RE: [Histonet] cytology control slides?
???buccal.. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: 11 June 2012 15:48 To: pathr...@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cytology control slides? We make a buckle smear and stain it. Quick and easy. The QC is for the pap stain quality. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pathr...@comcast.net Sent: Monday, June 11, 2012 8:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cytology control slides? Fellow techs, I was wondering what some of you do for a control slide for your cytology stainer. We will be staining urine cytology specifically. Thanks, Ron Martin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: processors
VIP all the way. Still got one that I bought in 1985 as a backup Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, June 12, 2012 11:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processors Hi Histonetters- Is anybody using the ASP6025 from Leica? Pros and cons please. Then I would also appreciate any input on the Leica ASP300 vs Sakura VIP6. As always, Thank You for your help - it is highly appreciated. Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories 205 Bluff Street Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] OSU Job Posting
The Wexner Medical Center at The Ohio State University is looking for two (2) full time Histology Technicians/Technologists. The Ohio State is located in the heart of Columbus, Ohio. We have four beautiful seasons a year and enjoy a reasonable cost of living. Interested candidates should contact Lindsay Thorn at lindsay.th...@osumc.edu or 614-293-5048 The histology laboratories are functional area labs within the division of Surgical Pathology; processes, embeds, sorts, cuts, files, and stains slides of tissue samples for diagnosis by surgical pathologists at The Ohio State University Medical Center; logs in specimens into the computer system, performs special stains, prepares solutions, and maintains general organizational paperwork; assists with training of students and staff; monitors equipment maintenance; responsible for record keeping of specified quality control and quality assurance. Education and Experience: Graduate of a school of Histology with an HT (ASCP) certification or equivalent preferred; HTL certification desired; Bachelors degree in biological sciences preferred; 2 or more years previous experience in a histology laboratory preferred; courses in college level biological sciences are not required, but desirable; experience in other areas of pathology a plus. Requires successful completion of a background check. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HTL needed - FT Days--flexible hours
Small community hospital with a nice family feel (great schools/parks/cost of living) is seeking a registered HTL with a BS in one of the sciences. The conversation is simple and you can talk with the facility folks without any committment...we're looking for the perfect fit for the hospital AND for you. My most favorite jobs have been small community hospitals...knowing you've made a difference for your pathologist and your patient doesn't get any better than in small town hospitals. Give me a call--if this doesn't fit we'll work to find you a job that does! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone Fax ad...@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to apn...@fullstaff.org. Please include your name and specialty in the body of the email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Temp travelers needed
Hi Guys-- We're getting busy again and need a few more travelers. ---If you're curious, we'll spend some time answering your questions. ---If you're ready, we have a couple of short openings to talk about. Either way the conversation is fun and informative--the good, the bad, the straight scoop--histotech to histotech! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one GREAT Tech at a time. 281.852.9457 Office 800.756.3309 Phone Fax ad...@fullstaff.org Sign up for the FREE newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to apn...@fullstaff.org. Please include your name and specialty in the body of the email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Processing issue
That will depend on the tissue and the size/thickness of the specimens. Appreciate how they turned out and you will have the answer to your question. At least for me it is very difficult to answer your question without knowing the tissue characteristics. René J. From: Danny Zapata danny...@yahoo.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, June 13, 2012 12:57 AM Subject: [Histonet] Processing issue Would It be a grave problem if I run the tissue in a short run although it needed it to be placed in a routine regular run? Danny Zapata Dr. Norman Dermatology Office Tampa, Fl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Antigen retrieval
Does anyone have a favorite antigen retrieval method for FFPE mouse tissues that they would be willing to share? I have been using citrate buffer Ph6.0 with poor to moderate results. Thanks for any help! Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Antigen retrieval
Mike Retrieval methods are also based upon the antibody. During protocol development we try several different retrieval methods - no retrieval, enzymatic (proteinase K), pH6 and pH9, we then determine the best method and go from there. One retrieval method may not work for all antibodies. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Wednesday, June 13, 2012 8:27 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Antigen retrieval Does anyone have a favorite antigen retrieval method for FFPE mouse tissues that they would be willing to share? I have been using citrate buffer Ph6.0 with poor to moderate results. Thanks for any help! Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Antigen retrieval
Mike, A good retrieval method will restore the epitope to a configuration that the antibody will recognize. The data sheet received with a commercial antibody may suggest a retrieval method to use, but if you are using a non-commercial source, trying a variety of methods may help unless the epitope is irreversibly altered by fixation. We run all new assays using pH 9, pH 6 and sometimes pH 3 [10 min treatment rather than 20 min] in addition to enzymatic retrieval using Pepsin, Trypsin or Protease and finally No Retrieval. Happy Hunting, Tresa ___ Does anyone have a favorite antigen retrieval method for FFPE mouse tissues that they would be willing to share? I have been using citrate buffer Ph6.0 with poor to moderate results. Thanks for any help! Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cytology control slides?
I don't think there is a requirement for control slides for Cytology, only when IHCs and special stains are required. CAP requires a mechanism for monitoring cross-contamination. For urine cytology, I would insert a blank slide with your staining rack, if you are batch staining to ensure there are no floaters or cross contamination. Have the cytotech review the blank slide. The cytotech should also be monitoring the stain quality daily. Hope this helps. Thanks, Mari Mari Yang, MHA, CT(ASCP)CMHTLCM Cytology Supervisor Tel: 760.773.2009 P Save a tree, please don't print this e-mail unless you really need to. Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E. Sent: Wednesday, June 13, 2012 1:54 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cytology control slides? ???buccal.. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: 11 June 2012 15:48 To: pathr...@comcast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] cytology control slides? We make a buckle smear and stain it. Quick and easy. The QC is for the pap stain quality. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pathr...@comcast.net Sent: Monday, June 11, 2012 8:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cytology control slides? Fellow techs, I was wondering what some of you do for a control slide for your cytology stainer. We will be staining urine cytology specifically. Thanks, Ron Martin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] job opening
We currently have an open position for a HT, full time days. We are a community hospital located in southeastern PA. We average 11,000 cases per year, about half of which are GI biopsies. For more information or if interested, please visit www.dh.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Antigen retrieval
The most important part= about working up a new antibody is to have known positive tissue to use as= a control, if you do not have that everything you do is a shot in the dark= , you will not know if your protocol is not working or the control you are = using is not expressing that antigen. Each antibody has it's own requ= irement for pretreatment or not. We do what Liz does and sometimes ad= d in a ph8 buffer when nothing else works. Just to save on slides to = run we start with no ar and ph6 and if we do not get good results, go to ed ta ph9, then PK, then ph8, if all these fail, we revert to overnight incuba= tion of the primary ab after the most agressive high ph buffers and/or enzy= me digestion. If that fails we give up. This is all of course a= fter the best antibody titer has been determined. Some of my experien= ce points to antibodies that are expressed in the nuclei may prefer high ph= AR, this is just a trend I have noticed, definitely not something I h= ave proven. Regards,= /font Patsy Original Message Subject: [Histonet] RE: Antigen retrieval From: Elizabeth Chlipal= a [1]l...@premierlab.com = Date: Wed, June 13, 2012 7:32 am To: 'Mike Tighe' [2]mti...@trudeauinstitute.org, = [3]histo...@lists.uts= outhwestern.edu ([4]h isto...@lists.utsouthwestern.edu) [5]histonet@lists.utsouthwestern.edu Mike Retrieval methods are also based upon the antibody= . During protocol development we try several different retrieval methods -= no retrieval, enzymatic (proteinase K), pH6 and pH9, we then determine the= best method and go from there. One retrieval method may not work for all = antibodies. Liz Elizabeth A. Chlipala, BS, HTL(AS= CP)QIHC Manager Premier Laboratory, LLC PO Box 18592 = Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 = fax (303) 881-0763 cell [6]w= ww.premierlab.com Ship to address: 1567 Skywa= y Drive, Unit E Longmont, CO 80504 -Original Message= - From: [7]histonet-boun...@lists.utsouthwestern.edu [[8]mailto:histonet-bounces@lists.utsouthw= estern.edu] On Behalf Of Mike Tighe Sent: Wednesday, June 13, 201= 2 8:27 AM To: [9]hi= sto...@lists.utsouthwestern.edu ([10]histonet@lists.utsouthwestern.edu) Subject: [Histo= net] Antigen retrieval Does anyone have a favorite antigen ret= rieval method for FFPE mouse tissues that they would be willing to share? I= have been using citrate buffer Ph6.0 with poor to moderate results. Thanks= for any help! Mike __= _ Histonet mailing list [11]Histonet@lists.utsouthwestern.edu [12]http://lists.utsouthwestern.edu/mailman/listinfo/histonet = ___ Histonet mailing l= ist [13]Histonet@lis= ts.utsouthwestern.edu [14]http://lists.utsouthwestern.edu/mailman/listinfo= /histonet References 1. 3Dmailto:l...@premierlab.com; 2. file://localhost/tmp/3Dm 3. 3Dmailto:histonet@lists.utsouthwestern.edu; 4. 3Dmailto:histonet@lists.utsouthwestern.edu; 5. 3Dmailto:histonet 6. 3Dhttp://www.premierlab.com/ 7. 3Dmailto:histonet-bounces@lists.utsouthwestern.e 8. 3Dmailto:histon 9. 3Dmailto:histonet@lists.utsouthwestern.edu; 10. 3Dmailto:histonet@lists.utsou 11. file://localhost/tmp/3Dmail 12. 3Dhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet 13. 3Dmailto:Histonet@lists.utsouthwestern.edu; 14. 3Dhttp://lists.utsouthwestern.edu/___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Sequenza units for manual immunohistochemistry (Keri Colwell)
Hello, This is Tyler Liebig with the Thermo Scientific IHC group. I'm responding to Keri's questions regarding the Sequenza manual staining. Keri you are correct that if the waste is not emptied and builds up too much in the bottom of the sequenza unit it will definitely stop the capillary action (Reagent can't flow both ways :-). However, this can be avoided by emptying the waste regularly between runs. You bring up a good question though that I didn't know the answer to: How many slides can be stained before the waste hits the slides? I did a quick calculation below that I hope is helpful. Total Waste Volume of Sequenza Rack: In a Sequenza rack the slides are held about 1.5 inches above the bottom of the tray. This allows about 270ml of waste before the level is close touching the slides. Reagent use per slide: If you stain a single slide with a long protocol then worst case scenario is about 7 steps. (H2O2 through Counterstain with a two step polymer) Volume of rinse buffer: This means you would rinse 8 times 2ml each (16ml total rinse buffer per slide worst case). For the staining reagents: The recommended volume is 0.1ml per reagent and 0.5ml for chromogen. So for 6 reagents plus chromogen that is 1.1ml and I will double it because I know some people add way more than needed. So 2.2ml per slide worst case. So to sum it up worst case scenario 18.2ml of waste is created per slide which means 14 slides (270/ml/18.2ml=14.8) can be stained before the waste risks touching the slides. The rack holds ten slides total. In the end I recommend users play is save and empty the waste after each batch of slides stained even though most user generate much less waste than the calculations above. I also included a link to a great description of Sequenza use on the Histonet posted by Gayle M Callis HTL/HT/MT (ASCP). I have referenced these details frequently myself (Thanks Gayle). http://www.histosearch.com/histonet/Jul02A/Longansweronusetechnicswi.html Hope that helps and if you have any other questions I'm happy to help offline (contact information is below). Thank you, Tyler Liebig Americas Product Manager - Immunohistochemistry Thermo Scientific Anatomical Pathology (510) 979-5000 ex 31816 (510) 299-1751 mobile tyler.lie...@thermofisher.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Hot Hot Hot Histology Job Alert!! Please take a look at some exciting opportunities.
Hello Histonetters! I hope everyone is having a great day and keeping an eye out for armadillos!! :-) I have some new positions that I am excited to share. I am excited about these opportunities because some are with brand new labs, some are exclusive to RELIA and some are just really really good positions. Here is the list: HT/HTL - Philadelphia, PA Mohs Tech - Dallas Mohs Tech - West Virginia Lead histotech - Columbus, OH IHC Specialist - Long Island Electron Microscopy Specialist - Long Island Night Shift Histotech - Lafayette, LA Molecular Technologist - Nashville, TN Histology Supervisor - Aurora, IL Histology Supervisor - Boston, MA If you or anyone you know might be interested in information on any of these positions please contact me. I can be reached at rel...@earthlink.net or toll free at 866-607-3542. You could have an exciting new position sooner than you think OR a nice referral fee for spending cash for your summer vacation! Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com http://www.facebook.com/PamBarkerRELIA /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Varistain 24-4 Stainer Manual
I have an operator guide. Lynn Burton Lab Assoc I Animal Disease Lab Galesburg, Il 309-344-2451 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D [allison_sc...@hchd.tmc.edu] Sent: Tuesday, June 12, 2012 4:18 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Varistain 24-4 Stainer Manual Hello to all in histoland. Does anyone have a maual for the Varistain 24-4 stainer? I have a friend looking for one. Any help in this will be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TEM used needed
Hi I am looking to start a TEM lab in India. I heard that used TEMs are available if one is willing to pay for shipment. Can someone give some information? Thanks Abdullah ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Opportunity Northeast (NJ/PA/NY/MA)
Good Afternoon Everyone! We are searching for a histologist who is looking for an exciting career with a world leader in manufacturing histology products. Our client is looking for a Applications Support Specialist to be the Histology expert in the Northeast to provide customer and sales support, and troubleshooting. This position offers competitive base salary + bonus and full benefits. The best part about this job is it allows someone the opportunity to join a growing company that offers endless career growth. If this sounds like something you would be interested in please send me your resume and we can set a time to talk in more detail. Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
