipper that works. Has anyone else had problems with Leica not replacing defective equipment? Please let me know. How did you resolve the issue? Thank you in advance, Linda Dee, BGS, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: CAP vs. CLIA
Thank You Amanda!!! - Original Message - From: Amanda Kelley akell...@slu.edu To: WILLIAM DESALVO wdesalvo@hotmail.com Cc: histonet histonet@lists.utsouthwestern.edu Sent: Monday, May 21, 2012 9:53:41 AM Subject: Re: [Histonet] RE: CAP vs. CLIA I usually do not comment on the histonet, however this topic is near and dear to my heart. We in Histology are support scientific staff to the Pathologist as much as the Laboratory director is to the Pathologist. Scientific duties, which a pathologist can perform (histological and cytological preparation has been delegated down) to our position. Interpretation of Controls and associated material is often left to us and the Pathologist assistant We determine what the Pathologist can interpret Our expertise directly determines patient outcome, if we are not professional in our decisions then the Pathologist is ineffective in his or hers. The Professional business of running the laboratory is performed by the medical laboratory director, and associate director. These are professional positions within the laboratory where a Pathologist has delegated the managerial responsibility to a subordinate. According to CLIA, this position does not exist, only the Pathologist can truly be the Medical laboratory director. Yet in hospitals across the country many Clinical laboratory Scientist's who don't know anything or have limited knowledge of AP are in charge of our AP labs.Their positions are listed as Laboratory Director. This subordinate is the most important person in the lab, yet they too do not turn out results. They are frequently the highest paid in the lab. Their pay is based on the designation of their scientific and managerial background. I believe we can do the same. The business of the pathology lab would be very difficult for the pathologist to handle without this symbiotic relationship of the scientific businessman to the Pathologist. Likewise a Pathologist can not perform their job without the symbiotic relationship to the histotechnologist.Thereby technically, making our jobs indispensable to the Pathologist much like the laboratory director. Our problem is history, where many pathologists trained their out of work brother in law to do the work. Many rural areas still run their labs this way. As long as there is a path to become a histotech by hiring whomsoever to do the job. We will always be left behind. So I believe if CLIA and CAP can recognize the laboratory Manager as an important professional position delegated by the Pathologist then we should be afforded the same courtesy. On Sun, May 20, 2012 at 10:37 PM, WILLIAM DESALVO wdesalvo@hotmail.comwrote: I seemed to have missed something or it might have been all the fresh sea air I got in Tampa at the FSH, but I do not understand the outrage expressed towards CLIA and CAP because we are not listed as testing personnel. I applaud everyone's passion for Histotechnology and the outrage that we are not allowed to fully participate in the test system model, but I think we should be directing more of our outrage to the individuals working in Histotechnology that are not and will not take responsibility to increase the professionalism of our profession and our own acceptance of the current state of Histotechnology. A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results. As good as we are and as complex parts of the Histotechnology process may be, Histotechnicians, Histotechnologists and Pathology Assistants do not meet the standard stated and do not participate in the post-analytic phase, produce and release patient results. We simply are not able to be credentialed as is the Medical Technologists and Cytotechnologist. I am not saying any one laboratory professional group is better than the other, just that to be considered testing personnel, we must be properly credentialed. Collectively, we as a discipline, science and group should be working to upgrade our education requirements and training so that we can become fully invested partners with the Pathologist. We, not CAP or CLIA, must greatly increase our professionalism before we can truly be considered competent to work in the post-analytical phase. I cannot today accept that every working Histotechnician, Histotechnologist and Pathologist Assistant is able to produce the result and release. I am quite sure that every Medical Technologist and Cytotechnologist is capable and competent to produce and release a patient result. As things stand today, Histotechnology and all of us the working in this discipline are a support function to the one person in our discipline, the Pathologist, that is educated, trained, credentialed and competent to produce and release a patient result. I also believe there are many opportunities within
Re: [Histonet] Leica CV5030 coverslipper issues
We had a bunch of issues with our 5030 coverslipper that have largely been resolved with the following top tips!: a) Making sure that the coverslips we load into the machine are not statically-charged (they don't lie flat against each other - the pile feels springy). The brand we use only has occasional boxes of 100 coverslips like this, we just use them for hand-mounting instead. b) Scrupulous housekeeping (clean any DPX/glass splinters away regularly) Phil - Mr Philip Gibson Business Manager Directorate of Medicine Newcastle Upon Tyne Hospitals NHS Foundation Trust Tel. 0191 2821511 DECT 21511 This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. This email has been processed by SmoothZap - www.smoothwall.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NSH 5th Annual Summer Symposium
NSH is bringing the 5th Annual Summer Symposium back to Las Vegas, NV! General Sessions and workshops featuring expert speakers will provide you with the tools, advice, and guidance you seek in your professional career. The Summer Symposium is one of the best values for your training dollars in histology education, offering 12 continuing education credits and an exhibit fair for one low price! Current Exhibitors Include: Ameripath/Dermpath Diagnostics, Biocare Medical, Cell Marque Corporation, Leica Microsystems, MediaLab, Inc., Sakura Finetek USA, Simport Scientific, Statlab Medical Products, and Anatech Ltd. Registration Fees: Members: $229 Non-Members: $249 Student Members: $169 HT Readiness Course Only: $99 Registration Brochure: https://s3.goeshow.com/nsh/SS2012/PDF/SSBrochureforWeb.pdf Click here for more details and to register online! https://s3.goeshow.com/nsh/SS2012/ereg419568.cfm?pg=home Have a great week and we hope to see you in Vegas! Jessica Smith Meeting Coordinator/Social Media Specialist National Society for Histotechnology 10320 Little Patuxent Parkway #804 Columbia, MD 21044 Phone: 443-535-4062 Fax:443-535-4055 jess...@nsh.org | www.nsh.org www.histoconvention.org Follow us online for the latest news/updates! Facebookhttp://www.facebook.com/profile.php?id=12114656174 Twitterhttp://twitter.com/#!/NS4Histotech Linked Inhttp://www.linkedin.com/pub/jessica-smith/2a/201/284 YouTubehttp://www.youtube.com/user/TheHistoTube?ob=0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica CV5030 coverslipper issues
We also have the person taking care of the coverslipper change the brush daily and keep the one in use wet with xylene several times a day. This seems to expand the life of the dispensing needle and keep it from bending or breaking. We are doing everything else suggested and the problems are gone. We have a couple of repair issues however; that were small and quickly fixed. Pam - Original Message - From: Philip Gibson philip.gib...@nuth.nhs.uk To: histonet@lists.utsouthwestern.edu Sent: Tuesday, May 22, 2012 8:16:14 AM Subject: Re: [Histonet] Leica CV5030 coverslipper issues We had a bunch of issues with our 5030 coverslipper that have largely been resolved with the following top tips!: a) Making sure that the coverslips we load into the machine are not statically-charged (they don't lie flat against each other - the pile feels springy). The brand we use only has occasional boxes of 100 coverslips like this, we just use them for hand-mounting instead. b) Scrupulous housekeeping (clean any DPX/glass splinters away regularly) Phil - Mr Philip Gibson Business Manager Directorate of Medicine Newcastle Upon Tyne Hospitals NHS Foundation Trust Tel. 0191 2821511 DECT 21511 This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. This email has been processed by SmoothZap - www.smoothwall.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Monoclonal antibodies from different companies
Dear Histonetters, If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, is it the same? In other words, we are using clone #XX#X from Company A. Someone else has had success using clone #XX#X from Company B. Is there any benefit to buying the antibody from Company B to try, or would it be a duplication of effort and cost? Thanks for your input! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Monoclonal antibodies from different companies
In general, the antibody itself is the same antibody. Clone types are generally purchased by different companies from the same source. They are usually purchased by the supplying company highly concentrated and then prepared for secondary sale by diluting (even when purchasing concentrating antibodies) and adding buffers and proprietary stabilizers. Antibodies treated in this way can vary substantially from one company to another. Companies also buy cell lines that will retain the same clone number, but will be grown by the ihc supply company in house and prepared as above. These can be better or worse. In general, the best clones are proprietary clones that are sold by the individual company - usually discovered or purchased while partnering with a researcher or educational institution. These can be among the best quality products supplied by an ihc company because the company is putting its best foot forward and wants to protect their exclusive rights. I can provide specifics off-line. Who knows I may work for one of those companies in the future or may have worked for them in the past. Will Chappell, HTL Sent from my iPhone On May 22, 2012, at 11:08 AM, Teri Johnson tjohn...@gnf.org wrote: Dear Histonetters, If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, is it the same? In other words, we are using clone #XX#X from Company A. Someone else has had success using clone #XX#X from Company B. Is there any benefit to buying the antibody from Company B to try, or would it be a duplication of effort and cost? Thanks for your input! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HTL exam
Heather, I took the exam last year and used the ASCP guidelines along with the Carson/Hladik (3rd edition) text, the Carson/Hladik Histodeck flash cards, and NSH practice question booklets. I am not sure how old the NSH booklets are because another tech (thankfully!) let me borrow them, but they were extremely helpful. The HistoDeck cards were nice for studying, however the quality of HistoDeck pictures are *much* better than those of the exam. Here is a link to some study materials from NSH http://www.nsh.org/content/certification-exam-study-aids Goodluck!!! Amanda Phipps, HTL (ASCP)cm Histotechnologist Licking Memorial Hospital From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heather [hcaouett...@gmail.com] Sent: Monday, May 21, 2012 11:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL exam Hi, I am taking the HTL exam in about three months and would really appreciate any guidance that anyone has, study tips, etc. I have already downloaded the ASCP information on the test. Thanks! -Heather ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Monoclonal antibodies from different companies
In my experience, identical clones from different companies are not always equivalent. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Tuesday, May 22, 2012 9:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Monoclonal antibodies from different companies Dear Histonetters, If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, is it the same? In other words, we are using clone #XX#X from Company A. Someone else has had success using clone #XX#X from Company B. Is there any benefit to buying the antibody from Company B to try, or would it be a duplication of effort and cost? Thanks for your input! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Monoclonal antibodies from different companies
Teri, this is an excellent question. Short answer: the same antibody clone from different company's may produce different results. Usually it will be a dilution factor but could be specificity as well if not purified to the same level. There is no way to tell for sure without trying them both. The fact is that some companies make their antibodies and control the entire process. Others repackage antibodies they buy from other companies. Others sell a mix of their own and repackaged. Most likely they won't tell you which is which. Even the clone doesn't make much difference because the companies may each have licensed the same clone from the originator of that clone. Theoretically clones should be the same, but unless they come from the same facility they may not be. One company's production process will be slightly different than another's and so the antibody may produce slightly different results. This can be due to impurities, level of purification, dilution levels (yes they dilute the antibody, even in concentrate form!) buffers, etc. Tim Morken Department of Pathology UC San Francisco Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Tuesday, May 22, 2012 8:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Monoclonal antibodies from different companies Dear Histonetters, If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, is it the same? In other words, we are using clone #XX#X from Company A. Someone else has had success using clone #XX#X from Company B. Is there any benefit to buying the antibody from Company B to try, or would it be a duplication of effort and cost? Thanks for your input! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Monoclonal antibodies from different companies
Agree with Tresa. It could happen because some companies purchased original clones (not Ig concentrate for further distribution) and screw them. I had such problems occasionally, in particular with now extinct Chemicon. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: agleiber...@cbiolabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Tuesday, May 22, 2012 11:23 AM To: 'Teri Johnson'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Monoclonal antibodies from different companies In my experience, identical clones from different companies are not always equivalent. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Tuesday, May 22, 2012 9:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Monoclonal antibodies from different companies Dear Histonetters, If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, is it the same? In other words, we are using clone #XX#X from Company A. Someone else has had success using clone #XX#X from Company B. Is there any benefit to buying the antibody from Company B to try, or would it be a duplication of effort and cost? Thanks for your input! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] test
NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
I agree and would like to add. This is one scenario that I have seen many times, where hospitals or wherever will hire histotechs without certifications . I am thinking that saves them money and they still have a histotech. I have seen good histotechs that have no certification and likewise some bad ones with certification. Lately I have seen these schools turn out histotechs , it seems with little encouragement to get certified. If places will hire them without being certified, there seems little incentive (unless you are self motivated for more money) to move on up to certification. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
That seems to be the unfortunate situation at this time... Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Tue, 22 May 2012 12:39:39 -0500 From: sherrian.mc...@va.gov To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) I agree and would like to add. This is one scenario that I have seen many times, where hospitals or wherever will hire histotechs without certifications . I am thinking that saves them money and they still have a histotech. I have seen good histotechs that have no certification and likewise some bad ones with certification. Lately I have seen these schools turn out histotechs , it seems with little encouragement to get certified. If places will hire them without being certified, there seems little incentive (unless you are self motivated for more money) to move on up to certification. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] P 40 Antibody
Biocare has it. IVD... Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ADESUPO ADESUYI [adesupo2...@hotmail.com] Sent: Monday, May 21, 2012 11:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P 40 Antibody Hi, I am looking for informations on P 40 Antibody. I want informations like the vendor, procedure e.t.c. Thanks, Adesupo Adesuyi, HTL(ASCP)QIHC NRH, Norman, OK ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HTL exam
I did the BOR Study guide Second Edition and passed 4 years ago. Carl Ryan Nituda, HTL(ASCP) QIHC Pathology Sciences Medical Sent from my iPhone On May 22, 2012, at 8:21, Amanda Phipps aphi...@lmhealth.org wrote: Heather, I took the exam last year and used the ASCP guidelines along with the Carson/Hladik (3rd edition) text, the Carson/Hladik Histodeck flash cards, and NSH practice question booklets. I am not sure how old the NSH booklets are because another tech (thankfully!) let me borrow them, but they were extremely helpful. The HistoDeck cards were nice for studying, however the quality of HistoDeck pictures are *much* better than those of the exam. Here is a link to some study materials from NSH http://www.nsh.org/content/certification-exam-study-aids Goodluck!!! Amanda Phipps, HTL (ASCP)cm Histotechnologist Licking Memorial Hospital From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heather [hcaouett...@gmail.com] Sent: Monday, May 21, 2012 11:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL exam Hi, I am taking the HTL exam in about three months and would really appreciate any guidance that anyone has, study tips, etc. I have already downloaded the ASCP information on the test. Thanks! -Heather ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD133 on Ventana Benchmark Ultra
Hi histonetters! Can anyone provide me with a successful protocol for CD133/1 from Miltenyi on the Ventana Benchmark Ultra? Today I searched the web for this antibody. It seems like this company has the exclusive right for selling it.?. Their website contents also a forum. There are some posts of unsatisfied costumers with problems to get the IHC working on FFPE tissue. Any experiences? Thanks in advance Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reagent containers???
Does anyone have reagent containers and slide baskets for a Shandon LTD Varistain XY Slide stainer that they would be willing to get rid of? Feel free to reply to my personal email addy Thanks Heather ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Monoclonal antibodies from different companies
Hi Teri If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, IT IS THE SAME. There is no any benefit in buying the antibody from Company B to try, since it is a duplication of effort and cost Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Wednesday, 23 May 2012 1:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Monoclonal antibodies from different companies Dear Histonetters, If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, is it the same? In other words, we are using clone #XX#X from Company A. Someone else has had success using clone #XX#X from Company B. Is there any benefit to buying the antibody from Company B to try, or would it be a duplication of effort and cost? Thanks for your input! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cytology Staining
Is Cyto prep considered a high complexity task? On May 17, 2012, at 12:59 PM, Yang, Mari my...@emc.org wrote: Sheila, Maybe the inspector was referring to the prep? Many cytology labs use automated staining. If you find out any information, I'd love to know. Thanks, Mari Mari Yang, MHA, CT(ASCP)CMHTLCM Cytology Supervisor Tel: 760.773.2009 P Save a tree, please don't print this e-mail unless you really need to. Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Haas Sent: Thursday, May 17, 2012 4:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cytology Staining Hello all. During an inspection yesterday, the inspector made a brief, vague reference to cytology staining now being deemed high complexity. Does anyone have information and/or a reference for this? I'd appreciate any information anyone could provide. Thanks a bunch. Sheila Haas Laboratory Manager MicroPath Laboratories, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Monoclonal antibodies from different companies
Gee I do live in an ideal world, looking through rose coloured glasses - definitely affects my interpretation of an HE stain! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Wednesday, 23 May 2012 10:48 AM To: 'Teri Johnson'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Monoclonal antibodies from different companies Hi Teri If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, IT IS THE SAME. There is no any benefit in buying the antibody from Company B to try, since it is a duplication of effort and cost Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Wednesday, 23 May 2012 1:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Monoclonal antibodies from different companies Dear Histonetters, If you can get a monoclonal antibody from different sources, the same clone, the same Ig concentration, is it the same? In other words, we are using clone #XX#X from Company A. Someone else has had success using clone #XX#X from Company B. Is there any benefit to buying the antibody from Company B to try, or would it be a duplication of effort and cost? Thanks for your input! Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 102, Issue 26
I'm a Histotechnologist working in the Regional Hospital in Barrie, ON Canada. We are using the Ventana Ultra for our Immunohistochemistry (IHC). Since the end of February, we have been having issues with some tissues lifting off our positive (marked with +) charged slides. It seems to be mostly with the fatty and/or larger sections. We now dry our slides for one hour at room temperature (R.T.) and an additional hour at 60 degrees C. We cut our IHC sections at 4 um. Since we have tried 2 different types of + slides and will be trying another type of charged slide (from Newcomer this time) I was wondering if anyone has any other suggestions? I also have another question regarding a QC (quality control) issue. We use a multi-tissue control that is applied to the top of all our test slides for IHC. One of our paths commented that there is some positive staining in the smooth muscle nuclei of thenormal bowel when we are testing for Progesterone (PR). We are using a Heat Induce Epitope Retrieval (HEIR) of 36 minutes with CC1 (Ventana's proprietary buffer @ pH of 8.0-8.5) and a primary antibody incubation time of 16 minutes with PR clone 1E2. (Ventana instrumentation provides pre-diluted antibodies and the user adjusts the concentration of the antibody by adjusting the time the primary antibody is incubated with the tissue). I am concerned about the implications of this staining and I have not been able to find a reference to this kind of unusual staining pattern. The bowel tissue that we are using as QC is from a 62 year old female patient. I was wondering if anyone has had any experience with this kind of staining and /or any references that I could use. Thanking you in advance, I look forward to your input, Nancy Cloughley-Gray MLT Nancy, Your problem is very common, due to fatty tissue is difficult to process. Here's my suggestion if you want to salvage your fatty tissue blocks (presumably the fatty tissues are well fixed); 1) melt down the fatty tissue block re-process 2) cut put on charged slides (Fisher Scientific Ultra Stick charged slides work the best) 3) bake slide for 30 min @ 60c 4) do antigen retrieval with Biocare's Diva for 3 min in Pascal or Biocare pressure cooker (very important; do NOT use Triology Cell Marque pressure cooker). 5) do your ihc as usual Three important elements; (1) well process tissue, (2) Biocare Diva AR buffer, (3) Pascal pressure cooker. Keep us posted what's your outcome. Madeleine Huey BS, HTL/QIHC (ASCP) Supervisor - Pathology (histology IPOX) madelein...@elcaminohospital.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
