Re: [Histonet] Negative Reagent Control
Our Prayers have been answered!!! Richard Cartun rcar...@harthosp.org 7/17/2012 7:04 PM Most of you know that I have been advocating for the elimination of the Negative Reagent Control when using a non-avidin-biotin detection system (polymer) in immunohistochemical testing. In my opinion, it is a waste of healthcare dollars and, more importantly, precious patient specimen. Last year at the NSH IHC Forum in Denver I met James Dvorak, MT(ASCP) from the College of American Pathologists. We had a long discussion about this and he agreed to support my position within CAP. With the support of James, and the help of Dr. Regan Fulton (from the CAP IHC Committee), the wording on the CAP Anatomic Pathology checklist for question ANP.22570 will be changed. The new wording includes the following sentence: Immunohistochemical tests using polymer-based detection systems (biotin-free) are sufficiently free of background reactivity to obviated the need for a negative reagent control and such controls may be omitted at the discretion of the laboratory director. I announced this change at the 2012 NSH IHC/ISH Forum this past weekend in Windsor, CT to loud applause. This one change stands to save the healthcare system millions of dollars. Thank you Jim and Dr. Fulton for making this happen! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Negative Reagent Control
What wonderful news Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Wednesday, July 18, 2012 7:26 AM To: Richard Cartun; Histonet Subject: Re: [Histonet] Negative Reagent Control Our Prayers have been answered!!! Richard Cartun rcar...@harthosp.org 7/17/2012 7:04 PM Most of you know that I have been advocating for the elimination of the Negative Reagent Control when using a non-avidin-biotin detection system (polymer) in immunohistochemical testing. In my opinion, it is a waste of healthcare dollars and, more importantly, precious patient specimen. Last year at the NSH IHC Forum in Denver I met James Dvorak, MT(ASCP) from the College of American Pathologists. We had a long discussion about this and he agreed to support my position within CAP. With the support of James, and the help of Dr. Regan Fulton (from the CAP IHC Committee), the wording on the CAP Anatomic Pathology checklist for question ANP.22570 will be changed. The new wording includes the following sentence: Immunohistochemical tests using polymer-based detection systems (biotin-free) are sufficiently free of background reactivity to obviated the need for a negative reagent control and such controls may be omitted at the discretion of the laboratory director. I announced this change at the 2012 NSH IHC/ISH Forum this past weekend in Windsor, CT to loud applause. This one change stands to save the healthcare system millions of dollars. Thank you Jim and Dr. Fulton for making this happen! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IL5
Does anyone on the net do or know of a lab that does IL-5 IHC for clinical/diagnostic purposes? TIA Luis Chiriboga Ph.D, Director OCS Experimental Pathology IHC Core Lab NYU School of Medicine Medical Science Building Room 124 646-501-6934 luis.chirib...@nyumc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Negative Reagent Control
Outstanding!! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, July 17, 2012 7:05 PM To: Histonet Subject: [Histonet] Negative Reagent Control Most of you know that I have been advocating for the elimination of the Negative Reagent Control when using a non-avidin-biotin detection system (polymer) in immunohistochemical testing. In my opinion, it is a waste of healthcare dollars and, more importantly, precious patient specimen. Last year at the NSH IHC Forum in Denver I met James Dvorak, MT(ASCP) from the College of American Pathologists. We had a long discussion about this and he agreed to support my position within CAP. With the support of James, and the help of Dr. Regan Fulton (from the CAP IHC Committee), the wording on the CAP Anatomic Pathology checklist for question ANP.22570 will be changed. The new wording includes the following sentence: Immunohistochemical tests using polymer-based detection systems (biotin-free) are sufficiently free of background reactivity to obviated the need for a negative reagent control and such controls may be omitted at the discretion of the laboratory director. I announced this change at the 2012 NSH IHC/ISH Forum this past weekend in Windsor, CT to loud applause. This one change stands to save the healthcare system millions of dollars. Thank you Jim and Dr. Fulton for making this happen! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] any news on CD4, CD8 IHC on FFPE mouse tissue?
I'm wondering if anyone has recommendations for CD4 and CD8 antibodies for FFPE mouse tissue. Looking through the archives I see there was only success on frozen tissues. Has anything changed recently?? Or should I stick with CD3. Thanks, Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
I work in a small lab (office bldg) that has been turned into histolab. We have a portable work station with a vent to out side. (roof) I also have my VIP processor in this room which has the filter carbon (activated) on the processor. Where we have an issue is , on very windy days there seems to be a down draft that happens causing fumes to go into other offices (not ours). I have been looking into the air filter system by leica and one mercedes sells. They have a filter that can be changed every 6 months or so. Has anyone used these with any sort of success rate? Kate Mendell Histopathology/Lab Manager HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmend...@goldbergmd.netmailto:kmend...@goldbergmd.net www.cosmesticdermcenter.comhttp://www.cosmesticdermcenter.com/ PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Histology Job Update. Summertime in the South is Sizzlin hot and it's not just the temps!
Hi Histonetters!! I hope everyone is having a great day. Are you keeping cool on these hot summer days? Summertime in the South is Sizzlin hot and it's not just the temps!! I have some great new opportunities to tell you about: I have full time permanent positions with some of the best employers in the South. They offer competitive salaries, great benefits and awesome crews to work with. Here is a list of the Sizzlin Summer Stuff! Dermpath HistoTech - Atlanta, GA Histology Tech evenings - Charlotte, NC Histology Tech nights - Charlotte, NC Histology Tech Days - Bristol, TN Histology Tech Nights - Lafayette, LA If you have any questions or know someone who might be interested please contact me. I would love to tell you about these opportunities or write another referral check like I did today if you have a friend who might be interested. I hope to hear from you soon. In the meantime grab some shade and a cool drink! Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com http://www.facebook.com/PamBarkerRELIA /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NSH S/C
I am flying into Bellingham, WA on Friday Sept. 28th to attend the NSH S/C (much cheaper than flying into Vancouver) at 2:38pm and am looking to share a limo to Vancouver with 5 or 6 people. If you are interested please email me. If I get enough interest, I will follow up and see if that is feasible. Mequita Praet mdpr...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Image Analysis for Fat Tissue
Hi Ateret, You've got a few choices, some of which are free and some of which are quite expensive. In many soft tissue staining protocols, adipocytes show up as white, oval, void spaces. Which is good! Software like ImageJ http://rsbweb.nih.gov/ij/, or its more integrated variant FIJI http://fiji.sc/wiki/index.php/Fiji (which stands for Fiji is Just Image J) is an excellent place to start. They have very good and easy to use measurement tools for area and number. Unfortunately, the topic takes a turn for the complex. Cell number and cell size (i.e. cell volume) can be subject to systematic bias due to section orientation, section thickness, and related parameters. This may cause under or over estimation of the data. This brings us to Stereologyhttp://en.wikipedia.org/wiki/Stereology, the science of estimating data from 2d cross-sections of 3d structures. Say for example, you're sectioning at 5 microns. The 2d profiles (i.e. cross-sections) of a large adiopcyte will appear on many sections, but the 2d profile of a small adiopcyte will show up only once or twice. In this way, if you count all the adipocyte profiles you see, you could be overestimating the number of adipocytes. You could be counting some adiopocytes more than once. There are standard techniques for estimating the total number of cells in a region and the mean volume of a group of cells. The Optical Fractionator estimates the total number of cells. The Rotator estimates mean cell volume. These tools are found in most stereology systems, commercial or free. The drawback to these methods are that they require specialized commercial software and a microscope capable of monitoring real-time position in X, Y, and Z. This is commonly done with either a motorized stage or encoded stage. Some practical alternatives you could implement with ImageJ may be: 1. Measure Adipose Volume. That is the area of adipose tissue measured over a set of serial sections. The Delesse principlehttp://en.wikipedia.org/wiki/Stereology(again from Stereology) says that area fractions are proportional to volume fractions in a set of serial sections. So no special hardware or random sampling is required. 2. Take care to only count nucleated adipocyte profiles. This helps minimize the problem of overcounting in serial sections. 3. Count adipocytes in sections that are not adjacent. Meaning, cut 5 micron sections but make sure the sections you count are far enough apart that you won't see the cross-sections from the same adipocyte. 4. As far as measuring the volume of individual adipocytes, you might be able use the shortest diameter of the nucleated adipocyte profiles in you see in your sections. This is a tricky one and very much subject to the shape of your adipocyte population. As always, you'll do well to mimic the methods of those that have performed similar work in the past. This will make it easier for reviewers to understand your methods and comment constructively on your paper. You might find this Google Scholar searchhttp://scholar.google.com/scholar?as_ylo=2008q=adipocyte+volume+numberhl=enas_sdt=0,43helpful. It covers references to adipocyte volume and adipocyte number since 2008. Best of Luck, Nathanael -- Nathanael Reveal, CEO BIOQUANT Image Analysis Corporation 5611 Ohio Avenue, Nashville, TN 37209 phone: 615-350-7866, toll free: 800-221-0549, fax: 615-350-7282 email: nathan...@bioquant.com, web: www.bioquant.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hold IHC overnight
Just found out I have to go to a meeting in 2 hours for the rest of the day (I haven't even eaten lunch yet). I do IHC manually, they just got done cooling off from HIER...I can store them overnight in buffer right? Just double checking myself as this run is an antibody validation run... -S- Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Image analysis for fat tissue
Hi, That is a pretty common kind of measurement. You will need a digital camera attached to your microscope, and a stage micrometer. You can then use the free software Image-J to get area measurements. If you have some extra money Image-Pro software from Media Cybernetics is the deluxe way to go. Jerry Ricks Research Scientist University of Washington Department of Pathology From: ater...@migal.org.il To: histonet@lists.utsouthwestern.edu Date: Wed, 18 Jul 2012 11:20:42 + Subject: [Histonet] Image analysis for fat tissue Dear Histonet members I'm interested in image analysis of mice fat tissue, I would like to quantify number Of cells per area and average cell size. Does anyone know a software (+instructions/ lucid manual) which does that? Many thanks! -- Best regards -- Ateret Shabtay-Yanai Migal- Galilee Technology Center Southern Industrial Zone- kiryat Smona P.O.B 831 Kiryat Shmona 11016 Tel: +972-46955002 Mobile: +972-547946460 Email: ater...@migal.org.il ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Black Biopsy Paper
I am wondering if anyone out there is using black biopsy paper to give to offices to place the biopsy on prior to putting it into formalin? It looks kind of like construction paper. If you are using this can you please give me a list of pros and cons associated with it? Thanks, Becky Disclaimer Note: The information in this message with any attachment to it, may be privileged and protected from disclosure. If the reader of this message is neither the intended recipient, nor an employee or agent responsible for delivering this message to the intended recipient, then you are hereby notified that any dissemination, distribution, unauthorized use, or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Hold IHC overnight
I vote No - this run would be valid only if you held all future slides overnight after HIER. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Wednesday, July 18, 2012 11:44 AM To: histonet Subject: [Histonet] Hold IHC overnight Just found out I have to go to a meeting in 2 hours for the rest of the day (I haven't even eaten lunch yet). I do IHC manually, they just got done cooling off from HIER...I can store them overnight in buffer right? Just double checking myself as this run is an antibody validation run... -S- Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Black Biopsy Paper
Becky, We have used this paper. What I found is that when submersed in the formalin the black dye leaches out of the paper and the portion of the tissue touching the paper turned somewhat black. These were tissues left on the paper and in formalin overnight. Since I never processed that tissue I don't know if the black washes out during processing. Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rebecca LeVier Sent: Wednesday, July 18, 2012 2:26 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Black Biopsy Paper I am wondering if anyone out there is using black biopsy paper to give to offices to place the biopsy on prior to putting it into formalin? It looks kind of like construction paper. If you are using this can you please give me a list of pros and cons associated with it? Thanks, Becky Disclaimer Note: The information in this message with any attachment to it, may be privileged and protected from disclosure. If the reader of this message is neither the intended recipient, nor an employee or agent responsible for delivering this message to the intended recipient, then you are hereby notified that any dissemination, distribution, unauthorized use, or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Hold IHC overnight
I agree On Jul 18, 2012, at 2:35 PM, Goins, Tresa tgo...@mt.gov wrote: I vote No - this run would be valid only if you held all future slides overnight after HIER. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Wednesday, July 18, 2012 11:44 AM To: histonet Subject: [Histonet] Hold IHC overnight Just found out I have to go to a meeting in 2 hours for the rest of the day (I haven't even eaten lunch yet). I do IHC manually, they just got done cooling off from HIER...I can store them overnight in buffer right? Just double checking myself as this run is an antibody validation run... -S- Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Hold IHC overnight
But only because it's a validation run. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erin and Jesse Sent: Wednesday, July 18, 2012 3:35 PM To: Goins, Tresa Cc: histonet Subject: Re: [Histonet] RE: Hold IHC overnight I agree On Jul 18, 2012, at 2:35 PM, Goins, Tresa tgo...@mt.gov wrote: I vote No - this run would be valid only if you held all future slides overnight after HIER. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Wednesday, July 18, 2012 11:44 AM To: histonet Subject: [Histonet] Hold IHC overnight Just found out I have to go to a meeting in 2 hours for the rest of the day (I haven't even eaten lunch yet). I do IHC manually, they just got done cooling off from HIER...I can store them overnight in buffer right? Just double checking myself as this run is an antibody validation run... -S- Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Hold IHC overnight
No you can't do that. If it were just a research IHC then you could give it a whirl, write down what you did and often it would work just fine depending on antigen and antibody. But if you are validating an antibody for clinical lab you have to document you follow GLP SOP every time else you messed up. There's no getting around it. You are just going to have to run the IHC stain again. Jerry Ricks From: sdys...@mirnarx.com To: histonet@lists.utsouthwestern.edu Date: Wed, 18 Jul 2012 17:44:12 + Subject: [Histonet] Hold IHC overnight Just found out I have to go to a meeting in 2 hours for the rest of the day (I haven't even eaten lunch yet). I do IHC manually, they just got done cooling off from HIER...I can store them overnight in buffer right? Just double checking myself as this run is an antibody validation run... -S- Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] automated H+E and cover slipper
Hi All, I will be trying out new H+E autostainers and cover slippers soon. Does anyone have a favorite, and why? Thanks for your responses. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] automated H+E and cover slipper
Leica Autostainer XL with CV5030 coverslipper and transfer station. This has been a real timesaver for us. It automatically moves the slides from the stainer to the coverslipper. It has been relatively trouble free. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Hardin Sent: Wednesday, July 18, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] automated H+E and cover slipper Hi All, I will be trying out new H+E autostainers and cover slippers soon. Does anyone have a favorite, and why? Thanks for your responses. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] automated H+E and cover slipper
We have a Sakura Tissue Tek Prisma and Glas coverslipper we really like it. It's a workhorse. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Hardin Sent: Wednesday, July 18, 2012 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] automated H+E and cover slipper Hi All, I will be trying out new H+E autostainers and cover slippers soon. Does anyone have a favorite, and why? Thanks for your responses. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] automated H+E and cover slipper
I second on that! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Wednesday, July 18, 2012 1:44 PM To: Joe Hardin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] automated H+E and cover slipper Leica Autostainer XL with CV5030 coverslipper and transfer station. This has been a real timesaver for us. It automatically moves the slides from the stainer to the coverslipper. It has been relatively trouble free. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Hardin Sent: Wednesday, July 18, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] automated H+E and cover slipper Hi All, I will be trying out new H+E autostainers and cover slippers soon. Does anyone have a favorite, and why? Thanks for your responses. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
It is added to formaldehyde to prevent polymerization of the formaldehyde. Bader Siddiki bade...@gmail.com Sent by: histonet-boun...@lists.utsouthwestern.edu 07/17/2012 01:44 PM To Rene J Buesa rjbu...@yahoo.com cc histonet Histonet@lists.utsouthwestern.edu, Kelly Boyd kdboydhi...@yahoo.com Subject Re: [Histonet] (no subject) Hello Methanol is added to reagent alcohol (ethyl alcohol) so that people do not use for drinking purpose and for tax purposes. Alcohol (ethyl alcohol) has federal tax just like liquor. One can buy pure alcohol, but you have keep inventory and make sure it is not misused. Bader On Tue, Jul 17, 2012 at 1:28 PM, Rene J Buesa rjbu...@yahoo.com wrote: To prevent oxidation of those 2 reagents René J. From: Kelly Boyd kdboydhi...@yahoo.com To: histonet Histonet@lists.utsouthwestern.edu Sent: Tuesday, July 17, 2012 1:16 PM Subject: [Histonet] (no subject) Since there is discussion of formalin and methanol, I would like to ask everyone.Why would a vendor add methanol to their reagent alcohol and their 10% buffered formalin? What are the advantages/disadvantages to the tissue especially for processing? Kelly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- If any Q's please feel free to contact us Have a nice day/weekend Mit freundlichen Grüßen / With Kind Regards / avec l'aimable ce qui concerne Met vriendelijke groeten 種とについて Bader Bader B Siddiki, PhD Executive director, Research and development ImmunoBioScience Corp. (IBSC) Phone: + 1 425 367 4601 Fax: + 1 425 367 4817 cell (mobile) phone: + 1 425 314 0199 e-mail address: bade...@gmail.com Web site: www.ImmunoBioScience.Com Marketing: phone: + 1 650 343 IBSC (4272) E-mail: anitai...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] automated H+E and cover slipper
Sakura Prisma w/ tape cover slip. It also is available w/ glass cover slip. Reliable, fast and a workhorse. William DeSalvo, B.S., HTL(ASCP) From: bdebrosse-se...@isisph.com To: sandra.harris...@va.gov; har...@oncology.wisc.edu; histonet@lists.utsouthwestern.edu Date: Wed, 18 Jul 2012 14:18:53 -0700 Subject: RE: [Histonet] automated H+E and cover slipper CC: I second on that! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Wednesday, July 18, 2012 1:44 PM To: Joe Hardin; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] automated H+E and cover slipper Leica Autostainer XL with CV5030 coverslipper and transfer station. This has been a real timesaver for us. It automatically moves the slides from the stainer to the coverslipper. It has been relatively trouble free. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Hardin Sent: Wednesday, July 18, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] automated H+E and cover slipper Hi All, I will be trying out new H+E autostainers and cover slippers soon. Does anyone have a favorite, and why? Thanks for your responses. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
