[Histonet] symphony another question
Hi! A question for Symphony-users. Do you enjoy working with the Symphony? Is it really a change to the better in comparison to other combined staining-coverslipping devices? I don't ask for quality or patient security - just for user concerns. Did it ever happen, that a run-error turned the system down (like it sometimes does with our Ultra)? What are the consequences? Just curious. Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] (no subject)
... thinking about muscle packed little woman in labcoat tearing one phone book after another ... : ) Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Rena Fail Gesendet: Dienstag, 31. Juli 2012 23:33 An: Paula Pierce Cc: Histonet Betreff: Re: [Histonet] (no subject) Can't beat phone books. Cheap, torn in half they are wider than a kimwipe, you're recycling, and providing therapy in the form of stress relief obtained tearing up phone books. Rena On Tue, Jul 31, 2012 at 2:23 PM, Paula Pierce cont...@excaliburpathology.com wrote: Greetings, does anyone know of a less expensive substitute for Kim-wipes? Paula K. Pierce, HTL(ASCP)HT President Excalibur Pathology, Inc. 8901 S. Santa Fe, Suite G Oklahoma City, OK 73139 405-759-3953 Lab 405-759-7513 Fax www.excaliburpathology.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] calcium buildup
Hi, Does anyone know of a way to remove calcium buildup on the wash nozzles in the water reservoirs. I have the Tissue-Tek DRS 2000. Thank you. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston,TX 77030 832-355-6524 832-355-6812 (fax) [THI Celebrates 50 Years]http://www.texasheart.org/AboutUs/History/index.cfm Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org www.texasheart.orgwww.texasheart.orghttp://www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI on Facebook]http://www.facebook.com/Texas.Heart.Institute[THI on Twitter]http://twitter.com/Texas_Heart[THI on YouTube]http://www.youtube.com/TexasHeartInstitute[THI's Flickr page]http://www.flickr.com/photos/texasheart/sets/ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] calcium buildup
Hi Molinari I used microbiology long cotton wool swabs. If they are short extend them by burning the plastic ends and joining them while they are hot. Make sure the join is straight. Unscrew the nozzles from the machine and push the swabs in and out to clean and open the holes. If the blockage is hard u may use any small sharp metal pins to unblock the holes. Good luck. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: 01 August 2012 12:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] calcium buildup Hi, Does anyone know of a way to remove calcium buildup on the wash nozzles in the water reservoirs. I have the Tissue-Tek DRS 2000. Thank you. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston,TX 77030 832-355-6524 832-355-6812 (fax) [THI Celebrates 50 Years]http://www.texasheart.org/AboutUs/History/index.cfm Betsy Molinari Senior Histology Research Technician 832-355-6524 | bmolin...@texasheart.orgmailto:bmolin...@texasheart.org www.texasheart.orgwww.texasheart.orghttp://www.texasheart.org TEXAS HEART INSTITUTE 6770 Bertner Ave. MC 1-283 | Houston, TX 77030 [Receive electronic news from THI]https://secure3.convio.net/thi/site/SPageNavigator/GlobalSiteOptInPage.html[THI on Facebook]http://www.facebook.com/Texas.Heart.Institute[THI on Twitter]http://twitter.com/Texas_Heart[THI on YouTube]http://www.youtube.com/TexasHeartInstitute[THI's Flickr page]http://www.flickr.com/photos/texasheart/sets/ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient, or an authorized representative of the intended recipient, you are hereby notified that any review or dissemination or copying of this e-mail and its attachments, if any, or the information contained herein, is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Proteolytic enzyme pretreatment before immunostaining
The proteolytic activity of the enzymes can be non-specific (Pronase) or specific (peptide bonds at lysine and arginine residues, Trypsin). The effect of formalin fixation is not identified, so we always assess Pepsin, Trypsin and Pronase along with heat retrieval methods regardless of what the supplier data sheet recommends. Tresa Dear Histonetters, Could anyone explain about the difference between proteolytic enzymes for immuostaining? We use enzyme pretreatment rarely nowadays, and apart from some ready-to-use one (Dako's Proteinase K), I have used Protease (Sigma) previously when I did manual staining. At the moment I am using Leica's BondMax autostainer and their enzyme pretreatment kit (Trypsin ??, usually one drop per 7 ml vial). I know the pretreatment condition would be affected by the concentration of enzymes, pH, temperature, incubation time etc. My question is that do they have different mode of action on tissues? I am helping a research project and our antibody includes various clones of Integrin 6 subunit and uPAR. I have tried enzyme pretreatment with autostainer and manual staining with Proteinase K (Dako). It seems that some antibodies work better with certain enzymes. I mean that some antibodies work well after BondMax enzyme treatment, but some antibody works better with proteinase K pretreatment manually. I am using the same polymer detection system (Leica Microsystem) for both methods. I would like to find an enzyme which works for both of our antibodies at the same time. Thank you. Young Kwun Senior Hospital Scientist Immunohistochemistry Dept. of Anatomical Pathology Concord Repatriation General Hospital Concord NSW 2139 Australia 02-9767-6075 (Tel) 02-9767-8427 (Fax) young.k...@sswahs.nsw.gov.au ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Position - IHC Reagent and Antibody Sales
Good Morning Histonet, Our client has recently opened a new expansion sales opportunity and I wanted to reach out regarding a few specifics and see if anyone would be interested in learning more. Our client is a global leader in Histology and IHC has recently opened a new expansion sales opportunity in the Midwest Region to focus on driving IHC Reagent and Antibody sales. We are searching for a histology professional who has a strong background in IHC and antibodies that would be interested in working in a team sales environment. Though sales experience is a plus, this position does not require previous sales experience and this would be outstanding opportunity for someone looking to break into sales that has a strong histology background. The ideal location for the position would be in the greater Minneapolis area, however we will consider candidates in the region (MN, ND, SD, IA, NE, KS). The position offer s strong package including a base salary, competitive commission structure, car allowance, gas card, cell phone, laptop computer, and full benefits. If you or anyone you may know would be interested in the position, please contact me directly at m...@personifysearch.com to learn more. Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 50mm x 100mm staining rack available
Hi histoland, Thanks for all the help a few weeks ago. I found a commercially available 15 place staining rack for 50mm x 100mm slides. It works perfectly and I recommend it to anyone needing to stain slides of that size. It's a bit difficult to find on the web, and since I don't believe in blatant advertising message me if you are interested and I will send you the link. Cheers! Marc DeCarlo ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Expert Level Cutting and Embedding
I see Jay Lundgren's point, but it seems to me that the present shortage of histotechnologists makes HistoCare's advertisement of their services entirely appropriate for HistoNet. Bob Richmond Samurai Pathologist Knoxville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Secondary antibody causing nuclear staining
Eva, Have you gotten your problem resolved yet? I was curious as to what heat method you are using? Are you using a microwave? I'm sorry to be asking so many questions and not getting back to you quickly. I am thinking if you have irregular positive staining in the same Negative control tissue on different days that its a technique issue. Maybe the microwave is heating irregular? Ive also seen when using a microwave that I got better results when I would make sure they dont evaporate when transferring to next reagent and I circulated the buffer with a pipette in between the heating process. Now if your using a steamer some people still do, dont laugh then perhaps if its the second batch the retreival is hotter than for the first batch causing overstaining for that second batch?. Is there any possibliltiy at all some of the slides could be drying out during the staining process? I have seen nuclear artifact in some cases this happened to. Are you making up your DAB everyday, there are some DAB's that you have to make up and they get old quickly and dont stain as well later on. So many questions, sorry. I'd love to help you if you havnt figured this out yet. Please feel free to email me. Kim D From: Eva Permaul e...@georgetown.edu To: Reynolds, Donna M dreyn...@mdanderson.org; histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, July 25, 2012 10:59 AM Subject: Re: [Histonet] RE: Secondary antibody causing nuclear staining I do see positive nuclei in the NC. That is what I am asking about. I know I could switch methods but my question is also why if it is happening is it not as strong all the time? Why are the cells very light one day and dark the next? What is causing them to stain? Just curious is all. Eva On Wed, Jul 25, 2012 at 10:04 AM, Reynolds,Donna M dreyn...@mdanderson.orgwrote: If you are running a negative control (no primary)with your ABC staining wouldn't you see the same nuclear labeling in the NC ? Thus alerting you to false staining and indicating that you should try a HRP conjugated secondary or use a polymer system. Good discussion thank Tony. Donna Reynolds HT(ASCP) Chief Histologist IHC Lab -Original Message- I understand the point about the biotin and I should have said that when using the ABC method we have taken to always using an avidin/biotin blocking kit. We are using biotinylated secondary antibodies from Vector. I have seen the same problem occur in our anti-mouse, anti-rabbit and anti-goat. In my last run I had stomach fundus as well as skin melanoma, both had pos.nuclei in the negative (no primary). In another run I had colon ca and breast ca, the breast ca had fewer pos. nuclei than the colon ca but they were still there. Some days the positive nuclei are stronger in a sample that was just weakly positive before. Just want to understand what it is and what effects it. Thank you all for your ideas. Eva Permaul Georgetown University On Mon, Jul 23, 2012 at 7:16 PM, Tony Henwood (SCHN) tony.henw...@health.nsw.gov.au wrote: I should have added that this was from the workshop notes on a Hypotheticals Workshop I ran last year at our Australian National Meeting. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Tuesday, 24 July 2012 9:00 AM To: 'Eva Permaul'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Secondary antibody causing nuclear staining? It is possible that this is due to Biotin nuclei where excess biotin is found in the nuclei of some cells, see below: Optically clear nuclei have been reported in endometrial epithelium associated with first and second trimester abortions (Sickel di Sant'Agnese 1994). Optically clear nuclei have also been found in different types of tissues of diverse organs such as ovary, thyroid and lung (Nakatani et al 1994, Mount Cooper 2001). The optically clear nuclei contain excess biotin. Endogenous biotin immunoreactivity is generally not visualized in formalin fixed, paraffin-embedded tissues unless a heat-induced antigen retrieval step has been introduced (Mount Cooper 2001). In this placental section, optically clear nuclei (containing biotin) bind to the streptavidin of the ABC technique giving a reaction similar to that seen with CMV containing cells. If a polymer method (or even the original Sternberger's PAP method) is used then this anomalous staining will disappear, thus allowing confident demonstration of CMV
AW: [Histonet] RE: Proteolytic enzyme pretreatment before immunostaining
I think formalin fixation does something similar to enzyme-binding as to antibody-binding, if the enzyme has a specific binding site. The aminoacids are masked by methylol-adducts and therefore the protein is protected against eg pepsin. So using combinated HIER and PIER is not only summarizing the effects. HIER renders PIER more effective and therefore more aggressive. Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Goins, Tresa Gesendet: Mittwoch, 01. August 2012 16:00 An: Young Kwun; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: Proteolytic enzyme pretreatment before immunostaining The proteolytic activity of the enzymes can be non-specific (Pronase) or specific (peptide bonds at lysine and arginine residues, Trypsin). The effect of formalin fixation is not identified, so we always assess Pepsin, Trypsin and Pronase along with heat retrieval methods regardless of what the supplier data sheet recommends. Tresa Dear Histonetters, Could anyone explain about the difference between proteolytic enzymes for immuostaining? We use enzyme pretreatment rarely nowadays, and apart from some ready-to-use one (Dako's Proteinase K), I have used Protease (Sigma) previously when I did manual staining. At the moment I am using Leica's BondMax autostainer and their enzyme pretreatment kit (Trypsin ??, usually one drop per 7 ml vial). I know the pretreatment condition would be affected by the concentration of enzymes, pH, temperature, incubation time etc. My question is that do they have different mode of action on tissues? I am helping a research project and our antibody includes various clones of Integrin 6 subunit and uPAR. I have tried enzyme pretreatment with autostainer and manual staining with Proteinase K (Dako). It seems that some antibodies work better with certain enzymes. I mean that some antibodies work well after BondMax enzyme treatment, but some antibody works better with proteinase K pretreatment manually. I am using the same polymer detection system (Leica Microsystem) for both methods. I would like to find an enzyme which works for both of our antibodies at the same time. Thank you. Young Kwun Senior Hospital Scientist Immunohistochemistry Dept. of Anatomical Pathology Concord Repatriation General Hospital Concord NSW 2139 Australia 02-9767-6075 (Tel) 02-9767-8427 (Fax) young.k...@sswahs.nsw.gov.au ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] counterstain for Alcian Blue (ph2.5)
Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: counterstain for Alcian Blue (ph2.5)
If I recall correctly Newcomer Supply and Poly Scientific both sell NFR. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig [dmcc...@ckha.on.ca] Sent: Wednesday, August 01, 2012 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] counterstain for Alcian Blue (ph2.5) Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Proteolytic enzyme pretreatment before immunostaining
When re-reading my e-mail, it does sound like we use heat and enzymatic retrieval together - we do not - we assess the effective of all retrieval methods used singly. Tresa -Original Message- From: Gudrun Lang [mailto:gu.l...@gmx.at] Sent: Wednesday, August 01, 2012 10:28 AM To: Goins, Tresa Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] RE: Proteolytic enzyme pretreatment before immunostaining I think formalin fixation does something similar to enzyme-binding as to antibody-binding, if the enzyme has a specific binding site. The aminoacids are masked by methylol-adducts and therefore the protein is protected against eg pepsin. So using combinated HIER and PIER is not only summarizing the effects. HIER renders PIER more effective and therefore more aggressive. Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Goins, Tresa Gesendet: Mittwoch, 01. August 2012 16:00 An: Young Kwun; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: Proteolytic enzyme pretreatment before immunostaining The proteolytic activity of the enzymes can be non-specific (Pronase) or specific (peptide bonds at lysine and arginine residues, Trypsin). The effect of formalin fixation is not identified, so we always assess Pepsin, Trypsin and Pronase along with heat retrieval methods regardless of what the supplier data sheet recommends. Tresa Dear Histonetters, Could anyone explain about the difference between proteolytic enzymes for immuostaining? We use enzyme pretreatment rarely nowadays, and apart from some ready-to-use one (Dako's Proteinase K), I have used Protease (Sigma) previously when I did manual staining. At the moment I am using Leica's BondMax autostainer and their enzyme pretreatment kit (Trypsin ??, usually one drop per 7 ml vial). I know the pretreatment condition would be affected by the concentration of enzymes, pH, temperature, incubation time etc. My question is that do they have different mode of action on tissues? I am helping a research project and our antibody includes various clones of Integrin 6 subunit and uPAR. I have tried enzyme pretreatment with autostainer and manual staining with Proteinase K (Dako). It seems that some antibodies work better with certain enzymes. I mean that some antibodies work well after BondMax enzyme treatment, but some antibody works better with proteinase K pretreatment manually. I am using the same polymer detection system (Leica Microsystem) for both methods. I would like to find an enzyme which works for both of our antibodies at the same time. Thank you. Young Kwun Senior Hospital Scientist Immunohistochemistry Dept. of Anatomical Pathology Concord Repatriation General Hospital Concord NSW 2139 Australia 02-9767-6075 (Tel) 02-9767-8427 (Fax) young.k...@sswahs.nsw.gov.au ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MOHS Tech
Hi, We are looking for MOHS tech and have an applicant that is not a certified histotech. Our MOHS techs are expected to gross tissues, which requires a certified tech for high complexity testing... according to CAP regulations. I was wondering what other MOHS techs are doing that are not certified technicians. I would really like to give this person a chance, but am afraid that I won't be able to because of the CAP standards. Do anyone have any references, resources, or information that could help me in this matter? Thank you, Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org http://www.mayoclinichealthsystem.org/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Expert Level Cutting and Embedding
Thanks for all the inquiries! And thanks Mr. Richmond! This is to provide clarity in response to some of the questions asked about HistoCare's service. We provide an experienced technician directly to your lab when a need arises such as when a regular employee has a planned absence like a pregnancy or surgery, or if you have a sudden change in staff from someone quitting or jury duty for example. You don't have to scramble to hurry up and hire the wrong person just to keep the lab running smoothly. The benefit is you get a strong, detailed, and reliable person to embed and cut and minimize the stress and workload on other FTEs. Also there is no long term commitment should your departmental needs change. In the interim you are not taking on additional stress from trying to figure out how to get the work done and you get to take your time to find the right replacement or wait until your regular employee returns. It is not our intent to offend any subscribers to histonet by posting our service. We are available on short notice, flexible with time and can work together on a budget that meets your needs. Please send inquiries and Technician requests to cont...@histocare.com. Thanks, Betty Smith, HistoCare Assistant ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: MOHS Tech
I would review the CLIA guidelines, which dictate minimum qualifications to perform certain tasks. http://wwwn.cdc.gov/clia/regs/subpart_m.aspx Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. [bauer.ka...@mayo.edu] Sent: Wednesday, August 01, 2012 1:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MOHS Tech Hi, We are looking for MOHS tech and have an applicant that is not a certified histotech. Our MOHS techs are expected to gross tissues, which requires a certified tech for high complexity testing... according to CAP regulations. I was wondering what other MOHS techs are doing that are not certified technicians. I would really like to give this person a chance, but am afraid that I won't be able to because of the CAP standards. Do anyone have any references, resources, or information that could help me in this matter? Thank you, Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org http://www.mayoclinichealthsystem.org/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: MOHS Tech
For grossing all you need are enough college hours in bio and chem. As to CLIA standards and that's what CAP follows if im correct. They do not have to be a certified histotech to gross tissue. Unless its required by state regulations. Requirements are 6 hours of biology and 6 hours of chemistry and then combination of 12 more hours in bio/chem. Total of 24 hours college credit Vanessa Perez Garcia Histology Supervisor Pathology Reference Lab 210-892-3746 210-892-3732 vpe...@pathreflab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Wednesday, August 01, 2012 12:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MOHS Tech Importance: Low Hi, We are looking for MOHS tech and have an applicant that is not a certified histotech. Our MOHS techs are expected to gross tissues, which requires a certified tech for high complexity testing... according to CAP regulations. I was wondering what other MOHS techs are doing that are not certified technicians. I would really like to give this person a chance, but am afraid that I won't be able to because of the CAP standards. Do anyone have any references, resources, or information that could help me in this matter? Thank you, Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.org http://www.mayoclinichealthsystem.org/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: counterstain for Alcian Blue (ph2.5)
As does Rowley Biochemical. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Wednesday, August 01, 2012 11:55 AM To: Diana McCaig; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: counterstain for Alcian Blue (ph2.5) If I recall correctly Newcomer Supply and Poly Scientific both sell NFR. Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 126 (All Deliveries to Suite 127) Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) wben...@cua.md From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig [dmcc...@ckha.on.ca] Sent: Wednesday, August 01, 2012 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] counterstain for Alcian Blue (ph2.5) Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: counterstain for Alcian Blue (ph2.5)
Hi Diana, I have heard of using Safranin O as a red counterstain and StatLab sells both that and Nuclear Fast Red. Thanks Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, August 01, 2012 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] counterstain for Alcian Blue (ph2.5) Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: counterstain for Alcian Blue (ph2.5)
The time and trouble of making up a solution of aluminum mordanted nuclear fast red repays you well. The staining solution contains to work well for an entire year. If you must have something else, brazalum, made by substituting brazilin for hematoxylin in Mayer's hemalum, works very well. The solution keeps its staining qualities for 2 or 3 months. Anatech sells brazalum ready-made as Brazilliant. Although Orth's lithium carmine gives beautiful results, I don't recommend it: it's a pain to make and keeps its staining power for only 2 weeks. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, FL -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, August 01, 2012 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] counterstain for Alcian Blue (ph2.5) Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Position - Manager, Workflow Consulting
Good Afternoon Histonet, We have had a new position open with a global leader in histology. Please contact me directly at m...@personifysearch.com to learn more. *Manager, Workflow Consulting* *The Company:* Our client is a leading developer and producer of innovative high-tech precision optics systems for the analysis of microstructures. As one of the market leaders in each of the fields of Cancer Diagnostics, Anatomical Pathology, Imaging Systems, Specimen Preparation and Medical Equipment. Comprising nine manufacturing facilities in seven countries, sales and service companies in 20 countries and an international network of dealers, the company is represented in over 100 countries. *The Opportunity:* The company currently has an opening for an Manager, Workflow Consulting in Clinical Diagnostics based in Chicago, IL and covers the Western Part of the US (Chicago West). The person who fills this position can live anywhere in the Western US. All applicants must not be adverse to travel, as this is a position that may require you to travel when necessary. Base: Commensurate with Experience *Primary Responsibilities:* The primary responsibility of this role will be to achieve Company sales and profitability goals by offering a value-added service to end-user that provides workflow consulting services and lean principles to optimize customer laboratories performance. Drive change in anatomic pathology laboratories utilizing Lean principles, information management, and hardware/software solutions. The solution for such a change, efficiency gains and waste elimination is the Company product offering. Additional Responsibilities: - Achieve monthly, quarterly, annual unit and revenue goals for the Division. Track KPI to measure revenue generated through Lean Consulting Services - Analyze new and existing customer laboratory organizations and workflow practices and recommend short and long-term improvements - Utilize Company Business System tools to credibly recommend changes to lab practices to eliminate waste, reduce cost and improve quality and turnaround times - Analyze and report market trends and innovative competitive activities for Lean Services - Working in conjunction with local sales representatives, Area Sales Managers and Directors of Sales plan and schedule face-face account calls to current and potential end-users. Train Sales force on basic lean principles for them to help market lean services and be able to follow-up with customers - In conjunction with Sales and Marketing, identify and develop key accounts in the territory. In conjunction with Director of Corporate Accounts, manage national accounts within territory requiring corporate coordination to enable closure and compliance of contracts - Prepare monthly territory status reports on lean activities to Management Maintain and report monthly on Workflow opportunities and projects - Manage operating expenses within assigned budget - Maintain technical, product, applications and sales skill knowledge. Maintain current knowledge of competition and market through study of competitive marketing information, competitive literature, and field surveillance or competition - Prospect for all product opportunities. Follow-up on all sales leads with status review immediately upon receipt - Participate in sales meetings and national trade shows as appropriate and authorized - Conduct Lunch and Learns, workshops, seminars or focus groups at local technical society meetings as appropriate and authorized - Promote the Company as the pathology market leader in quality and innovation *Education and Experience Required:* - BA/BS in Life Sciences or equivalent required - MBA preferred but not required - 2-5 years Histology/Pathology laboratory experience in clinical , research or industrial setting desirable but not required - Understanding of pathology marketplace with strong technical acumen - 2-5 years knowledge of Company Business Systems, Six Sigma, or Lean Principles required selling experience or consumables - Outstanding problem solving skills. Can manage multiple layers of personnel within customer site 1-3 years Histology laboratory experience in clinical, research or industrial setting desirable but not required - 1-3 years of product management or sales experience in a related discipline preferred but not required Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cost analysis
Hello all, I've been asked to update some dated test cost information. I need to come up with a a total cost (materials, burden [common materials] and labor) for IHC, ISH, special stains, and routine HE (to include processing, embedding, cutting, staining). I'm not too concerned with IHC and special stains, but I'm hoping to get some input regarding routine HE and associated work because there's a lot of factors to consider and many are variable. Has anyone done this, and would mind providing some thoughts on this process? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cost analysis
QI Macros software or Palisades Decision Tools software may help ( maybe latter for cost-seeking emphasis). It will help you track the data, make good comparision charts,find hidden waste and cost, and perform calculations with sensitivity analysis beyond Excel Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Wed, 1 Aug 2012 14:46:27 -0400 From: mjdess...@commonwealthhealth.net To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost analysis Hello all, I've been asked to update some dated test cost information. I need to come up with a a total cost (materials, burden [common materials] and labor) for IHC, ISH, special stains, and routine HE (to include processing, embedding, cutting, staining). I'm not too concerned with IHC and special stains, but I'm hoping to get some input regarding routine HE and associated work because there's a lot of factors to consider and many are variable. Has anyone done this, and would mind providing some thoughts on this process? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ENFD Testing
Happy Wednesday Histonetters I have had a request from some of our clients to do epidermal nerve fiber density testing. I have found information in how to obtain the specimen but have not found the protocol for processing, cutting, and staining the specimen. To anyone out there who is performing this testing, I would appreciate your insight. I am starting this from scratch. Any information would be welcome. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 716-250-9235 etx. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] negative controls
just wondering if we can start leaving the negative controls off of our immuno stains now as long as our procedure says we can stain without them? we use ventanas multimer detection systems. thanks so much, anita dudley providence hospital mobile, alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ACTH Antibody
Hi I am working with the DAKO ACTH clone 02A3 (concentrate) and I am getting a lot of background staining. I have tried to decreasing the incubation time of the primary and I have tried to increase the dilution however I am starting to loose signal and I still have the background and non-specific staining. I was wondering if anyone has had this experience and whether or not anyone is happy with and/or using the Cellmarque polyclonal ACTH? Thanks :-) This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destiné à la personne ou aux personnes à qui il est adressé. Il peut contenir des informations privilégiées ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autorisée est strictement défendue. Si vous n'êtes pas le destinataire de ce message, veuillez en informer l'expéditeur immédiatement et lui remettre l'original. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] negative controls
I asked my VMS rep that question and she forwarded it to the higher ups as they had not heard about this yet. Waiting for an answer but even if VMS says we can omit them, our negative aren't all that clean always so don't know if we will or not. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Wednesday, August 01, 2012 4:13 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls just wondering if we can start leaving the negative controls off of our immuno stains now as long as our procedure says we can stain without them? we use ventanas multimer detection systems. thanks so much, anita dudley providence hospital mobile, alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Outstanding Opportunity for a Pathologists' Assistant
OUTSTANDING OPPORTUNITY FOR A PATHOLOGISTS' ASSISTANT ProPath, a high volume, privately owned pathology practice located in Dallas, Texas, is seeking a Pathologists' Assistant. In this position you will be responsible for accurate description of specimens, correct dissection for microscopic diagnosis and dictation of findings for pathologist's review. The ideal candidate will have a minimum of 3 years' experience. Prefer a degree from a NACCL-accredited Pathologist's Assistant program. AAPA Fellowship/ASCP also preferred. Applicants without a degree must be able to provide documentation showing eligibility under CLIA requirements for high complexity testing. The hours for this position are 6pm to 2:30am Monday - Friday. Benefits include Medical, Dental, Short and Long Term Disability Insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! EOE For consideration send resume to: ProPath, Human Resources 1355 River Bend Drive Dallas, TX 75247 www.propath.com. Accessibility Accommodations If you require an accommodation to navigate our careers site, please send your request to accessibil...@propath.com __ Norm Burnham, MBA, MT(ASCP) Director, Anatomic Laboratory Operations ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 norm.burn...@propath.com mailto:norm.burn...@propath.com 214.237.1602 Office 214.237.1802 Fax 214.709.7127 Cell To learn more about ProPath, please visit www.propath.com http://www.propath.com/ This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: counterstain for Alcian Blue (ph2.5)
Try a weak Harris's Haematoxylin stain (half the normal stain time in your HE). The two blues are easy to distinguish. Harris's is preferred to Mayer's since Harris's seems to have a dark purple hue compared to the sky blue of Mayer's. You could even use a 1% neutral red in 1% acetic acid or even just a light eosin counterstain. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Thursday, 2 August 2012 2:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] counterstain for Alcian Blue (ph2.5) Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] calcium buildup
Hi, We have the same instrument. I love the thing to death! The water reservoirs can be removed by pulling them out like the other ones. The water input remains in place while the rubber gasket slides off. By the way, do this with the instrument off so it doesn't spill all over the place. Wash out the reservoir well then you can remove the water input by holding the large stainless steel nut at the back and twisting counter clockwise. If the calcium buildup is bad some channel lock pliers may come in handy. Don't loose the rubber gasket that is behind the stainless steel nut. The majority of the crud will come of with a scrub in detergent, but if it is really bad a soak in dilute HCl should help. You could leave it soaking overnight in hot water too. This would also be a good time to remove the stainless steel trays the reservoirs set on and clean out the drain. Fungal growths can build up there and can actually clog the drain if left unchecked. Actually this should all be part of a periodic maintenance plan. We have a service take care of it for us (Belair in New Jersey comes out for us) and I also take it down and clean it out before our Christmas break. Amos Brooks On Wed, Aug 1, 2012 at 11:25 AM, histonet-requ...@lists.utsouthwestern.eduwrote: Message: 19 Date: Wed, 1 Aug 2012 10:57:16 + From: Molinari, Betsy bmolin...@texasheart.org Subject: [Histonet] calcium buildup To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: fb4aad97a0b1e0439051cda66d19edde24bd0...@svr-exch03.thi2.com Content-Type: text/plain; charset=utf-8 Hi, Does anyone know of a way to remove calcium buildup on the wash nozzles in the water reservoirs. I have the Tissue-Tek DRS 2000. Thank you. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston,TX 77030 832-355-6524 832-355-6812 (fax) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] counterstain for Alcian Blue (ph2.5)
Hi, We use Neutral Red in 1% solution. (Not being in the lab right now I think it is made up in 1% acetic acid, but I could be wrong.) It is much faster than NFR (nucear fast red). It is about one minute to stain as opposed to the 5 to 15 if the NFR solution is old. It is easy as heck to make up and neutral red has a much longer shelf life too. The only difference I have noted is that the stain is a slightly darker (rusty) red than the *really* light pink of NFR. Amos On Wed, Aug 1, 2012 at 1:00 PM, histonet-requ...@lists.utsouthwestern.eduwrote: Message: 4 Date: Wed, 1 Aug 2012 12:50:14 -0400 From: Diana McCaig dmcc...@ckha.on.ca Subject: [Histonet] counterstain for Alcian Blue (ph2.5) To: histonet@lists.utsouthwestern.edu Message-ID: dcfd9e6a390e294aaf3a2561cd32e5c417a90...@ckhamail1.ckha.on.ca Content-Type: text/plain; charset=us-ascii Can you please let me know what options for counterstain there are other than Nuclear Fast Red or a supplier who sells the prepared solution Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
