RE: [Histonet] Fluorescence-filters
Sure you can, all you need is a bright enough lightsource which emits at the correct wavelength. If the spectrum of the light source is too wide, you dump in a filter to narrow it down to your desired excitation. Flow cytometers nowadays even use LED lasers instead of the huge, bulky and vulnerable gas lasers of the old days. Which is a good thing. Jonathan --- Jonathan Cremer Laboratory Technician TARGID - KU Leuven Van: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] namens Emily Sours [talulahg...@gmail.com] Verzonden: woensdag 6 juni 2012 1:31 Aan: histonet@lists.utsouthwestern.edu Onderwerp: Re: [Histonet] Fluorescence-filters Can you do fluorescence with LED? (That may be a stupid question, as I always thought LED was just for brightfield). Emily You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir. --Howard Moon, in Charlie, The Mighty Boosh ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Fluorescence-filters
We recently installed a LED light source on our fluorescence scope that our pathologists use for reading IF on kidneys and skins. They love it. No more having to warm up the bulb, not turning it off too soon, or my nightmare - leaving it on overnight. The bulb is supposed to last a really long time and gives off a nice bright light. Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, June 05, 2012 7:32 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fluorescence-filters Can you do fluorescence with LED? (That may be a stupid question, as I always thought LED was just for brightfield). Emily You see a peanut, day's off to a good start; you witness some soil it's a jamboree for Vince Noir. --Howard Moon, in Charlie, The Mighty Boosh ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet To report this email as SPAM, please forward it to s...@websense.com. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech scheduling
I am looking for some creative scheduling ideas. We have a day shift crew that starts at 4am and goes til 530pm. The schedule is below. Techs would like to rotate to do different jobs within the department but don't really want to rotate hours. Can I please get some examples of how others of you are staffing? Even if you have a second or third shift I would like to see some different workflow plans and how they are staffed. 1 HT 400am - Embedder 2 HTs 500am - Cutters 1 HT 730 - Immunos/specials/orders 1 HT 830 - Grossing/pathologist assist 1 lab assistant 800am - cytology prep/gross room assist Aimee M. Duddey, MLT(ASCP) Moore Regional Hospital Laboratory Assistant Director of Laboratory - Pathology 910-715-5286 FirstHealth OF THE CAROLINAS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histotech scheduling
http://www.biomedcentral.com/1472-6890/10/2 Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Wed, 6 Jun 2012 08:30:47 -0400 From: adud...@firsthealth.org To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotech scheduling I am looking for some creative scheduling ideas. We have a day shift crew that starts at 4am and goes til 530pm. The schedule is below. Techs would like to rotate to do different jobs within the department but don't really want to rotate hours. Can I please get some examples of how others of you are staffing? Even if you have a second or third shift I would like to see some different workflow plans and how they are staffed. 1 HT 400am - Embedder 2 HTs 500am - Cutters 1 HT 730 - Immunos/specials/orders 1 HT 830 - Grossing/pathologist assist 1 lab assistant 800am - cytology prep/gross room assist Aimee M. Duddey, MLT(ASCP) Moore Regional Hospital Laboratory Assistant Director of Laboratory - Pathology 910-715-5286 FirstHealth OF THE CAROLINAS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histotech scheduling
Please unsubscribe me. thanks -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: 06 June 2012 15:14 To: adud...@firsthealth.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histotech scheduling http://www.biomedcentral.com/1472-6890/10/2 Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Wed, 6 Jun 2012 08:30:47 -0400 From: adud...@firsthealth.org To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histotech scheduling I am looking for some creative scheduling ideas. We have a day shift crew that starts at 4am and goes til 530pm. The schedule is below. Techs would like to rotate to do different jobs within the department but don't really want to rotate hours. Can I please get some examples of how others of you are staffing? Even if you have a second or third shift I would like to see some different workflow plans and how they are staffed. 1 HT 400am - Embedder 2 HTs 500am - Cutters 1 HT 730 - Immunos/specials/orders 1 HT 830 - Grossing/pathologist assist 1 lab assistant 800am - cytology prep/gross room assist Aimee M. Duddey, MLT(ASCP) Moore Regional Hospital Laboratory Assistant Director of Laboratory - Pathology 910-715-5286 FirstHealth OF THE CAROLINAS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Department of Agriculture, Food and the Marine The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). An Roinn Talmhaíochta, Bia agus Mara Tá an t-eolais san ríomhphost seo, agus in aon ceangláin leis, faoi phribhléid agus faoi rún agus le h-aghaigh an seolaí amháin. D’fhéadfadh ábhar an seoladh seo bheith faoi phribhléid profisiúnta nó dlíthiúil. Mura tusa an seolaí a bhí beartaithe leis an ríomhphost seo a fháil, tá cosc air, nó aon chuid de, a úsáid, a chóipeál, nó a scaoileadh. Má tháinig sé chugat de bharr dearmad, téigh i dteagmháil leis an seoltóir agus scrios an t-ábhar ó do ríomhaire le do thoil. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] staffing questions
I have been asked to do some research and provide some facts and figures as it relates to staffing of histology labs. If anybody is willing to answer the questions listed below, I would appreciate your input. You may email me at privately and all information will be kept confidental. 1. Staffing levels, i.e. # of pathologists, techs, PAs, and Lab aides 2. Approximate workload, either blocks per day or cases per year 3. Salary for HTs and supervisors (I know this can be a sticky area but any info would be helpful) Thank You in advance Allison Hutton, HTL(ASCP)cm Lead Tech Histology Doylestown Hospital ahut...@dh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] staffing questions
Staff level (your question 1) depends on the answer to your question 2 and also depends on the productivity level, both individual and collective. The salaries depend on the nature of your lab (non for profit, private for profit or governmental) and with the total benefits offered. But also depend on the market availability and the willingness to negotiate both from the administration and the potential or existing employees. So you ca understand both issues I am sending you some information under separate cover. René J. From: Hutton, Allison ahut...@dh.org To: histonet@lists.utsouthwestern.edu Sent: Wednesday, June 6, 2012 11:09 AM Subject: [Histonet] staffing questions I have been asked to do some research and provide some facts and figures as it relates to staffing of histology labs. If anybody is willing to answer the questions listed below, I would appreciate your input. You may email me at privately and all information will be kept confidental. 1. Staffing levels, i.e. # of pathologists, techs, PAs, and Lab aides 2. Approximate workload, either blocks per day or cases per year 3. Salary for HTs and supervisors (I know this can be a sticky area but any info would be helpful) Thank You in advance Allison Hutton, HTL(ASCP)cm Lead Tech Histology Doylestown Hospital ahut...@dh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sequenza units for manual immunohistochemistry
Hello Histonet, I'm just wondering if anyone out there is using these units for their manual staining, and if anyone has ever given any consideration to what happens when you have a large volume of reagent waste in the bottom from all your additions and washes. Since the units draw reagents through with capillary action, what do you think happens when the bottom of the slide/coverplate combo is immersed in the cocktail that is created in the bottom. Has anyone had any issues using these units? How do people prevent a large volume from building up in the bottom? How are people cleaning their coverplates and the units themselves? Thanks in advance for your thoughts! Keri Keri Colwell, BSc Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640 | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colw...@inspection.gc.ca Telephone | Téléphone: 403-382-5500 ext 5613/5511 Facsimile | Télécopieur: 403-382-5585 Government of Canada | Gouvernement du Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sequenza units for manual immunohistochemistry
I have used them for quite some time. I was having problems with uneven staining and determined that it was from the being too full and was drawing up liquid from the bottom. There is a small opening on the end and I just empty the solution from it. I usually empty it after rinsing the antibody off and then usually before putting DAB or DAPI on depending on the number of slides stained. As for washing, I just wash in a solution of soapy water and then rinse in distilled. I use the coverplates for several staining times. I love the sequenza and prefer it to having an automatic stainer. Sharon Beckham, HT (ASCP) Head, Histotechnology Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, Missouri 64110 (816)926-4305 s...@stowers.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Keri Colwell Sent: Wednesday, June 06, 2012 12:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sequenza units for manual immunohistochemistry Hello Histonet, I'm just wondering if anyone out there is using these units for their manual staining, and if anyone has ever given any consideration to what happens when you have a large volume of reagent waste in the bottom from all your additions and washes. Since the units draw reagents through with capillary action, what do you think happens when the bottom of the slide/coverplate combo is immersed in the cocktail that is created in the bottom. Has anyone had any issues using these units? How do people prevent a large volume from building up in the bottom? How are people cleaning their coverplates and the units themselves? Thanks in advance for your thoughts! Keri Keri Colwell, BSc Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640 | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colw...@inspection.gc.ca Telephone | Téléphone: 403-382-5500 ext 5613/5511 Facsimile | Télécopieur: 403-382-5585 Government of Canada | Gouvernement du Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] processor solution rotation
May I suggest? Define a standard that works for you, combining both elements, blocks and runs e.g., Every week or 200 blocks then check the first reagent volume and quality. Again at 400 And again at 600 blocks. We change the first reagent whenever it appears dirty. Consider adding a few ccs. of eosin as a visual indicator in the first 100% alcohol to monitor carryover and number of runs. When the indicator reaches the first xylene, time to rotate or change. NOTE Eosin fluoresces. The actual blocks and runs number varies on specimen size and fixation, (unfixed tissues use up the alcohols) and also whether you use sponges (more carryover) and whether the processor drains/pumps out completely-carryover. And on how fast the processing cycle is- short cycles demand fresh reagents and leave little room for error. When in doubt do not hesitate to replenish the last solution of each type, alcohol, xylene and paraffin or change all solutions. Steve Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pathologists' Assistant Job in Mid-West
Hi Everyone, I have a Pathologists' Assistant Job in the MidWest that is a full time permanent job. I am looking for an ASCP Certified Pathologists' Assistant, with a Master's Degree from an NAACLS accredited institution. Please email me for more details. Thank you@ To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php Melissa Phelan LinkedIn: http://www.linkedin.com/in/melissaphelan President, Laboratory Staffing Allied Search Partners P: 888.388.7571 F: 888.388.7572 M: 407.697.1175 www.alliedsearchpartners.com http://www.alliedsearchpartners.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Approval and review of procedures
Hello, I am currently helping organize histology SOPs and need some help clarifying who can perform SOP review. In cytology, the technical supervisor (MD) reviews and approves procedures. CLIA rules for clinical departments allow MT as technical supervisors. Can an HT be a technical supervisor and perform annual review of SOPs? Judy Sproles CT(ASCP Methodist. Leading Medicine. Recognized by U.S.News World Report as one of America's Best Hospitals in 13 specialties. Named to FORTUNE® Magazine's 100 Best Companies to Work For® list seven years in a row. Designated as a Magnet hospital for excellence in nursing. Visit us at methodisthealth.com. Follow us at twitter.com/MethodistHosp and www.facebook.com/MethodistHospital. ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain restricted and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Approval and review of procedures
No, it has to be the pathologist who is designated as the technical supervisor for the lab. Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.mor...@ucsfmedctr.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sproles, Judy A. Sent: Wednesday, June 06, 2012 2:30 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Approval and review of procedures Hello, I am currently helping organize histology SOPs and need some help clarifying who can perform SOP review. In cytology, the technical supervisor (MD) reviews and approves procedures. CLIA rules for clinical departments allow MT as technical supervisors. Can an HT be a technical supervisor and perform annual review of SOPs? Judy Sproles CT(ASCP Methodist. Leading Medicine. Recognized by U.S.News World Report as one of America's Best Hospitals in 13 specialties. Named to FORTUNE® Magazine's 100 Best Companies to Work For® list seven years in a row. Designated as a Magnet hospital for excellence in nursing. Visit us at methodisthealth.com. Follow us at twitter.com/MethodistHosp and www.facebook.com/MethodistHospital. ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain restricted and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Approval and review of procedures
And not her/his designee? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, June 06, 2012 5:40 PM To: Sproles, Judy A.; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Approval and review of procedures No, it has to be the pathologist who is designated as the technical supervisor for the lab. Tim Morken Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94132 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.mor...@ucsfmedctr.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sproles, Judy A. Sent: Wednesday, June 06, 2012 2:30 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Approval and review of procedures Hello, I am currently helping organize histology SOPs and need some help clarifying who can perform SOP review. In cytology, the technical supervisor (MD) reviews and approves procedures. CLIA rules for clinical departments allow MT as technical supervisors. Can an HT be a technical supervisor and perform annual review of SOPs? Judy Sproles CT(ASCP Methodist. Leading Medicine. Recognized by U.S.News World Report as one of America's Best Hospitals in 13 specialties. Named to FORTUNE® Magazine's 100 Best Companies to Work For® list seven years in a row. Designated as a Magnet hospital for excellence in nursing. Visit us at methodisthealth.com. Follow us at twitter.com/MethodistHosp and www.facebook.com/MethodistHospital. ***CONFIDENTIALITY NOTICE*** This e-mail is the property of The Methodist Hospital and/or its relevant affiliates and may contain restricted and privileged material for the sole use of the intended recipient(s). Any review, use, distribution or disclosure by others is strictly prohibited. If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender and delete all copies of the message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Reagent rotation
Dear Mark We change the solution depend upon no of cassettes run. In your case the size of specimen is small so u can change/ rotate depend upon no of run.i felt that the size of the specimen make big impact on reagents in Tissue Processer. With regards Dr G vijay Orchid Pharma -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, June 06, 2012 10:30 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 103, Issue 7 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Reagent rotation (Mark Perrin) 2. RE: Reagent rotation (Goins, Tresa) 3. Reagent rotation (Stephenson, Sheryl) 4. Re: Fluorescence-filters (Eric Hoy) 5. Current Histology Openings (Brannon Owens) 6. Re: Fluorescence-filters (Mark Tarango) 7. NEW Position Alert - Quality Assurance Histologist (Matt Ward) 8. Re: Fluorescence-filters (Emily Sours) 9. Re: Fluorescence-filters (Emily Sours) 10. RE: Reagent rotation (Ian R Bernard) 11. RE: Fluorescence-filters (Jonathan Cremer) 12. RE: Fluorescence-filters (Sue Hunter) 13. Histotech scheduling (Duddey, Aimee) 14. RE: Histotech scheduling (joelle weaver) 15. RE: Histotech scheduling (Fahy, Claire) 16. staffing questions (Hutton, Allison) 17. Re: staffing questions (Rene J Buesa) -- Message: 1 Date: Tue, 5 Jun 2012 11:04:50 -0700 (PDT) From: Mark Perrin perrintos...@yahoo.com Subject: [Histonet] Reagent rotation To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 1338919490.15637.yahoomail...@web44714.mail.sp1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 What is the normal procedure for changing the reagents in the tissue processor? We have a Sakura VIP6. Should the schedule of changing be based on number of runs vs. number of blocks processed? About 80% of our blocks are small GI biospies. Thanks in advance for your input. ? Toshia Perrin Medical Practice Coordinator Southern Pathology This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. -- Message: 2 Date: Tue, 5 Jun 2012 18:35:14 + From: Goins, Tresa tgo...@mt.gov Subject: RE: [Histonet] Reagent rotation To: 'Mark Perrin' perrintos...@yahoo.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: ca4df32ed505d94bb55e95487d8e98411f24c...@doaisd5205.state.mt.ads Content-Type: text/plain; charset=iso-8859-1 We rotate by cassette number - our daily load can vary from 10 to more than 100 cassettes, so an exchange schedule based on number of runs was not appropriate. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Perrin Sent: Tuesday, June 05, 2012 12:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reagent rotation What is the normal procedure for changing the reagents in the tissue processor? We have a Sakura VIP6. Should the schedule of changing be based on number of runs vs. number of blocks processed? About 80% of our blocks are small GI biospies. Thanks in advance for your input. ? Toshia Perrin Medical Practice Coordinator Southern Pathology This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 3 Date: Tue, 5 Jun 2012 14:02:36 -0500 From: Stephenson, Sheryl sstephen...@lifecell.com Subject: [Histonet] Reagent rotation To: 'Mark Perrin' perrintos...@yahoo.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: c2a8fe4da0ed8c468a3713f8607829d30d3f922...@amwpvex03.kci.com Content-Type: text/plain; charset=iso-8859-1 We do it by the number of Runs. About every 6 run for
