[Histonet] Re: Stain for HP
Staining gastric biopsy specimens for Helicobacter pylori: practice varies greatly in various labs I've worked in. One lab does a blue stain (generic equivalent of Diff-Quik II), while the other does immunohistochemistry, each of them on all specimens. I've also seen labs that did a full tissue Giemsa, and one that did a cresyl echt violet stain. Haven't seen a routine Warthin-Starry in many years. I think everyone bills every one of these stains they do. There are still a few hold-outs for real men can see 'em on the H E. As far as I know, no studies have been done comparing the sensitivity (we'll forget specificity) of the various methods. My personal opinion is that none of the dye or silver methods is conspicuously better than the others. Reading the blue stain is time-consuming; I use oil immersion magnification (which real men disdain) to confirm all positives (and confirm Helicobacter heilmannii, which I have seen once), and on all apparent negatives with acute inflammation. The IHC can be read rapidly with much less magnification. I note that many pathologists sign out any bacteria they see in a blue stain as Helicobacter, including the frequent bugs that get carried down from the oral cavity as the scope advances. These pathologists should definitely switch to IHC. So if I have one gastric biopsy case a day, I'm happy with the blue stain. If I have ten, I want IHC. If there is no inflammation, I can do without any stain. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Stain for HP
Also Alcian Yellow -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Friday, October 19, 2012 5:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Stain for HP Staining gastric biopsy specimens for Helicobacter pylori: practice varies greatly in various labs I've worked in. One lab does a blue stain (generic equivalent of Diff-Quik II), while the other does immunohistochemistry, each of them on all specimens. I've also seen labs that did a full tissue Giemsa, and one that did a cresyl echt violet stain. Haven't seen a routine Warthin-Starry in many years. I think everyone bills every one of these stains they do. There are still a few hold-outs for real men can see 'em on the H E. As far as I know, no studies have been done comparing the sensitivity (we'll forget specificity) of the various methods. My personal opinion is that none of the dye or silver methods is conspicuously better than the others. Reading the blue stain is time-consuming; I use oil immersion magnification (which real men disdain) to confirm all positives (and confirm Helicobacter heilmannii, which I have seen once), and on all apparent negatives with acute inflammation. The IHC can be read rapidly with much less magnification. I note that many pathologists sign out any bacteria they see in a blue stain as Helicobacter, including the frequent bugs that get carried down from the oral cavity as the scope advances. These pathologists should definitely switch to IHC. So if I have one gastric biopsy case a day, I'm happy with the blue stain. If I have ten, I want IHC. If there is no inflammation, I can do without any stain. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: labeling pre-made slides
Thank you for all the suggestions, I now have a few ideas of how to correct this. -Original Message- From: Goins, Tresa [mailto:tgo...@mt.gov] Sent: Thursday, October 18, 2012 4:27 PM To: Hutton, Allison; histonet@lists.utsouthwestern.edu Subject: RE: labeling pre-made slides I don't know if you tried these markers specifically, but we have seen variation in quality. We changed vendors because we began having problems with our original source - the vendor changed their supplier and tried selling the pen as the same as - it was not even close. Also, you may try cleaning the frosted area with acetone before labeling. Tresa -Original Message- From: Hutton, Allison [mailto:ahut...@dh.org] Sent: Thursday, October 18, 2012 1:39 PM To: Goins, Tresa; histonet@lists.utsouthwestern.edu Subject: RE: labeling pre-made slides Some further clarification... *We manually coverslip *We have tried the markers upon reciept but have trouble with them staying on due to the spray fixative ending up on the frosted end as well. -Original Message- From: Goins, Tresa [mailto:tgo...@mt.gov] Sent: Thursday, October 18, 2012 3:08 PM To: Hutton, Allison; histonet@lists.utsouthwestern.edu Subject: RE: labeling pre-made slides We have no label maker - so the slides are labeled with a Securline Marker II from EMS - stays put through all treatments. Prior to delivery, a paper label is printed and affixed to the slide over the hand-written label. Tresa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Thursday, October 18, 2012 12:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] labeling pre-made slides We use the Leica IPS to label our cytology slides, however, we do get quite a few slides from doctors who make smears and spray fix them before they come to us. Unfortunately, they use pencil to label and this usually wipes off during staining and coverslipping. Does anyone else receives slides like this and then how are they permanently labeled? We would like to avoid going back to paper labels if at all possible. Any advice or suggestion is appreciated Thanks Allison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Histology Careers Bulletin 10/19/2012 TGIF and a beautiful Fall weekend to look forward to!
Hi Histonetters!! TGIF How are you? Are you enjoying the sights, sounds and flavors of Fall? I know I am enjoying college football after all I am a southern gal and you know how we southerners are about our Football Saturdays in the South. On the other hand I am still patiently waiting for that real Fall stuff. You know like crisp cool air, beautiful fall colors, crunching leaves and steaming bowls of soup. What are you enjoying most of all about this season? I also wanted to tell you about some new job opportunities and a new feature in my career bulletin. The new feature is: The Spotlight Job I will be spotlighting a particular position and providing details in the email. To qualify for the spotlight the position has to be beyond the scope of a regular histology job so you might not see one every time. These are the one in a million opportunities!! Todays SpotlightJob: Lead Histology Tech/Supervisor Growing State of the Art Lab in Topeka, KS My client is a growing state of the art pathology lab located in Topeka KS. They are looking for someone with at least 2-3 years of histology experience and some supervisory experience as well. ASCP HT or HTL is required. You will have the opportunity to work with all types of tissue, grossing and ihc (if you need training it will be provided), You will also have the opportunity to learn and use digital pathology. My client offers a great salary 100% paid benefits for employees, relocation assistance and a bonus program. I have a number of other really great opportunities For ASCP certified or eligible histologists: Charlotte, NC Hospital based all shifts available HT/HTL required Charlotte, NC Private Lab Grossing Histotech Afternoon shift HT/HTL CLIA qual to gross Nashville, TN Private Path Lab Afternoon shift ASCP HT/HTL or eligible Collegeville, PA New Lab Day shift HT/HTL and CLIA qual to gross Reading, PA In Office Path Lab - Dermpath Histotech Day Shift HT/HTL and CLIA qual to gross Dallas, TX Private Reference Lab IHC Tech ASCP HT/HTL, QIHC preferred. Portland, ME Private Reference Lab Histotech ASCP HT/HTL or eligible Topeka, KS Lead Histotech/Supervisor see spotlight job for details. If you are interested in participating in my referral program in addition to histotechs for these positions I also need cytotechs in TN and SD. If you or anyone you know might be interested in any of these opportunities or would like help with a job search in another area of the USA please contact me. I can be reached at rel...@earthlink.net or toll free at 866-607-3542. Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse Model
From: Amy Porter port...@msu.edu To: 'Histonet' histonet@lists.utsouthwestern.edu Sent: Thursday, October 18, 2012 12:35 PM Subject: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse Model Anyone out there have CD31 working well on FFPE samples for Mouse Samples?? I know this might be a long shot, however I haven't looked for anything on this in quite awhile. Amy S. Porter, HT(ASCP) QIHC Michigan State University Investigative HistoPathology Laboratory William S. Spielman, Ph.D. - Director Patricia K. Senagore, M.D. - Consulting Pathologist Department of Physiology / Human Pathology Biomedical Physical Sciences Building 567 Wilson Road - Room 2133 East Lansing, MI 48824-3320 Phone: 517-884-5026 Fax: 517-432-1368 port...@msu.edu http://www.humanpathology.msu.edu/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet I use The antibody from Biocare Medical, and I also purchase the Kit that is designed for this antibody. Works well, even on bone. Nancy Lowen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD31 for FFPE IHC on Mouse
Amy Can you please share the responses you got with the rest of us Thanks Mark Message: 8 Date: Thu, 18 Oct 2012 16:21:55 -0400 From: Amy Porter port...@msu.edu Subject: RE: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse Model To: 'Amy Porter' port...@msu.edu,'Histonet' histonet@lists.utsouthwestern.edu Message-ID: 003101cdad6e$3792fc80$a6b8f580$@edu Content-Type: text/plain;charset=US-ASCII Thanks to all for responses..looks like most roads lead to once place which is spectacular!! Just what I needed. Amy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Porter Sent: Thursday, October 18, 2012 3:36 PM To: 'Histonet' Subject: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse Model Anyone out there have CD31 working well on FFPE samples for Mouse Samples?? I know this might be a long shot, however I haven't looked for anything on this in quite awhile. Amy S. Porter, HT(ASCP) QIHC Michigan State University Investigative HistoPathology Laboratory William S. Spielman, Ph.D. - Director Patricia K. Senagore, M.D. - Consulting Pathologist Department of Physiology / Human Pathology Biomedical Physical Sciences Building 567 Wilson Road - Room 2133 East Lansing, MI 48824-3320 Phone: 517-884-5026 Fax: 517-432-1368 port...@msu.edu www.humanpathology.msu.edu ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: processing after Bouin's fixation
Sending this for a friend, please do not reply to me. _ From: Burton, Lynn [mailto:lynn.bur...@illinois.gov] Sent: Thursday, October 18, 2012 8:43 AM To: raest...@grics.net Subject: FW: processing after Bouin's fixation Rae, I have been trying to get this out on Histonet and I can't seem to address it correctly. It is for Dr. Dybas. Would you please help me? Lynn From: Burton, Lynn Sent: Wednesday, October 17, 2012 3:14 PM To: 'histo...@lists.utsouthwester.edu'; 'histonet-boun...@lists.utsouthwestern.edu' Subject: FW: processing after Bouin's fixation From: Burton, Lynn Sent: Wednesday, October 17, 2012 1:46 PM To: 'on behalf of Lynn Burton' Subject: processing after Bouin's fixation Hi, I have a question about the step in between Bouin's fixation and alcohol dehydration of rat brain tissue, nucleus accumbans. Several procedures are suggested by various resources, what are any of you using successfully? Lynn Burton Animal Disease Lab Galesburg,Il ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
