[Histonet] RELIA HOT Histology Job Alert 6-19-2012 Exciting job opportunities with top employers ready to move now!!
Hi Histonetters! I hope everybody is having a great day. I have a few new jobs to share with you as the phone is ringing off the hook and the list of opportunities keeps growing and growing. All of these jobs are permanent full time positions with excellent pay and great benefits. The clients I am working with are growing labs and the rest of the crew at each of these labs can't wait to meet their new co-worker. If you are happy where you are please take a look anyway remember if I place someone you refer you will earn a referral fee. Can't we all use some extra fun money this summer? Here are the newest positions: HT/HTL - Portland, ME Mohs Tech - Irving, TX HT/HTL - Philadelphia Lead Histologist - Columbus, OH IHC Specialist - Long Island, NY (NYS license req.) I also have opportunities in IL, MA, KY and TN. For more information please contact Pam Barker at rel...@earthlink.net or toll free at 866-607-3542. *You have the histology expertise, use my recruiting expertise to find the right position for you! Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com http://www.facebook.com/PamBarkerRELIA /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE Stain for Epon-Embedded Tissue
Is it possible to perform an HE stain on tissues embedded in Epon plastic and cut into 0.5 - 1.0 micron sections? I can find protocols for doing so with tissues embedded in methacrylate, but not in Epon. Would the protocol for methacrylate also work for Epon, and if not, why? I am a student working on a research project in an electron microscopy lab and am trying to find a way to HE-stain thick sections, or find a stain that very closely approximates HE. Thank you in advance for any help you can offer! Shanon Pink University of Tennessee Health Science Center Department of Clinical Laboratory Science Memphis, TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: rat muscle fixation
Hello Sarah and Histonet You get wonderful formalin fixation of all rat tissues by transcardial perfusion - and no blood cells. You don't say whether you need a specific muscle and if it needs to be from a specific rat. Before you get into the bother of perfusing ( IACUC approval) you might want to contact your local neurology or neurobiology researchers because this is the method of choice for the brain. They will have lots of muscle as a by-product - the induced rigidity of the forelimb muscles (impressive to see the zombie effect) is a standard marker of good perfusion. You could check out some of their muscle to see if it is of a suitable quality. I'd ask the veterinarians in your animal facility to suggest someone who is doing this regularly, since this does require institutional approval as a non-survival surgery under deep anesthesia. -David == David A. Wright, Ph.D. University of Chicago Section of Neurosurgery, MC3026 Original message Histonet Digest, Vol 103, Issue 22 * Message: 2 Date: Mon, 18 Jun 2012 14:47:46 -0400 From: Pixley, Sarah (pixleysk) pixle...@ucmail.uc.edu Subject: [Histonet] RE: Histonet Digest, Vol 103, Issue 22 To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: e13205c9cb112a44b029c1c3f20eb8ff86482a5...@ucmailbe5.ad.uc.edu Content-Type: text/plain; charset=us-ascii Dear All: I am seeking advice from someone who has experience with fixing and paraffin embedding rat muscle tissue. We want to dissect out muscles, fix them, embed in paraffin and then do HE and immunostaining. Our first attempts, which involved immersion fixation in 4% paraformaldehyde, resulted in either very poor fixation or the tissue got cooked in the tissue processor. There were parts of the muscle that were hardened and completely disrupted. We suspect poor fixation, perhaps due to incomplete penetration? However, if possible, we would like to avoid having to fix via perfusion. Are there any good tricks? Please email me off the listserv. Thanks, Sarah Pixley sarah.pix...@uc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: rat muscle fixation
Thank you Dr. Wright! We have full IACUC approval for perfusion and for all our procedures, and we have done a lot of paraformaldehyde perfusion fixation. It is just that for this study, we would like to avoid it, since it might interfere with other aspects of the experiment. So, we were wondering if there were any methods of fixing muscles that might get around the need for perfusion. For example, some studies add calcium to paraformaldehyde fixative and I was wondering if that might help preserve muscle better. We are working with the gastrocnemius muscle from adult rats, so it is quite thick (~1 cm diameter). I know that it may not be possible to get good immersion fixation for this big a piece of tissue. But I thought I might ask! Also, we could cut slabs of the muscle and then fix. Thanks, Sarah ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ASCP HT exam
Congrats on passing! What can I expect when I take it? Was it horrible? Kaiser regional in Berkeley are always hiring, usually night shift. They are union. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Billing IHC on MOHS
The following is the response I recived from a coding specialist at the
American Academy of Dermatology. I am trying not to be concerned that the
reference is 6 years old but I think it clears up what we thought to be true.
88342 for IHC
88314 other “special stains”
Here is the description for 88314 according to November 2006 cpt Assistant
article, the companion piece to the AMA CPT Code Book.
The work of processing and interpreting one routine stain is included in the
procedure 17311 javascript:cptaaCPTPopup('17311') - 17315
javascript:cptaaCPTPopup('17315') . This stain is usually hematoxylin and
eosin, or toluidine blue. If other special stains are necessary after one
routine stain, then the code for special stains may be used (88314
javascript:cptaaCPTPopup('88314') ) as well as immunoperoxidase stains (88342
javascript:cptaaCPTPopup('88342') ) or decalcification procedures (88311
javascript:cptaaCPTPopup('88311') ). Special stains are not typically used
and in most Mohs practices are of low frequency. Each stain is reported only
once per block, not per slide or per layer (stage).
AMA CPT definition of a Block: Tissue flattened by cutting into pieces,
embedded, and frozen in mounting medium used by histotechnologists to embed
tissue for frozen sections.
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[Histonet] billing IHC on Mohs
Mohs or not 88314 is for a special stain. They call this histo chemical because chemical reactions take place in the tissue. This is not IHC. If you want to bill for IHC on frozen sections you bill 88342. I just lloked this up in my trusty coding guide. Hope that helps or adds to the controversy as the case may me. Andrea Conard, HT(ASCP), QIHC Supervisor Anatomic Pathology AtlantiCare Reginonal Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Billing IHC on MOHS
Great team work! Job well done and a absolute answer is given. Thank you From: Carol Torrence ctorre...@kmcpa.com To: 'Kim Donadio' one_angel_sec...@yahoo.com Cc: 'Weems, Joyce K.' joyce.we...@emoryhealthcare.org; 'Ingles Claire' cing...@uwhealth.org; histonet@lists.utsouthwestern.edu Sent: Tuesday, June 19, 2012 2:10 PM Subject: RE: [Histonet] Billing IHC on MOHS The following is the response I recived from a coding specialist at the American Academy of Dermatology. I am trying not to be concerned that the reference is 6 years old but I think it clears up what we thought to be true. 88342 for IHC 88314 other “special stains” Here is the description for 88314 according to November 2006 cpt Assistant article, the companion piece to the AMA CPT Code Book. The work of processing and interpreting one routine stain is included in the procedure 17311- 17315. This stain is usually hematoxylin and eosin, or toluidine blue. If other special stains are necessary after one routine stain, then the code for special stains may be used (88314) as well as immunoperoxidase stains (88342) or decalcification procedures (88311). Special stains are not typically used and in most Mohs practices are of low frequency. Each stain is reported only once per block, not per slide or per layer (stage). AMA CPT definition of a Block:Tissue flattened by cutting into pieces, embedded, and frozen in mounting medium used by histotechnologists to embed tissue for frozen sections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Macrophage stain
Hi all, I would like to stain for macrophages in mice aorta (5um frozen sections). Would you have suggestions on which stains to use and perhaps share the protocol? Thanks in advance, Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9936 6676 T (03 9936 6794) E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au www.mcri.edu.au http://www.mcri.edu.au/ This e-mail and any attachments to it (the Communication) are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email __ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Billing IHC on MOHS
Well, I don't know if that settles that. I haven't responded, because I have not worked for a Mohs dermatopahtologist who runs Immunos (I have worked at numerous Mohs laboratories), however, this explanation is contradictory. Each stain is reported only once per block, not per slide or per layer (stage). Yet the definition of a block, Tissue flattened by cutting into pieces, embedded, and frozen in mounting medium used by histotechnologists to embed tissue for frozen sections. Every stage represent a new block in which slides are cut. These two statements are contradictory and need clarification. Now, my own opinion (again I have talked with my dermatopathologist and billing specialist and they are as lost as we) is that by definition, Mohs is a frozen section diagnosis that must be made by the surgeon (i.e., for a Mohs to be a mohs the surgeon removing the tissue must diagnose the tissue -- look it up). Every section taken, at every stage is a separate block of the same case. In the event you can charge immunos per case, only one charge can be made. If it can be shown that immunos can be charged per block (per the definition below), every immuno on every block from every stage can be charged. Now for the practicality -- we always start questions like this because medicare sets standards for billing that other insurance companies then adopt. We should NEVER ask, what can we charge for, but should always ask, what work did we do that it is fair for a patient to pay for. Ignore what medicare and insurance companies say, bill clients for the work we perform and for the results they get. How much more raw cost is there in staining two Mohs blocks with the same immuno? Is it fair to charge a patient double the amount for MUCH less than twice the work? Will Chappell HTL(ASCP), QIHC On Jun 19, 2012, at 9:15 PM, Kim Donadio wrote: Great team work! Job well done and a absolute answer is given. Thank you From: Carol Torrence ctorre...@kmcpa.com To: 'Kim Donadio' one_angel_sec...@yahoo.com Cc: 'Weems, Joyce K.' joyce.we...@emoryhealthcare.org; 'Ingles Claire' cing...@uwhealth.org; histonet@lists.utsouthwestern.edu Sent: Tuesday, June 19, 2012 2:10 PM Subject: RE: [Histonet] Billing IHC on MOHS The following is the response I recived from a coding specialist at the American Academy of Dermatology. I am trying not to be concerned that the reference is 6 years old but I think it clears up what we thought to be true. 88342 for IHC 88314 other “special stains” Here is the description for 88314 according to November 2006 cpt Assistant article, the companion piece to the AMA CPT Code Book. The work of processing and interpreting one routine stain is included in the procedure 17311- 17315. This stain is usually hematoxylin and eosin, or toluidine blue. If other special stains are necessary after one routine stain, then the code for special stains may be used (88314) as well as immunoperoxidase stains (88342) or decalcification procedures (88311). Special stains are not typically used and in most Mohs practices are of low frequency. Each stain is reported only once per block, not per slide or per layer (stage). AMA CPT definition of a Block:Tissue flattened by cutting into pieces, embedded, and frozen in mounting medium used by histotechnologists to embed tissue for frozen sections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Macrophage stain
Serotec has two antibodies that will work a F4/80 and CD68, both are rat anti-mouse. We have used them in the same application that you will be using (mouse aorta) with good success. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax l...@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniela Bodemer [daniela.bode...@mcri.edu.au] Sent: Tuesday, June 19, 2012 7:30 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Macrophage stain Hi all, I would like to stain for macrophages in mice aorta (5um frozen sections). Would you have suggestions on which stains to use and perhaps share the protocol? Thanks in advance, Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9936 6676 T (03 9936 6794) E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au www.mcri.edu.au http://www.mcri.edu.au/ This e-mail and any attachments to it (the Communication) are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email __ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Macrophage stain
Hi. all Let me add another question linked to marcophage staining. So far we have done macrophage staining several times with F4/80, and in most of the cases IHC images were quite beautiful, for liver, spleen, adipose tissue and so on. BUT, we have not succeeded in staining lung alveolar macrophages One of my friends(pathologist) told me that F4/80 was not good for alveolar macrophage but he also don't know how to deal with this problem. Does anyone know what is appropriate antibody for staining lung alveolar macrophage? Thanks in advance Hiro 2012/6/20 Elizabeth Chlipala l...@premierlab.com: Serotec has two antibodies that will work a F4/80 and CD68, both are rat anti-mouse. We have used them in the same application that you will be using (mouse aorta) with good success. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax l...@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniela Bodemer [daniela.bode...@mcri.edu.au] Sent: Tuesday, June 19, 2012 7:30 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Macrophage stain Hi all, I would like to stain for macrophages in mice aorta (5um frozen sections). Would you have suggestions on which stains to use and perhaps share the protocol? Thanks in advance, Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9936 6676 T (03 9936 6794) E daniela.bode...@mcri.edu.au mailto:firstname.surn...@mcri.edu.au www.mcri.edu.au http://www.mcri.edu.au/ This e-mail and any attachments to it (the Communication) are, unless otherwise stated, confidential, may contain copyright material and is for the use only of the intended recipient. If you receive the Communication in error, please notify the sender immediately by return e-mail, delete the Communication and the return e-mail, and do not read, copy, retransmit or otherwise deal with it. Any views expressed in the Communication are those of the individual sender only, unless expressly stated to be those of Murdoch Childrens Research Institute (MCRI) ABN 21 006 566 972 or any of its related entities. MCRI does not accept liability in connection with the integrity of or errors in the Communication, computer virus, data corruption, interference or delay arising from or in respect of the Communication. P Please consider the environment before printing this email __ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
