RE: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray
Ice is still the best coolant because it adds moisture. As Lee Luna used to say Rehydrate, rehydrate, rehydrate For tough blocks, face it then add soapy water to ice with some ammonium hydroxide. Like magic! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Monday, October 01, 2012 2:01 PM To: joelle weaver; histotech...@gmail.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray I was once worked at a place that had this sign on the wall that everyone saw as you entered the training room. When you think you are through learning, you are through here Blows my mind when I run into people that refuse to learn anything current. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Saturday, September 29, 2012 7:12 PM To: histotech...@gmail.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray JennyYou don't need to respond to this, but I will post in case there is anyone else out there who is going through the same experiences and feeling discouraged. There are many people in the field like this. I have been out there at least a little while and I went through the same response once out of histology school and get attitude all the time, still to this day, even though I have jumped through all the usual hoops at this point. I can tell you that while working in some labs, I thought of quitting histology almost weekly because I got so sick of this kind of thing People have gotten into the field in various ways, and they sometimes get into ruts and they don't get out there much to learn all the new techniques and information. People coming in with ideas threaten the status quo, and sometimes it is just difficult many people to change. I haven't quit histology yet though, and you shouldn't let other people drive you out or make you doubt yourself either. Trust me, we need educated and trained people in this field in a desperate way. Look, we all have stuff to learn, new and old! If you stop learning and believe you know it all, you are a real drag to everyone and holding back others who want to learn and grow. If you are a newer tech, I think that can be a plus. You are fresh and full of enthusiasm and new ideas. The seasoned can share what they have learned from time and experience, and you can bring new ideas and your fresh enthusiasm and energy- I don't see why that can't be a win-win. Anyhow, all of this are just my opinions, and I may get slammed for these comments like I have many times before, but as far as I can tell from what you have posted, you are not wrong, and your techniques seem reasonable for the hospital clinical setting. In addition, you seem to understand why they work for you and the technical rationale behind them. To me this is good. Many people have been taught how to do things, but not why. We need more people who want to know why and who care about quality. Please read the other posts about blades ( they said it best already) but I feel that is crap about skimping on them ( sorry) . No patient is worth less than any disposable blade. That is why they call them disposable. You should not waste supplies, that is irresponsible, but within reason you need decent supplies to get the best quality you can possible can. Ice is cheap, much cheaper than freeze it spray..(as someone mentioned and a good point) .. so there is no valid economic/operational reason there that I can see to justify any of that. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Sat, 29 Sep 2012 18:26:38 -0400 Subject: Re: [Histonet] Cooling paraffin blocks with ice VS. Freezing Spray From: histotech...@gmail.com To: joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu Thanks everybody for your answers. I cant respond them all but I concluded that the best way to get good sections is too chill the blocks on ice because I agree that it facilitates the process. I really don't understand why my supervisor depends so much on freezing sprays to cut and the pathologist has never complained about artifacts caused by them but I do believe that they are present because I have seen them getting formed. It makes sectioning difficult because you try to get sections free of holes and that contributes to the problem. At my lab is the same thing. My supervisor is in charge of the embedding and she just use the ice only for hardening the paraffin block. We don't have a standard embedding center with cold plate. Since is a small lab we just have a heating plate where we handle the
RE: [Histonet] Ventana temp veriifer slides
We have been running in every position every quarter!! Very expensive. We record the lot # of verifier slides and the results. If one position fails, we repeat. No photos. We recently took advantage of Ventanas special promotion. They will come here and have someone perform all quarterly maintenance...temp slides and decon. Ended up being HALF the cost -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Monday, October 01, 2012 1:42 PM To: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana temp veriifer slides I'm doing the even one quarter and the odds the next also. I number the slides in Sharpie to cprrespond to the heat pad # after they are finished and take a photo of them for documentation, then toss the slides. es...I've decided to do the even's 1 quarter and the odds the next quarter (because of cost)...do you just run them, document it and throw them away? Or does anyone make a copy of them and save in the QC book? Thanks! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] duel stains
No we are still doing the triple stain, just stopped billing three. The pathologist decided that the patient specimen was more valuable. Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of llhartma...@comcast.net [llhartma...@comcast.net] Sent: Monday, October 01, 2012 7:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] duel stains Hi Netters, Just curioushas anyone stopped doing dual IHC (ie. PIN4) du e to the charging changes? We are going to start doing the three stains separately. Any opiniions? Linda Hartman HT(ASCP) MNMC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MIXED fiber type identification by Immunohistochemistry
Greetings to all, In immunohistochemistry, I am encountering confusion in designating muscle fibers (based on staining color intensity) to a particular fiber type. This problem is particularly bothersome when i encounter the fibers stained with 2 different colors, one color has good intensity while other seems to be of light intensity. I don't have any criterion whether to consider this light staining as actual staining, which would classify the fibers as MIXED fibers or should i consider this light staining as background/non specific staining in which case the fibers will be classified as PURE fibers. Does anybody can suggest any criterion/tips they have been using to identify muscle fiber type as correctly as possible? I can understand that identifying MIXED fibers is subjective in immunohistochemistry, but it would be of immense help if i could get to know of any objective suggestions. Animal= spinal cord injured Rat Muscle = Soleus Section Thickness = 10µm I will really appreciate the help! Thanks, hope to hear from ppl soon! BEST, AR ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] duel stains
We've always just billed for one stain so no difference for us; we'll continue doing it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of llhartma...@comcast.net Sent: Monday, October 01, 2012 6:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] duel stains Hi Netters, Just curioushas anyone stopped doing dual IHC (ie. PIN4) du e to the charging changes? We are going to start doing the three stains separately. Any opiniions? Linda Hartman HT(ASCP) MNMC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Acid Phosphatase Reaction
Acid phosphatase is a standard procedure in FFPE (Formalin Fixed Paraffin Embedded) tissues. You will need to process your tissues accordingly. Xylene is also standard procedure even when it should be avoided because of toxicity. There are many other alternatives to xylene usage. I advise you to check in some TEM text that can answer your doubts. René J. From: Duraine, Lita dura...@bcm.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Monday, October 1, 2012 6:35 PM Subject: [Histonet] Acid Phosphatase Reaction Hello to all Histologists, Perhaps someone can help me. At our institution we primarily use electron microscopy for slides with toluidine blue, and grids for TEM. The students here also do some immunostaining on their tissues. We have a student though who would like to try using the Acid Phosphatase Reaction to identify lysosomes as another alternative data to add to his paper. Not having done any type of paraffin sectioning before how would we proceed? I understand the basic tissue processing recipe with formalin, however does the procedure call for a special machine? I noticed that all the recipes I have looked at online refer to using cassettes. We primarily use microwave and vacuum to process our tissue in Karnovsky's for TEM. Would the xylene cause any problems for us? Are there any other stains that could work on resin embedded sections besides toluidine blue that would specifically stain lysosomes? You advice would be greatly appreciated. Thank you, Lita Duraine EM Technologist Bellen Lab HHMI- Molecular Genetics Duncan Neurological Research Institute 1250 Moursund St. Houston, TX 77030 Rm: N1165.17 MS: N1125.50 832-824-8772 TEM Room 979-549-6526 Cell http://flypush.imgen.bcm.tmc.edu/lab/people/lita.php ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Reliable Histology Team Member to embed and cut for you.
I am not the only forum member who is concerned about this. I have received private messages from others who agree with me and choose not to reply to all. I will not repost them out of respect for their privacy. The facts that you take what you do seriously, or are not disrespectful are moot. The fact that does apply here is that advertising is not allowed on Histonet. The staffing agencies that* occasionally* post on here are offering a list of open jobs. You are soliciting for your services, three times in one week. (9/25, 9/26, 10/1) Histonet is not a forum for marketing, pitching, plugging, promulgating or selling. I hope it stays that way. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cold room alternative
The building I am in does not have a cold room and I would like to incubate some samples overnight on an orbital shaker at 4°C. Can anyone recommend a refrigerator with an interior electrical outlet or another alternative? Thanks. Aaron Aaron M. Johnson Assistant Professor Department of Speech and Hearing Science University of Illinois at Urbana-Champaign ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Traveling
I'm considering becoming a traveling histotech in the future. Are there any traveling techs or any techs that traveled in the past on the forum that can offer some ups and downs positive and negatives of traveling Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cold room alternative
Aaron, We have a refrigerator from VWR that has a glass door like a Deli frig. and it has an electrical outlet inside as well as outside. That could be a starting place to look. :) Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.b...@ucdenver.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Aaron Sent: Tuesday, October 02, 2012 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cold room alternative The building I am in does not have a cold room and I would like to incubate some samples overnight on an orbital shaker at 4°C. Can anyone recommend a refrigerator with an interior electrical outlet or another alternative? Thanks. Aaron Aaron M. Johnson Assistant Professor Department of Speech and Hearing Science University of Illinois at Urbana-Champaign ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re:
http://ifet.com.ar/www.itrustyou.foxnews.realwork.php ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cold room alternative
Ditto here, ours are manufactured by True, but you could probably find one cheaper through one of the used lab equipment vendors on the web. Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bell, Pat Sent: Tuesday, October 02, 2012 6:13 PM To: 'Johnson, Aaron'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cold room alternative Aaron, We have a refrigerator from VWR that has a glass door like a Deli frig. and it has an electrical outlet inside as well as outside. That could be a starting place to look. :) Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. RC1-S, L18-8402C Aurora, Co. 80045 303-724-6077 pat.b...@ucdenver.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Aaron Sent: Tuesday, October 02, 2012 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cold room alternative The building I am in does not have a cold room and I would like to incubate some samples overnight on an orbital shaker at 4°C. Can anyone recommend a refrigerator with an interior electrical outlet or another alternative? Thanks. Aaron Aaron M. Johnson Assistant Professor Department of Speech and Hearing Science University of Illinois at Urbana-Champaign ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Traveling
If you do that and announce your availability on Histonet, you are likely to be criticized publicly by a few, while others send email and twitters to one another privately discussing your attributes, and those who like having the information that you are available probably will not speak up in your support. But since there is no such thing as bad publicity, you will have to decide the +/- of it. On 10/3/2012 5:49 AM, Demetria Ross wrote: I'm considering becoming a traveling histotech in the future. Are there any traveling techs or any techs that traveled in the past on the forum that can offer some ups and downs positive and negatives of traveling Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Reliable Histology Team Member to embed and cut for you.
Go get em Jay! I agree with you 100% this forum should be kept free of blatant advertising. I'd also like to give you credit for not stooping to the level of this anonymous poster after he or she publicly tried to belittle you. Marc DeCarlo On Tuesday, October 2, 2012, Jay Lundgren wrote: I am not the only forum member who is concerned about this. I have received private messages from others who agree with me and choose not to reply to all. I will not repost them out of respect for their privacy. The facts that you take what you do seriously, or are not disrespectful are moot. The fact that does apply here is that advertising is not allowed on Histonet. The staffing agencies that* occasionally* post on here are offering a list of open jobs. You are soliciting for your services, three times in one week. (9/25, 9/26, 10/1) Histonet is not a forum for marketing, pitching, plugging, promulgating or selling. I hope it stays that way. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu javascript:; http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reliable Histology Team Member to embed and cut for you.
Ok, let me put this issue to bed. It's fine you feel you need to be the voice for those who have all the help they need and will never need the services of a histology professional to help cover staffing shortages or inadequate/ineffective staffing. There are thousands of subscribers to this list and I respect every single one of them. HistoCare is for those who NEED professional assistance on an interim basis. And let's be clear, I'm not just a tech looking for job once in a while. I'm a professional extending my expertise and invaluable abilities to help when the need arises on relatively short notice without compromising patient care. Your messages seem to have a very petty tone and not respectful of the nature of the work we do. It's almost as if you are not even of this profession. I care about all those people who go to doctor to have a million tests done to see what's wrong with them and have to wait days and weeks before they get any kind of news to settle their nerves or some glimmer of hope. I want to minimize that inconvenience as much as I can. I am a real person first, a histology professional second on the other side of this email just like there is a real patient on the other side of that slide. You only care about HistoCare advertising on one of the few forums histology professionals have to exchange thoughts, ideas, and RESOURCES. If the best you can add to histonet is criticism, it doesn't need you or those like you. This is a small community with very high turnover, low job satisfaction, some employed individuals with marginal or inadequate abilities, and little interest in others to want to break into this profession. Heck even the ones that's been his field for a long time express dismay, let alone the newbies who can't get the proper support from their own supervisors! Good grief! Those who rely on histonet for advice and ideas should also be able to search for dependable lab support and hope that there is a good resource for them to call upon. Please do me a favor and respectfully bow out and resist the urge to respond further as I have no interest in debating. I'd rather spend my time being productive. There isn't a policy specifically stating i can't make HistoCare available for those who may search for assistance. Lets not forget Histonet is a courtesy to us all for interests in histology and what it is not, is a vehicle to complain. I was initially open to your suggestions for alternatives and offered you the opportunity to respond with solutions acceptable to 'you' but I see you would rather be a complainer than a helper. Any future responses from you referring to this matter directly or indirectly will be considered harassment and forwarded to the appropriate parties. Sincerely HistoCare www.HistoCare.com On Oct 2, 2012, at 4:19 PM, Jay Lundgren jaylundg...@gmail.com wrote: I am not the only forum member who is concerned about this. I have received private messages from others who agree with me and choose not to reply to all. I will not repost them out of respect for their privacy. The facts that you take what you do seriously, or are not disrespectful are moot. The fact that does apply here is that advertising is not allowed on Histonet. The staffing agencies that occasionally post on here are offering a list of open jobs. You are soliciting for your services, three times in one week. (9/25, 9/26, 10/1) Histonet is not a forum for marketing, pitching, plugging, promulgating or selling. I hope it stays that way. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Reliable Histology Team Member to embed and cut for you.
Kudos to you too for reading the entire message. If you had, you'd see the other person initiated the public belittling. I am not some pushover who won't defend my professionalism when subtly attacked by some closed minded person and his cheerleaders. I didn't ask for this. It was brought to me. Do you think every message you or others send or respond to is important or relevant to every single subscriber? I'll be the first to tell you they aren't, the difference being the other subscribers practice self control and simply ignore topics which don't pertain to their particular need at the time. It takes less effort to delete messages from the list than to respond to something of no value to the subscriber. On Oct 2, 2012, at 9:26 PM, Marc DeCarlo boneima...@gmail.com wrote: Go get em Jay! I agree with you 100% this forum should be kept free of blatant advertising. I'd also like to give you credit for not stooping to the level of this anonymous poster after he or she publicly tried to belittle you. Marc DeCarlo On Tuesday, October 2, 2012, Jay Lundgren wrote: I am not the only forum member who is concerned about this. I have received private messages from others who agree with me and choose not to reply to all. I will not repost them out of respect for their privacy. The facts that you take what you do seriously, or are not disrespectful are moot. The fact that does apply here is that advertising is not allowed on Histonet. The staffing agencies that* occasionally* post on here are offering a list of open jobs. You are soliciting for your services, three times in one week. (9/25, 9/26, 10/1) Histonet is not a forum for marketing, pitching, plugging, promulgating or selling. I hope it stays that way. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
