[Histonet] Sugar cane
Morning everyone, Does anyone have a protocol for the processing of sugar cane they can share? Thanks much! Jeanine H. Bartlett, BS HT(ASCP), QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, NE MS/G-32 Atlanta, Ga 30333 404-639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Sugar cane
Into Rum? That might be illegal without a permit. :o) I apologize in advance but I couldn't resist a little attempt at humor on this one. Cheers, Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewal...@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition® and the Governor's Award for Performance Excellence -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID) Sent: Friday, November 16, 2012 8:40 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Sugar cane Morning everyone, Does anyone have a protocol for the processing of sugar cane they can share? Thanks much! Jeanine H. Bartlett, BS HT(ASCP), QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, NE MS/G-32 Atlanta, Ga 30333 404-639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sugar cane
The only thing difficult about sugar cane (I imagine the stems) is that it contains silicon spikes that can be very difficult to section because those fibers can affect the edge of the sectioning blades. Other than that you can use any method for plant tissues. René J. From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Friday, November 16, 2012 8:40 AM Subject: [Histonet] Sugar cane Morning everyone, Does anyone have a protocol for the processing of sugar cane they can share? Thanks much! Jeanine H. Bartlett, BS HT(ASCP), QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, NE MS/G-32 Atlanta, Ga 30333 404-639-3590 jeanine.bartl...@cdc.hhs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] procedure for destaining a cytology slide followed by restaining with a special stain, such as PAS?
Does anyone have a procedure for restaining a cytology slide? We experienced a loss of cells on an FNA aspirate of a lymph node, which had been originally stained with Diff Qwik. The procedure we used was as follows: 1) Destain using 1% Acid Alcohol (1 ml. concentrated HCL added to 99 ml. 70% alcohol) for approx. 30-60 seconds. 2) Restain on automated stainer (Ventana) with PAS kit. Thanks, Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] High Complexity testing
Does anyone know where to find the High complexity testing requirements for CAP? We're about to apply for CAP accreditation and want to make sure the person working in our lab grossing has the requirements. Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] holes in derm tissue sections
Generalized Holes commonly present with one of two scenarios. Scenario One-a Histotech may be sloppy about allowing sections to dry thoroughly before warming or baking. Scenario Two is generalized, showing up with slides from every Histotech, and representing unstabilized foci where paraffin did not penetrate, eg a processing failure. Label it as a QI event, call it catastrophic tissue loss. With that mindset, Examine slides microscopically. Establish baseline: Measure one whole day by examining every slide and counting how many have holes or unstable collagen. Secondarily Pay attention to/ record tissue size. Simple Remedies-Three processing areas to examine. Change your processing alcohols, using NEW, not recycled. Change your xylenes, NEW, not recycled. Rotate paraffins and Lengthen time in last clean paraffin by 25%. Measure again. Examine the remaining failures. catastrophic tissue loss may sound a bit harsh, but I have special fondness for derm and hold them to a higher standard. Derm labs which cannot reliably find satellite metastasis of melanoma are XXX007. So label it for what it is- catastrophic tissue loss. Then put your foot on the gas and fix it urgently, and follow-up those remaining failures. Even if long-term you change your processing program or process according to size or take formalin off your processors. or more drastically, change behavior among grossers to assure proper fixation and slicing of excision specimens. #4 POST-FIX large blocks, those 10mm after gross slicing to proper thickness, place cassettes in fresh formalin for several hours. Change formalin on grossing bench daily. If you post-fix on the tissue processor, change or rotate formalin daily. Dirty formalin smells bad but does not fix well. The sniff test is inaccurate for determining formalin activity. #5 Spreading out cassettes in processing baskets allows for more diffusion. Not more than two high. Stay with it Steve Steve A. McClain, MD McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Nerve teasing videos?
Hi all, does anyone know of instructional videos for nerve teasing methods? I tried Youtube but didn't find anything... Thanks in advance! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 88305TC starting to hit the fan...
The news about the decrease of the 88305TC medicaire reimbursement fee was just announced at my lab finally. This of course struck fear in the entire lab workforce because it's clear some major belt-tightening will have to be done, and it's most likely to hurt everybody. Costs and probably hours will have to be reduced it seems clear. Isn't the finaly figure for this cut (%) supposed to be announced today? I have heard anywhere from 20 to 52% is possible? This will give a final measure as to how many belt loops we'll have to cinch-in... Anybody else out there starting to feel the pain in their labs yet? FREE ONLINE PHOTOSHARING - Share your photos online with your friends and family! Visit http://www.inbox.com/photosharing to find out more! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Nerve teasing videos?
If the videos exist, I'm very interested in this as well. Thanks! Lucie Lucie Guernsey UCSD Dept. of Pathology lguern...@ucsd.edu On Fri, Nov 16, 2012 at 10:11 AM, Morken, Timothy timothy.mor...@ucsfmedctr.org wrote: Hi all, does anyone know of instructional videos for nerve teasing methods? I tried Youtube but didn't find anything... Thanks in advance! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] University of Arkansas for Medical Sciences Has Two HT or HTL Positions Open
We have two positions open for either a registered HT or HTL at UAMS. We are in Little Rock Arkansas in the middle of the state and it is a gorgeous area. We have great benefits and a competitive salary range. We are currently reorganizing the Histology Laboratory and seeing increases in our workload. This is a modern laboratory with up to date equipment and more changes coming. Join us for a new direction in your career and future. Please e-mail if you are interested and we will set up an interview either by phone for those out of town or in person after your application has been processed through our HR. (Please e-mail me first as they tend to not get all qualified applicants to us.) Please note recruiters need answer this as we are not allowed to use your services and it would be a waste of your time and energy. (Wish we could - the state says NO) Best Regards, Pamela A Marcum AP Supervisor Histology Slot 502 4301 W Markham Street Little Rock AR 72205 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
