RE: [Histonet] Charges for cutting research case

2012-11-08 Thread Dessoye, Michael J
We have worked out a cost for cutting and staining HE's, so we
basically take that cost and mark it up.  

Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General
Hospital | An Affiliate of Commonwealth Health |
mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre,
PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 
 

-Original Message-
From: Cheri Miller [mailto:cmil...@physlab.com] 
Sent: Wednesday, November 07, 2012 12:44 PM
To: histonet-boun...@lists.utsouthwestern.edu
Cc: histonet
Subject: [Histonet] Charges for cutting research case

I would like some direction in regards to how to charge a client for
cutting multiple slides and levels  anywhere between 10 to 50 slides per
block of a Technical component case. These would be research cases. Any
help directing me would be greatly appreciated. Thank, Cheri

Cheryl A. Miller HT(ASCP)cm
Histology Supervisor,
Hygiene Officer
Physicians Laboratory Services
4840 F Street
Omaha, NE. 68127-0999
402 731 4145 ext. 554




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[Histonet] Re: [Histology] Congo Red Stain

2012-11-08 Thread Sherwood, Margaret
To all:

Would people please share their method for doing Congo Red staining?  (We are 
not interested in making up the reagents from powder form).

Thanks!
Peggy


Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherw...@partners.org



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RE: [Histonet] Re: [Histology] Congo Red Stain

2012-11-08 Thread Amy Porter
Sigma Aldrich makes a very nice kit for this. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood,
Margaret
Sent: Thursday, November 08, 2012 10:01 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Re: [Histology] Congo Red Stain

To all:

Would people please share their method for doing Congo Red staining?  (We
are not interested in making up the reagents from powder form).

Thanks!
Peggy


Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherw...@partners.org



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the
e-mail contains patient information, please contact the Partners Compliance
HelpLine at http://www.partners.org/complianceline . If the e-mail was sent
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sender and properly dispose of the e-mail.


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[Histonet] RE: Re: [Histology] Congo Red Stain

2012-11-08 Thread Laurie Colbert
I've tried kits from both Richard Allan/Thermo and American Master Tech.  Both 
staining methods are similar and very easy to perform - 20 minutes in Congo Red 
and counterstain in Hematoxylin.

Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, 
Margaret
Sent: Thursday, November 08, 2012 7:01 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Re: [Histology] Congo Red Stain

To all:

Would people please share their method for doing Congo Red staining?  (We are 
not interested in making up the reagents from powder form).

Thanks!
Peggy


Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherw...@partners.org



The information in this e-mail is intended only for the person to whom it is 
addressed. If you believe this e-mail was sent to you in error and the e-mail 
contains patient information, please contact the Partners Compliance HelpLine 
at http://www.partners.org/complianceline . If the e-mail was sent to you in 
error but does not contain patient information, please contact the sender and 
properly dispose of the e-mail.


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[Histonet] Manual IHC for Melan-A

2012-11-08 Thread Michelle M. Mackinnon
Has anyone done manual IHC for Melan-A?  If yes, who help set up the manual 
protocol?
Thanks,
Michelle

Michelle M MacKinnon HT, (ASCP)
Histology Supervisor
Dermatology Consultants. PA
1215 Town Centre Drive, Suite 200
Eagan, MN 55123
Phone: 651-255-3462
Fax: 651-255-3461

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AW: [Histonet] Re: [Histology] Congo Red Stain

2012-11-08 Thread Gudrun Lang
We use the Merck kit but have turned the order of reagenses: first congored
and differentiation, then hemalaun. That masks much of unwanted staining.
Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Sherwood,
Margaret
Gesendet: Donnerstag, 08. November 2012 16:01
An: 'histonet@lists.utsouthwestern.edu'
Betreff: [Histonet] Re: [Histology] Congo Red Stain

To all:

Would people please share their method for doing Congo Red staining?  (We
are not interested in making up the reagents from powder form).

Thanks!
Peggy


Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherw...@partners.org



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the
e-mail contains patient information, please contact the Partners Compliance
HelpLine at http://www.partners.org/complianceline . If the e-mail was sent
to you in error but does not contain patient information, please contact the
sender and properly dispose of the e-mail.


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[Histonet] RE: Manual IHC for Melan-A

2012-11-08 Thread Goins, Tresa

We do manual staining for everything - here is the abbreviated version for 
Melan A -

Thermo Scientific MS799
Clone A103; Mouse MAb Isotype IgG1

Protocol:

1.  Dewax and hydrate sections
2.  Retrieve with HIER - EDTA pH 8 20 min  
3.  Block with NGS for 15 min
4.  Treat with primary Ab for 30 min
5.  Apply Link #1 - biotinylated α-mouse
6.  Apply Link #2 - streptavidin enzyme complex
7.  Rinse with water and apply chromogen 
8.  Rinse with dH2O 
9.  Counterstain, dry and coverslip

Results:
Staining is cytoplasmic
Granular cytoplasmic staining of adrenal cortical cells; minimal or no 
staining of kidney
Equivalent results using canine and feline tissue during validation

Tresa



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michelle M. 
Mackinnon
Sent: Thursday, November 08, 2012 9:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Manual IHC for Melan-A

Has anyone done manual IHC for Melan-A?  If yes, who help set up the manual 
protocol?
Thanks,
Michelle

Michelle M MacKinnon HT, (ASCP)
Histology Supervisor
Dermatology Consultants. PA
1215 Town Centre Drive, Suite 200
Eagan, MN 55123
Phone: 651-255-3462
Fax: 651-255-3461

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Re: [Histonet] Manual IHC for Melan-A

2012-11-08 Thread Rene J Buesa
If you use an autostainer, just repeat all the steps and incubation times, but 
manually.
René J.




From: Michelle M. Mackinnon mmackin...@dermatologyconsultants.com
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Thursday, November 8, 2012 10:59 AM
Subject: [Histonet] Manual IHC for Melan-A

Has anyone done manual IHC for Melan-A?  If yes, who help set up the manual 
protocol?
Thanks,
Michelle

Michelle M MacKinnon HT, (ASCP)
Histology Supervisor
Dermatology Consultants. PA
1215 Town Centre Drive, Suite 200
Eagan, MN 55123
Phone: 651-255-3462
Fax: 651-255-3461

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Re: AW: [Histonet] Re: [Histology] Congo Red Stain

2012-11-08 Thread Rene J Buesa
For a strong staining with Congo Red and nuclear staining with hematoxylin, do 
the hematoxylin first of all steps and later stain for Congo Red.
Doing the hematoxylin last will produce weak nuclear staining.
René J.



From: Gudrun Lang gu.l...@gmx.at
To: 'Sherwood, Margaret' msherw...@partners.org 
Cc: Histonet@lists.utsouthwestern.edu 
Sent: Thursday, November 8, 2012 11:32 AM
Subject: AW: [Histonet] Re: [Histology] Congo Red Stain

We use the Merck kit but have turned the order of reagenses: first congored
and differentiation, then hemalaun. That masks much of unwanted staining.
Gudrun

-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Sherwood,
Margaret
Gesendet: Donnerstag, 08. November 2012 16:01
An: 'histonet@lists.utsouthwestern.edu'
Betreff: [Histonet] Re: [Histology] Congo Red Stain

To all:

Would people please share their method for doing Congo Red staining?  (We
are not interested in making up the reagents from powder form).

Thanks!
Peggy


Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherw...@partners.org



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the
e-mail contains patient information, please contact the Partners Compliance
HelpLine at http://www.partners.org/complianceline . If the e-mail was sent
to you in error but does not contain patient information, please contact the
sender and properly dispose of the e-mail.


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[Histonet] RE: Re: [Histology] Congo Red Stain

2012-11-08 Thread Lynette Pavelich
We used to use the traditional Congo Red for Amyloid, but our docs were not 
happy with the color whether purchased from a kit or manually made. We searched 
and tried Amyloid Red from Anatech, and have been doing it ever since. It is 
a brighter red color. Nothin' like a happy doc!

Lynette

Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI 48503

ph: 810.262.9948
mobile: 810.444.7966


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Sherwood, Margaret 
[msherw...@partners.org]
Sent: Thursday, November 08, 2012 10:00 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Re: [Histology] Congo Red Stain

To all:

Would people please share their method for doing Congo Red staining?  (We are 
not interested in making up the reagents from powder form).

Thanks!
Peggy


Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherw...@partners.org



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.


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[Histonet] RELIA Histology Careers Bulletin 11-8-2012 A mid-week update.

2012-11-08 Thread Pam Barker
Hi Histonetters!
How are you?  I wanted to drop you a quick line mid-week and let you know
about some of the positions I am working on.  
I have a number of really great opportunities For ASCP certified or eligible
histologists.
All of these positions are full time permanent and offer excellent pay,
great benefits and in some cases bonuses.  

The great thing about these opportunities is that if you are ready to move
right away they are too and if you want to pursue a position with a start
date after the holidays that works as well !!

RELIA Histology Spotlight Opportunity:
Histotechnician/Histotechnologist for a growing lab in Augusta, GA.  
This is a full time permanent position early morning shift M-F.  They offer
outstanding benefits a great group of people to work with and opportunity
for advancement!

Here is a list of some of our other current openings that I am excited
about!
*   Augusta, GA - Histotechnician/Histotechnologist  HT/HTL required
*   Harrisonburg, VA - Histotechnician Full time days HT required 
*   Charlotte, NC - Growing Hospital based lab All shifts. HT/HTL req.
*   Nashville, TN - Private Path Lab Afternoon shift  ASCP HT/HTL or
eligible
*   Collegeville, PA New Lab - Day shift HT/HTL and CLIA qual to gross
*   Reading, PA - In Office Path Lab - Dermpath Histotech Day Shift
HT/HTL and CLIA qual to gross
*   Dallas, TX - Private Reference Lab - IHC Tech ASCP HT/HTL, QIHC
preferred.
*   Portland, ME - Private Reference Lab - Histotech ASCP HT/HTL or
eligible
*   Topeka, KS - Lead Histotech/Supervisor ASCP HT/HTL 
*   Long Island, NY - Electron Microscopy Lead Tech/Tech - NYS lic or
elig.  

If you are interested in participating in my referral program  in addition
to histotechs for these positions I also need cytotechs in TN and SD.

If you or anyone you know might be interested in any of these opportunities
or would like help with a job search in another area of the USA please
contact me.  I can be reached at rel...@earthlink.net or toll free at
866-607-3542.



Thank You!
  
 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com http://www.facebook.com/PamBarkerRELIA /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 


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[Histonet] Gonadotrophin releasing hormone

2012-11-08 Thread Cheryl
Hi everyone-
 
Would you help me find a new vendor for this antibody:
 
Gonadotrophin-Releasing Hormone Receptor, Moust monoclonal.
 
It was vended by Vector Labs out of Burlingame CA as VP-G811.  
 
We're in the MIDDLE of a research study and having to change antibodies is 
going to be tough!
 
The original vendor said it was discontinues with no further information.  
Usually vendors can tell us why and I can't dig up anything more on 
this--hoping maybe they lost a contract or something where we can find it 
elsewhere...any help is VERY much appreciated.  
 
Cheryl

Cheryl Kerry, HT(ASCP) 
Full Staff Inc. 
Staffing the AP Lab by helping one GREAT Tech at a time.  
281.852.9457 Office
800.756.3309 Phone  Fax 
ad...@fullstaff.org 

Sign up for the FREE newsletter AP News--updates, tricks of the trade and 
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to apn...@fullstaff.org. Please include your name and specialty in the body of 
the email.
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[Histonet] BioInventory (Pathology) software recommendations?

2012-11-08 Thread Hugh Luk

Hi all,

Can I ask for recommendations for a Tissue block repository software?  It needs 
to incorporate FFPE blocks and their usage in research projects like TMA 
construction or RNA/DNA testing.  We will be most impressed with ease of use 
and pre-built categories with barcoding.  It must be able to incorporate ~15 
million entries.

Communication with CoPath/Cerner is a plus.

We have tried various companies, and are currently using one of them, but the 
software was not designed to capture workload and is a chore to use.

I would appreciate any advice.  Vendors welcome.


Thank you in advance,
Hugh
Hawaii



  
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[Histonet] Re: Congo Red Stain

2012-11-08 Thread Bob Richmond
If you're new to the congo red stain for amyloid:

You must have a positive control. Most of the controls I've seen
recently have been medullary carcinomas of the thyroid, which usually
(though not always) contain stainable amyloid.

I've asked many times on Histonet and never got a reply: amyloidosis
is fairly easily produced in experimental animals. Why isn't this
material used by pathologists as an amyloid control?

Supposedly paraffin sections of control tissue must be cut within a
month of use - the undeparaffinized sections deteriorate, though the
paraffin block themselves do not.

You must examine controls and patient sections using polarization. If
you don't have a polarizer on your microscope - and most pathologists
don't - then don't do the stain. The standard jiss - jiss use a pair
of broken sunglasses - is not satisfactory.

Anatech offers a stain they call Amyloid Red (it's clearly
characterized in their promotional material) - has anyone used it?

Bob Richmond
Samurai Pathologist
Maryville TN

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[Histonet] Stain for osteoporotic bone in Spurr plastic

2012-11-08 Thread Malandra, Allison E
Hi there -

  We are trying to stain osteoporotic bone that was embedded using Spurr 
plastic.  We need to be able to differentiate osteoid from mineralized bone.  
We have tried Gio's trichrome and Goldner's trichrome with no success, both 
surface staining and on deplasticized slides.  We are thinking of trying Von 
Kossa, does anyone have a recipe/protocol for this?  Or other types of stains 
that you have had success with?  Thank you!

Allison Malandra, DVM
University of Iowa, Bone Healing Research Lab



Notice: This UI Health Care e-mail (including attachments) is covered by the 
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this communication is strictly prohibited.  Please reply to the sender that you 
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RE: [Histonet] Stain for osteoporotic bone in Spurr plastic

2012-11-08 Thread Jack Ratliff
Allison,
I am not exactly certain if this protocol will work for Spurr's embedded 
specimens, but here is the protocol that I use for deplasticized sections of 
methyl methacrylate embedded undemineralized bone (non-decalcified):
Xylenes - warmed @ 50 C for 30 min w/ dip  dunk @ 15 min interval
Xylenes - warmed @ 50 C for 30 min w/ dip  dunk @ 15 min interval
Xylenes - warmed @ 50 C for 30 min w/ dip  dunk @ 15 min interval
100% EtOH - room temp for 5 min
95% EtOH - room temp for 5 min
70% EtOH - room temp for 5 min
DI H2O - room temp for 5 min
Silver Nitrate (20 g) + DI H2O (400 ml) - room temp (in dark protected from 
light) for 5 min
DI H2O rinse - room temp (protected from light) for 1 min
DI H2O rinse - room temp (protected from light) for 1 min
DI H2O rinse - room temp (protected from light) for 1 min
Sodium Carbonate (22.5 G) + DI H2O (337.5 ml) + 37% Formaldehyde (112.5 ml) - 
room temp (protected from light) for 2 min
DI H2O rinse - room temp for 1 min
DI H2O rinse - room temp for 1 min
Sodium Thiosulfate (40 g) + Potassium Ferricyanide (2 g) + DI H2O (420 ml) 
Solution - room temp for 30 sec(solution stable for 30-60 min after addition of 
potassium ferricyanide)
Running Tap Water Rinse - 10 min
Counterstain w/ MacNeal's Tetrachrome (12 g) + DI H2O (600 ml) - room temp for 
6-8 min(stir continuously w/ heat @ 60C for 48 hours covered, then filter with 
paper towel)
DI H2O rinse - room temp for 1 min
DI H2O rinse - room temp for 1 min
DI H2O rinse - room temp for 1 min
70% EtOH - room temp for 1 min
100% EtOH - room temp for 1 min
Xylenes - room temp for 3 min
Xylenes - room temp for 3 min
Coverslip w/ Eukitt


Hope this helps! Please feel free to contact me if you have any questions or 
concerns.
Best Regards,
Jack

Jack L RatliffOwner, Ratliff Histology Consultants, LLCChairman, Hard Tissue 
Committee - National Society for Histotechnology
(317) 281-1975jratl...@ratliffhistology.com LinkedIn Profile:  
http://www.linkedin.com/profile/edit?trk=hb_tab_pro_top




 From: allison-malan...@uiowa.edu To: histonet@lists.utsouthwestern.edu
 Date: Thu, 8 Nov 2012 22:52:42 +
 Subject: [Histonet] Stain for osteoporotic bone in Spurr plastic
 
 Hi there -
 
   We are trying to stain osteoporotic bone that was embedded using Spurr 
 plastic.  We need to be able to differentiate osteoid from mineralized bone.  
 We have tried Gio's trichrome and Goldner's trichrome with no success, both 
 surface staining and on deplasticized slides.  We are thinking of trying Von 
 Kossa, does anyone have a recipe/protocol for this?  Or other types of stains 
 that you have had success with?  Thank you!
 
 Allison Malandra, DVM
 University of Iowa, Bone Healing Research Lab
 
 
 
 Notice: This UI Health Care e-mail (including attachments) is covered by the 
 Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential 
 and may be legally privileged.  If you are not the intended recipient, you 
 are hereby notified that any retention, dissemination, distribution, or 
 copying of this communication is strictly prohibited.  Please reply to the 
 sender that you have received the message in error, then delete it.  Thank 
 you.
 
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[Histonet] SOX-10

2012-11-08 Thread Chakib Boussahmain
Hi Histonet,
Does anyone using SOX-10 antibody? if so can you share with me the protocol? 
dilution?
Thank you so much.
Chakib Boussahmain
HTL(ASCP)
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