[Histonet] HT Position in Colorado
Great part time opportunities' for Histotechnician's in Centennial, Colorado! Colorado GI Pathology is looking for certified HT's or HTL's to join their existing laboratory staff. Candidate must meet the following criteria: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * HT ASCP Certified Duties include: * Grossing * Embedding * Microtomy * Staining * Ability to be flexible and take on additional duties' as needed This is a part time that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mh...@miracals.commailto:mh...@miracals.com Meredith Hale HT (ASCP)cm Operations Liaision Director and Education Coordinator Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mh...@miracals.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] histomorphometrics done for bone samples
Hello, I have a Dr. who is asking about how to get histomorphometrics done for bone samples, data needed is % of new bone present in samples from sites grafted with freeze-dried mineralized cancellous allograft bone. We can do the plastics on it but the computer assisted part, I do not know anything about. Thanks Hans B Snyder Histologistics 100 Barber ave Worcester, MA 01606 www.histologistics.com h...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] histomorphometrics done for bone samples
Hello Hans! If you would like to discuss, please feel free to call me at your convenience. Best Regards, Jack Jack Ratliff Ratliff Histology Consultants, LLC Chairman, Hard Tissue Committee - National Society for Histotechnology On Aug 30, 2012, at 8:37 AM, Hans B Snyder h...@histologistics.com wrote: Hello, I have a Dr. who is asking about how to get histomorphometrics done for bone samples, data needed is % of new bone present in samples from sites grafted with freeze-dried mineralized cancellous allograft bone. We can do the plastics on it but the computer assisted part, I do not know anything about. Thanks Hans B Snyder Histologistics 100 Barber ave Worcester, MA 01606 www.histologistics.com h...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pros and cons of Leica ST5050 slide stainer
Hello All, Can someone help me with the pros and cons of using a Leica ST5050? Specifically, are there any quirks or inconveniences in using it? Thanks! Kevin Seguin kevinseg...@gmi-inc.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fragmented Biopsy Samples
Any hints out in Histoland on how to eliminate thin needle biopsy samples from fragmenting during handling from gross through embedding. Don't want to use sponges. Thanks, Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.wil...@baylorhealth.edu ** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Fragmented Biopsy Samples
Donna, try the new SecureSette from ThermoFisher. They enclose the sample at grossing and do not require manipulation at embedding - just add wax and cool. Very interesting product. We tried them and they work well for some samples. However, they only accommodate straight needle bx of 2cm or less. Our prostate cores were 2.5 cm at times so it didn't quite fit. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.mor...@ucsfmedctr.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Thursday, August 30, 2012 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fragmented Biopsy Samples Any hints out in Histoland on how to eliminate thin needle biopsy samples from fragmenting during handling from gross through embedding. Don't want to use sponges. Thanks, Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.wil...@baylorhealth.edu ** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Fragmented Biopsy Samples
Try using histoscreen cassettes and that will eliminate the need for wrapping the tissue. If still insist on wrapping, ensure that they are dipping the tissue paper in formalin prior to putting the tissue on the paper. Also at embedding, if the tissue is stuck to the paper, take a blade and put it against the tissue and gently push to loosen the tissue, repeat this the length of the tissue. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Thursday, August 30, 2012 10:41 AM To: Willis, Donna G.; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Fragmented Biopsy Samples Donna, try the new SecureSette from ThermoFisher. They enclose the sample at grossing and do not require manipulation at embedding - just add wax and cool. Very interesting product. We tried them and they work well for some samples. However, they only accommodate straight needle bx of 2cm or less. Our prostate cores were 2.5 cm at times so it didn't quite fit. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.mor...@ucsfmedctr.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Thursday, August 30, 2012 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fragmented Biopsy Samples Any hints out in Histoland on how to eliminate thin needle biopsy samples from fragmenting during handling from gross through embedding. Don't want to use sponges. Thanks, Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.wil...@baylorhealth.edu ** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DAPI staining
Someone sent me a question regarding DAPI staining ( I generally use Hoechst )...but anyway, they are having smearing of nuclear contents. I would guess that is from rupturing of the nuclear membraneover fixation? Over air drying? They are fixing 10 mins in cold acetone and 10 mins to air dry. What do the experts out there say? This could be a learning moment for me , as well. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AUTO: Gina Rodriguez is out of the office. (returning 09/05/2012)
I am out of the office until 09/05/2012. I will respond to your message when I return. If you need immediate assistance please contact 800-248-0123 or tech.supp...@leica-microsystems.com Note: This is an automated response to your message Histonet Digest, Vol 105, Issue 35 sent on 8/30/2012 12:03:22 PM. This is the only notification you will receive while this person is away. _ This e-mail has been scanned for viruses by Verizon Business Internet Managed Scanning Services - powered by MessageLabs. For further information visit http://www.verizonbusiness.com/uk ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DAPI staining
Hi Carol, Does this person put them in a freezer for 20 mins or so after DAPI staining? Someone once told me this helps with smearing. I'm not really sure if its true, but couldn't hurt to try it. Mark On Thursday, August 30, 2012, Barone, Carol wrote: Someone sent me a question regarding DAPI staining ( I generally use Hoechst )...but anyway, they are having smearing of nuclear contents. I would guess that is from rupturing of the nuclear membraneover fixation? Over air drying? They are fixing 10 mins in cold acetone and 10 mins to air dry. What do the experts out there say? This could be a learning moment for me , as well. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
